Objective To detect the levels of Thl and Th2 type cytokines of peripheral blood CD+4 T lymphocytes in the patients with colonic cancer and to observe the shift of Th1/h2 in the patients with colonic cancer. To discuss the relationship between Th1/Th2 balance and colonic cancer progression. Methods After collecting the peripheral blood samples of research objects, the levels of IFN-γ and IL-4 were detected by flow cytometrys of Th1 Th2 cytokine marked. The data were analyzed by SPSS11.0. Results The level of IFN-γ of peripheral blood CD+4 T lymphocytes in colonic cancer patients and healthy donors were(11.7±1.86) % and (16.4±6.07)% respectively. The level of IFN-γ in malignant group was significantly lower(P ＜ 0.05). Thl/Th2 (IFN-γ/IL-4)was lower too(P ＜ 0.05). Th1/Th2 (IFN-γ/IL-4)in colonic cancer patients and healthy donors were 4.2±0.94 and 9.7±1.11 respectively. But the level of IL-4 was significantly higher than those in healthy control (P＜0.05). Conclusion Th2 dominates in peripheral blood CD+4 T lymphocytes of colonic cancer patient. Th1/Th2 disequilibrium leads to immune function hypofunction. It may be one of the reasons of colonic cancer recur, rence or colonic cancer metastasis.

Objective To observe the features of soluble MHC class I chain-related gene A (sMICA)in patients with gastric cancer of different differentiation degrees, the relationship between sMICA and the stage of gastric cancer and the relationship between sMICA and the metastasis of gastric cancer. To provide a new and useful marker in the differential diagnosis and staging of gastric cancer. Methods The peripheral blood examples of 200 gastric cancer patients and 60 health donors were collected. The serum levels of soluble MICA of peripheral blood were determined by MICA-specific enzyme-linked immunosorbent assay.Results The serum levels of sMICA in the patients with gastric cancer and health donors were (359.45 ±114.15) pg/ml and (210.07 ±31.01) pg/ml respectively. sMICA levels in the patients were significantly higher than those of the health donors (t =16.58, P <0.05). There were not significant differences among the poorly differentiated, the moderately differentiated and the well differentiated adenocarainoma(P >0.05). The serum levels of sMICA in the patients with gastric cancer steped up gradually while increasing of the TNM stages.There were significant differences between stage Ⅱ and Ⅲ, stage Ⅱand Ⅳ, stage Ⅱ and Ⅳ (P <0.05). The serum levels of sMICA in the patients with gastric cancer steped up gradually while aggravating of metastasis extent.Conclusion sMICA-release of MICA from cancer cells may reduce the expression of NKG2D. This may impair NKG2D-mediated immune surveillance of gastric cancer and leads to the tumor escaping; In the patients with gastric cancer, the serum levels of sMICA correlate with cancer stage and metastasis but show no correlation with cell differentiation.

Objective To evaluate the efficacy and toxicity of Taxol-based chemotherapy in patients with advanced gastric cancer. Methods Thirty-one patients with advanced gastric cancer were treated with two kinds of Taxol-based chemotherapy schedule. The treatment schedule were composed of paclitaxel and fluorouracil(TF) or cisplatin(TP). The patients who had had chemotherapy with fluorouracil were arranged to TP group,others to TF group. A treatment cycle lasted 21 days. The efficacy was evaluated after two cycles of the chemotherapy. Results total efficiency rate of two groups was 45.2 %; the efficiency rate of TF was 47.4 %; the rate of TP was 41.7 %; CBR was 80.6 %. The main side effect of toxicity were neutropenia and nausea as well as vomiting. Conclusion Taxol-based combination chemotherapy is a high response protocol with mild toxicity. TP schedule has a high efficiency for advanced gastric cancer.

Objective To study and evaluate the effect of developing the preventive service in venereal clinic .Methods The questionnaire survey on the selected outpatients was performed by the trained medical personnel in the venereal disease clinic of 5 medical institutions in Chongqing .The intervention services were carried out by providing healthy advices and tailor-made preven-tive packs .The healthy knowledge publicity were posted in the waiting area of the venereal clinic .The questionnaire investigation in the re-visiting patients was performed again to understand the awareness of prevention venereal disease knowledge and the condom use .Results 96 .4% of outpatients in this study accepted the AIDS-related knowledge at the first visit .84 .1% of them acquired the knowledge by consulting their doctor ,60 .9% of the patients insisted on using condom in sexual activity after accepting the interven-tion knowledge ,which was much higher than 17 .4% before intervention .Conclusion The STD clinic attenders satisfy the interven-tional preventive service in the venereal clinic ,the improvement of the venereal disease related knowledge and the adverse behaviors is obvious .

Objective To use Wilks' lambda discriminant analysis for screening of the greatest influence factors in tooth extraction correction and to establish a method for validation.Methods A total of 100 orthodontic patients were selected,aged 14 to 40 years,with average age of 23.6 years,29 males and 76 females.The conventional cephalometry analysis was conducted.Statistical analysis was carried out with using SPSS 18.0 software package.Wilks' lambda statistical method was applied to filter the largest effect on tooth extraction treatment project.Results The UIE-AB and NA/PA calculation results showed statistical differences (P＜0.05),and it set up a tooth extraction rectification regression equation for y =0.125 × NA/PA+0.351 × UIE-AB-3.429;a combination of the initial validation accuracy was 82.6％,and cross validation accuracy was 81.03％.Conclusions Incisor to AB line distance and angle of NA/PA are good tooth extraction judgement indexes.