Animals ; Ascorbic Acid ; metabolism ; Brain Ischemia ; metabolism ; Glutamic Acid ; metabolism ; Hippocampus ; drug effects ; metabolism ; Male ; Propofol ; pharmacology ; Rats ; Rats, Wistar ; Reperfusion Injury ; metabolism

Objective To study the effect of cooperative network on in-job training of nurses. Methods 80 randomly selected nurses with length of service from 1 to 5 years participated the written examination with alternation of the network test monthly. Self- designed questionnaires were sent out to survey the nurses about the recognition of the two testing modes. Department of Nursing compared and analyzed the exam results. Results The network test was popular among nurses, besides, it was economical, convenient, could save human resources, contribute to remarkable learning effect. Conclusions Application of cooperative network test in in-service training of nurses is convenient and practical, and can improve collaboration and team spirit of nurses.

Nonalcoholic fatty liver disease(NAFLD)is the Clinicopathological syndrome characterized by diffuse hepatic steatosis from excessive use of alcohol and other specific factors that cause liver damage including simple steatosis,and nonalcoholic steatohepatitis.The pathogenesis of NAFLD is closely related to Insulin resistance and genetic susceptibility.Presently the major treatment of NAFLD is diet control to reduce weight.On the basis of the treatment with dyslipidemia and hypoglycemic drugs or application of weight loss for more than one month,are there still mixed hyperlipidemia or hyperlipidemia with two or more risk factors,need to add Lipid-lowering drugs such as fibrates,statins or Probucol should be considered.The prevention and treatment with lipid-lowering drugs in NAFLD will be summarized in this article.

Objective To investigate the blood cell separator collection of peripheral blood stem cell after using recombinant human granulocyte colony - stimulating factor (rhG - CSF) to mobilize the normal donor and patients with hematologic malignancies, through morphological observation of lymphocyte ratios and PBSC vitality of mononuclear cells. Methods Peripheral blood mononuclear cells (MNC)were collected from 15 cases of healthy donors and 14 patients of hematologic malignancies with rhG - CSF mobilization dose with blood cell separators. The number of nucleated cells, classification of nucleated cells, MNC count and cell viability were observed. Results ①The total number of nucleated cells in normal donor group was(4.31?1.41)?10~8 / kg,and hematologic malignancies group was (6.21?4.37)?10~8/kg. ②The number of nucleated cells of PBSC in hematologic malignancies group was higher than those of the donor group (P0.05).④MNC number was (96.7? 5.1) % in normal donor group counts, and in which lymphocytes was (65.9?9.4)% , myeloid (16.3?8.7)% , mononuclear cells (16.5?4.0)% ,respectively,MNC number was (92.7?15.6)% in hematologic malignancies group, and in which lymphocytes was (55.3?16.7)% , myeloid (24.3?16.5) % , mononuclear cells (14.9?9.1)%, respectively. ⑤Cell viability in normal donor group before and after cryopreservation were (91.5?4.3)%, (67.4?9.1) % , in hematologic malignancies grou were (82.9?11.1) % , (56.5?20.1) % , respectively. Cell viability was significantly different (P

Objective To study the impact on the proliferation and cytotoxicity of donor T cells during mobilization with rhG - CSF. Methods The peripheral blood mononuclear cells (PBMNC) were collected from 20 healthy donors before mobilization and on the fifth day of mobilization with rhG - CSF. After the PBMNC was activated with 500 ng/ml of CD3 monoclonal antibody and 500 ?g/ml of rhIL -2 for 96 hours,the activated T cells were collected for testing proliferation, cytotoxicity. Fas expression, perforin and Fas ligand (FasL) mRNA expression,and IFN -? concentration in the culture medium of the activated T cells, were determined by radioimmunoassay. Results The proliferation activity and cytotoxicity of T cells activated with CD3 monoclonal antibody and rhIL - 2 were reduced markedly after mobilization with rhG - CSF(P0.05). The activated T cells expressed perforin mRNA and didn' t express FasL mRNA both before and after mobilization with rhG - CSF. The concentration of IFN -? in the culture medium of the activated T cells decreased significantly after mobilization with rhG - CSF(P

