Objective To evaluate the clinical effect of treating chloasma with acupuncture and bloodletting therapy.Methods 176 cases with chloasma were recruited into two groups randomly. Acupuncture and bloodletting therapy was adopted in the treatment group (90 cases), and exclusive acupuncture was applied in the control group (86 cases). Results The effective rate was 96.7% in the treatment group and 51.2% in the control group. There was a significant statistical difference between two groups (P＜0.05). Conclusion The treatment of acupuncture and bloodletting therapy has a definite therapeutic effect on chloasma, being worthy of spread.

Objective To observe the clinical efficacy ofToutongning Formula in the treatment of migraine. Methods Totally 100 cases of migraine patients (wind disturbance, blood stasis syndrome) were randomly divided into two groups. Patients in the experimental group were treated withToutongning Formula orally, and patients in the control group were treated with flunarizine capsules 5 mg orally, for 30 d. The scores of number, degree, duration and accompanying symptoms of episodes before and after treatment were observed, the total scores were calculated, and adverse reactions were recorded, to evaluate the clinical efficacy and safety.Results Clinical total efficiency was 92.0% (46/50) in experimental group and 64.0% (32/50) in the control group; the incidence rate of adverse reactions was 20.0% (10/50) in experimental group and 80.0% (40/50) in the control group; there was statistical significant differences in efficacy and adverse reactions between the two groups (P<0.05). After the treatment, the differences in the scores of number, degree, duration and accompanying symptoms of episodes, and in the total scores between the two groups was statistically significant (P<0.05). There was statistical significance in the different value of above indexes before and after treatment between the two groups (P<0.05).ConclusionToutongning Formula can not only improve symptoms significantly in patients with migraine (wind disturbance, blood stasis syndrome), but also reduce adverse reactions, with more safety and efficacy than flunarizine capsules.

Aimed at the importance and current situation of hospital medical equipment management, the paper has analyzed the method of application and advantages of two dimension code technology and intelligent terminal in the management of medical equipment. And the two dimension code conversion software, which was developed by using C++ language compiler software and medical equipment management system, could achieve two dimension code information conversion and label production of medical equipment for instruction, maintenance, meterage and scraping and other information. And the intelligent terminal couldbe used to obtain information in real time, and it could combine with the existed one dimensional code to achieve informationization management for medical equipment, and then gradually replace one dimensional code. Through discussed the advantages of two dimension code and compared with the traditional model, the results indicated that this technology could provide a new direction in developmental trends and application prospect, and enhance the management level for dynamic information, and solve the problems of inconvenience in updating information.

Considering the fact that the critical medical education was relatively backward, and the basic medical education was out of touch with clinical practice, this study, combined with the complexity, uncertainty and comprehensiveness of critical care medicine , puts forward the concept of critical care medicine holistic medicine in clinical practice. We try a patient-oriented approach in clinical practice, focusing on the whole, focusing on the interaction relationship among patient and disease and its pathologi-cal and physiology processes, development, complication and prognosis of disease, instead of paying atten-tion only to disease or single organ itself. The purpose of patient-based learning is trying to train medical students and junior residents to form good clinical thinking ability and decision-making ability.

0.05),but correlated with age and invasion depth of tumor(P

Objective:To explore the clinical effect of intravenous immunoglobulin combined with conventional therapy in the treatment of severe pneumonia in infants and children.Methods:Selected the January 2015 to April 2016 in our hospital for treatment of 88 cases of children with severe pneumonia were retrospectively analyzed,which 47 cases by routine treatment plus intravenous immunoglobulin protein (treatment group),41 cases of children only take routine therapy (control group),with a course of 2 weeks.Results:The symptoms of fever,dyspnea,cough,wheezing,pulmonary rales were improved faster in the treatment group than the control group (P＜0.05);after 2 weeks of treatment,IgG levels were significantly higher in the treatment group than the control group(P＜0.05);after 2 weeks of treatment,the serum IL-6 and TNF-α levels were significantly lower in the treatment group than the control group(P＜ 0.05);after 1 week of treatment,57.45％ of patients was cured in the treatment groups while only 34.15％ patients in the control group (P＜0.05).Conclusion:Intravenous immunoglobulin combined with conventional therapy in the treatment of infants with severe pneumonia can significantly improve the theraputic effect and enhance blood immunoglobulin levels.

The identification of the specific cause in every patient has important clinical implications, because ischemic stroke is an etiologically heterogeneous disease. The Trial of Org 10172 in Acute Stroke Treatment (TOAST) classification can be used to define the etiology of stroke. However, TOAST classification can not be completed timely on admission of patients with acute stroke, which has impacted early guidance of clinical treatment. This article reviews the biological markers of early differential-diagnostic significance of the TOAST classification.

Objective:To construct NBS1 microRNA expressing eukaryotic recombinants,and identify biological activity of recombinants in Hela cell after transfection.Methods:According to sequence of NBS1mRNA,the NBS1 pre-microRNA was designed and synthesized,then cloned into the GFP reporter pcDNA6.2-GW/EmGFP-miR vector and transfected into Hela cell line.To detect integrity of inset fragment through colony PCR and sequencing analysis.The biological activity of recombinants through identify interference efficiency of NBS1 microRNA recombinants by way of Real-Time PCR and Western blot were determined.Results:Sequences of inset fragment in four microRNA expressing recombinants were correct.NBS1 mRNA and protein expression of four microRNA recombinants were decrease,which is the lowest in the NBS1mi-2 group.Conclusion:Four NBS1-targeting microRNA expressing recombinants all have biological activity in Hela cell line,and NBS1mi-2 recombinant has the most interference efficiency.The microRNA expressing plasmid which were successfully constructed and lay foundation for the studies on the tumor gene therapy of microrna targeting NBS1.

The carotene content in 42 kinds of Chinese herbs applied traditionally as the remedies for "Hwen-mong" were analyzed with the column chromatogra-ghic method. It was found that majority of the samples were not so rich in carotene as considered formerly, there were 13 kinds of herbs which contained no carotene at all (The contents of the 42 kinds tested varied from 0 to 12.83 mg. per 100 gm. of the dry weight). Our figures are far lower than those reported by Mar and Read in 1936. The significant discrepancy between these results is ascribed chiefly to the different methods employed for chemical analysis. Since the method used by Mar and Read did not completely exclude the interference of the other carotenoid pigments in the chloroform extracts.In view of the data obtained, we believe that the efficacy of these vegetable remedies for nightblindness due to a'vitaminosis A needs further investigation.