1.Changes of cytokine levels before and after anti-tuberculosis treatment
GUO Shan-shan ; LI Shu-tao ; YANG He ; KOU Bin
China Tropical Medicine 2022;22(09):806-
Abstract: Objective To analyze the changes of cytokine levels in patients with sensitive pulmonary tuberculosis and drug-resistant pulmonary tuberculosis before and after anti-tuberculosis treatment. Methods The clinical data of 213 pulmonary tuberculosis patients admitted to the Eighth Affiliated Hospital of Xinjiang Medical University from August 2019 to October 2020 were collected, and divided into a sensitive pulmonary tuberculosis group and a drug-resistant pulmonary tuberculosis group, of which 141 were in the sensitive group and 72 in the drug-resistant group , and the pro-inflammatory factors tumor necrosis factor-α (TNF-α), IL-6, IL-17, interferon-γ (IFN-γ) and anti-inflammatory factors IL-10 and IL-4 in plasma before treatment, 3 months and 6 months after treatment were compared. Results Compared with the healthy group, the levels of plasma IL-6 in the sensitive group and the resistant group were significantly increased, difference was statistically significant (P<0.01). The plasma levels of IFN-γ and IL-17 in the sensitive group were increased, and the difference was statistically significant (P<0.05). Compared with before treatment, the levels of IL-6 and IL-4 in the plasma of the sensitive group after 6 months of treatment were lower than those after 3 months of treatment, and the difference was statistically significant (P<0.05), and IFN-γ was significantly decreased (P<0.001). After 6 months of treatment, the IL-10 content was lower than that before treatment, and the difference was statistically significant (P<0.05), and the IFN-γ decreased significantly (P<0.000 1). The levels of IFN-γ at 3 months were higher than those in the resistant group, and the difference was statistically significant (P<0.01). After 6 months of treatment, there was no significant difference in the content of IFN-γ between the sensitive group and the resistant group. The effective rate of CT absorption was 70% in the sensitive group and only 43.33% in the resistant group at 3 months of treatment, and there was no significant difference in the effective rate between the two groups after 6 months of treatment. Conclusions Detection of pro-inflammatory cytokines and anti-inflammatory cytokines in plasma can provide reference for host-directed therapy of pulmonary tuberculosis. Understanding this important feature of cytokines on pathogen-host interactions can provide new insights into the study of virulence mechanisms and may provide new approaches for immune intervention in pulmonary tuberculosis.
2.The study of laparoscopic-guided radiofrequency ablation of prostate cancer
Bin GUO ; Wenqi WU ; Xiaogang LU ; Dongliang ZHONG ; Chichang SHAN
Chinese Journal of Postgraduates of Medicine 2011;34(29):14-16
Objective To evaluate the feasibility and efficiency of laparoscopic-guided radiofrequency ablation on advanced prostate cancer.Methods From March 2003 to December 2008,a total of 6 previous prostate cancer patients who had been diagnosed with pathological results were treated by laparoscopic-guided radiofrequency ablation.All patients underwent pre-and post-operative IPSS,serum PSA,MRI and normal blood biochemistry examination.The treatment outcome,surgery-related complications were also recorded.Results All operations were successfully completed,no serious intra-and post-operative complications happened.Although there was no significant difference of IPSS between pre-operative [ ( 19.05 + 4.28 ) scores ] and 1 month after operation [ ( 19.87 + 5.72) scores ],but there were significantly decreased in 3 months [ (9.45 ± 2.03 ) scores ] and 6 months [ (6.18 + 1.79) scores ] after operation (P <0.05).Also being followed up to 6 months after operation,the serum PSA was significantly decreased compared with the pre-operative value [from(24.80 ± 14.56) μ g/L reduced to( 13.79 ± 7.76) μ g/L](P<0.05).Conclusion Laparoscopic-guided radiofrequency ablation on advanced prostate cancer is safe and feasible,and can be used as an effective treatment in selective cases.
