1.Identification of the Fungal Pathogen that Causes Strawberry Anthracnose in Bangladesh and Evaluation of In Vitro Fungicide Activity.
Md Shamim AKHTER ; Shahidul ALAM ; Md Shafiqul ISLAM ; Min Woong LEE
Mycobiology 2009;37(2):77-81
This study was conducted to identify the Colletotrichum species causing anthracnose disease of strawberry in Balgladesh and to evaluate in vitro activity of commercial fungicides it. Based on morphological and cultural characteristics, all 22 isolates were identified as Colletotrichum gloeosporioides. They developed white or glittery colonies with grey to dark grey reverse colony colors and they produced cylindrical conidia. The efficacy of five commercial fungicides, Bavistin DF, Dithane M-45, Sulcox 50 WP, Corzim 50 WP and Rovral 50 WP, were tested against the fungus. Bavistin inhibited radial growth completely and was followed in efficacy by Dithane M-45. In Bavistin DF treated media, the fungus did not produce conidia. The percent inhibition of radial growth of the fungus was increased with the increasing concentrations of fungicide.
Aminoimidazole Carboxamide
;
Bangladesh
;
Benzimidazoles
;
Carbamates
;
Colletotrichum
;
Cultural Characteristics
;
Fragaria
;
Fungi
;
Humans
;
Hydantoins
;
Maneb
;
Spores, Fungal
;
Zineb
2.In Vitro Effect of Fungicides, Plant Extracts and Smoke on Conidial Germination of Fusarium oxysporum Root Rot Pathogen of Piper betle.
Shahidul ALAM ; M Rafiqul ISLAM ; Montaz Ali SARKAR ; Arfatun Nahar CHOWDHURY ; M S ALAM ; Min Woong LEE
Mycobiology 2004;32(1):42-46
Five fungicides such as rovral, bavistin, cupravit, dithane M-45 and thiovit were tested against conidial germination of Fusarium oxysporum. Dithane M-45 was the most effective against the fungus. Rests of the fungicides were more or less effective in the inhibition of conidial germination after 5~30 minutes immersion in 500~2500 ppm concentration. Five plant extracts(leaf extracts of Ocimum sanctum, Lantana camera, Calotropis procera, Azadirachta indica and Vinca rosea) found to be more or less effective against the fungus. 'Dhup' somke have good inhibitory effect on conidial germination of F. oxysporum.
Azadirachta
;
Calotropis
;
Fungi
;
Fusarium*
;
Germination*
;
Immersion
;
Lantana
;
Ocimum
;
Piper betle*
;
Piper*
;
Plant Extracts*
;
Plants*
;
Smoke*
;
Vinca
3.Effect of Fungicides and Plant Extracts on the Conidial Germination of Colletotrichum gloeosporioides Causing Mango Anthracnose.
Ahmed IMTIAJ ; Syed Ajijur RAHMAN ; Shahidul ALAM ; Rehana PARVIN ; Khandaker Mursheda FARHANA ; Sang Beom KIM ; Tae Soo LEE
Mycobiology 2005;33(4):200-205
In Northern Bangladesh, generally mango trees are planted as agroforest that gives higher Net Present Value (NPV) than traditional agriculture. Mango anthracnose caused by Colletotrichum gloeosporioides Penz. is seen as a very destructive and widely distributed disease, which results in poor market value. Five fungicides such as Cupravit, Bavistin, Dithane M-45, Thiovit and Redomil were tested against conidial germination of C. gloeosporioides. Dithane M-45 and Redomil were the most effective when the conidia were immersed for 10~20 minutes at 500~1000 ppm concentrations. Antifungal activities of 13 plant extracts were tested against conidial germination of C. gloeosporioides. Conidial germination of C. gloeosporioides was completely inhibited in Curcuma longa (leaf and rhizome), Tagetes erecta (leaf) and Zingiber officinales (rhizome) after 15 minutes of incubation respectively.
Agriculture
;
Bangladesh
;
Colletotrichum*
;
Curcuma
;
Germination*
;
Ginger
;
Mangifera*
;
Plant Extracts*
;
Plants*
;
Spores, Fungal
;
Tagetes
;
Trees
4.Effects of Plant Extracts on Conidial Germination, Mycelial Growth and Sporulation of Fungi Isolated From Poultry Feed.
M Rafiqul ISLAM ; Shahidul ALAM ; M Ziaur RAHMAN ; S P CHOWDHURY ; M F BEGUM ; Nargis AKHTER ; M S ALAM ; Kee Don HAN ; Min Woong LEE
Mycobiology 2003;31(4):221-225
Effect of ethanolic extracts of Lawsonia inermis, Azadirachta indica, Vinca rosea, Tagetes patula, Ocimum sanctum, Colocasia antiquorum, Adhatoda vasica, Moringa oleifera, Datura metel and Curcuma longa leaf on conidial germination, mycelial growth and sporulation of Aspergillus flavus, A. niger and A. fumigatus were examined. The conidial germination of A. flavus and A. fumigatus were most inhibited by the extract of L. inermis, while that of A. niger was inhibited by A. indica. Other tested plant extracts have a good effect on conidial germination on the selected fungi. The highest mycelial growth of A. flavus (37 mm) was found in V. rosea, but in case of A. niger and A. fumigatus it (38 and 39 mm) was found in D. metel. The lowest (4, 9 and 6 mm) respectively mycelial growth of these fungi found in L. inermis. The highest sporulation (75 x 10(4)/ml) of A. flavus was counted in V. rosea, but in case of A. niger and A. fumigatus those (45 x 10(4) and 55 x 10(4)/ml) were in D. metel and the lowest (5 x 10(4), 12 x 10(4) and 9 x 10(4)/ml) respectively sporulation of these fungi counted in L. inermis plant extract medium.
