1.Left-Sided Poland’s Syndrome without Limb Anomalies
Yeong-Il NA ; Seungbok LEE ; Seung-Min BAEK ; Jong Hoo LEE
Clinical Pain 2022;21(2):129-132
“Poland’s syndrome” is a rare congenital disease whereby defects can accompany the chest, nipple, chest wall, and extremities on one side of the body. We diagnosed a 19-year-old male patient who presented to another hospital for a routine physical exam before enlisting in the military and was suspected of having a left brachial plexus injury. His chief complaint was the flatness of the left anterior chest wall without any significant functional inconvenience. Aplasia of the pectoralis minor and costosternal portion of the pectoralis major was observed through physical examination and computed tomography (CT). The patient was diagnosed with left-sided Poland’s syndrome without any limb abnormalities. Poland Syndrome should be highly considered in patients presenting with bilateral chest wall imbalance in the absence of with a recent traumatic history.
2.Characterization of the Rho GTPase-Activating Protein RhoGAP68F.
Experimental Neurobiology 2011;20(1):29-34
Rho small GTPases control multiple aspects of neuronal morphogenesis by regulating the assembly and organization of the actin cytoskeleton. Although they are negatively regulated by GTPase activating proteins (GAPs), the roles of RhoGAPs in the nervous system have not been fully investigated. Here we describe a characterization of Drosophila RhoGAP68F that is mainly expressed in the embryonic central nervous system. RNA in situ hybridization analysis showed that expression of RhoGAP68F is highly restricted to the embryonic brain and ventral nerve cord. Database search revealed that RhoGAP68F contains an N-terminal Sec14 domain and a C-terminal RhoGAP domain. Rho-GTP pull-down assay demonstrated that the RhoGAP domain of RhoGAP68F inactivates RhoA but not Rac1 or Cdc42 in HEK293 cells. In addition, expression of RhoGAP68F in NIH3T3 cells suppressed LPA-induced stress fiber formation, which is mediated by RhoA. Finally, neuronal overexpression of RhoGAP68F causes synaptic overgrowth at the larval neuromuscular junction (NMJ). Taken together, these results suggest that RhoGAP68F may play a role in synaptic growth regulation by inactivating RhoA.
Actin Cytoskeleton
;
Actins
;
Brain
;
Central Nervous System
;
Drosophila
;
GTPase-Activating Proteins
;
HEK293 Cells
;
In Situ Hybridization
;
Monomeric GTP-Binding Proteins
;
Morphogenesis
;
Nervous System
;
Neuromuscular Junction
;
Neurons
;
RNA
;
Stress Fibers
3.Brain Activation Related to Perspective-Taking in Adolescents: Differences from Adults
Seong Kyoung PARK ; Jung Woo SON ; Seungbok LEE ; Hei Rhee GHIM ; Sang Ick LEE ; Chul Jin SHIN ; Siekyeong KIM ; Gawon JU
Journal of the Korean Society of Biological Psychiatry 2019;26(2):47-58
OBJECTIVES: The purpose of this study was to investigate the differences between adolescents and adults, in the perspective-taking ability, as well as the brain activation patterns during the perspective-taking situation.METHODS: We recruited healthy adolescents aged 13 years to 15 years (n = 20) and adults aged 19 years to 29 years (n = 20). All the subjects were scanned while performing the perspective-taking task, in which an emotional situation was presented in the form of statements comprising first person, as well as third person perspectives. Differences in brain activation between groups were assessed by contrasting neural activity during the tasks.RESULTS: In the between-group analysis, while performing the third-person perspective-taking task, the adolescent group showed greater neural activities in the middle frontal gyrus and precentral gyrus as compared to the adult group. Positive correlation was observed between the activity in the frontal areas (Brodmann area 6/9) and the score of scales related to perspective-taking and social cognition in the adolescent group.CONCLUSIONS: This study suggests that several frontal brain areas of adolescents needs to be overactivated in order to compensate for low perspective-taking ability when they ought to take another person's point of view.
Adolescent
;
Adult
;
Brain
;
Cognition
;
Frontal Lobe
;
Humans
;
Magnetic Resonance Imaging
;
Weights and Measures
4.Persistent Symptoms After Acute COVID-19 Infection in Omicron Era
Young Hee JUNG ; Eun-Hye HA ; Kang Won CHOE ; Seungbok LEE ; Dong Ho JO ; Wang Jun LEE
Journal of Korean Medical Science 2022;37(27):e213-
Background:
We aim to compare the clinical characteristics and subjectively reported symptoms of the acute coronavirus disease (COVID) phase and those of the post-acute COVID phase to examine varying factors that affect the number of persistent symptoms and their categories.
Methods:
We categorized 1,122 patients who visited the post coronavirus disease 2019 (COVID-19) clinic into two groups: “acute group” (< 4 weeks following diagnosis of COVID-19) and “post-acute group” (> 4 weeks following diagnosis of COVID-19). We statistically compared clinical characteristics between the two groups and determined which factors are associated with the number of persistent symptoms and their categories.
Results:
The persistent symptoms of post COVID-19 conditions were classified into three categories as follows: Category A (the prevalence of symptoms is higher in the acute-visit group than in the post-acute-visit group), Category B (the prevalence of symptoms is not different between the two groups) and Category C (the prevalence of symptoms is higher in the post-acute-visit group than in the acute-visit group). Category A mainly included respiratory symptoms. Category B had generalized weakness, weight loss, cardiologic symptoms, hypogeusia, hyposmia, anxiety, and various gastrointestinal symptoms. Category C included fatigue, decreased attention, depression, blurred vision, hair loss, and sexual dysfunction.Anxiety, depression, fatigue and age were also associated with the number of symptoms and their categories, and anxiety is the most correlated factor (P < 0.001) among them.
