OBJECTIVE: To establish an HPLC method for the determination of chloramphenicol content in chloramphenicol vaginal soft capsule.METHODS: The sample was separated on Kromasil C18 column with 0.1% sodium heptanesulfonate solution-acetonitrile(77∶23) as mobile phase at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 272 nm;The sample size was 10 ?L and the column temperature was maintained at 30 ℃.RESULTS: The linear range of chloramphenicol was 0.079～0.113 mg?mL-1(r=0.999 6) and its average recovery rate was 99.38%(RSD=0.42%).CONCLUSION: The method was proved to be specific,sensitive,accurate in results and suitable for the determination of chloramphenicol content in chloramphenicol vaginal soft capsule.

This paper presents 231 cases of carcinoma in the region of the gastric cardia.In 96 of the cases-gastrectomy specimens were obtained,and for the remaining cases biopsy specimens were taken through gastroscopy.The study of cancerous cells in their developing stages in the tissues taken from the neighborhood of the tumours revealed that there were 4 types of histogenesis of gastric-cardia carcinoma:1.cancerous degeneration of cells at the glandular necks in the gastric foveolae;2.cancerous degeneration of a single cell or a few cells of the glandular epithelium;3.dysplasia of the epithelium with-malignant change;4.intestinal metaplasia of the epithelium with cancerous change.It is suggested that dysplasia of the epithelium and intestinal metaplasia of the epithelium accompanied by dysplasia are significant precancerous changes.

Twelve methods of histochemical staining of mucins were compared,and improvemen ts in staining technic was made. Appropriate sodium meta-bisulfite was added to the ineffective Schiff's reagent to recover its staining property. The concentration of alcian blue was decreased from 1 % to 0.5%. Neutral red as the contrast stain was replaced by VG. The result of this study showed that this series of staining technics was simple to carry out, low cost, more specific, and suitable for any pathology department to use for detection of mucin in biopsy specimens of gastric mucosa.

One hundred and eleven cases of male reproductive diseases including epididymis,testi tis,cyst of epididymis,cryptochidism and varicocele were scanned with 10 MHz bifunctional soft probe.Utilization of high frequency probe may differentiate distinctively testitis from hemorrhage of testis and may easily find the locatipn and structrure of the eryptochidism.The combined use of 2 dimensional ul-trasonography and Doppler technique may be helpful fo detect the extent of varicocele and to evaluatethe postoperative efficacy.High resolution ultrasonography has been an important method for diagnos-ing the scrotal disease and the main indicator to decide whether the surgical or nonsurgical treatment should be undertaken.

Objective To explore the clinical significance of gastric hyperplastic polyp pits under magnifying endoscopy. Methods Summary confirmed by magnifying endoscopy and pathological examination gastric hyperplastic po]yp's performance of magnifying endoscopy,Hp positive and the results of pathology during February 2004 to De-cember 2006. Results 55 patients found 116 gastric polyp,41.4% (48/116) with type A,26.7% (31/116) with type B,19. 8% (23/116) with type C,8. 6% (10/116) with type D,2. 6% (3/116) with type E,one case gastric pits blurred(type F) 0. 9% (1/116). 4 cases with type E and type F found moderate-severe atypical hyperplasia. Conclu-sion Gastric hyperplastic polyp pits with type E and type F ,their results of pathology to display atypical hyperplasia.

Objective To observe the analgesic effect of progressive muscle relaxation training (PMR) and compound lidocaine cream in hemodialysis patients undergoing puncturing on arteriovenous fistula.Methods 96 hemodialysis patients undergoing puncturing on arteriovenous fistula were randomly divided into the progressive relaxation training group (group A),compound lidocaine cream group (group B),progressive relaxation training and compound lidocaine cream group (group C),and the blank control group (group D),with 24 patients in each group.Compound lidocaine cream were applied on the central puncture point 60 minutes before operation in group B and group C,and the patients of group A and group C were conducted with progressive relaxation training before puncture and sleep,while no intervention were assigned to group D.The pain of each patients were measured in 10 minutes with Visual Analog Scale(VAS).Results The pain scores were respectively (3.042 ± 0.999) in group A,(2.500 ±0.978)in group B,(1.333 ± 0.817) in group C and (5.042 ± 1.122) in group D.The AVOVA under the significant level of 0.05 for factorial date indicated that the main effect of factor A (progressive relaxation training) was statistically significant and the main effect of factor B (compound lidocaine cream) was also statistically significant.And that statistically significant interaction between the two factors was found,too.Conclusions Compound lidocaine cream with progressive relaxation training could alleviate the pain during puncturing arteriovenous fistula from hemodialysis significantly and was better than single use of each method.