3.Development and Application of Portable Bracket of Lower Limb in Operation of Tibia Interlocking Intramedullary Nail
Zhimin GUO ; Kejian LIAN ; Zhenqi DING ; Shan LIN ; Bin LIN ; Zemin ZHUANG ; Linxin GUO
Chinese Medical Equipment Journal 2003;0(11):-
Objective To develop and apply portable bracket of lower limb in operation of tibia interlocking intramedullary nail. Methods Portable bracket of lower limb is composed of two fir panels and support structure. The level of support and the knee flexion is controlled with it. It is used in preoperative high-pressure disinfection, and then trouble limb is placed on the bracket to complete operation of tibia interlocking intramedullary nail. Results The applications save labor, reduce iatrogenic injury, make for union of fracture and avoid radiation damage of staff. No one is used to lift up leg in operation procedure and X-ray examination. Conclusion Potable bracket of lower limb has advantages in simple production, low cost, labor-saving, minimally invasive surgery wound and so on.
4.Portal vein thrombosis after partial splenic embolization
Mingyue CAI ; Xiaochun MENG ; Junwei CHEN ; Wensou HUANG ; Bin ZHOU ; Yongjian GUO ; Kangshun ZHU ; Hong SHAN
Chinese Journal of General Surgery 2011;26(12):1002-1004
Objective To investigate the clinical outcome and treatment of portal vein thrombosis (PVT) following partial splenic embolization (PSE).Methods From April 2006 to April 2010,105patients with hypersplenism caused by cirrhotic portal hypertension were treated with PSE.Contrastenhanced abdominal computed tomography or magnetic resonance imaging was performed routinely in 60patients before PSE and 1 -3 months after PSE.PVT was detected in 10 patients on images after the procedures.After PVT was diagnosed,4 patients received anticoagulant therapy immediately,and the other 6 patients did not receive therapy.Clinical data of these 10 PVT patients were analyzed retrospectively.Results 3 of 4 patients who received anticoagulant therapy had complete or partial resolution of the thrombus,and one developed mild ascites without thrombosis progression.Of the 6 patients who did not receive anticoagulant therapy,follow-up studies (6- 48 months,mean 16.9 months) demonstrated partial clot calcification in one,thrombosis progression in 5.Among those 5 patients with thrombosis progression,two experienced hematemesis due to variceal rupture and underwent transjugular intrahepatic portosystemic shunt,2 developed cavernous transformation,extensive collateral circulation,ascites and variceal progression,and one had variceal progression with melena during the follow-up period.Conclusions PVT is a severe complication of PSE.Early diagnosis and prompt anticoagulant therapy is effective in preventing PVT.
5.Treatment of tibial plateau fracture with minimal invasion spinal surgery in 36 cases
Jun-Wu HUANG ; Bin LI ; Xiao-Shan GUO ; Ben FAN ; Lei PENG ; Yong-Long CHI
Chinese Journal of Trauma 1993;0(06):-
Objective To investigate the clinical results of minimal invasion spinal surgery (MISS) in treating tibial plateau fractures to provide best procedure for such treatment.Methods The study involved 36 cases with tibial plateau fractures resulted from traffic injury,fall-from-height injury and strain injury including 24 males and 12 females at age of 19-65 years (average 42.6 years).According to Schatzker classification,there were seven cases of typeⅠ,five typeⅡ,five typeⅢ,five type IV,sev- en typeⅤand seven typeⅥ.All cases were treated by different methods of MISS.Results Follow up for 8-24 months in 35 cases showed fracture healing within 3-4 months,without severe complications such as poor wound healing,infection or osteofascial compartment syndrome.Meanwhile,no traumatic knee osteoarthritis,inversion or eversion of the knee were found.The mean range of knee motion was 94?,with fine knee function in 32 cases (91.4%) according to Merchant score.Conclusions Treatment of tibi- al plateau fractures should adopt limit incision,indirect reduction and biofixation to take place widespread exposure and rigid plate fixation.MISS is safe and effective for tibial plateau fractures.
6.Mechanism of emodin for anti-fibrosis of liver.
