Objective To investigate the relationship between mine environment and hypertension in miners. Methods 1736 male miners who worked under the ground and 825 on the ground were recruited in this study. Prevalence of hypertension under the ground and on the ground miners was compared. Results Prevalence of hypertension of miners under the ground was 23.91% and on the ground was 15.52% (χ2 = 23.56,P <0.001 ). Compared to miners on the ground, the relative risk of hypertension under the ground workers was 1.71 (95% CI 1.38 - 2.13 ). Prevalence of hypertension was correlated to the years of ground working (χ2 = 37.00, P < 0.001 ). The binary logistic regression showed significant relationship between mine environment and hypertension under the ground miners ( OR = 1.05,95% CI 1.02 - 1.08 ).Conclusion The underground environment is an important risk factor hypertension to the miners.

Objective To analyze distribution and drug resistance of MRSA separated,to probe into a treat method for MRSA infection and offer scientific gist for reasonable use of clinical antibiotic. Methods 568 MRSA separated from clinic were measured and a drug sensitivity test was performed by means of Kirby-Bautr agar diffusion method. Results 370 individual plants were filtered, the rate of inspection was 65.1%. MRSA was 36.2 % among them,MRSCON was 77.5% ,the drug resistance of MRSA to quinolones(21% ～48% ) was below MRSCON(84 %～89 % ). The drug resistance of MRSA and MRSCON obviously excelled MSSA and MSSCON ( P＜0.05 ). VRS was undiscovered. Conclusion Severe infection caused by MRS should choose vancomycin resistant enterococci to cure at fist. Staphylococcus was one of primary pathogenic bacterium caused infection in hospital, MRS had multiple drug resistance and measure range about drug resistance should be extended, which would advance the cognition about the drug resistance of antibiotic and cut off the transmitted path, reduce the drug resistance of bacteria and the infection rate in hospital.

Objective To observe clinical symptoms and the length of hospital stay of patients with bronchiolitis under different treatment,and the curative effect of inhalation of hypertonic saline combined with epinephrine in the treatment of bronchiolitis.Methods A total of 158 patients with bronchiolitis under 2 years old between August 2012 and May 2013 in Shengjing Hospital of China Medical University were enrolled.According to the admission dyspnea score (respiratory distress assessment instrument,RDAI),all cases were divided into two major groups,moderate group and severe group.Two major groups were randomly divided into three groups (treatment group 1,2,3)according to the different treatments respectively.Patients in the treatment group 1 were inhaled pulmicort combined with combivent,treatment group 2 were inhaled hypertonic saline,and treatment group 3 were inhaled hypertonic saline combined with epinephrine.The symptoms and signs disappeared days,respiratory assessment change score,length of hospital stay and the curative effect in every group were observed before and after the treatment.Results In the moderate group,there were no significant differences in the length of time of cough,wheeze,lung rales disappears as well as the average length of hospital stay between before and after treatment in three treatment groups respectively (P ＞0.05,respectively).In the severe group,there were significant differences in the respiratory assessment change score and length of hospital stay of treatment group 2 and treatment group 3 than that of treatment group 1 respectively(P ＜ 0.05,respectively).There were no significant differences in the length of hospital stay and length of time of cough,wheeze,lung rales disappears between treatment group 2 and treatment group 3 respectively(P ＞ 0.05,respectively).Conclusion There were no significant differences in the curative effect between each treatment group for the moderate bronchiolitis patients.For the severe bronchiolitis patients,hypertonic saline combined with epinephrine inhalation had better curative effect,less adverse reactions.

Objective To investigate the effects of vasoactive intestinal peptide on transforming growth factor-β1(TGF-β1)expression in lung tis-sue of asthmatic mice with airway remodeling. Methods A total of 30 SPF grade female Balb/c mice were randomly divided into three groups:con-trol(group A),asthmatic(group B),and vasoactive intestinal peptide treatment(group C). Aerosolized ovalbumin was used to establish the asth-matic mice model. Pathological changes of bronchial and lung tissues were observed by HE,the perimeter of basement membrane(Pbm),internal wall area(WAi)and smooth muscle area(WAm)in bronchial lung tissue were analyzed using the pathological image. The expression levels of TGF-β1 mRNA and protein in lung tissue were detected by real-time PCR and Western blot. Results Pathologic changes including collagen deposition,in-filtration of inflammatory cells and smooth muscle layer thickness were found in group B. In addition,the WAi/Pbm and WAm/Pbm were significant-ly higher in group B than those of the group A(P<0.05),and lower in group C than in group B(P<0.05). The expression levels of TGF-β1mRNA and protein in group B were higher than both group A(P<0.05)and group C(P<0.05). Conclusion Vasoactive intestinal peptide may inhibit airway remodeling,which may be associated with reduced expression of TGF-β1.

