Objective To investigate the clinical significance of ECG QRS wave extension of the time limit for heart failure patients.Methods A retrospective analysis of heart failure patients electrocardiogram QRS complex duration and ultrasound heartbeat graph parameters were conducted,and according to the ECG QRS complex duration the patients were divided into QRS ≥ 120ms group and Q RS ＜ 120ms group,The cardiac functional grading,ultrasound heartbeat graph parameters,incidence rate of malignant arrhythmia,mortality within 1 year were compared between the two groups,in order to explore the possibility of QRS complex duration and the United States of America New York Heart Association(NYHA) classification of cardiac function,left ventricular end diastolic diameter(LVEDd),left ventricular ejection fraction(LVEF),left ventricular fraction shortening(LVFS),malignant arrhythmia incidence,mortality.Results No significant difference was found in the cardiac function between QRS ≥ 120ms group and QRS ＜120ms group(t =0.00,0.10,0.11,all P ＞ 0.05).LVEDd of ECG QRS complex duration ≥ 120ms group was significantly higher than that of ECG QRS complex duration ＜ 120ms group (t =3.31,P ＜ 0.05).In addition,LVFS value and LVEF value of ECG QRS complex duration≥ 120ms group were significantly lower than those of ECG QRS complex duration ＜ 120ms group (t =17.05,3.54,all P ＜0.05).However,LAD between the two groups had no significant difference(t =0.09,P ＞ 0.05).40 cases in ECG QRS complex duration ≥ 120ms group,8 cases had malignant arrhythmia in the hospital,6 cases of cardiac death,2 patients died of pulmonary infection.In ECG QRS complex duration ＜ 120ms group,4 cases had malignant arrhythmia during hospitalization,3 cases of cardiac death,1 cases died of septic shock.The incidence rate of malignant arrhythmia and mortality within 1 year of ECG QRS complex duration ≥120ms group were significantly higher than those of ECG QRS complex duration ＜ 120ms group (x2 =4.01,P ＜0.05).In the ECG QRS complex duration ≥ 120ms group,LVEDd of 6 cardiac death patients was higher than 70mm,LVEF was less than 30％.Conclusion Wave duration ≥ 120ms has nothing to do with the cardiac function grade,gender,age,heart failure,but relate with the incidence rate of malignant arrhythmia and mortality within 1 year,which can be used to evaluate disease severity and prognosis.

OBJECTIVE:To explore the problems and improvement measures in the process of operation and management of B2C pharmaceutical E-commerce enterprise,and to promote their development. METHODS:The performance evaluation index sys-tem was built preliminarily,including 5 first-level indexes and 28 second-level indexes. Questionnaires survey was conducted among 16 experts by Delphi method,and the weight of every index was calculated by experts according to importance,familiarity and reference. The company performance was analyzed and judged objectively by fuzzy comprehensive evaluation method. The re-sults of Y enterprise performance evaluation were empirically analyzed. RESULTS & CONCLUSIONS:The performance evaluation index system has been built,including 5 first-level indexes,24 second-level indexes. Average expert authority degree of two rounds survey is 0.76(>0.70),showing survey result is credible. The performance of the company in the finance and popularization have more influence to the performance of B2C pharmaceutical E-commerce enterprise. The performance of Y company is outstanding in profit growth,site stability and security,suppliers development and maintain and other aspects;however,it is not good enough of performance in employee satisfaction,customer professional services,logistics and distribution quality and other aspects. It is sug-gested to establish logistics by B2C pharmaceutical E-commerce enterprise themselves,carry out whole-course monitoring to im-prove the quality of logistics and distribution;increase product and pharmaceutical knowledge training input and introduce talent with the background of medical education so as to strengthen professional service;survey the satisfactory degree of staff to working environment,working group,working content and other aspects,and adjust enterprise system or communicate with staff in order to improve satisfactory degree.

There are significant correlations between hyperuricemia and risk factors for stroke, including hypertension, diabetes, hyperlipemia, and obesity, and it is also associated with ischemic stroke and its prognosis. This article reviews the correlations between hyperuricemia and risk factors for stroke and its influence on the prognosis of ischemic stroke.

Functional magnetic resonance imaging(fMRI) is a very powerful method to understand the mechanism of brain function with non-invasive localization, high spatial and temporal resolution. The essential fMRI technique is blood oxygen level dependent (BOLD-fMRI). Basic principles and methodological applications of BOLD-fMRI are reviewed in this paper.

Hyperlipidemia rats were fed by purified oil of Anguulla japonica (127. 7, 255. 4 and 510. 8 mg/kg) reduced significantly serum total cholesterol (TC), trigliceride (TG) and low density Hpoprotein(LDL) level. Effects of 127. 7 mg/kg were not significant. TC reduced rats of three doses were 13. 3%,37. 1% and 48. 7% respectively; TG reduced rats were 9. 5,12. 9 and 25. 2%; LDL reduced rats were 5. 1, 47. 4 and 62.2%. Also, doses of 255. 4 and 510. 8 mg/kg increased significantly high density lipoprotein(HDL) level. HDL increased rats of three doses were 8. 2,67. 3 and 61. 2%. Dose of 510. 8 mg/kg reduced significantly whole blood viscosity and plasma viscosity. Dose of 255. 4 mg/kg reduced significantly whole blood viscosity, but did not reduced plasma viscosity. A dose of 127. 7 mg/kg had no effects on both whole blood viscosity and plasma viscosity.

