Objective: To evaluate an innovative surgical approach for submandibular gland resection with minimal invasive incision. Methods:With cutaneous incision of 30 mm in the submandibular area, six patients with chronic sialadenitis of submandibular gland were treated with this approach. Anterior facial vein and facial artery were maintained without ligation. Results:The operation time is ranged from 40 to 80 minutes. There were no facial nerve injuries. Furthermore, the surgical stigmata was minimized. Conclusion:The modified minimal incision for submandibular gland resection has less wound and better cosmesis.

Objective To discuss the method of raising the rate of cut healing by first intention with electrized copper needles and removal of the angioma.Methods Analysis of the clinical history of 68 cases of angiomatous therapy by using the method of electrized copper needles and removal, and the investigation of the connection between the therapy method and the cut healing.Results The healing rate of the cut by first intention is 100% in the total resection of tumor, each 33.33% in the partial resection of tumor and the resection under skin flap, 0% in the resection of tumor and the skin grafting on the surface of wound. When the voltage controlled at 6V is compared with others, remarkably there is the difference (P

Objective To discuss the illness mechanism and the reason of defective energy of the stomach and intestines with acute panereatitis.Method 43 cases of acute pancreatitis was studied which had the stomach and intestinal insufficiency to determine lesion degree of the stomach and intestines acording to the change of abdomen signs.Results The data found that the patient of light acute pancreatitis had light abdominal distention,cirumscribed abdominal tenderness and rebounding pain,weaken gurgling sound;most advanced acute pancreatitis had severe abdominal distention,abdominal tenderness and rebounding pain,almost no gurgling sound.Conclusion Abdominal sign could be a indicator to determine patient's condition of acute pancreatitis and a standard to diagnose acute pancreatitis.

Objective:Study on the possibility that human follicular dendritic cells(FDC) can increase HIV infection and to investigate the related mechanisms.Methods:Human FDC was isolated from tonsils and cultured with infected heterogenous pheripheral lymphocytes or cultured with infected hemogenous tonsilar T lymphocytes.HIV P24 antigen,HIV 1 env DNA and HIV 1 nef RNA were determined respectively with ELISA method,PCR or RT PCR method.Results:FDCs were cultured with infected heterogenous lymphocytes,the P24 antigen is 6 7 times higher than the control group(P

Objective To study the clinical pathological significance and relationship of placental glutathione-s-transferase(GST-?) and p53 protein expression in the tissues of human lung carcinomas. Methods The expressions of GST-? and p53 in 60 cases of lung carcinomas were detected by the LSAB immunohistochemical method. Results The positive rates of GST-? and p53 protein expression in lung carcinomas were 56 67% and 31 67% respectively. The expressions of both GST-? and p53 had obvious relationship with the differential degree of the tumors(P

Objective To investigate the change of CD8+T lymphocyte non-cytotoxic antivirus response(CNAR)in slow progressors infected by HIV.Methods Applying with density gradient and immunomagnetic beads methods to purify the CD4+T lymphocyte from the healthy person and CD8+T lymphocyte from HIV-infected individuals.The CD4+T cell was infected by HIV(SF-33)virus and cocuhured with CD8+T cell.The culture supernatant was collected and the p24 value was detected by ELISA method.Results Our study showed that the CNAR function decreased by turns of slow progressors(SP),typical progressors(TP),health control group and AIDS group.There was significant difference between groups(P<0.01).We found a significant positive correlation between the CIM+T cell ture count and the CNAR function.The virus load didn't statistically correlate with the CNAR function.Conclusion The CNAR function possibly protected the HIV-infected individuals from progression.

