Objective To investigate the application value of contrast-enhanced ultrasound in the diagnosis of ovarian masses.Methods Ninety-four patients with ovarian masses were observed and undetermined by conventional ultrasound examinations who were underwent contrast-enhanced ultrasound examinations and were made the time-intensity curve.By analyzing the perfusion characteristics and the quantitative parameters of time-intensity curve,we compared the difference of different masses.Results The perfusion characteristics and the quantitative parameters of the time-intensity curve were different.The arrival time and the time to peak intensity of benign masses were later than those of malignant tumors.The peak iniensity of benign masses was lower than that of malignant tumors.There was a significant difference between the two groups.The arrival time and the time to peak intensity of benign tumors were later than those of malignant tumors.The peak intensity of benign tumors was lower than that of malignant tumors.There was a significant difference between the two groups.The time to peak intensity of non-tumorous lesions was later than that of malignant tumors.The peak intensity of non-tumorous lesions was lower than that of malignant tumors.There was a significant difference between the two groups.The arrival time of non-tumorous lesions was earlier than that of benign tumors.There was a significant difference between the two groups.Conclusion The perfusion characteristics and the quantitative parameters that draw from the time-intensity curve of different masses are different.Contrast-enhanced ultrasound is contributive to the diagnosis and the differential diagnosis of different masses.Contrast-enhanced ultrasound also has great clinical values to those ovarian masses whose ultrasonic appearance is complex and difficult to diagnose qualitatively.

Recent reports have indicated that Statins could remarkably improve the prognosis of patients suffered from coronary artery disease.And this effect is not only due to Statin's lipid-regulating function,but also correlated to its non-lipid-regulating action.Atorvastatin is the latest kind of statins.In this article,we specified the non-lipid-regulating effect of Atorvastatin in the prevention and therapy to coronary artery disease.Furthermore,we summarized its corresponding clinical evaluation or security.

Objective　To investigate the level of cell adhesion molecule sialyl-LeX expression in colorectal carcinoma and its relation with carcinogenesis, differentiation, metastasis and prognosis. Methods　Sialyl-LeX expression and its optical density in colorectal carcinoma (n=90) and remote normal mucosa (n=30) were quantitatively studied with microwave-LSAB immunohistochemical method combined with image analysis technique. Fifty-three patients were followed up. Results　 The weaker staining in remote normal colorectal mucosa was observed in very limited parts of some deep crypts. Positive rate of sialyl-LeX expression was only 16.7%(5/30). The positive expression of sialyl-LeX was observed in 83 of 90 patients with colorectal carcinoma(92.2%). The apical cytoplasma of cancer tubules, the luminal contents, and the cytoplasma of the cancer cells were strongly stained. The mean integral optical density of sialyl-LeX positive cell in poorly differentiated adenocarcinoma was significantly higher than that in highly differentiated and mucinous ones (P<0.01). It was markedly higher in patients with positive lymphatic nodes than that in negative ones (P<0.01). With followed-up for longer than 5 years, it was much lower in the alive cases than that in the dead (P<0.01). Conclusion　These findings indicate that changes of sialyl-LeX expression and its optical density is related to carcinogenesis, differentiation, invasion and metastasis of colorectal carcinoma. It may be a good predicter for the prognosis of patients with colorectal carcinoma.

Objective To discuss the related factors about the rising cesarean section rates, and to propose feasible measures to reduce cesarean section rates. Methods Cesarean section cases in our hospital from January, 2000 to December, 2009 were retrospectively analyzed. Results The cesarean section rate increased year by year,32.0% in 2000,34.5% in 2001,35.0% in 2002,32.3% in 2003,37.1% in 2004,37.9% in 2005,40.2% in 2006,41.7% in 2007,43.6% in 2008,46.1% in 2009. Conclusions Effective measures should be taken to reduce the cesarean section rate.