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Kidney Neoplasms ; blood ; pathology ; Male ; Neoplasm Metastasis ; Vascular Endothelial Growth Factor A ; blood ; physiology ; Wilms Tumor ; blood ; pathology

As a high sensitive biomarker of lung cancer ,IGFBP2 plays an important role in the occur-rence,development and treatment of lung cancer .Studies have showed that it promotes tumor progression by acti-vating the IGF1R and integrin mediated signal transduction pathway .Recent researches have demonstrated that it participates in targeted therapy of lung cancer ,which may become a new target for the treatment of lung cancer . This paper makes a review on the relationship between IGFBP 2 and lung cancer .

Objective To observe the effect of ultrafiltrated components of three kinds of serum containing phlegm-resolving prescriptions (Huanglian Wendan Decoction, Gualou Xiebai Banxia Decoction, and Erchen Decoction, respectively) on viability of human leukemic monocytic cell line THP-1 derived macrophages and macrophage differentiation to foam cells. Methods THP-1 cells were induced to differentiate into macrophages with phorbol-12-myristate-13-acetate ( PMA) . And then the achieved macrophages were used for the establishment of the atherosclerotic foam cell model after co-incubation with oxidized low density lipoprotein ( ox-LDL ) . Three kinds of serum containing phlegm-resolving prescriptions and blank serum were prepared and ultrafiltered by 10 KD ultrafiltrator, and then were co-cultured with the foam cell model, respectively. The viability of macrophages and the macrophage differentiation to foam cells were tested by methyl thiazolyl tetrazolium ( MTT) assay and oil red staining. Apoptotic rate of macrophages was determined by flow cytometry. Results After THP-1 cells were induced by PMA (100 nmol/L) for 20 hours and then were co-incubated with ox-LDL (50 μg/mL) for 24 hours, a typical atherosclerotic foam cell model was established successfully. The ultrafiltrated components of serum containing Huanglian Wendan Decoction had obvious effects on increasing the macrophage survival rate, decreasing macrophage apoptotic rate, and inhibiting macrophage differentiation to foam cells. The ultrafiltrated components of serum containing Gualou Xiebai Banxia Decoction or Erchen Decoction had an effect on enhancing the macrophage differentiation to foam cells but had no anti-apoptosis action. Conclusion Huanglian Wendan Decoction has anti-atherosclerosis action, which may be related to resisting macrophage apoptosis and decreasing macrophagic lipid-endocytosing function. Erchen Decoction and Gualou Xiebai Banxia Decoction are effective on increasing macrophagic lipid-endocytosing function.

Objective:To prepare a colloid gold kit for the qualitative detection of the dopes met-amphetamine and morphine by one-step chromatography immunoassay in human samples based on the principle of the highly specific immunochemical reactions between the antigens and their antibodies which were used for the analysis of specific compounds in human urine samples at ideal cut-off concentrations.Methods:The monoclonal antibodies(McAb) of either mouse anti-morphine or mouse met-amphetamine were prepared by hybridomas and the limiting dilution respectively. The titers of McAb were tested by ELISA.The kit was assembled and the specificity,sensitivity,and stability were investigated.In addition,these characters were compared with those of issued Pan Probe single test strip.Results:①The titer of the first McAb against morphine was 1∶3.2?103, as for another, 1∶1.6?103. ②The colloid gold kit was found with following cut-off for the dope concentrations,morphine at 300 ng/ml and met-amphetamine at 1 000 ng/ml. ③The colloid gold kit showed fine specificity without cross-reaction with routine medicines for cold treatment or with the solf-drinks of cokocola or with tobacco for the individuals who did not take the dopes the 7 days.④The colloid gold kit was even more sensitive than the issued strip,because urine sample of the junkies were still positive 4-5 days post-intake.Conclusion:The colloid gold kit for indentifying the drug-users is a rapid,specific, sensitive immunoassay suitable for the simultaneous, qualitative detection of drug abuse.