Lentiviral vectors can transduce a wide range of different cell types.It can transduce not only dividing cells but also nondividing cells.It can be the immunotherapy tool for cancer therapy.Lentiviral yeators have several advantages,amongst which long-term expression,low toxicity,high capacity and low antivector immunity,they will play an important role and will attract more and more interest as an immunotherapeutic.Here,we discussed the clinical application and safety of the lentiviruses encoding tumor-associated antigen(TAA).

Objective:To study the influence of GPC3 gene on the proliferation,adhesion and invasion of hepatoma cell line SK-Hep-1.Methods:SK-Hep-1 cells were transfected with pEGFP-N2-GPC3 using Lipofectamine2000.RT-PCR was used to examine the GPC3mRNA expression in GPC3-SK-Hep-1 cells.MTT assay was used to examine the proliferation and calculate the adhesion rate of SK-Hep-1 cells.Transwell system was used to assess the migration and invasion of the cells.Results:SK-Hep-1 cells were successfully transfected with pEGFP-N2-GPC palsmid.GPC3 mRNA was detected in SK-Hep-1 cells.Transfection with CPC3 significantly suppressed the growth of SK-Hep-1 cells(P

Primovist is a newly developed hepatocytespecific agent that is transported into the hepatocyte through organic anion transporting polypeptides (OATPs) and excre ted into bile through canalicular multiorganic anion trans porters.Primovist uptake is usually reduced in HCC cells that present hypointensity on the hepatobiliary phase,there fore this agent may help differentiate HCC from other neoplasms and improve histological grading.This article will further explore the use of Primovist in liver cirrhosis and small hepatocellular carcinoma.

Objective To investigate the prevalence of hypertension and its related factors in Hulunbeier Mongolian region. Method A stratified multistage sampling was used to select residents aged 18 and over in a town of Hulunbeier region. 453 persons served as subjects for this questionnaire survey. Their blood pressure, height, weight, waist circumference were measured and body mass index (BMI) were calculated. Then an analysis of the prevalence of hypertension and its related risk factors were performed. Results The hypertension prevalence rate was 34.5%, the standardized rate was 45.4%, and the prevalence in men (48.9%) was significantly higher than that in women (25.4%) (P50 (OR=11.312, 95%CI 4.309~22.132), BMI (2.255, 1.180~4.311), gender (OR=2.788, 1.461~5.323) and drinking (2.306, 1.287~4.134) were significantly related to hypertension. Conclusion The standardized prevalence of hypertension in Hulunbeier Mongolian region is higher than the average prevalence in China, 2002. Old age, abdominal obesity, overweight or obesity, male and alcohol drinking were the main risk factors for hypertension in this region. The government and the health sector should strengthen the health education of healthy lifestyle and health promotion and the control.

Animals ; Antibodies, Anti-Idiotypic ; therapeutic use ; Disease Models, Animal ; Doxorubicin ; toxicity ; Immunotherapy ; Interleukin-18 ; immunology ; pharmacology ; Male ; Mice ; Nephrosis, Lipoid ; chemically induced ; pathology ; therapy

Objective:To investigate the expression of transcription factor BACH2 on PBMC in patients with coronary artery disease( CAD) and discuss its significance and role.Methods:The circulating levels of BACH2 and TLR4 in PBMC were evaluated by RT-PCR and Western blot separately.Serum levels of signature cytokines of T-helper cells,cytokines and ox-LDL were evaluated by ELISA.The data among groups were analyzed by one-way ANOVA.Correlation between variables were subjected to linear regression analysis.Results:The expression level of BACH2 down-regulated and TLR4 up-regulated significantly in PBMCs of ACS patients had significantly higher concentrations of serum ox-LDL,cytokines of TH1 cells,TH2 cells and TH17 cells and low level of cytokine of Treg cells.Negative correlation exists significantly between BACH2 and TLR4 in patients with CAD and between BACH2 and IFN-γ,IL-4 and IL-17,and between BACH2 and ox-LDL in serum.However, positive correlation exists significantly between BACH2 and TGF-β1.Conclusion:Increased serum concentrations of ox-LDL probably down-regulated the expression of BACH2 in ACS patients by activating TLR4 on PBMC, resulting in promoting production of pro-inflammatory cytokines and suppressing production of anti-inflammatory cytokine and then might cause ACS.

Calcium-Calmodulin-Dependent Protein Kinases ; antagonists & inhibitors ; Carcinoma ; pathology ; Cell Cycle ; drug effects ; Cell Line ; Cell Line, Tumor ; Enzyme Inhibitors ; pharmacology ; Female ; Flavonoids ; pharmacology ; Gene Expression Regulation ; drug effects ; Humans ; Ovarian Neoplasms ; pathology ; Proto-Oncogene Proteins c-fos ; genetics ; metabolism

The factors influencing cost accounting fall into such three categories as drivers and incentives,factors affecting authenticity and accuracy,and factors affecting application and selection.DRG payment,as it is getting more popular than before,is pushing forward cost accounting at an unprecedented pace.Thanks to the current payment system reform and the transition of public hospital reimbursement regime,such needs as exact payment,j ust compensation and cost/performance management of such hospitals are challenging the present cost accounting system,calling for the birth of a cost accounting system for these hospitals.To build such a system,micro-and macro-level goals need to be considered simultaneously,while needs of different levels should be designed,integrated and interacted with each other.Only in this way,goals can be reached for such a system.

Objective To analyze the growth and development condition of the children with cerebral palsy,and to investigate the influence of puberty on their adult height.Methods 56 children with cerebral palsy were selected as research subjects.34 healthy children were selected as control group.Their height and weight were measured,the development condition of their sexual character was checked,and then standard deviation of height and weight,height age and bone age were calculated,adult height and target height were predicted and analyzed.Results The standard deviation of height and weight in cerebral palsy group was (-1.29 ± 1.39) and (-0.77 ±1.20) respectively,which was lower than that of the normal control group[(0.40 ±0.95),(0.38 ± 1.01)] (t =-6.270,-4.6 7 6,all P ＜ 0.0 5).In preadolescent cerebral palsy group,the chronobiological age was bigger than their height age and bone age,the difference was significant (t =6.381,7.939,all P ＜ 0.05),but there was no significant difference between height age and bone age (P ＞ 0.05),there existed no significant difference between predicted adult height and target height(P ＞ 0.05).In adolescem cerebral palsy group,the chronobiological age and bone age were larger than their height age,which indicated significant difference (t =3.438,-3.759,all P ＜ 0.05),but there was no significant difference between the chronobiological age and bone age (P ＞ 0.05),the predicted adult height was lower than target height,the difference was significant (t =-5.204,P ＜ 0.05).Conclusion The growth and development of children with cerebral palsy would usually fall behind the normal children,but showed similarity in terms of starting age and process of puberty.After puberty,their bone age would increase dramatically,their predicted adult height would fall behind target height distinctly.

Pharmacological method state is a method to explain the principle of Chinese herb according to its external phenomenon such as shape, color, texture, features, and so on. The natural attribute of Chinese herb include shape, color, texture, smell, harvesting time, medicinal parts, chemical components, and so on. Though both of them have different key points, the natural attribute of Chinese herb can also be used to explain its medicinal mechanism. Therefore, the correlation research between pharmacological method state and the natural attribute of Chinese herb has some significance.
Biomedical Research ; Drugs, Chinese Herbal ; pharmacology