ObjectiveTo compare the differences in stroke subtype classification between ASCO and modified TOAST.MethodsAll 425 first-ever ischemic stroke patients were recruited and classified into different stroke subtypes according to the ASCO and modified TOAST criteria.Results Comparing ASCO grade 1 with modified TOAST,more patients were classified as atherosclerosis (60.2％ vs 57.9％ ; x2 =2.68,P=0.132) and fewer as small artery disease and cardiac disease subtype (13.4％ vs 14.8％ and 8.5％ vs 8.7％ ; x2 =1.30 and 0.00,P =0.238 and 1.000,respectively).ASCO grade 1 did not reduce the proportion of cause undetermined patients compared with modified TOAST ( 15.5％ vs 16.2％ ; x2 =0.09,P =0.795 ). Agreement was showed in every subtype categories between the two approaches.Agreement for atherosclerotic cause and small artery disease was very good ( all κ over 0.81 ),and for the cardiac disease subtype was good (κ: 0.61-0.80). Conclusion Agreement can be found in every subtype categories between ASCO and modified TOAST classification.ASCO grade 1 does not reduce the proportion of cause undetermined cases compared with modified TOAST,but the subtype categories characteristics of the two approaches should be considered in practice.

BACKGROUND:Lung invasive fungal infection is the main reason for the failure in renal transplant recipients. Caspofungin has specific anti-fungi mechanism, and can effectively prevent the candida fungus that is resistant to Fluconazol and Itraconazole. Caspofungin exerts good tolerance, without dose-or time-dependent toxicity.
OBJECTIVE:To evaluate the efficacy and safety of Caspofungin in treatment of pulmonary invasive fungal infection fol owing kidney transplantation.
METHODS:A retrospective analysis was performed in renal transplant patients who were diagnosed pulmonary invasive fungal infection in Department of Respiratory Medicine, Sanmenxia Central Hospital between January 2013 and December 2013. The patients were then treated with Caspofungin as antifungal therapy. The initial dose was 70 mg per day and then changed to 50 mg per day, via intravenous drip. After drug intervention, liver function was evaluated twice per week. The severity of liver functional impairment or emergency of new impairment indicated the adjustment of Caspofungin dose or withdrawal. The treatment was given for 10-14 days. The curative effect and adverse reaction of patients were observed.
RESULTS AND CONCLUSION:Total 12 patients were treated, and the percentage of fungi microbiology evidence was 66.7%. Among these evidenced patients, candida fungus was the dominant, accounting for 75.0%, accompanying bacterial infection was found in 58.3%, accompanying cytomegalovirus infection was found in 25.0%. The effective treatment rate was up to 91.67%(11/12), the mortality was 8.33%(1/12), and the incidence of adverse reactions was 25%. Caspofungin effectively prevented pulmonary invasive fungal infection fol owing kidney transplantation, as empirical antifungal therapy, with low incidence of adverse reactions. Therefore Caspofungin is the preferred drug against pulmonary invasive fungal infection in renal transplant recipients.

Objectives To explore the correlation between lipoprotein (a) and left main coronary artery ( LMCA) disease in patients with acute coronary syndrome (ACS). Methods A total of 264 ACS patients managed with coronary angiography (CAG) from June 2012 to December 2013 were included in the study and then they were divided into 2 groups (high-Lp(a) (> or = 300 mg/L) group (n = 93) and low-Lp(a) (< 300 mg/L)group(n = 171). The clinical data were studied and compared between the two groups. Binary unconditional logistic regression analysis was used to select the risk factors of LMCA disease. Results The percentages of multiple branch lesion (43.0% vs. 25.1%) and LMCA disease (17.2% vs. 8.2%)were higher in the high-Lp(a) group as compared with the low-Lp(a) group (P<0.05). Logistic regression analysis showed Lp(a) was independent factor of LMCA disease. Conclusions In ACS patients the elevated Lp(a) levels are associated with the severity of coronary disease and Lp(a) is an independent factor of LMCA disease.

Objective To observe the effects of exogenous basic fibroblast growth factor (bFGF) on radiation-induced apoptosis of C17.2 neural stem cells (NSCs) with γ-secretase inhibitor (DAPT) condition and explore the relationship between bFGF and Notch signal pathway.Methods The cell viability was detected by using the MTF method.After the cells attached to the flasks,different concentrations of DAPT was added in accordance with the experimental design and cultured cells for 24 h.C17.2 NSCs were subjected to irradiation exposure by linear accelerator and treated with bFGF (40 ng/ml) 5 min after the exposure.After 48 hours,the apoptosis of cells was detected by using Flow Cytometry.Results After adding in DAPT,the cells growth was inhibited and depended on the concentrations of DAPT.Compared with the control group,all groups had statistically significant differences(P＜0.05).Flow cytometry showed compared with the control group all groups had significant differences (P＜0.05).The apoptosis rate was (11.53±0.81)％ in radiation group,(7.18±0.0.94)％ in radiation+bFGF group,(9.82±0.77) ％ in DAPT group,(21.45±0.98) ％ in Radiation+DAPT group and (10.26+ 1.03) ％ in Radiation+ DAPT +bFGF group.Between Radiation + bFGF group and Radiation group,it had statistically significant difference(P＜0.05).The pairwise comparisons of DAPT group and Radiation + DAPT group which had the same DAPT concentration had statistically significant differences (P＜0.05).The pairwise comparisons of Radiation + DAPT+bFGF group and Radiation + DAPT group which had the same DAPT concentration had statistically significant difference(P＜0.05).Conclusion Fx ogenous bFGF can inhibit apoptosis of C17.2 NSCs.Notch signaling patbway inhibitor DAPT can promote apoptosis of C17.2 NSCs which are subjected to irradiation exposure by linear accelerator and bFGF can weak apoptosis.bFGF protective effect on radiation-induced neural stem cells may be related to the Notch signaling pathway.