Yu-tao ZHAN ; Bin LIU ; Ding-guo LI ; Chun-shan BI
Chinese Journal of Hepatology 2004;12(4):245-246
Animals
;
Cell Division
;
drug effects
;
Emodin
;
pharmacology
;
therapeutic use
;
Liver
;
drug effects
;
pathology
;
Liver Cirrhosis, Experimental
;
drug therapy
;
metabolism
;
pathology
;
Male
;
Rats
;
Rats, Sprague-Dawley
;
Transforming Growth Factor beta
;
analysis
;
Transforming Growth Factor beta1
7.Construction of recombinant adenovirus vector carrying human miR-133a and its expression in vitro
Bin ZHANG ; Zhixin SHAN ; Qiuxiong LIN ; Zhiling ZHOU ; Chunyu DENG ; Ailin GUO ; Yongheng FU ; Honghong TAN ; Xiyong YU
Chinese Journal of Pathophysiology 2000;0(07):-
AIM:To construct recombinant adenovirus vector carrying human miR-133a and study its expression in human mesenchymal stem cells(hMSCs).METHODS:The PCR product containing miR-133a was amplified from human genomic DNA and inserted into the adenoviral shuttle vector pAdTrack-CMV.Then the recombinant shuttle plasmid linearized by pmeⅠwas cotransformed into competent E.coli.BJ5183 with the adenoviral backbone plasmid pAdEasy-1 to generate the recombinant adenovirus vector rAd-mir-133a.rAd-mir-133a was then packaged and amplified in human embryonic kidney 293(HEK293) cells.The purified rAd-miR-133a was used to infect the hMSCs and the expression of miR-133a was detected by non-quantitative RT-PCR and real-time PCR.RESULTS:The recombinant adenovirus shuttle vector pAdTrack-CMV-miR-133a was constructed and verified by restriction endonuclease analysis and DNA sequence analysis.rAd-miR-133a was successfully packaged and amplified in HEK293 cells.The transcriptions of primary miR-133a and mature miR-133a were over-expressed in the hMSCs infected with rAd-miR-133a.CONCLUSION:The recombinant adenovirus vector carrying human miR-133a is successfully constructed,which lay a foundation for miR-133a function study.
8.A cross sectional study of chronic pain relief after bekam (traditional malay “cupping”) therapy
Kean Ghee Lim ; Shu Whey Chuah ; Michelle Ee Shan Too ; Zheng Guo Wong ; Ashwin Murugesan ; Syed Ameer Bin Syed Azman
International e-Journal of Science, Medicine and Education 2015;9(2):32-36
Introduction: Bekam, an Islamic variant of cupping,
is an ancient form of traditional medicine still practised
today in Malaysia. There are published findings
indicating that cupping benefits patients with low back
pain, other musculoskeletal pain and even pain from
cancer, herpes zoster and trigeminal neuralgia when
pain is measured on an analogue scale. We proposed to
investigate whether in addition to pain improvement on
an analogue scale we could show if pain relief might be
demonstrated in terms of reduction of analgesic use.
Methods: We carried out a retrospective cross sectional
study on subjects who had been for outpatient clinic
treatment with chronic pain of at least one month and
who completed at least two bekam therapy sessions.
In addition to documenting a pain score before and after
therapy we documented their analgesic consumption.
Results: A total of 77 respondents, with overlapping
symptoms of headache, backache and joint pains were
included. The mean pain score before bekam therapy
was 6.74±1.78, and was 2.66±1.64 after two sessions
of therapy. Twenty eight respondents completed
six sessions of bekam therapy and had a mean pain
score of 2.25±1.32 after. Thirty-four patients consumed
analgesic medication before starting bekam therapy and
only twelve did so after. The consumption of analgesics
was significantly lower after bekam therapy.
Conclusions: Bekam therapy appears to help patients
experience less pain and reduce the amount of analgesic
medication they consume. Nevertheless only a
randomised prospective study will eliminate the biases
a retrospective study is encumbered with and we believe
would be worth doing.