Justicia
;
Aspergillus flavus
;
Azadirachta
;
Catharanthus
;
Colocasia
;
Curcuma
;
Datura metel
;
Ethanol
;
Fungi*
;
Germination*
;
Lawsonia Plant
;
Moringa oleifera
;
Niger
;
Ocimum
;
Plant Extracts*
;
Plants*
;
Poultry*
;
Tagetes
5.In vitro Inhibition Effect of Plant Extracts, Urine, Fertilizers and Fungicides on Stem Rot Pathogen of Sclerotium rolfsii.
Shahidul ALAM ; M Rafiqul ISLAM ; Montaz Ali SARKAR ; M S ALAM ; Kee Don HAN ; Jae Ouk SHIM ; Tae Soo LEE ; Min Woong LEE
Mycobiology 2004;32(3):128-133
Twenty plant extracts were tested against mycelial growth, sclerotium formatiom and dry weight of mycelium with sclerotia of Sclerotium rolfsii Sacc. The highest(90 mm) mycelial growth was measured in Adhatoda vasica, Tegetes erecta, Allium cepa, and Curcuma longa. The lowest(25 mm) was in Azadirachta indica. No mycelial growth was found in any concentration of cow, buffalo, and goat urine. The highest(90 mm) and the lowest(15 mm) mycelial growth were measured in Biomil and Urea, respectively. No mycelial growth was observed in Zinc. The highest(60 mm) and the lowest(2 mm) mycelial growth were recorded in Macuprex(Dodine; 65% WP) and Boron(100% Boric acid and 17% Boron) respectively. Mycelial growth was totally inhibited in Rovral(Iprodione; 50% WP).
Justicia
;
Azadirachta
;
Buffaloes
;
Curcuma
;
Fertilizers*
;
Goats
;
Mycelium
;
Onions
;
Plant Extracts*
;
Plants*
;
Urea
;
Zinc
6.In vitro Effects of Plant Extracts, and Phytohormones on Mycelial Growth of Anthracnose Fungi.
Shahidul ALAM ; Kee Don HAN ; Jae Min LEE ; Hyeon HUR ; Jae Ouk SHIM ; Kwang Choon CHANG ; Tae Soo LEE ; Min Woong LEE
Mycobiology 2004;32(3):134-138
Water extracts of six plants, such as Allium sativum, A. cepa, Zingiber officinale, Platycodon grandiflorum, Oenanthe javanica, and Capsella brusapastoris, were tested in vitro for inhibitory activity against mycelial growth of anthracnose fungi, Colletotrichum gloeosporioides, C. dematium, and C. coccodes. Among the plant extracts, an Allium sativum extract has good inhibitory effects in all the fungi. Four phytohormones namely, IAA (indole-3-acetic acid), NAA (a-Naphthyl acetic acid), 2,4-D (2,4-Dichloro phenoxy acetic acid) and BAP (Benzyl adenine purine) were used to find out the role over mycelial growth of these fungi. All the concentrations of BAP have good inhibitory effect against mycelial growth of these fungi than that of other tested plant hormones.
2,4-Dichlorophenoxyacetic Acid
;
Adenine
;
Capsella
;
Colletotrichum
;
Fungi*
;
Garlic
;
Ginger
;
Oenanthe
;
Plant Extracts*
;
Plant Growth Regulators*
;
Plants*
;
Platycodon
;
Water
7.Biological Screening of a Novel Nickel (II) Tyrosine Complex.
Md Rafiqul ISLAM ; S M Rafiqul ISLAM ; Abu Shadat Mohammod NOMAN ; Jahan Ara KHANAM ; Shaikh Mohammad Mohsin ALI ; Shahidul ALAM ; Min Woong LEE
Mycobiology 2007;35(1):25-29
A newly synthesized Nickel (II) tyrosine complex was screened as potential antimicrobial agent against a number of medically important bacteria (Bacillus subtilis, Streptococcus beta-haemolytica, Escherichia coli, Shigella dysenterae) and fungi (Aspergillus fumigatus, Candida albicans, Aspergillus niger, Aspergillus flavus, Penicillium sp.) strains. were used for antifungal activity. The antimicrobial activity was evaluated using the Agar Disc method. Moreover, the minimum inhibitory concentration of the complexes was determined against the same pathogenic bacteria and the values were found between 4~64 microg ml(-1). Brine shrimp bioassay was carried out for cytotoxicity measurements of the complexes. The LC50 values were calculated after probit transformation of the resulting mortality data and found to be 6 microg ml(-1).
Agar
;
Artemia
;
Aspergillus flavus
;
Aspergillus niger
;
Bacteria
;
Biological Assay
;
Candida albicans
;
Escherichia coli
;
Fungi
;
Mass Screening*
;
Microbial Sensitivity Tests
;
Mortality
;
Nickel*
;
Penicillium
;
Shigella
;
Streptococcus
;
Tyrosine*