Based on analyzing the problems existed in hostital informatization, the paper proposes using shared-database to realize data sharing among various departments.It mainly introduces shared-database and its function module and customer display module. The introduction of shared-database is conducive to comprehensive management of hospital data, providing basis for future data mining.

Objective to study the effect of curcumin on tumor necrosis factor α(tNF-α)-induced osteoblast apoptosis. Methods Primary osteo-blast cells were divided into three groups:control group(no treated),group treated with tNF-α(treated with tNF-α for 24 h),and group pretreated with curcumin(treated with curcumin for 2 h,and then treated with tNF-α for 24 h). Western blot analysis was carried out with primary antibodies against caspase-2,caspase-3,caspase-8,Bcl-2,Fas,and tRAIL. Apoptosis cells were observed by immunofluorescence microscope. Results In group treated with tNF-α,the protein levels of caspase-2,caspase-3,caspase-8,Fas,and tRAIL increased(P < 0.01),whereas the protein level of Bcl-2 remained unchanged(P > 0.05). In group pretreated with curcumin,the protein levels of caspase-2,caspase-3,caspase-8,Fas,and tRAIL were lower than those in group treated with tNF-α(P < 0.01). the number of apoptotic cells was dramatically increased after the administration of tNF-α. While the number of apoptotic cells decreased in the presence of curcumin. Conclusion the present study clearly demonstrated that cur-cumin can suppress osteoblast apoptosis induced by tNF-α.

Objective To investigate the protective effects of polysaccharide from Radix Codonopsis Lanceolatae on acute alcoholic hepatic injury in mice.Methods The model of CCl4-induced acute hepatic injury was set up in mice, and then treated with polysaccharide from Radix Codonopsis Lanceolatae to observe the activities of ALT, AST and TG in serum and the levels of SOD, GSH and MDA in hepatic tissue and Observed the pathological changes in the liver of mice.Results Compared with model control group, the lance asiabell root polysaccharide liver injury in mice all dose groups in the activity of serum ALT(97.5 ± 22.12 U/L, 108.8 ± 22.15 U/L, 125.2 ± 19.68 U/Lvs. 152.3 ± 17.32 U/L), AST (72.5 ± 21.3 U/L, 89.2 ± 17.47 U/L, 104.3 ± 19.34 U/Lvs.1215 ± 22.31 U/L) and TG(0.75 ± 0.32 mmol/L, 0.80 ± 0.22 mmol/L, 0.96 ± 0.21 mmol/L vs.1.44 ± 0.23 mmol/L) reduce, can increase the liver damage the activity of SOD(215.6 ± 22.68 U/mg, 203.2 ± 22.18 U/mg, 186.2 ± 18.34 U/mgvs.164.3 ± 17.54 U/mg), GSH(17.74 ± 2.12μmol/g, 14.84 ± 1.12μmol/g, 13.93 ± 2.15μmol/gvs.12.23 ± 2.09μmol/g) in liver tissue of mice, decrease the activity of MDA(6.22 ± 2.12 nmol/mg, 6.22 ± 2.12 nmol/mg, 8.36 ± 1.84 nmol/mgvs.9.35 ± 1.68 nmol/mg) in liver tissue, showing significant differences (P<0.05), and there was certain dose dependent, and can improve the pathological injury of liver tissue.Conclusion Polysaccharide from Radix Codonopsis Lanceolatae had protective effects on acute liver induced by acute alcoholic hepatic injury in mice.

It has been found by sorting out and analyzing ancient and modern doctors’ green tortoise exploring point acupuncture manipulation that ancient and modern doctors use Xu Feng's manipulation as the foundation and make some innovations on that b asis and according to their own clinical experiences. The clinical application range of different doctors’ green tortoise exploring point acupuncture manipulation is proposed by summarizing recent over 10 years’ clinical reports and the authors’ clinical practice. It is considered that the shortcomings of green tortoise exploring point acupuncture manipulation are lack of manipulation standardization and modern mechanism study and that manipulation standardization should be closely connected with clinical practice for further application and popularization.

OBJECTIVE:To explore the intelligent management of drugs within expiry dates.METHODS:Table of drug categories was established;those categories near expiry date were screened out automatically through the paste function of Excel.RESULTS:Expiry dates of all drugs were seen clearly.CONCLUSION:It is accurate and simple by applying excel in the intelligent management of drugs within expiry dates.

OBJECTIVE:To optimize matrix composition and technique for acne emulsion.METHDOS:Oil phase,emulsifier and emulsify temperature were selected as variable factors using orthogonal design method,and table L 9 (3 4 )was used for the experiment.RESULTS:The optimum matrix composition and technique were stearic acid of15g,liquid paraffin of13g,vaseline of20g,trietha nolamine of3g,emulsifying temperature of85℃.CONCLUSIONS:The acne emulsion prepared by this kind of composition and technique accord with the stipulation of Chinese Pharmacopoeia.