2.Mechanism of action of neuregulin protecting the myocardium against daunorubicin-caused damage in rats.
Sha-yi JIANG ; Pei-ran MA ; Xiao-tian XIE
Chinese Journal of Pediatrics 2006;44(7):541-543
Animals
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Antibiotics, Antineoplastic
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administration & dosage
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toxicity
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Apoptosis
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drug effects
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Daunorubicin
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administration & dosage
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toxicity
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Disease Models, Animal
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Female
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In Situ Nick-End Labeling
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Male
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Myocardium
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cytology
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metabolism
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pathology
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ultrastructure
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Neuregulins
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metabolism
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pharmacology
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RNA, Messenger
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metabolism
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Rats
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Rats, Wistar
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Receptor, ErbB-2
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genetics
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metabolism
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Reverse Transcriptase Polymerase Chain Reaction
4.Effect of Combined Acupuncture-medication Anesthesia on Visceral Function in Laparoscopic Cholecystectomy
Li SHA ; Xiancheng KONG ; Xiaofeng XIE ; Jianping HUANG
Shanghai Journal of Acupuncture and Moxibustion 2015;(10):938-940
ObjectiveTo observe the effect of combined acupuncture-medication anesthesia on visceral function of patients undergone laparoscopic cholecystectomy, and to furtherexplore its clinical and theoretical significance.MethodSixty patients who were going to receive laparoscopic cholecystectomy were enrolled and randomized into an observation group (combined acupuncture-medication anesthesia) and a control group (conventional general anesthesia) by the random number table, 30 subjects in each group. The total bilirubin (TBIL), alanine aminotransferase (ALT), aspertate aminotransferase (AST), urea nitrogen, creatinine, and Mini-Mental State Examination (MMSE) were observed to evaluate the effect of combined acupuncture-medication anesthesia on visceral function of patients undergone laparoscopic cholecystectomy.ResultBefore operation, there were no significant differences in TBIL, ALT, urea nitrogen, creatinine, and MMSE between the observation group and the control group (P>0.05), while the AST level of the observation group was significantly higher than that of the control group before operation(P<0.05). On post-operation day 1, there were no significant differencesin comparing ALT and AST levels between the two groups (P>0.05); the TBIL level in the observation group was significantly lower than that in the control group (P<0.01); there were no significant differences in comparing the urea nitrogen and creatinine levels between the two groups (P>0.05). The MMSE score of the observation group was significantly higher than that of the control group 6 h after the operation (P<0.01). on post-operation day 1, there was no significant difference in comparing MMSE score between the two groups (P>0.05).ConclusionThe combined acupuncture-medication anesthesia and conventional general anesthesia play equal effects on liver and kidney functions in laparoscopic cholecystectomy, thus the two anesthesia methods are both safe andeffective. The combined acupuncture-medication anesthesia has certain protective effects on brain function in early stage, and possibly produces certain protective effectson liver function.
5.A pilot study on differentiation of human amnion mesenchymal stem cells into ocular surface epithelial cells by co-culture in vitro
Ye, WEN ; Xiangyin, SHA ; Li, SONG ; Zhiping, LIU ; Juan, PENG ; Lifei, XIE
Chinese Journal of Experimental Ophthalmology 2014;32(9):797-801
Background Recent studies indicated that human amnion mesenchymal stem cells (hAMSCs) can be induced to differentiate into multiple types of cells in vitro,but whether the hAMSCs can differentiate into ocular surface cells has not been reported yet.Objective This study was to investigate the feasibility of inducing differentiation of hAMSCs into ocular surface cells by co-culturing with human bulbar conjunctiva fibroblasts (hBCFs).Methods This study was approved by Ethic Committee of Affiliated Second Hospital of Guangzhou Medical University.HAMSCs were isolated from placenta under the informed consent of healthy delivery women.hAMSCs were cultured,passaged and identified by detecting the expressions of CD44,CD45,CD73,CD90 in the cells with flow cytometer,osteogenesis and adipogenic differentiation experiments.Human conjunctival tissue was obtained during the eye operation under the informed consent of patients and hBCFs were isolated and cultured with explant culture.The cells were divided into the hAMSCs culture group and the hAMSCs and hBCFs co-culture group and cultivated in Transwell chambers for 7 days.The expressions of cytokeratin 19 (CK19) and α-smooth muscle actin (α-SMA) in the cells were assayed by immnofluorescence technique.Results Cultured hAMSCs showed the slender shape and cell body enlarged with passage.CD44,CD73 and CD90 were expressed in the cells,and the expression of CD45 was absent.After 3-4 weeks of osteogenesis and adipogenic induce,the cells showed red staining for alizarin and oil red O.In the co-culture group of hAMSCs and hBCFs,hAMSCs presented the epithelioid cell-like in shape and showed the positive response for CK19 and weaker response for α-SMA.However,in the hAMSCs culture group,the cells showed the positive response for α-SMA and absent response for CK19.Conclusions The hAMSCs can differentiate into ocular surface cells after being induced by hBCFs.And the differentiation mechanism is possibly relevant to mesenchymal cells epithelium.
