Objective To evaluate the efficacy of transcatheter arterial chetnoembolization (TACE) in adjuvant with ther - motherapy in the treatment of primary hepatic carcinomas (PHCs) ,and to obsorre the changes of T - lymphocyte subsets and naturalkiller(NK) cells before and after therapy and the clinical significance. Methods Forty patients with advanced PHCs were randomly divided into two groups (the study group and the control group) ,with 20 cases in each. The patients in the study group were treated by TACE in adjuvant with thermotherapy, and the patients in the control group were treated by TACE alone. The changes of T - lymphocyte subsets and NK cells were examined by im - munofluorescence staining in 40 cases of PHC before and after TACE in adjuvant with ther - motherapy and were compared with those of 20 cases who received conventional interventional chemotherapy (control group). Results The short -term remission rate of the study group and control group were 65% and 40% , respectively (P

In liver transplantation,hepatic sinusoidal obstruction syndrome (HSOS) is rare,which results in misdiagnosis and mistreatment.The prognosis of HSOS after liver transplantation is related to the implementation of early specific treatment.No systematic review has been reported on HSOS after liver transplantation.To raise awareness of HSOS after liver transplantation,this review summarizes the aetiology,pathology,clinical manifestations,laboratory findings,imaging features,diagnosis,differential diagnosis,treatment and prognosis of the condition.

OBJECTIVE:To explore the role of clinical pharmacists participating in the prevention and treatment of sotalol-in-duced QT prolongation in aged patient with heart failure. METHODS:Clinical pharmacists participated in the treatment for a aged patient with heart failure,and assisted physicians to identify and assess the risk of sotalol-induced QT prolongation. According to the clinical symptoms and laboratory indexes,it was suggested to continue to use sotalol for antiarrhythmic,adjust the hepatoprotec-tive drug,given pharmaceutical care of ECG,electrolyte level monitoring,drug education and so on. RESULTS:Physicians adopt-ed the suggestions of clinical pharmacists to relive the illness without malignant arrhythmia and discharged after 11 d. CONCLU-SIONS:The patient with heart failure should be dominated by improving cardiac function and maintaining hemodynamic stability;combined with related guidelines and documentation,clinical pharmacists can assist physicians to identify the drug that induced QT prolongation,asses the risk of torsades de pointes ventricular tachycardia and other bad consequences,develop and optimize the regimen and strengthen pharmaceutical care to ensure the safe and effective treatment.

Objective To investigate expression and role of tissue factor pathway inhibitor -2(TFPI -2)in normal cervix,cervical intraepithelial neoplasia(CIN)and cervical squamous cell carcinoma.Methods From Janu-ary 2011 to December 2012,114 patients with cervical resection in our hospital were selected,among them,42 patients with cervical squamous cell carcinoma,27 cases with CIN,45 cases of normal cervix.Immunohistochemical method was used to detect the expression of TFPI -2 protein in cervical tissues.Results The expression of TFPI -2 in cer-vical cancer tissues was significantly lower than that in other cervical tissues,the difference was statistically significant (cervical cancer tissues vs.normal cervical tissues χ2 =59.670,P <0.05;cervical cancer tissues vs.CINⅠ χ2 =52.170,P <0.05;cervical cancer tissues vs.CINⅡ/Ⅲ χ2 =32.759,P <0.05).The expression of CIN Ⅱ/Ⅲ TFPI-2 was significantly lower than that of normal cervical tissues and CIN Ⅰ,the difference was statistically significant (χ2 =126.78,P <0.05).The expression level of TFPI -2 in normal cervix tissues and CIN Ⅰ had no statistically significant difference(χ2 =4.903,P >0.05).There was no significant difference in TFPI -2 expression in cervical cancer tissues with different differentiation levels(χ2 =4.633,P >0.05).Figo Ⅰcervical cancer TFPI -2 expression was significantly higher than Figo Ⅱ cervical cancer,the difference was statistically significant(χ2 =6.509,P <0.05).The expression of TFPI -2 in cervical cancer tissues without lymph node metastasis was significantly higher than that in cervical cancer tissues with lymph node metastasis,the difference was statistically significant (χ2 =10.916,P <0.05).Conclusion The reduction of TFPI -2 expression may be involved in the development of cervi-cal cancer,and the expression of TFPI -2 is associated with the prognosis of cervical cancer.