Objective To observe the effect of exercise on insulin resistance induced by a high fat diet and to explore the underlying mechanism.Methods Thirty healthy male Wistar rats were randomly divided into a normal control group (NC group) and a high-fat group (HF group),fed with normal chow and a high fat diet respectively.Eighteen weeks later,insulin resistance had appeared in the HF group.The HF group was then randomly subdivided into a high-fat diet control group (HC group,fed a high fat diet) and an exercise group (HE group,fed a high fat diet and subjected to swimming training for 6 weeks).After 24 weeks,an insulin sensitivity index was calculated.Serum free fatty acid (FFA) and skeletal muscle triglyceride (TG) were detected.Phosphorylation of adenosine monophosphate kinase-α (AMPKα) in skeletal muscle was detected by Western blotting.Results At twenty-four weeks the insulin sensitivity of the HC group had decreased significantly compared to the NC group.Serum FFA level and skeletal muscle TG content had both increased.Average phosphorylation of AMPKα in the HC group decreased to 48.7% of the NC group average.Compared to the HC group,insulin sensitivity and phosporylation of AMPK-α were elevated significantly in the HE group.Serum FFA level and skeletal muscle TG content were both lower.However,compared to the NC group,both serum FFA level and skeletal muscle TG content had increased in the HE group,while insulin sensitivity and phosphorylation of AMPKα had significantly decreased.Conclusions Exercise can significantly improve insulin resistance induced by a high-fat diet through decreasing serum FFA level and ectopic li-pid accumulation in skeletal muscle.

Objective To investigate the changes of intestinal mucosal barrier function and protection of alanyl-glutamine(Alm-Gln) dipeptide during early stage of postthoracotomy in aged patients.Methods A prospective,randomized and controlled trial was conducted and 20 aged patients who underwent non-digestive thoracotomy were randomized into two groups,experimental group (intravenous administration of Aln-Gln dipeptide,0.5 g/(kg · d),for 4 days,n =10) and control group(equal amount saline as placebo,for 4 days,n =10).The indices of boby temperatures,heart rates,respiration and white blood cell count of all patients were daily recorded during administration.Serum concentrations of glutamine (Gln),D-lactate,diamine oxidase (DAO) and tumor necrosis factor-α(TNF-α) were measured before and after operation.Results There were no statistically significant differences in the patients' general information between experimental group and control group including age,gender and body weight.Plasma Gln concentration in postoperative 5 days was higher than that of pre-operation of experiment group ((478.32 ± 47.42) μmol/L vs.(372.67 ± 29.14) μmol/L,P =0.021).The plasma Gln level of control group at 5th day after operation was higher than that in pre-operation ((431.12 ± 42.27) μmol/L vs.(386.29 ± 19.73) μmol/L,P =0.017).The plasma level of Gln in experimental group was significantly higher than that in control group after operation((478.32±47.42) μmol/L vs.(386.29 ± 19.73) μmol/L,P =0.012).There were no significantly differences between the two groups in terms of the plasma level of DAO and D-lactate before operation (P ＞ 0.05).Meanwhile the levels of DAO and D-lactate in both group at 5th day after operation were significantly higher than that at before operation(DAO:(2.53 ±0.47) U/ml vs.(1.66±0.32) U/ml,P =0.003;D-lactate:(6.82 ±1.91) mg/L vs.(4.92 ±1.57) mg/L,P =0.024),and the levels of them in experimental group were significantly lower than that in control group(DAO:(1.10 ± 0.23) U/ml vs.(2.53 ± 0.47) U/ml,P =0.013 ; D-lactate:(4.87 ± 1.33) mg/L vs.(6.82 ± 1.91) mg/L,P =0.019).The concentration of TNF-α was significant increase in both two groups at first day after operation,but decreased at the third day.The concentration of TNF-α in experimental group at 5th day after operation was lower than that in control group ((6.89 ± 5.21) pg/L vs.(13.04 ± 4.46) pg,/L,P =0.003).The morbidity of systemic inflammatory response syndrome (SIRS) was significantly decreased in experimental group and the rate of SIRS was also lower than that in the control group(P ＜ 0.01).Conclusion Intestinal mucosal barrier function was damaged after thoracotomy in aged patients.Administration of Aln-Gln dipeptide could increase the level of serum Gln,protect the intestinal barrier and attenuate the systemic inflammatory response.Aln-Gln dipeptide can be used to help aged patients recover rapidly.