6.A Study of the inhibitory effect of a recombinant CEA vaccinia virus inocnlation on mouse CEA positive tumor
Qing ZHAO ; Sha TAO ; Jie YANG ; Hongbing YAO ; Jinwei XIE ; Chaoquan LUO
Journal of Cellular and Molecular Immunology 2001;17(4):324-327
Aim To explore antitumor mechanism of a recombinant vaccinia virus containing the human CEA-cDNA (rV-CEA). Methods C57/BL mice were immunized three times with rV-CEA. Six weeks later, the macrophages(MΦ s)and splenocytes from rV-CEA-immunized donors were transferred to CEA+ -HePa tnmor-bearing recipients,Meanwhile, the antitumor effects of these donor's MΦ s and splenocytes and that of the recipient's splenocytes were detected in vitro. Results The MΦ s and splenocytes from rV-CEA-immunized donors possessed strong antitumor activity in CEA-positive tumor-bearing recipients. The in vitro antitumor effect of splenocytes from mice inoculated with MΦ s from rV-CEA-immunized donors were markedly stronger than those from W-VV-immunized donors. However,the in vitro antitumor effect of the MΦ s from rV-CEA-immunized donors was the same as those from W-VV-immunized donors. Conclusion It is demonstrated that antitumor activity induced with rV-CEA may be mediated mainly by antigen present cells (the MΦ s), which activated tumor-specific T cells to kill tumor cells.
7.The treatment of post-operative complications after total arch reolacement for acute tvoe a aortic dissection
Ritai HUANG ; Song XUE ; Genxing XU ; Sha LIU ; Zhenlei HU ; Feng LIAN ; Bo XIE
Clinical Medicine of China 2011;27(12):1237-1239
Objective To describe the treatment experience of post-operative complications after total arch replacement for acute type A aortic dissection in 34 cases.Methods The subjects were 34 consecutive patients (Twenty-eight males and 6 females,age 34.0 -60.0 yrs) who received total arch replacement for acute Stanford type A aortic dissection from Jan.2005 to Oct.2010 in our hospital.The duration from the onset of the symptoms to the hospitalization ranged from 4 - 18 hrs.Pre-operative 2-D Echo revealed aortic valve regurgitation in 8 patients and mitral valve regurgitation in 1 patient.Results Three patients died after operation ( mortality 8.8% ).Severe complications included acute kidney injury in 13 cases,respiratory dysfunction in 12 cases,paraplegia in 1 case,mental disorder in 10 cases and excessive post-operative bleeding in 2 cases.Conclusion The incidence of the complications after total arch replacement is still high and severe.Intensive care should be stressed peri-operatively and early diagnosis and treatment for post-operative complications are important procedures.
