Burnout, Professional ; Humans

Humans ; Occupational Diseases ; prevention & control ; Occupational Health ; Stress, Psychological ; prevention & control

Cardiovascular Diseases ; etiology ; Humans ; Psychology, Industrial

Hazardous Substances ; Risk Assessment ; methods ; Workplace

Carbon Disulfide ; analysis ; Occupational Exposure ; prevention & control ; Threshold Limit Values ; Workplace

Humans ; Musculoskeletal Diseases ; epidemiology ; prevention & control ; Occupational Diseases ; epidemiology ; prevention & control

Adult ; Female ; Humans ; Logistic Models ; Male ; Mental Health ; statistics & numerical data ; Transients and Migrants ; psychology ; Young Adult

AlM:To investigate the effects of pirfenidone ( PFD) on the proliferation and transfomring growth factor-β1 ( TGF-β1 ) expression in vitro culture rat corneal stromal cells.METHODS: Corneal stromal cells from 8 to 10wk SD rats were isolated, cultured and treated with different concentrations of PFD 0mg/mL (control group), 0. 15mg/mL (experimental group▏), 0. 3mg/mL (experimental group‖), 1mg/mL (experimental group Ⅲ) for 48h. CCK-8 assay was performed to assess cell proliferation, while immunocytochemistry and Western Blot were used to detect the expression of ki-67 and TGF-β1 expression, respectively. RESULTS: Compared with control group, PFD significantly inhibited the proliferation in a dose -dependent manner ( all P < 0. 05 ), so was protein expression of ki-67. PFD significantly down-regulated the expression of TGF-β1 in a dose-dependent manner (P<0. 05).CONCLUSlON: Pirfenidone can significantly inhibit the proliferation of rat corneal stromal cell by down regulating TGF-β1 expression, therefore, it has potential prospect in lightening the corneal wound healing reaction.

OBJECTIVETo find out whether tumor necrosis factor (TNF)-alpha-308 and TNF-alpha-238 locus (G-->A) mutation within the TNF-alpha gene promoter region is associated with susceptibility to occupational stress.

METHODS169 pipelining workers were investigated using the occupational stress measuring instrument. The subjects were divided into high and low strain groups according to the job strain score of the questionnaire. By means of polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) techniques, TNF-alpha gene polymorphisms of all subjects were analyzed.

RESULTSThere were 34 workers with genotype -308G/G and 4 with -308G/A genotype in the low strain group while in the high strain group, there were 117 workers with genotype -308G/G and 10 with -308G/A genotype and 1 with -308A/A. No significant difference in -308 genotype was found between the two groups (chi2 = 0.0001, P > 0.05). There were 30 workers with genotype -238G/G and 7 with -238G/A genotype and 1 with -238A/A in the low strain group while there were 118 workers with genotype -238G/G and 10 with -238G/A genotype in high strain group. The frequencies of TNF-alpha-238A in the low strain groups were significantly higher than that in high strain groups (chi2 = 5.45, P < 0.05).

CONCLUSIONTNF-alpha promoter G238A polymorphism is associated with occupational stress. There is no evident relationship between TNF-alpha promoter G308A polymorphism and susceptibility to occupational stress.

Female ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Humans ; Male ; Occupational Diseases ; psychology ; Polymorphism, Genetic ; Promoter Regions, Genetic ; Stress, Psychological ; genetics ; Surveys and Questionnaires ; Tumor Necrosis Factor-alpha ; genetics

OBJECTIVETo explore the relationship between social support and occupational stress.

METHODSA cross-sectional survey involving 654 career people from different industries was conducted. Social support, the occupational stressors, personalities, and occupational strain were measured by using Occupational Stress Instrument.

RESULTSThe people from different occupations got different level of social support. Social support scores were significantly positively related to interpersonal relation, responsibility for people, responsibility for thing, advancement and participation, task identity, training adequacy, challenge, autonomy, job satisfaction, mental health, affective ambulance, positive emotion, self-esteem and coping strategy (P<0.05 or P<0.01), negatively to careers, role conflict, role ambiguity, job future ambiguity, negative emotion, depression symptoms, daily life stress and work locus of control scores (P<0.05 or P<0.01). The scores of interpersonal relation, responsibility for people, responsibility for thing, advancement and participation, task identity, training adequacy, challenge, autonomy, job satisfaction, mental health, affective ambulance, positive emotion, self-esteem and coping strategy with sufficient social support were significantly higher than those of deficient social support (P<0.05 or P<0.01). The scores of role conflict, role ambiguity, job future ambiguity, negative emotion, depression symptoms, daily life stress and work locus of control were higher than those of the latter (P<0.05 or P<0.01). Stepwise regression analysis showed that social support was the predictor of depression (R(2)>0.05).

CONCLUSIONSocial support affects occupational stress largely and plays an important role in maintaining mental health of career people. The people from different occupations get different level of social support.

Adult ; Burnout, Professional ; Cross-Sectional Studies ; Female ; Humans ; Male ; Social Support ; Young Adult