Objective To investigate the influence of Jianpi Bufei Huatan Recipe (JBHR), a Chinese herbal compound with the actions of strengthening spleen, tonifying lung and resolving phlegm, on the pulmonary function of childhood bronchial asthma at remission stage, and to explore the possible therapeutic mechanism. Methods Sixty bronchial asthma children at remission stage were divided into treatment group and control group, 30 cases in each group. Both groups were treated with inhalation of fluticasone propionate, and additionally, the treatment group was given oral use of JBHR(mainly composed of Radix Astragali, Radix Pseudostellariae, fried Rhizoma Atrctylodis Macrocephalae, Radix Saposhnikoviae, Poria, Pericarpium Citri Reticulatae, Rhizoma Pinelliae, prepared Radix Glycyrrhizae, calcined Os Draconis, calcined Concha Ostreae, and Semen Lepidii seu Descurainiae). The therapeutic effect of the two groups was evaluated after treatment, and the changes of peak expiratory flow(PEF), peak expiratory flow variation(PEFv) were also observed after treatment for 1, 2, 3, 6, 9, a nd 12 month(s). Results (1)The total effective rate of the treatment group was 93.33%, and that of the control group was 83.33%, the difference being significant(P<0.05). (2) During the treatment, PEF was higher and PEFv was lower in the treatment group than those in the control group(P<0.05). Conclusion JBHR could relieve the clinical symptoms, improve the pulmonary function and decrease the hyperactivity of airway of pediatric bronchial asthma at remission stage with lung-spleen qi deficiency.

Sepsis is defined as an organ dysfunction caused by systemic inflammatory response induced by infection,and may eventually developed into multiple organ failure (MOF).The onset of sepsis often involves the activation of inflammatory cells,over expression of inflammatory cytokines,immune dysfunction,damage of tissue and organ,excessive apoptosis of cell.Mesenchymal stem cells (MSCs) have a wide range of biological activities,including anti-inflammatory,antibacterial,immune regulation,regeneration and repair of tissue and organ,anti-apoptosis,etc.In recent years,a large number of preclinical studies had demonstrated that MSCs has good actions on sepsis.The purpose of this review is to describe the mechanism of MSCs in the treatment of sepsis,and to point out the possible clinical challenges.

OBJECTIVE:To establish a method for the determination of the dissolution rates of azithromycin tablets and to compare the dissolution rates among azithromycin tablets that from different manufacturers so as to provide references for clinical medication.METHODS:The concentrations and drug cumulative dissolution rates of a total of 5 batch numbers' azithromycin tablets that from 5 pharmaceutical factories were determined based on the related standard procedure indexed in China Pharmacopoeia(2005 edition),then the dissolution parameters were calculated with Weibull formula.RESULTS:The dissolution rates at 45 min were above 75%,which was up to the standard.The analysis of variance showed that there were significant differences among samples in dissolution parameters(P

Objective To investigate the expression and distribution of sialyl Lewis X in colorectal carcinoma and its relationship with carcinogenesis, progression and metastatic proclivity.Methods Microwave LSAB immunohistochemical technique was used to detect the expression of sialyl Lewis X in colorectal carcinoma and in normal mucosa. Immunoelectromicroscopic localization of sialyl Lewis X labelled by colloidal gold was also observed.Results The positive rate of sialyl Lewis X expression in primary colorectal cancer was 92 2%(83/90), and 16 7%(5/30) in normal mucosa. The positive rate was 100% in patients with lymphatic nodes metastasis, compared with that of negative nodes of 82 1% ( P