8.Significance of the detection of serum levels of matrix metalloproteinases -1,-2,-3 and -9 in thoracic aortic diseases and acute myocardial ischemia
Feng LIAN ; Song XUE ; Ritai HUANG ; Sha LIU ; Zhenlei HU ; Bo XIE ; Zhenyang DAI
Clinical Medicine of China 2011;27(12):1248-1250
Objective To evaluate the clinical significance of the change of serum matrix metalloproteinases (MMP)-1,-2,-3 and -9 in acute and chronic aortic diseases and acute myocardial ischemia.Methods The blood serum levels of MMP-1,-2,-3 and -9 were detected in 30 patients with acute aortic dissection,19 patients with chronic aortic dissection,19 patients with aortic aneurysm and in 12 patients with acute myocardial ischemia,as well as in 16 healthy individuals who served as the control group.Serum MMP levels were measured by using an ELISA technique.Results There were significantly higher levels of MMP-3 in patients with acute myocardial ischemia as compared to acute aortic dissection ( [19.10 ± 3.11 ] μg/L vs [11.89 ± 1.31 ] μg/L,P =0.02).Significantly lower levels of MMP-1 were found in healthy controls compared to the groups of patients ( [1.30 ± 0.56 ] μg/L vs [2.99 ± 0.78 ] μg/L in acute aortic dissection,P =0.03,[3.12 ±0.78] μg/L in chronic dissection,P =0.02,[3.01 ± 1.01 ] μg/L in thoracic aortic aneurysm,P =0.03 and [5.01 ± 0.98 ] μg/L in acute myocardial ischemia,P =0.01 ).Higher levels of M MP-1 and MMP-3 were detected on males.There was a positive correlation between MMP-1 and increasing age ( r =0.38,P < 0.05 ).In patients operated for acute type A aortic dissection,the levels of MMP-1,MMP-3 and MMP-9 increased immediately after surgery,while the levels of MMP-2 decreased.Twenty-four hours after surgery levels of MMP-1,-2 and -9 were almost equal to the preoperative ones( P > 0.05 ).Conclusion Measurement of serum MMP levels in thoracic aortic disease and acute myocardial ischemia is a simple and relatively rapid laboratory test that could be used as a biochemical indicator of aortic disease or acute myocardial ischemia,when evaluated in combination with imaging techniques.
9.Treatment verification of helical tomotherapy intensity modulated radiation therapy
Shouping XU ; Xiaowu DENG ; Xiangkun DAI ; Lianyuan WANG ; Chuanbin XIE ; Ruigang GE ; Xiangyan SHA
Chinese Journal of Radiation Oncology 2008;17(5):395-397
Objective To evaluate the clinical feasibility of quality assurance of helical tomotherapy intensity modulated radiation therapy (IMRT) through a series of clinical case studies. Methods Tomotherapy planning system was used to provide optimized IMRT treatment plans. To verify the dose of IMRT plans,the cylindrical Virtual Water phantom,0.056cm3 AISL, ion chamber and EDR2 film were used for getting the absolute and relative doses from tomotherapy IMRT planning. The film and ion chamber were placed into the phantom. The doses of the interesting points and isodose distribution of the axial plane were measured,then the results were compared with those from the calculation in planning system for verification. Before the irradiation,kVCT images of the phantom were registered by tomotherapy MVCT images. So the setup of phantom was guided for verifying the position. Results The isedose distribution (on the axial plane) measured by the film was well consistent with that calculated by tomotherapy planning system. The error between the measured dose and predicted dose in the measured points was less than 3%. The setup error of the phantom was able to be kept within 1 mm. There was 2 mm offset along the vertical direction of couch from the virtual isocenter to beam isocenter,which should be considered during the phantom/patient setup. Conclusions The procedures for quality assurance of tomotherapy IMRT are feasible in our experience. And helical tomotherapy IMRT QA system has been constituted.
10.Dosimetric evaluation of carbon fiber tabletop on absorbed doses
Xiangyan SHA ; Yunlai WANG ; Xiongfei LIAO ; Chuanbin XIE ; Xiangkun DAI ; Lin MA
Chinese Journal of Radiation Oncology 2008;17(3):223-225
Objective To evaluate the dosimetric effect of carbon fiber tabletop on the patient doses in radiotherapy. Methods The transmission factors of couch and inserts were measured in air and solid water phantom using 0.6 cm3 ion chamber and PTW 2D ion chamber array for 6 MV ,10 MV and 18 MV X-ray,respectively. Absorbed doses at depth of maximum dose ,5 cm and 10 cm in solid water were measured with the 2D ion chamber array. Absorbed doses fluctuations with different gantry angles and air gaps between phantom and couch were also measured. Results The posterior field measurement showed that the reductions of absorbed doses at the depth maximum dose,5 cm and 10 cm were within 5%. The ratios of the absorbed doses with to without couch increased with the oblique incident angles and varied slightly with the air gap at depth of 5 cm. The transmission factors of inserts were less than those of couch owing to its thinner thickness. Conclusion The carbon-fiber tabletop affects the absorbed doses and dose distributions of the target, and this effect changes with the gantry angle and air gap. Special considerations should be taken during treatment planning.