Aged ; Diastole ; Female ; Humans ; Tachycardia, Ectopic Atrial ; physiopathology ; therapy

Objective To analyze strains of Proteus mirabilis (P .mirabilis )and Proteus vulgaris (P .vulgaris ) isolated in a hospital,detect resistance to commonly used antimicrobial agents,and provide reference for rational ap-plication of antimicrobial agents in clinic.Methods 172 P .mirabilis isolates and 68 P .vulgaris isolates isolated between January 1 ,2011 and June 30,2013 were analyzed,antimicrobial resistance susceptibility testing were per-formed by disk diffusion method,data were analyzed with WHONET 5.4 software.Results P .mirabilis strains were mainly isolated from wound secretion(26.74%),sputum(22.68%)and urine(18.61 %),P .vulgaris were mainly from wound secretion(48.53%),urine(17.65%)and sputum(11 .77%).The resistance rates of P .mirabilis to ampicillin and trimethoprim/sulfamethoxazole were both>45.00%;the resistance rates of P .vulgaris to cefazo-lin and trimethoprim/sulfamethoxazole was 86.76% and 41 .18% respectively;the resistance rates of P .mirabilis and P .vulgaris to piperacillin/tazobactam,cefotaxime,ceftazidime,cefepime,carbapenems (ertapenem and mero-penem)and amikacin were all <20.00%.Conclusion The resistance rates of P .mirabilis and P .vulgaris to pip-eracillin/tazobactam,cefotaxime,ceftazidime,cefepime,ertapenem,meropenem and amikacin are all high,and can be used as the empirical medication for the treatment of related infection.

Animals ; Food Hypersensitivity ; genetics ; immunology ; metabolism ; Forkhead Transcription Factors ; genetics ; metabolism ; Glucocorticoid-Induced TNFR-Related Protein ; Mice ; Mice, Inbred BALB C ; RNA, Messenger ; genetics ; Receptors, Nerve Growth Factor ; genetics ; metabolism ; Receptors, Tumor Necrosis Factor ; genetics ; metabolism ; T-Lymphocytes, Regulatory ; metabolism

Objective To investigate the diagnostic value of combined detection of serum tumor markers,including CEA,CA125,neuron-specific enolase (NSE) and cytokeratin fragment antigen 21-1 (CYFRA21-1) for lung cancer patients.Methods The subjects involved 138 diagnosed lung cancer patients (82 males,56 females,average age 58.6 years,from October 2010 to March 2012),96 patients with benign lung diseases (56 males,40 females,average age 51.3 years) and 45 healthy adults (30 males,15females,average age 43.9 years).The pathological types of lung cancer consisted of 66 squamous cell carcinoma (SCC),52 adenocarcinoma and 20 small cell lung cancer (SCLC).The serum levels of CEA,CA125,NSE and CYFRA21-1 were measured with electrochemiluminescence immunoassay.The diagnostic efficacy for different pathological types was compared among each single tumor marker and combination of tumor markers.One-way analysis of variance q test were used for statistical analysis.Results The serum levels of CEA,CA125,NSE and CYFRA21-1 in patients with lung cancer were higher than those in patients with benign lung diseases and in healthy subjects (CEA:(19.99±30.99),(10.78±19.77),(3.25±3.42) μg/L;CA125:(79.70±95.98),(44.96±44.97),(20.66±7.13) μg/L; NSE:(35.23±40.22),(15.31±8.42),(13.30±5.65) μg/L; CYFRA21-1:(18.07±43.71),(8.30±8.83),(3.13±1.60) μg/L; F=4.481,5.436,4.776,6.002,all P＜0.05).The highest level of CEA,NSE or CYFRA21-1 were found in adenocarcinoma (F=4.932,P＜0.05),SCLC (F=5.119,P＜0.05) or SCC (F=5.378,P＜0.05),respectively.The highest sensitivity tumor markers for SCC,SCLC and adenocarcinoma were CYFRA21-1 (78.8％,52/66),NSE (75.0％,15/20) and CEA (57.7％,30/52),respectively.In combined detection,the highest sensitivity combinations for SCC,SCLC and adenocarcinoma were CEA+CYFRA21-1+NSE (89.4％,59/66),CEA+CYFRA21-1+NSE (80.0％,16/20) and CEA+CA125+NSE (78.8％,41/52),respectively.Conclusions Combined detection of serum tumor markers is more sensitive for the diagnosis of lung cancer.The expressions of the above four tumor markers is correlated with pathological types of lung cancer.