Medicine, Traditional
;
Chronic Pain
9.Determination of levodropropizine and its pharmacokinetics in human plasma using LC/MS/MS.
Li-mei ZHAO ; Li ZHAO ; Ya-xin SUN ; Feng QIU ; Shan-bin GUO
Acta Pharmaceutica Sinica 2004;39(12):993-996
AIMTo develop a rapid and sensitive LC/MS/MS method for the analysis of levodropropizine in plasma and study the pharmacokinetics of levodropropizine in healthy Chinese volunteers.
METHODSLevodropropizine and zolmitriptan (internal standard, IS) were extracted from plasma samples and chromatographed on a C18 column and detected using a tandem mass spectrometer with a TurboIon Spray ionization interface. Quantitation was performed using multiple reaction monitoring (MRM) of the transitions of the m/z 237 --> m/z 120 for levodropropizine and m/z 288 --> m/z 58 for the IS.
RESULTSThe limit of quantification of the method for levodropropizine was 0.25 microg x L(-1). The assay was linear over the concentration range from 0.25 to 500.0 microg x L(-1) and intra- and inter-day precision over this range were < 11.4% with good accuracy.
CONCLUSIONThe method is shown to be accurate, and suitable for clinical pharmacokinetic study of levodropropizine.
Administration, Oral ; Antitussive Agents ; blood ; pharmacokinetics ; Area Under Curve ; Chromatography, Liquid ; Humans ; Male ; Propylene Glycols ; administration & dosage ; blood ; pharmacokinetics ; Spectrometry, Mass, Electrospray Ionization
10.Effect of plasma fibrinogen level-based defibrase therapy in patients with acute cerebral infarction.
Xiao-bin CAI ; Zhi-shan ZHU ; Ming-zhi ZHANG ; Jin GUO ; Hui-lan WANG
Journal of Southern Medical University 2009;29(10):2124-2127
OBJECTIVETo evaluate the therapeutic effect of individualized defibrase therapy according to the level of plasma fibrinogen (FIB) in patients with acute cerebral infarction (ACI).
METHODSSixty patients with ACI (within 72 h after onset) were randomly divided into defibrase group (n=30) and control group (n=30). The patients in defibrase group received intravenous defibrase infusion at different first doses (15, 10, and 5 U) according to plasma FIB level (>4 g/L, 2-4 g/L, and 1.3-2 g/L) before treatment. Plasma FIB was measured every 12 h after the first dose of defibrase, and when plasma FIB was over 1.3 g/L, intravenous infusion of 5 U defibrase was given to maintain plasma FIB within the range of 0.70-1.13 g/L over a period of 7 days. The plasma prothrombin time (PT), activated partial thromboplastin time (APTT) and FIB before and after the 7-day treatment were measured, and the scores of Chinese stroke scale (CSS) after 14 days of treatment and Activity of Daily Living (ADL) after 3 months were recorded.
RESULTSAfter 7 days of treatment, plasma PT and APTT were significantly prolonged lengthened and plasma FIB was lowered in defibrase group. The scores of CSS improved in defibrase group after 14 days of treatment, showing significant difference from those of the control group. The clinical effective rate was 80% in defibrase group, significantly higher than that in the control group (50%). The scores of ADL after 3 months were similar between the 2 groups, but the percentage of independent living and mild dependency was significantly higher in defibrase group (93.3% vs 70.0%). No intracerebral and extracerebral hemorrhage occurred in defibrase group the during treatment, no did death occur after 3 months of treatment.
CONCLUSIONDefibrase therapy based on plasma FIB level can rapidly and effectively lower plasma FIB, reduce neurological impairment and improve the quality of life in patients with ACI.
Acute Disease ; Adult ; Aged ; Aged, 80 and over ; Batroxobin ; therapeutic use ; Cerebral Infarction ; drug therapy ; Female ; Fibrinogen ; metabolism ; Fibrinolytic Agents ; therapeutic use ; Humans ; Male ; Middle Aged