Objective To investigate the relationship between serum neuron-specific enolase (NSE) level and the severity of brain injury in patients with acute ischemic stroke. Methods A total of 100 patients with acute ischemic stroke, classified as total anterior circulation infarct (TACI), partial anterior circulation infarct (PACI), posterior circalation infarct (POCI) and lacunar infarct (LACI) were included in this study. The serum levels of NSE were detected and cerebral infarc-tion volumes were measured using 32-bit OsiriX imaging software. The correlation between serum level of NSE, cerebral in-farction volume and subtypes of ischemic stroke was analyzed. Results Patients were divided into NSE≤11.46μg/L group and NSE>11.46μg/L group. The scores of NIHSS[6.0(4.0, 10.0) vs 4.0(2.0, 6.0)]were higher, the infarction volumes[4.3 (1.3, 15.9) cm3 vs 0.8(0.4,4.3) cm3]were larger in NSE>11.46μg/L group (P<0.05). The serum NSE level showed statistical-ly significant difference between TACI and other groups (P<0.01). The increased serum NSE level was correlated with in-creased infarction volume (rs=0.417,P<0.01). There was a linear regression between serum NSE level and infarction vol-ume. Its linear regression equation was =2.438+0.100 X (X:infarction volume;Y:serum NSE level). Conclusion The se-rum NSE level may be helpful for the prediction of the severity of brain injured in patients with acute ischemic stroke.

Hepaticbililary surgery interventional therapy is a technique which applied to hepaticbilliary diseases for diagnosis and treatment presently.With the development and widespread of interventional therapy technology,there are increasing demands for interventional doctors.But the interventional therapy with invasive and radioactive makes itself impossible for interns to have much chance of actual practice,which becomes the "bottleneck"of the technology development of teaching.Computer-assisted instruction has become an important approach in medical education.It is possible that multimedia courseware can break through the "bottleneck".So we decide to design and make a kind of multimedia courseware adapted to hepaticbililary surgery intentional therapy to improve quality and efficiency of teaching

Biological monitoring is one of methods of water environment monitoring. By using the reaction of biological individual,population and community to environmental pollution,biomonitoring can expound the pollution state,provide monitoring data for the environmental quality assessment. The sensitivity,bio-concentration,long-term and comprehensive characteristics are advantages of biomonitoring. This paper summarized the methods of the biomonitoring used in practise,including biotic index,toxicity test,polyurethane foam unit,simultaneous determination,ecotoxicogenomics and involved single cell algae,protozoana,benthic organisms,amphibian. The shortages of the monitoring method also exist,for example,the regional limitation.

Non-surgical therapy is important to liver cancer treatment. Actinotheraphy and chemotherapy, because of their less specific for cancer tissue and serious side effect, badly affect the patients' life qualities, sometimes even endanger the patients lives. Hepatocellular carcinoma specific antibodies have been the focus of liver cancer biotherapy. After 20 years of study since 1975, it has been demonstrated that antibodies derived from mouse could not efficiently stimulate the functions of human reactor, but could induce human anti-mouse antibody responses which could bring about serious side effects. The application of DNA recombination technique provides a good method for resolving the immune selection problem. The techniques of humanoriginated antibodies and phage displaying will lead to development of innovative strategies to manage liver cancer. In our country, the antibody products aimed at cancer all originated from mouse while the study of human antibodies is still at its primary stage. Non-human antibodies has been forbidden in clinical trails by FDA in America since 2001. It can be speculated that human antibody products will domminate the future market rapidly.

Twenty-five compounds of novel quinoxaline-based scaffold with antiplatelet activity were designed and synthesized on the basis of previous quinoxaline analogues, and the structures were confirmed by ¹H NMR, ¹³C NMR, and MS. The antiplatelet activity was evaluated, structure-activity relationship (SAR) study was summarized and the selectivity of PAR4 was confirmed by calcium mobilization assays. It was indicated that compound 14a, 14g, 13i, 13p showed moderate activity against PAR4, especially, the activity of compound 14g (IC₅₀ = 0.26 μmol·L^-1) was 6.7 times than the lead compound A (IC₅₀ = 1.73 μmol·L^-1). Therefore, 2,3-dihydro-[1,4]dioxino[2,3-g]quinoxaline and [1,3]dioxolo[4,5-g]quinoxaline derivatives are promising compounds for the discovery of novel antiplatelet agents. It is worthy of further research to develop highly effective and selective PAR4 antagonists.

Constructing prokaryotic expression vector pKY-RMBAY by gene recombination and research its optimizing productive conditions.By PCR technology synthesizing the gene of the RMBAY with preference codon of E.coli and the RMBAY gene was inserted into high efficiency expression vector pKYB-MCS.Expressed fusion proteins in E.coli ER2566 were purified with Chitin-Beads column.Fusion proteins binding on Chitin-Beads was cut on N-terminus of intein due to the induction of ?-mercaptoethanol and the target peptide RMBAY was released.The RMBAY was identified by mass spectrum.Experiment results showed RMBAY can be high efficiently expressed in E.coli ER2566,with optimizing productive conditions the yield of the RMBAY may be 6.7mg/L fermentation product and its purity is greater than 98%.The molecular weight of RMBAY is 3.887 kDa by mass spectrum and that accords with its theory value.