Objective:To study the inhibitory effect of rosmarinic acid on xanthine oxidase.Methods: Xanthine oxidase((0.1 U/ml)) was incubated with xanthine(1 mmol/L for determining formation of uric acid;50 ?mol/L for determining superoxide anions) in the presence of 20,40 and 60 ?g/ml rosmarinic acid or allopurinol as positive control.The formation of uric acid was determined by automatic biochemical analyzer 5 min after reaction and the production of superoxide anions was measured by Nitro Blue Btetrazolium(NBT) reduction.HL-60 cells(1 ml,2?10~(5)/ml) were pretreated with xanthine(100 ?l,6 mol/L) and xanthine oxidase(100 ?l,0.1 U/ml),then rosmarinic acid(500 ?g/ml) or allopurinol(1 ?g/ml,as positive control)(AnnexinⅤ-PI kit) was added to determine the cell apoptosis rate.HL-60 cells(1 ml,2?10~(5)/ml) were also pretreated with xanthine(100 ?l,6 mol/L) and xanthine oxidase(100 ?l,0.1 U/ml),then rosmarinic acid(500 ?g/ml) or SOD(100 U/ml,as positive control)(cell cycle method) was added to determine the cell apoptosis rate.Results: Rosmarinic acid obviously inhibited the production of uric acid and superoxide anion-induced reaction in NBT assay,with their IC_(50)being 56 ?g/ml and 21 ?g/ml,respectively.The rates of apoptosis inhibition by rosmarinic acid were both over 40% by Annexin Ⅴ-PI kit and cell cycle method.Conclusion: Rosmarinic acid is a competitive inhibitor of xanthine oxidase.

Objective:To evaluate the effect of mustard gas on reproductive system in rats.Methods:Forty male SD rats and 40 female rats were equally divided into mustard gas-treated group and control group.Animals in mustard gas-treated group were injected with 5 mg?kg body weight(LD_(50))of mustard gas,and those in control group received propylene glycol injection.Serum hormonal determinations(estriol,testosterone,luteinizing hormone,follicle-stimulating hormone)were completed by collecting ocular vein blood at 1 day,3 days,5 days,7 days and 28 days after injection.The rats were then killed were anatomized.Microscope was used to observe pathological changes in germ cells(ovary or testes).Two female rats and 3 male rats died during 4th and 5th day after injection.Results: The changes of serum hormone and pathology nearly restored to the normal levels in the female rats after injection of mustard gas for a short period.Most of the spermatogenic cells were still necrotic and seminiferous tubules still had hollow cross 1 month after injection in male rats.The FSH levels in the sera of male rats were significangtly increased from(1.2100?0.3546)mmol/L in control group to (1.4750?0.0451)mmol/L 28 days after injection(P

The HIV/AIDS epidemic in China has entered the Widespread Epidemic Period.HIV-infected cases have been reportedly found in all 34 provinces(minority autonomous regions/municipalities).HIV is spreading from high risk group to general population.Our government has paid much attention to AIDS prevention and control,lots of effective measures have been carried out,and comprehensive prevention.treatment,bealth care and financial support have been launched.Relatively consummate surveillance system and laboratory testing network of AIDS have been established at present and large SCale of ARTs have been carried out through the whole country.There is a great change in the situation on AIDS Prevention and Control in China.

Objective To observe the clinical efficacy of acupuncture at phenomaxillary ganglia in treating dry eye syndrome (DES). Methods Totally 148 DES patients were randomized into a treatment group of 72 cases and a control group of 76 cases. The treatment group was intervened by acupuncture at phenomaxillary ganglia plus Jingming (BL 1), Sibai (ST 2), and Taiyang (EX-HN 5), while the control group was by using eye drops of artificial tears. The DES symptoms score and Visual Analogue Scale (VAS) were observed before and after intervention, and the clinical efficacies were compared. Results The total effective rate was 90.3%in the treatment group versus 68.4%in the control group, and the difference was statistically significant (P<0.01). The DES and VAS scores were significantly changed after intervention in both groups (P<0.01). The changes of DES and VAS scores in the treatment group after intervention were significantly different from those in the control group (P<0.05). Conclusions Acupuncture at phenomaxillary ganglia can go deep and directly reach the affected area, and thus is an effective method in treating DES.

Objective To investigate follow-up willingness of patients and their families after discharge.Method A follow-up willingness questionnaire for cancer patients and their families was designed,the items covering follow-up willingness,follow-up methods, follow-up time,follow-up demand for content etc.Results Ninety-three point nine percent of patients and 96.9%of families were willing to accept follow-ups;84.8%of patients and 81.3%of families were willing to accept follow-ups by phone calls;42.4%of patients and 43.8%of families were willing to take follow-ups at any time,33.4%of patients and 40.7%of families would like the follow-up in the afternoon or evening.69.7%of patients and 62.4%of families would like the follow-ups of 5 to 10 minutes. 97.0%of patients and 93.8%of families were willing to take the phone call follow-ups by physicians and 33.3%of the patients,31.2%of their families by nurses.In terms of contents,the patients and families were willing to have the medication instructions,re-examination reminder,appointments for hospitalization,dietary guidance,health counseling,exercise guidance,psychological counseling and nursing on complications and PICC.There was a high consistence on follow-up contents after discharge in cancer patients and their families(all P>0.05).Conclusion The hospitalized cancer patients and their families are willing to take telephone follow-up after discharge by the hospital staff,but most choose to have follow-ups by a doctor.The follow-up contents are multifaceted.