Objective To screen the conservative,stable and specific DNA signature sequence in the plasmid of Yersinia pestis.Methods Specific validation trials and stability of the qualification test were carried out to 40 strains of Yersinia pestis,47 strains of non-Yersinia pestis of home and wild types of rodent in Yunnan,by using 32 DNA sequences derived from Yersinia pestis in the plasmid and conventional PCR technology,and Yersinia pestis vaccine strain EV76 as a positive control.Results Four pairs of relatively conservative,stable and specific DNA marker genes were screened:YPMT1.05c,YPMT1.03c,YPMT1.42 and YPMT1.04c.Conclusions The 4 pairs of Yersinia pestis DNA signature sequences can be used for rapid diagnosis of plague.

Aged ; Humans ; Male ; Palatal Neoplasms ; pathology ; Uvula ; pathology

Objective To determine the aberrant methylation status of RASSF1A,p16 and DAPK gene promoter region in induced sputum from lung cancer patients and the value of their combined detection in diagnosing lung cancers. Methods Methylation-specific PCR (MSP) was used to detect the promoter methylation status of RASSF1A,p16, and DAPK genes in induced sputum and pathological tissues from 82 patients with lung cancers and 25 patients with pulmonary benign lesion.We also analyzed the relation between methylation status and clinical pathological data.Results The positive rates of promoter methylation of RASSF1A,p16, and DAPK genes in pathological tissues from patients with lung cancers were 63.4%,59.8%, and 58.5%, respectively,and those in induced sputum were 54.9%,48.8%,and 51.2%, respectively. The promoter methylation of RASSF1A,p16, and DAPK genes were not detected in patients with pulmonary benign lesion.There was a significant difference between the lung cancer group and pulmonary benign lesion group (P＜0.05). The methylation rate of RASSF1A gene was significantly lower in the middle and high differentiation and non-metastastic lymph node of lung cancer tissues than that in the poor differentiation and the metastatic lymph node of lung cancer tissues(P＜0.05), and was not correlated with age, sex, smoking index, clinical stage, and pathological types.The methylation rate of p16, and DAPK genes was not significantly correlated with all the above mentioned factors (P＞0.05). The methylation rate of joint detecting RASSF1A, p16, and DAPK genes was 73.2%. Conclusion Joint detection for promoter hypermethylation of RASSF1A, p16, and DAPK genes in induced sputum may be used as a simple and effective index of the diagnosis and prognose of lung cancers, and can improve the positive rate.

Antibodies ; immunology ; Antibody Formation ; Complement C4b ; immunology ; pharmacology ; Diagnosis ; Graft Rejection ; diagnosis ; immunology ; Humans ; Kidney Transplantation ; immunology ; Peptide Fragments ; immunology ; pharmacology ; Transplantation, Homologous ; immunology

Objective To explore the constituent ratio and clinical characteristics of multifocal thyroid papillary carcinoma (MPTC).Methods The clinical data of 1616 cases of papillary thyroid carcinoma(PTC) were retrospectively analyzed from January 2002 to December 2011 of the First Affiliate Hospital of Dalian Medical University,which operated at the first time and confirmed by pathology.The change of constituent ratio of MPTC in PTC was analyzed and the differences of the clinical characteristics of the multifocal group and single focal group were analyzed.Results The constituent ratio of MPTC in PTC was increasing from 8.33％ (4/48) in 2002 to 30.38％ (96/316) in 2011 gradually.Compared to the single focal group,MPTC group had higher rate of neck lymph node metastasis(45.09％ vs 25.02％ ; P =0.000) and extrathyroidal invasion (20.95％ vs 9.04％ ;P =0.000).Compared with pure microcarcinoma,none pure microcarcinoma had higher rate of neck lymph node metastasis (P =0.000).More than two focuses has higher rate of neck lymph node metastasis than two focuses (P =0.000).The rate of recurrence with lymph node metastasis was higher than that without lymph node metastasis (24.05％ vs 8.98％ ;P =0.000) conformed by postoperative pathology.Conclusions The number and constituent ratio of MPTC in PTC is increasing gradually.MPTC group has high proportion of lymph node metastasis and extrathyroidal invasion than single focal group.None pure microcarcinoma has higher rate of neck lymph node metastasis than pure microcarcinoma;more than two focuses has higher rate of neck lymph node metastasis than two focuses.The recurrence rate is correlated with the rate of lymph node metastasis.