Objective To investigate the effects of valproic acid (VPA) on acute lung injury induced by Lipopolysaccharide in rats. Method The rat model of acute lung injury was made by intravenous injection of lipopolysaccharide (LPS). The pathological changes of lung were observed under light microscope and inflammatory cytokines in serum detected by using ELISA to judge whether the model was successfully done or not. All rats were divided into three groups as per the different intervention agents employed. Rats in control group were treated with intravenous injection of NS in dose of 5 ml/kg, rats in LPS group were exposed to LPS with dosage of 10 mg/kg and model rats in LPS + VPA group were treated with VPA in dose of 300 mg/kg. The rats were sacrificed 6 h after LPS or NS administration. The blood PaO2 ,A-aDO2 and blood lactic acid (Lac) were measured, the lungs were removed for observing the histopathological changes and determination of wet/dry lung weight (W/D) ratio and myeloperoxidase (MPO) activity as well as albumin concentration in broncho-alveolar lavage fluid (BALF) . Seurm was collected to determine the concentrations of tumor necrosis factor-a (TNF-α) and interleukin-1β( IL-1 β) by using LISA 6 h later. All data were presented in ((x)±s). One-way ANOVA was used for comparing differences between groups. Results Compared with acute lung injury group, the blood PaO2 (94. 50 ± 4.38 ) in rats of LPS + VPA group was higher, whereas A-aDO2 ( 13.50 ± 4.77 ) and blood lac( 2.13 ± 1. 02 ) in LPS + VPA group were lower. VPA significantly lowered W/D (5.33 ±0. 12) ratio and MPO activity (4.38 ±0. 42) in the lung. Albumin concentration ( 1. 260 ± 0. 039 ) in BALF, and the levels of TN F-α( 2 410 ±320 )and IL-1β( 1 220 ± 162 )in serum were lower in LPS + VPA group. The histological changes of lung injury were lessened by VPA. Conclusions Valproic acid has protective effects against lipopolysaccharide-induced acute lung injury in rats.

OBJECTIVETo explore the effect of the expanded capsule on biomechanics of the transplanted autologous costal cartilage.

METHODSTen New Zealand white rabbits aged 3 months were involved and four 15 ml tissue expanders were implanted symmetrically on the back of each rabbit. After 1 month, expanded capsules were removed (experimental group) on the left side and were reserved (control group) on the right side. Meanwhile, the 6th and 7th costal cartilage without the perichondrium were taken out and each cartilage was divided into two pieces and put under the expanded capsule symmetrically. The cartilage at the upper sides and lower sides were taken out at 4 weeks and 8 weeks later, respectively. All the cartilages were sculptured for the demand of the machine text. The tension and compression property of the cartilages were assessed by the material testing machine (Instron 5967) and the results were analyzed statistically.

RESULTS8 weeks lateral, the average ultimate tension strength was (4. 954 + 2. 8) MPa in experimental group and (2.939 + 0.842) MPa in control group. The average compressive strength was (58.74 ± 13.77) MPa in experimental group, and (47.61 ± 11.41) MPa in control group. The biomechanics property of the cartilages in the experimental group is better than the control group, and the differences were statistically significant.

CONCLUSIONSRemoving the expanded capsule is benefical to maintain the biomechanics of autologous costal cartilage.

Animals ; Autografts ; Biomechanical Phenomena ; Costal Cartilage ; physiology ; transplantation ; Humans ; Rabbits ; Ribs ; Tissue Expansion ; Tissue Expansion Devices

OBJECTIVEThis study aims to test the accuracy and precision of iWitness photogrammetry for measuring the facial tissues of mannequin head.

METHODSUnder ideal circumstances, the 3D landmark coordinates were repeatedly obtained from a mannequin head using iWitness photogrammetric system with different parameters, to examine the precision of this system. The differences between the 3D data and their true distance values of mannequin head were computed.

RESULTSOperator error of 3D system in non-zoom and zoom status were 0.20 mm and 0.09 mm, and the difference was significant (P 0.05). Image captured error of 3D system was 0.283 mm, and there was no significant difference compared with the same group of images (P>0.05). Error of 3D systen with recalibration was 0.251 mm, and the difference was not statistically significant compared with image captured error (P>0.05). Good congruence was observed between means derived from the 3D photos and direct anthropometry, with difference ranging from -0.4 mm to +0.4 mm.

CONCLUSIONThis study provides further evidence of the high reliability of iWitness photogrammetry for several craniofacial measurements, including landmarks and inter-landmark distances. The evaluated system can be recommended for the evaluation and documentation of the facial surface.

Anthropometry ; Cephalometry ; Face ; Humans ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional ; Manikins ; Photogrammetry ; Reproducibility of Results

Hydroxypropyl-chitosan with substitution degree of 0.56 is prepared by the reaction of chitosan and epoxypropane by controlling condition. The water solubility of modified hydroxypropyl-chitosan is enhanced. When suitable concentration of hydroxypropyl-chitosan water solution responds to certain concentration of sodium polyphosphate based on ion gel method, the hydroxypropyl-chitosan nano-spheres with particle size of 500-700nm are obtained. Transmission electron micrograph shows that the shape of prepared hydroxypropyl-chitosan nano-sphere is uniform.