Objective To investigate the effect of erythropoietin on hemorrhage in hip replacement surgery .Methods 48 cases undergoing elective hip replacement surgery in our hospital From June 2014 to October 2016 were selected and devided into control group and experimental group according to the conditional similarity, 24 cases in each group, then the application of erythropoietin in hip replacement surgery was explored.For the experimental group, erythropoietin was injected subcutaneously at three, five and seven days before operation, the dose was 250 U/(kg· times).The control group received 0.9%sodium chloride injection of the same volume of saline, and the other treatments were the same as in the experimental group.Preoperative situation, hemoglobin (Hb) levels, blood pressure (diastolic pressure and systolic blood pressure) at one day before surgey and three, seven and 14 days after surgey were compared and analyzed.Results There were no significant difference in body weight, hemoglobin (Hb), and age between the two groups before operation.The levels of Hb in the experimental group and the control group at 3 and 7 days after operation were compared, the difference was statistically significant (P<0.05), 14 days after the surgey, Hb returned to normal in the experimental group, while the Hb level in the control group was still significantly lower than that in the control group before surgey, the difference was statistically significant (P<0.05).There was no significant difference in diastolic blood pressure and systolic blood pressure between the two groups.Conclusion Preoperative erythropoietin mobilization in patients with hip replacement surgery can reduce the amount of bleeding during and after operation , and the adverse reactions are not obvious.

Objective To investigate the clinical curative effect and the influence on knee joint function of knee osteoarthritis treating with sodium hyaluronate combined with compound salvia injection.Methods 78 patients with osteoarthritis treated in our hospital from June 2015 to August 2016 were randomly divided into three groups: sodium hyaluronate group, compound salvia injection group and sodium hyaluronate plus compound salvia injection combination therapy group, 26 cases each group.The levels of serum inflammatory cytokines (IL-6,TNF-αand IL-1β) were measured by ELISA before and after treatment in the three groups, and the improvement of knee joint function and the change of pain score before and after treatment were analyzed as well.Results The total effective rate of sodium hyaluronate combined with compound salvia injection was significantly higher than that of sodium hyaluronate and compound salvia injection alone ( P <0.05 ); after treatment, patients VAS pain score was significantly decreased ( P <0.05), and the VAS pain scores in the combination group were significantly lower than those of the group treated with sodium hyaluronate and the compound salvia injection (P<0.05).The levels of IL-6, TNF-αand IL-1βin the three groups were significantly lower than those before treatment (P<0.05),and the inflammatory factors in the combination group were significantly lower than those in the alone treatment group (P <0.05). Conclusion Sodium hyaluronate combined with compound salvia injection has good clinical curative effect on knee osteoarthritis,improving the function of knee joint, reducing pain and so on.

Objective: To investigate the role of miR-142-3p in alleviation of house dust mite induced allergic airway inflammation among children, so as to provide insights into unraveling the pathogenesis of allergic airway inflammation. Methods: Serum samples were collected from 15 patients with house dust mite induced allergic asthma and 15 healthy children in Jiangnan University Medical Center from September to November 2022, and serum miR-142-3p expression was quantified using a fluorescent quantitative real time PCR (qPCR) assay. The levels of interleukin 6 (IL 6) and tumor necrosis factor α (TNF α) were measured in the cell culture supernatant using enzyme linked immunosorbent assay (ELISA), and the expression of high mobility group box 1 (HMGB1) was detected at transcriptional and translational lvels using qPCR and Western blotting assays. The negative regulation of the HMGB1 gene by miR 142 3p was identified using a dual luciferase gene reporter assay, and the expression of downstream regulatory proteins was determined in human normal lung epithelial cells (BEAS 2B) cells transfected with miR 142 3p using Western blotting. In addition, female C57BL/6 mice at ages of 6-8 weeks were randomly assigned to the phosphate buffer saline (PBS) group, house dust mite sensitized airway inflammation group and house dust mite sensitized airway inflammation + miR 142 3p intervention group. Mouse airway inflammation was evaluated using hematoxylin eosin staining, and the expression of inflammatory cells and inflammatory cytokines were detected in mouse bronchoalveolar lavage fluid (BALF) using Giemsa staining and ELISA. Results: Lower serum miR-142-3p expression was quantified among children with house dust mite induced allergic asthma than among healthy controls (1.33±0.21 vs. 4.74±0.62, t=5.22, P <0.05). Stimulation with dermatophagoides farinae extract (DFE) resulted in a reduction in miR-142-3p expression in BEAS-2B cells (0.82±0.25), while transfection with miR-142-3p mimics resulted in a rise in miR-142-3p expression in BEAS-2B cells (0.55±0.14)( t=3.31, 3.94, P <0.05). Pre treatment with miR-142-3p reduced the expression of IL 6(2.25±0.46)and TNF α(6.58±1.95) ( t=4.86, 3.38, P <0.05) in BEAS 2B cells stimulated with DFE, and treatment with miR-142-3p mimics resulted in a reduction in TLR4 and NF-κB expression in BEAS-2B cells via negative regulation of the HMGB1 expression. In addition, treatment with miR-142-3p was found to alleviate inflammatory cell infiltration in lung tissues of house dust mite sensitized mice, and results in a reduction in interleukin 4 (IL-4)[(107.60±10.43)pg/mL], interleukin 5 (IL 5)[(95.78±13.14)pg/mL] and HMGB1[(2.52±0.87)pg/mL] expression in BALF ( t=10.32, 7.29, 2.90, P <0.05). Conclusion miR-142-3p alleviates house dust mite induced allergic airway inflammation among children via negative regulation of the HMGB1/TLR4/NF-κB pathway.

The dose-related adverse effects of MDM2‒P53 inhibitors have caused significant concern in the development of clinical safe anticancer agents. Herein we report an unprecedented homo-PROTAC strategy for more effective disruption of MDM2‒P53 interaction. The design concept is inspired by the capacity of sub-stoichiometric catalytic PROTACs enabling to degrade an unwanted protein and the dual functions of MDM2 as an E3 ubiquitin ligase and a binding protein with tumor suppressor P53. The new homo-PROTACs are designed to induce self-degradation of MDM2. The results of the investigation have shown that PROTAC