Objective To investigate the effects of high-affinity tyrosine kinase receptors TrkA, TrkC and the low-affinity neurotrophin receptor p75 (p75~ NTR) protein in laser-induced retinal injury. Methods The Wistar rats were anesthetized and exposed to frequency doubling Neodymium:Yttrium,aluminum garnet(ND:YAG,?=532nm) laser for 100 plus per eye, which were sacrificed on 12 hours,1,3,7,14 and 28 days after laser exposure and eyeball was taken out. We investigated retinal histology by hematoxylin and eosin staining, and the expression of TrkA, TrkC and p75~ NTR protein was studied by means of immunohistochemistry. Results Immunohistochemistry results showed a differential distribution for these three neurotrophin receptors in the laser injuried retina. TrkA was enhanced in nerve fiber layer (NFL), ganglion cell layer(GCL) and inner nuclear layer(INL) ,which is the most nuclei of the ganglion cells, proximal M?ller cell end feet and a little cells in the innermost part of the INL, its peak of up-regulation was after day 1-3, after day 28 there was sustained. Whereas TrkC and p75~ NTR expression was enhanced in the outer nuclear layer(ONL), which is distal M?ller cell processes, TrkC up-regulated after 12 hours, its peak was on day 1-3, after day 28 there was sustained, p75~ NTR up-regulated after 24 hours, its peak was on day 3, on day 14-28 there was sustained in the GCL, which is proximal M?ller cell processes. Conclusion The expression of TrkA, TrkC and p75~ NTR participated in the course of laser injuried retinal pathology.

Objective To investigate the effectiveness of panoramic mandibular index (PMI) in detecting the bone mineral density (BMD) of mandibular bone and whole-body bone in patients with chronic end-stage renal failure. Methods A total of thirty patients with peritoneal dialysis treatment were used as experimental group and 31 healthy adults were used as control group. The panoramic jaw tomography was taken for the measurement of superior PMI (sPMI) and inferior PMI (iPMI) in two groups. The dual-energy X-ray absorptiometry (DXA) was used to detect BMD of lumbar spine bone. Data were compared between two groups. The correlation of sPMI, iPMI and BMD of lumbar spine bone was analyzed. Results All indicators including sPMI(0.262 2 ± 0.026 7 vs 0.284 2 ± 0.025 4, t=3.301) , iPMI (0.314 1 ± 0.028 3 vs 0.334 1 ± 0.027 5, t=2.808) and BMD of lumbar spine bone (0.832 3 ± 0.101 0 vs 0.906 9 ± 0.113 6,t=2.709) were significantly lower in experimental group than those in control group (P＜0.01). There was a positive correlation between sPMI and iPMI with BMD of lumbar spine bone (r=0.439 and 0.389, P＜0.05). The BMD of lumbar spine bone was significantly lower in female patients than that of male patients in control group (0.849 7±0.114 7 vs 0.968 0±0.076 3,t=3.357). The BMD of lumbar spine bone was also significantly lower in female patients than that of male patients in experimental group (0.775 4±0.068 4 vs 0.882 1±0.099 9,t=3.365). There were no significant differences in values of sPMI and iPMI between male and female patients of two groups. Conclusion The BMD of mandibular bone is lower in patients with chronic end-stage renal failure than that of the normal people. PMI index is a simple and effective method to detect the BMD of mandibular bone, which can reflect the BMD of whole body bone in patients with chronic end-stage renal disease.

As a crucial part of the DNA damage repair process,the expression of excision repair cross-complementing group 1 (ERCC1 )is closely related to the genesis and development of gastric cancer.Studies find that ERCC1 gene polymorphism can alter the expression of the gene itself,which affects the sensitivity and efficacy of platinum-based chemotherapy.Therefore,the detection of ERCC1 polymorphism may guide the indi-vidualized chemotherapy of the patients with gastric cancer.

Objective To evaluate the diagnostic value of MSCT in small bowel obstruction and related complications caused by diverticulitis.Methods Clinical data and CT findings of 13 patients with diverticulitis and related complications were reviewed.The imaging findings of diverticulitis were calculated and compared with those in other 20 cases without intestinal obstruction.Results Among the 1 3 cases with diverticulitis,8 occurred in the j ej unum and other 5 in ileum.CT revealed the diverticulitis in all patients as a predominantly cylindrical expansion in communication with the adjacent small bowel.CT showed intraluminal intestinal contents and little air bubbles in 3 cases with simple diverticulitis,thickening of the appendix lumen in 2 cases with perforated diverticulitis, and annular high-density in lumen in 2 cases with diverticulitis and bezoar.CT also showed diverticulitis with volvulus in 6 cases as mesenteric swirling.The average thickness of diverticula were (3.06±0.31)mm,and the average volume of diverticula were (98.98±38.59)cm3 , exhibting statistically significant differences between diverticulitis and control groups (P value were 0.000 and 0.000).Conclusion MSCT is helpful for the diagnosis of small bowel obstruction and related complications caused by diverticulitis.

Ribosome inactivating protein(RIP) is a kind of toxin plant pr ot ein in which extensively lives in the body of higher plants and controls ribosom e's function. Beside it can control protein's combination,it has lots of biolog ical reactivity as resisting giving birth and tumor and controlling HIV. At fir st, RIP is isolated from seeds of bitter melon.The result of SDS-PAGE indicate s that there are lots of RIP in the abstraction liquid.Then we study the antivi ral action of RIP through the cell of CEF and SPF chicken embryos.The results s how that RIP can resist NDVF_48E_8,MDVCVI_988 and FPV-SD4 to so me extent.