OBJECTIVE: To establish an HPLC method for the determination of chloramphenicol content in chloramphenicol vaginal soft capsule.METHODS: The sample was separated on Kromasil C18 column with 0.1% sodium heptanesulfonate solution-acetonitrile(77∶23) as mobile phase at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 272 nm;The sample size was 10 ?L and the column temperature was maintained at 30 ℃.RESULTS: The linear range of chloramphenicol was 0.079～0.113 mg?mL-1(r=0.999 6) and its average recovery rate was 99.38%(RSD=0.42%).CONCLUSION: The method was proved to be specific,sensitive,accurate in results and suitable for the determination of chloramphenicol content in chloramphenicol vaginal soft capsule.

Objective To explore the clinical significance of gastric hyperplastic polyp pits under magnifying endoscopy. Methods Summary confirmed by magnifying endoscopy and pathological examination gastric hyperplastic po]yp's performance of magnifying endoscopy,Hp positive and the results of pathology during February 2004 to De-cember 2006. Results 55 patients found 116 gastric polyp,41.4% (48/116) with type A,26.7% (31/116) with type B,19. 8% (23/116) with type C,8. 6% (10/116) with type D,2. 6% (3/116) with type E,one case gastric pits blurred(type F) 0. 9% (1/116). 4 cases with type E and type F found moderate-severe atypical hyperplasia. Conclu-sion Gastric hyperplastic polyp pits with type E and type F ,their results of pathology to display atypical hyperplasia.

Objective To observe the analgesic effect of progressive muscle relaxation training (PMR) and compound lidocaine cream in hemodialysis patients undergoing puncturing on arteriovenous fistula.Methods 96 hemodialysis patients undergoing puncturing on arteriovenous fistula were randomly divided into the progressive relaxation training group (group A),compound lidocaine cream group (group B),progressive relaxation training and compound lidocaine cream group (group C),and the blank control group (group D),with 24 patients in each group.Compound lidocaine cream were applied on the central puncture point 60 minutes before operation in group B and group C,and the patients of group A and group C were conducted with progressive relaxation training before puncture and sleep,while no intervention were assigned to group D.The pain of each patients were measured in 10 minutes with Visual Analog Scale(VAS).Results The pain scores were respectively (3.042 ± 0.999) in group A,(2.500 ±0.978)in group B,(1.333 ± 0.817) in group C and (5.042 ± 1.122) in group D.The AVOVA under the significant level of 0.05 for factorial date indicated that the main effect of factor A (progressive relaxation training) was statistically significant and the main effect of factor B (compound lidocaine cream) was also statistically significant.And that statistically significant interaction between the two factors was found,too.Conclusions Compound lidocaine cream with progressive relaxation training could alleviate the pain during puncturing arteriovenous fistula from hemodialysis significantly and was better than single use of each method.

Twelve methods of histochemical staining of mucins were compared,and improvemen ts in staining technic was made. Appropriate sodium meta-bisulfite was added to the ineffective Schiff's reagent to recover its staining property. The concentration of alcian blue was decreased from 1 % to 0.5%. Neutral red as the contrast stain was replaced by VG. The result of this study showed that this series of staining technics was simple to carry out, low cost, more specific, and suitable for any pathology department to use for detection of mucin in biopsy specimens of gastric mucosa.

One hundred and eleven cases of male reproductive diseases including epididymis,testi tis,cyst of epididymis,cryptochidism and varicocele were scanned with 10 MHz bifunctional soft probe.Utilization of high frequency probe may differentiate distinctively testitis from hemorrhage of testis and may easily find the locatipn and structrure of the eryptochidism.The combined use of 2 dimensional ul-trasonography and Doppler technique may be helpful fo detect the extent of varicocele and to evaluatethe postoperative efficacy.High resolution ultrasonography has been an important method for diagnos-ing the scrotal disease and the main indicator to decide whether the surgical or nonsurgical treatment should be undertaken.

This paper presents 231 cases of carcinoma in the region of the gastric cardia.In 96 of the cases-gastrectomy specimens were obtained,and for the remaining cases biopsy specimens were taken through gastroscopy.The study of cancerous cells in their developing stages in the tissues taken from the neighborhood of the tumours revealed that there were 4 types of histogenesis of gastric-cardia carcinoma:1.cancerous degeneration of cells at the glandular necks in the gastric foveolae;2.cancerous degeneration of a single cell or a few cells of the glandular epithelium;3.dysplasia of the epithelium with-malignant change;4.intestinal metaplasia of the epithelium with cancerous change.It is suggested that dysplasia of the epithelium and intestinal metaplasia of the epithelium accompanied by dysplasia are significant precancerous changes.

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Kidney Neoplasms ; blood ; pathology ; Male ; Neoplasm Metastasis ; Vascular Endothelial Growth Factor A ; blood ; physiology ; Wilms Tumor ; blood ; pathology

As a high sensitive biomarker of lung cancer ,IGFBP2 plays an important role in the occur-rence,development and treatment of lung cancer .Studies have showed that it promotes tumor progression by acti-vating the IGF1R and integrin mediated signal transduction pathway .Recent researches have demonstrated that it participates in targeted therapy of lung cancer ,which may become a new target for the treatment of lung cancer . This paper makes a review on the relationship between IGFBP 2 and lung cancer .

ObjectiveTo investigate p38 MAPK protein expression in human breast tumors and the regulation of preoperative chemotherapy. MethodsWe assessed p38 MAPK protein in 50 cases of breast cancer and adjacent normal tissue and that of breast benign tumors in 14 cases by Western blot. 15 cancer patients received preoperative CAF or TA protocol. Results The p38 protein level in tumors was significantly greater than that in the normal tissues, while there was not significant difference between benign tumors and corresponding normal tissue. p38 protein T/N ratio was higher in patients with lymph node metastasis than patients without( P

Objective:To figure out the characteristics of annual medical expenditure for rural residents and its distribution among different population.Methods:The individual annual medical service utilization information was summarized after dealing with the database of the 2014 new rural cooperative medical system by Excel.It prescribed the clustering of medical expenditure by Lorenz curve and Gini index.Descriptive statistics and x2 test were used to compare the characteristics and distribution of the annual medical expenditure among rural residents.The Chi-square test was conducted to compare the differences of the population distribution.Results:The costliest 5％ patients accounted for approximately 68％ of total health care expenditure.The annual total expenditure was 16628 yuan per capita among high-cost population,while low-cost population were 108 yuan.The Gini index of medical expenditure for rural residents was 0.81.Among female and population over 45 years old,the proportion of high-cost population was higher than 5％.People living near provincial road,in flatlands or areas where the ability of township hospital was limited were nore likely to expense higher medical costs,known as 6.40％,5.50％ and 5.80％ separately.Conclusion:Clustering of medical expenditure for rural residents was remarkable,while the expenditure among high-cost,medium-cost and low-cost population were quite different.The elderly,female and primary population living in areas where the ability of township hospitals were limited were more likely to generate high medical costs.