This paper reports reaction of Bacillus lichendermis on the inhibitor vacations. The result Shows that, inhibition of the 500?g/mL of cephalosporin 1000?g/mL of penicillin, 500?g/mL of streptimycin, on the Bacillus Licheniformis,Whe the Phis 7.0. This provides a sicentific basis for using the preparation of Bacillus licheniformis.

Objective To observe the effects of Zhuyunjiaonang on morphological change of the ovary, serous hormone and the expression of P450arom in PCO model rats. To explore its target and mechanism in treating PCO model rats. Methods Using INS and HCG to establish PCO model. The PCO rat models were devided into 4 groups randomly, which included control group, model group, Zhuyunjiaonang group and mefformin group. Zhuyunjiaonang group was given 3.0 g/(kg ? d) Zhuyunjiaonang, mefformin group was given 0.75 g/(kg ? d) metformin, control group and model group were given same volume distilled water 1 mL/100 g. The effects of Zhuyunjiaonang on ovaries and ovarial follicular development were observed by light microscope. The levels of serum gonadal hormone were detected by radioimmunoassay. The expression level of P450arom was detected with immtmohistochemical staining technique. Results Zhuyunjiaonang could increase the thickness of granular cell layer and decrease the thickness of theca cell layer, decrease the level of T and LH, and increase the level of FSH and E_2 in serum. Meanwhile the expression of P450arom in ovary were increased. Conclusion Zhuyunjiaonang could indirectly or directly affect the expression of the P450arom in ovary, it could regulate the endocrine function of PCO rat and promote the mature and ovulation of follicular.

Objective To investigate the level of leptin in the serum of GDM mothers and their neonates and the variation of the leptin expression in placenta. Methods The concentrations of serum insulin and leptin in 24 GDM and 26 healthy mothers and neonates were examined by radioimmunoassay (RIA).The level of leptin mRNA in 50 placentas was examined by reverse transcription-polymerase chain reaction(RT-PCR). Results (1) The serum levels of leptin and insulin in GDM group [(18.62?7.86) ?g/L and (13.47?5.11) mIU/L] were significantly higher than those in control group [(14.21?7.59) ?g/L and (8.98?4.23) mIU/L,P 0.05) in both groups. But the leptin level in umbilical blood of the two group was positively related to the insulin level ( r=0.53,P

Objective To investigate the role of cell apoptosis in the gut mucosal barrier dysfunction in rats undergoing cardiopulmonary bypass(CPB) . Methods The rat model of CPB was set up. The rats were divided into CPB group, sham operation(SO) group and normal control group. The morphological changes of ileum mucosal tissues were observed by microscope and electron microscope at 3h, 6h, 12h and 24h after operation, respectively. The apoptotic index of gut mucosal epithelial cells was measured with TUNEL method. Results Gut mucosal morphology was normal in CPB group at 3h, 6h and 12h after operation, but gut mucosal epithelial desquamation occurred at 24h after operation. Typical apoptotic cells could be seen with electron microscope in CPB group at every time point. Apoptotic index of gut mucosal epithelial cells significantly increased in CPB group at every time point compared with SO group, and peaked at 6h after operation. Apoptotic cells were mostly located in the gut crypt. Conclusion The data suggested that the apoptosis of intestinal epithelial cells significantly increased at early stage of post-CPB in rats, which might contribute to gut mucosal barrier dysfunction.

Both microRNA and DNA methylation are the important contents of epigenetic modification.Lots of studies have confirmed that mutual regulation of microRNA and DNA methylation involve in tumorgenesis and development of cancer.The studies of their mechanisms bring some new ideas for investigating the pathogenesis of tumors,and provide a theoretical basis for the early diagnosis and treatment.

The effects of different technicological processes on the dissolubility of enteric coating aspirin tablets were studied. With testing in vitro, significant differences were found among three kinds of tablets from four factories in dissoluble feature. The length of time needed for release and that for disintegration of tablets from Factory C were 90 minutes and 5 minutes repectively, which were the shortest,and those from Factocy B, 210 minutes and 120 minutes repectively,which were the longest among three kinds of tablets. The average dissoluble amount of aspirin contained in tablets from Factory C was 74.07％;Factory D,74. 51％; Factory A, 66.6％; and Factory B, 62.51％. All the parameters determined were statistically different among three kinds of products by analysis of varianee(p