Objective To investigate the function of helper T lymphocyte cell(Th)1/Th2 cytokine in food allergy development.Methods A total 110 Balb/c mice were randomly divided into 2 groups:control group(40 mice)and food allergy group(70 mice).Food allergy animal models were established by ovalbumin,performed by skin prick test;in positive reaction mice,serum specific IgE,IL-4 and IFN-? were measured by enzyme linked immuosorbent assay(ELISA),and intestinal pathology were performed,and the mRNA expressions of IL-10,TGF-? in intestinal were measured by real-time PCR assay.Results In food allergy group,the mRNA expression of IL-10,TGF? in intestinal decreased(P

Objective To evaluate the impact factors on weight and proportion of prostate tissue resected by transurethral resection of prostate (TURP) in patients with benign prostatic hyperplasia (BPH). Methods The patients undergoing TURP from January 2007 to June 2009 and diagnosed as BPH according to postoperative pathological results were enrolled in this study. The prostate volume measured by transrectal ultrasound (TRUS), prostate specific antigen (PSA), maximum flow rate (MFR), residual urine volume and body mass index (BMI) were measured and calculated. The prostate tissue collected at resection was weighed, and the proportion of the prostate resected was the percentage of the pre-operative estimated weight. Results For the 458 patients with the average age of 69.5 years, average BMI was 24. 3 kg/m2 , PSA 6. 1 μg/ml, prostate volume 85.5 ml, MFR 8. 4ml/s, residual urine volume 31.8 ml, resected prostate weight 32.9 g, proportion of resection 37. 6%Prostate volume and BMI were positively related with resected prostate weight. BMI was positively related with proportion of prostate resection. There was no linear correlation between prostate volume and resected proportion. But there was significant difference in resected proportion between patients with prostate volume more than and less than 40 ml. Finasteride had no influence on the weight and resected proportion. Conclusions Prostate volume, PSA and BMI are correlated with weight and proportion of prostate tissue resected by TURP. Finasteride has no influence on the resected weight and proportion.

Objectives Selecting and constructing the biofilm -model of Pseudomonas aeruginosa in vitro.Observing the antibacterial activity of using Micafungin alone , or combined with Meropenem against Pseudomonas aeruginosa ( plankton-grown and biofilm-grown ) . Methods Ten clinical isolates of Pseudomonas aeruginosa were collected in July 2012, constructing the biofilm-model by microwell plate from Xiangya Hospital, Central South University.The ability of biofilm-formation of these strains was estimated by crystal violet colorimetric method, and optical microscope was used to observe the shape of the biofilm .MICs of Micafungin and Meropenem against plankton -grown and biofilm-grown Pseudomonas aeruginosa were tested by broth microdilution method, and the changes of MICs were compared.Using broth microdilution method, and connecting with the crystal violet colorimetric method , to observe the antibacterial effect of using Micafungin alone, or combined with antibiotics in the inhibition of the biofilm formation and destruction of mature biofilm of Pseudomonas aeruginosa.SPSS18.0 and t-test were used in comparing the differences between both treatment group and control group.P <0.05 showed the difference was statistically significant . Results Ten strains of Pseudomonas aeruginosa were successful in forming biofilms.Comparing with their planktonic counterparts, biofilms became more resistant to Meropenem , with the MIC raised 4-128 times. However, MIC of Micafungin could not be measured.Micafungin can inhibit the formation of biofilm in 9 experimental strains (PA1-PA9), where the minimum effective concentration of Micafungin were 156.25, 625, 10 000, 2 500, 1 250, 2 500, 1 250, 625 and 10 000 mg/L respectively.The absorbance values of the minimum effective concentration group and its positive growth control group were 0.342 ±0.020 vs 0.491 ±0.027, 0.512 ±0.018 vs 0.627 ±0.043, 0.862 ±0.021 vs 1.155 ±0.027, 0.731 ±0.028 vs 0.863 ± 0.017, 0.311 ±0.003 vs 0.447 ±0.021, 0.435 ±0.021 vs 0.597 ±0.011, 0.520 ±0.012 vs 0.605 ± 0.027, 0.611 ±0.059 vs 0.734 ±0.017, 0.223 ±0.011 vs 0.343 ±0.037 respectively, where the P values were 0.02, 0.03, 0.00, 0.01, 0.01, 0.00, 0.03, 0.01 and 0.03 respectively.The differences are statistically significant.Micafungin can damage the mature biofilm of 7 strains (PA1, PA2, PA4 -PA8), where the minimum effective concentration of Micafungin were 2 500, 2 500, 5 000, 2 500, 5 000, 2 500, 5 000 mg/L respectively.The absorbance values of the minimum effective concentration group and its positive growth control group were 1.459 ±0.014 vs 1.534 ±0.020, 1.279 ±0.020 vs 1.431 ±0.007, 1.365 ±0.024 vs 1.467 ±0.065, 1.322 ±0.028 vs 1.530 ±0.090, 0.920 ±0.004 vs 1.047 ±0.013, 1.860 ±0.005 vs 1.953 ±0.055, 1.407 ±0.005 vs 1.553 ±0.045 respectively, where the P values were 0.01, 0.01, 0.02, 0.01, 0.00, 0.03, 0.02.The difference is statistically significant.Micafungin combined with Meropenem applied in multiple drug resistant strains , which can inhibit the formation of biofilm better.Conclusions Micafungin can inhibit the formation Pseudomonas aeruginosa biofilm and damage the mature biofilms.Micafungin combined with Meropenem can act on multiple drug resistant strain , which may get a higher inhibition rate of the biofilm.