objective Microbial limit test(MLT)for Dahuang Zhechong pill was established and verified to supply scientific foundation for the establishment of MLT for antibacterial and anti-inflammatory Chinese patent medicine,and to supply principle guidance for the verification of the method issued in Chinese pharmacopoeia(2005 edition);meanwhile the antibiogram of Dahuang Zhechong pill was probed,which can provide evidence for its clinical administration.Methods With centrifugal sedimentation method and culture medium diluted method,the recovery test was done on each tested bacterial strain to validate the accuracy and feasibility of MLT.Results Dahuang Zhechong pill had a stronger inhibitory effect on staphylococcus aureus and bacillus subtilis,had a weak inhibitory effect on escherichia coli,and had no effect on candida albicans and aspergillus niger,indicating that the antimicrobial spectrum of Dahuang Zhechong pill is Gram-positive bacteria.Conclusion Centrifugal sedimentation method combined with culture medium diluted method can eliminate the anti-bacterial effect of Dahuang Zhechong pill during the experiment,thus the microorganisms in the polluted pill can be detected successfully.

Primovist is a newly developed hepatocytespecific agent that is transported into the hepatocyte through organic anion transporting polypeptides (OATPs) and excre ted into bile through canalicular multiorganic anion trans porters.Primovist uptake is usually reduced in HCC cells that present hypointensity on the hepatobiliary phase,there fore this agent may help differentiate HCC from other neoplasms and improve histological grading.This article will further explore the use of Primovist in liver cirrhosis and small hepatocellular carcinoma.

Wnt signaling pathway has a critical role in differentiation, proliferation, supersession and metabolism of osteocyte. Although the principal components are relatively simple, however, the numerous components consisted of Wnt signaling participate in the reaction, including several LRPs ( low density lipoprotein receptor-related protein,LRP) , 19 different Wnt proteins and 19 different FZDs( frizzled receptor, FZD) , and non-canonical Wnt signaling and multiple inhibitors of Wnt signaling.The Wnts linked to mem-brane coreceptor and activated downstream of canonical Wnt signaling or non-canonical Wnt signaling, In addition, different Wnts had different roles in differentiation, bone formation and bone absorption of osteocyte.This review aims to understand the relationship be-tween Wnt signaling and osteocyte metabolism and bone diseases, by analyzing a variety of factors and the process of Wnt protein and Wnt signaling pathway in activity of osteocyte.

AIM: To study the compatible stability of cefepime with 0.5% metronidazole injection.　METHODS: Dual wavelength spectrophotometry and K-ratio spectrophotometry were described for the determination of cefepime with metronidazole injection during 6 h. 　RESULTS: There was no evident variation in content during 6 h at room temperature. The average recovery rates of cefepime and metronidazole were (100.9±0.4)% and (99.8±0.6)% (n=5).　CONCLUSION: The compatiblity of cefepime with metronidazole injection is stable. The method of using dual wavelength spectrophotometry and K-ratio spectrophotometry to determine the compatible stability of cefepime with metronidazole is simple, accurate and usable.

Objective:To investigate the significance of T helper type 17 cells(Th17)/T regulatory cells (Treg) in the prognosis of chronic hepatitis B(CHB) by comparing the changes of Th17 cells ,Treg cells and their rate under different therapy in chronic hepatitis B patients.Methods: 40 patients infected with chronic hepatitis B(CHB) were divided into chronic hepatitis B without antiviral(CHBWA) group and chronic hepatitis B antiviral(CHBA) group depending on whether nucleoside antiviral was applied,with 20 cases each,10 cases in asymptomatic hepatitis b carriers(AsC) group.10 cases in health control (HC) group.Flow cytometer(FCM) was adopted to detect peripheral blood Th17 and Treg cell frequency in CHB patients at 0,1 month in CHBWA group ,at 0,1,3 month in CHBA group,AsC patients and HC cases ,then calculating Th17/Treg ratio and observing the dynamic changes at different disease states.Results: After the liver-protecting treatment in CHBWA patients,the Th17/Treg ratio at 1 month(0.39±0.11)decreased compared to 0 month(1.20±0.26)rapidly(P<0.01),and the ratio nearly reached the level of AsC and HC group (P>0.05).Ratio of Th17/Treg and ALT had good positive correlation (r=0.709,P=0).In CHBA patients group,after the liver-protecting and antiviral treatment,patients′ Th17/Treg ratio decreased rapidly in the first month(0.73±0.32) and third month(0.76±0.44),and there′s statistical difference compared with 0 month(1.18±0.27,P<0.01),but there was no significant change of Th17/Treg ratio after being treated for 3 months compared with 1 month,and the Th17/Treg ratios at 0,1,3 month were higher than that in AsC group and HC group(P<0.01).Ratio of Th17/Treg and ALT have good positive correlation (r=0.500,P=0.000),also the ratio of Th17/Treg and HBV DNA were positively correlated (r=0.345,P=0.007).Comparison of peripheral blood Th17,Treg cells frequency,Th17/Treg ratio between CHBWA and CHBA group at 0 month had no statistical significance(P>0.05),while the Th17/Treg ratio in CHBA group was higher than CHBWA group(t=4.471,P<0.01) after 1 month of treatment.Conclusion: In the CHB patients,antiviral treatment will influence the Th17,Tregs cells frequency,resulting the change of Th17/Treg ratio,but also the ratio may be associated with virus removal.