Objective To study the handicap of hearing and brain stem function in cerebral palsy children through determining their brainstem auditory evoked potential(BAEP).Methods BAEP were analyzed in 75 cases admissioned cerebral palsy children with MYTO type evoked potential equipment(made in Italy).Results The data showed that the abnormal BAEP rate was 72%(54/72),abnormity manifests as infaust differentiation of brainstem waves in diverse level,the latencies of wave Ⅰ,the interpeak latencies(IPLS) of wave Ⅰ～Ⅲ,Ⅲ～Ⅴ of BAEP in children with cerebral palsy were prolonged,the ratios of Ⅴ/Ⅰ wave amplitude was abnormal.Conclusion BAEP can be used to find out the changes of hearing handicap and brainstem function in children with cerebral palsy.

OBJECTIVE To study the bactericidal efficacy of glutaraldehyde,and its stability and change in pH value.METHODS The carrier quantity bactericidal test,field mimic bactericidal test,stability test,pH value detection,periodical biological surveillance and follow-up investigation of hospital infection were used.RESULTS After using of 20 000 mg/L of neutral glutaraldehyde on Candida albicans(ATCC 10231),Pseudomonas aeruginosa(ATCC27853),Escherichia coli(ATCC 25922) and other 3 clinical isolates separately for 3,5,and 10 min,the average killing rate was 97.90%,99.83% and 100.00%;on the field mimic bactericidal test with MRSA contaminated gastroscope,the average killing rate was 87.84%,99.50%,and 100.00%,respectively.The concentration of disinfectant after storaging 14d on 54℃ was decreased,only loss 1.46%,the concentration loss of disinfectant on using endoscope 5,7,and 14d after disinfection was 2.64%,0.69% and 0.94%,respectively.The value of pH was decreased from 7.22 to 6.00,6.50,and 6.20.The qualification rate of 260 various endoscopes after surveillance was 100.00%.Their was one of hospital infection happened among 3 200 patients after endoscopy which were followed up.CONCLUSIONS The results show that,only under the following circumstances,namely the concentration of glutaraldehyde being no less than 20 000mg/L and the action time being no less than 10 min,timely alteration of glutaraldehyde,and regular biological and concentration monitoring could the disinfection of endoscope be qualified so that hospital infection can be prevented,and medical dispute may be put to an end.

Objective To improve the hardware of a medical radioisotope dose calibration system base on LabVIEW platform.Methods Hiish performance voltage-frequency-converter VFC32 was applied to realize hardware circuit design of medical radioisotope dose calibrator,and the function of the circuit wag analyzed.The output voltage signal from the calibrator ionization chamber was processed by the designed circuit.The output frequency signal from the circuit was obtained by USB-6009 DAQ and was then processed by virtual instrument designed using LabVIEW 8.0.Results Experimental data graphs indicated that the improved system had a better linearity in the low and medium frequency band.Conclusion The redesigned radioisotope dose calibrator has advantages in improving measuring accuracy,measuring range,feeble-minded detection limit and so on.

This study examined the roles of HLA-G in the pathogenesis, development and immune tolerance of hemangioma. From 2000 to 2007, 52 paraffin-embedded specimens (26 from males and 26 from females) of skin capillary hemangioma and 7 samples of adjacent normal skin tissues were collected. Four fresh specimens of hemangioma were also harvested. All samples were HE-stained and proliferative cell nuclear antigen (PCNA) was immunohistochemically detected by using SP method. The samples were classified into proliferative group and degenerative group according to the Mulliken criteria and the expression pattern of PCNA. SP method and quantum dots double staining were applied to detect the expression of HLA-G and PCNA in hemangioma and normal tissue samples. The expression of HLA-G was detected by RT-PCR. The results showed that among the 52 samples of hemangioma, 29 were of proliferative type and 23 degenerative type, and of the four fresh samples of hemangioma, 2 were of proliferative type and 2 degenerative type. SP method results showed that HLA-G was expressed in both proliferative and degenerative hemangioma, but not in normal tissues. The quantum dots double staining exhibited that HLA-G expression was significantly higher in proliferative group than in degenerative (P<0.05) and normal groups (P<0.05), but there was no statistically significant difference between the latter two groups (P>0.05). RT-PCR revealed that HLA-G was transcribed in both the proliferative and degenerative hemangioma tissues, but not in normal tissues. We are led to conclude that the elevated expression of HLA-G in proliferative hemangioma cells may lead to immune tolerance, which allows cells to escape immune surveillance and proliferate. On the other hand, the lower expression of HLA-G in degenerative hemangioma may result in immune cells-induced degeneration of hemangioma.

Along with contemporary development of life support technology in perinatology and neo-natal intensive care,mortality of preterm infants has been reduced.However,the problems caused by the brain damage have been attracting more and more attention.The long-term outcome of prematures depends on their nervous system sequelae among the total complications.Preterm infants'brain damage mainly includes intravent-ricular hemorrhage(IVH)and periventricular leukomalacia(PVL).IVH and its complications are the main causes of early death,mental and physical developmental disorder of neonatals.Thus,the early diagnosis of brain dam-age,especially PVL,has become a serious problem that we are going to face.The scholars in or aboard hao payed close attention to these markers,such as MBP、S100B、ACT A、NF-L、NGB、MMP、IL-6、IL-10、IL-1 1 、NSE.The paper reviews these biological markers.

According to the recommendations of the International Medical lnformatics Association(IMIA)on education in biomedical and health informatics(first revision)published by IMIA in 2010 and combining the actuality of the basic clinical information skills and medical informatics education of Chinese medical students,the related contents of medical informatics have been integrated into the teaching course based on current medical information retrieval curriculum and medical informatics curriculum for non-medical informatics students of medical colleges and universities was constructed,so that they can meet the requirements of the basic information skills under information-based medical and healthy practice.

0.05],significantly higher than stage Ⅲ,Ⅳ[60%(6/10),50%(5/10);P

Adult ; Female ; Humans ; Logistic Models ; Male ; Mental Health ; statistics & numerical data ; Transients and Migrants ; psychology ; Young Adult

Objective To find a more suitable approach to pediatric anesthesia during laparoscopic operation to alleviate stress response.Methods Forty patients,1 to 3 years of age,ASA grade Ⅰ-Ⅱ,underwent general anesthesia(group GA)and combined general epidural anesthesia(group GEA)during their laparoscopic operation.Heart rate(HR),mean arterial pressure(MAP),ECG,SPO2,PETCO2,blood gas,plasma epinephrine,plasma norepinephrine,plasma IL-6 and IL-10 were monitered and recorded five minutes before anesthesia(T0),pre-pneumoperitoneum(T1),5 min after pneumoperitoneum(T2),30 min after pneumoperitoneum(T3),30 min after operation(T4)and 24 h after operation(T5).Results(1)HR,MAP of group GA were increased obviously at T2,T3,T4 compared to T0(P0.05).Significant difference was found at T1,T2,T3 and T4 between group GA and group GEA(t=4.749,10.349,13.632,10.051,P0.05).Plasma IL-10 at T1,T2,T3,T4 were higher than that at T0 in both groups(P