Catheter ablation of atrial fibrillation is an evolving field,especially for chronic atrial fibrillation.Several approaches have been performed in clinic practice,which include circumferential pulmonary vein ablation/isolation,left atrial linear ablation,CFAE ablation,pulmonary vein denervation or stepwise ablation.However,a standard approach has not been established.The total successful rates vary from 21% to 95%.The investigations in the mechanism of chronic atrial fibrillation and methods of three-dimensional mapping,and the usage of new source and technique of ablation will increase the successful rate,decrease the recurrence rate and procedure time.The long-term result of ablation of chronic atrial fibrillation and its impact on the structure and function of heart need further investigations.

Objective To investigate the usage of distortion product otoacoustic emissions(DPOAE) in normal infants hearing screening.Methods One hundred infants(200 ears) without clinical condition of illness were checked in GSI 70 DPOAE screening.The pure tones were 2,3,4 kHz,the OAE screener scored the test result as a pass,when the responses were above the PASS/REFER line and the signal-to-noise was at least 10 dB.Results Ninty-four infants(188 ears) passed hearing screening. Four infants(8 ears) with ceruminous impaction didn′t pass screening;after extracting impacted cerumen from the external auditory meatus,the 4 infants(8 ears) passed screening.Two infants(2 ears) with secretory otitis media didn′t pass screening,but after medicine treatment for 1 week,they passed screening too.Conclusion DPOAE has advantages in infants hearing screening.J Appl Clin Pediatr,2006,21(3):168-169

Abstract: Objective　To understand the density changes and seasonal fluctuation of larvae and adults of Aedes albopictus in Hainan Province from 2017 to 2021, and to provide a scientific basis for the prevention and control of dengue fever and other vector-borne diseases in Hainan Province. Methods　The Breteau index method was used to monitor the larvae of Aedes mosquitoes, and the breeding sites of all small indoor and outdoor water containers and their larvae was checked and recorded. The adult mosquitoes were monitored by double lamination and light trap. Results　A total of 1 962 adult female Aedes albopictus mosquitoes were captured in Hainan Province from 2017 to 2021, accounting for 2.17% of the total captured mosquitoes. There were significant differences in mosquito species composition in different habitats in different years (χ2=312.258, P<0.01). In 2017, the density of mosquitoes in livestock sheds was the highest (9.71 per lamp·night), but the composition ratio of Aedes albopictus was the lowest (1.29%). In 2020, the mosquito density in hospitals was the lowest (2.22 mosquitoes per lamp·night), and in 2019, the proportion of Aedes albopictus in residential areas and hospitals was the highest (3.27% on average).The mosquito density in different habitats was the lowest in 2020. The proportion of Aedes albopictus in residential areas and livestock sheds showed an increasing trend from 2017 to 2021, while the proportion of other habitats showed significant fluctuations. The proportion of Aedes albopictus in hospitals showed an obvious decreasing trend in 2020. From 2017 to 2021, the average mosquito density was 5.97, 4.26, 3.82, 3.39 and 3.68 mosquitoes per lamp·night, respectively, showing a decreasing trend year by year except 2021. Except for 2019, the lure index also showed a decreasing trend, with an average value of 1.5/(hour·net), showing a decreasing trend year by year. From 2017 to 2021, the annual average density of Aedes albopictus in Hainan Province was 0.09 mosquitoes per lamp·night, showing obvious seasonal fluctuation. The Breteau index showed a slow decline from 2017 to 2021, and its annual peak was between June and August, which was 1-2 months later than the peak of adult mosquito density. Conclusions　The population density of Aedes albopictus in Hainan shows obvious seasonal characteristics. Mosquito control activities should be carried out according to the seasonal characteristics of mosquitoes to reduce the risk of dengue fever and other infectious diseases.