Conclusion
The persistent symptoms of post COVID-19 condition involve multi-organ and continue for four weeks or greater. Therefore, long-term observation and multidisciplinary interventions are essential for patients with post COVID-19 conditions.
5.Targeted resequencing of candidate genes reveals novel variants associated with severe Behcet's uveitis.
Sang Jin KIM ; Seungbok LEE ; Changho PARK ; Jeong Sun SEO ; Jong Il KIM ; Hyeong Gon YU
Experimental & Molecular Medicine 2013;45(10):e49-
Behcet's disease (BD) is a chronic systemic inflammatory disorder characterized by four major manifestations: recurrent uveitis, oral and genital ulcers and skin lesions. To identify some pathogenic variants associated with severe Behcet's uveitis, we used targeted and massively parallel sequencing methods to explore the genetic diversity of target regions. A solution-based target enrichment kit was designed to capture whole-exonic regions of 132 candidate genes. Using a multiplexing strategy, 32 samples from patients with a severe type of Behcet's uveitis were sequenced with a Genome Analyzer IIx. We compared the frequency of each variant with that of 59 normal Korean controls, and selected five rare and eight common single-nucleotide variants as the candidates for a replication study. The selected variants were genotyped in 61 cases and 320 controls and, as a result, two rare and seven common variants showed significant associations with severe Behcet's uveitis (P<0.05). Some of these, including rs199955684 in KIR3DL3, rs1801133 in MTHFR, rs1051790 in MICA and rs1051456 in KIR2DL4, were predicted to be damaging by either the PolyPhen-2 or SIFT prediction program. Variants on FCGR3A (rs396991) and ICAM1 (rs5498) have been previously reported as susceptibility loci of this disease, and those on IFNAR1, MTFHR and MICA also replicated the previous reports at the gene level. The KIR3DL3 and KIR2DL4 genes are novel susceptibility genes that have not been reported in association with BD. In conclusion, this study showed that target enrichment and next-generation sequencing technologies can provide valuable information on the genetic predisposition for Behcet's uveitis.
Adult
;
Aged
;
Behcet Syndrome/*genetics
;
Case-Control Studies
;
Female
;
Histocompatibility Antigens Class I/genetics
;
Humans
;
Intercellular Adhesion Molecule-1/genetics
;
Interferon-alpha/genetics
;
Male
;
Middle Aged
;
*Polymorphism, Single Nucleotide
;
Receptors, IgG/genetics
;
Receptors, KIR/genetics
;
Receptors, KIR2DL4/genetics
6.What is Neuroaesthetics? : A New Paradigm in Psychiatry.
Jung Woo SON ; Seungbok LEE ; Woo Hyun JUNG ; Sang Hyun JEE ; Seong Hoon JUNG
Journal of Korean Neuropsychiatric Association 2013;52(1):3-16
Neuroaesthetics is an area of research on the essence of aesthetic experience according to the neurobiological perspective. The purpose of this article is to introduce both the important results from some studies based on neuroaesthetics and some neuroaesthetic reports in specific psychiatric disease. Aesthetic experience is thought to be a series of cognitive and affective processes for pursuing aesthetic judgment and aesthetic emotion. According to the present neuroaesthetic studies, the important domains are reward system, embodiment, aesthetic perspective in contrast with practical perspective, sublime, and so on. The neuroaesthetic approach to specific psychiatric disease is just beginning; however, case studies on the sudden appearance of artistic talents in neuropsychiatric patients have been frequently reported. We expect that neuroaesthetics is likely to continue to contribute new knowledge regarding the ultimate causes of various psychiatric diseases and the creativity of many psychiatric patients.
Aptitude
;
Beauty
;
Brain
;
Creativity
;
Humans
;
Judgment
;
Reward
7.Development of ELISA-kit of Quantitative Analysis for Adiponectin and Their Correlation with Cardiovascular Risk Factors.
Sun Ha JEE ; Seungbok LEE ; Sungsik MIN ; Jungyong PARK ; Hyon Suk KIM ; Sang Yeun KIM ; Ji Eun YUN ; Sun Ju LEE ; Eun Jung JEE ; Hee Yeon LEE ; Hye Yun SONG
Korean Journal of Epidemiology 2007;29(2):165-175
PURPOSES: To develop a rapid, sensitive, qualitative ELISA-kit for serum adiponectin and examine correlation with adiponectin and cardiovascular risk factors. METHODS: On the base of monoclonal antibodies against adiponectin, apply indirect ELISA to study the performance parameter of the kit. The correlation was examined between adiponectin and cardiovascular risk factors including waist circumference, body mass index, triglyceride, and HDL cholesterol. RESULTS: The limited concentration of detection of the ELISA-kit was 1ug/ml. Linearity with R&D system and AdipoGen with this ELISA-kit was acceptable: the linear equation with R&D system was y=1.0116x + 0.4629 (R2=0.97) and linear equation with AdipoGen was y=0.9562x + 1.1961 (R2=0.93), respectively. The average recovery rate of the ELISA-kit ranged 92 to 104%. The correlation coefficient of waist circumference with adiponectin was -0.2276 (p<0.0001) among men and -0.2328 (p<0.0001) among women. CONCLUSION: This ELISA-kit was quick, sensitive, and stable and can be used to determine adiponectin in serum.
Adiponectin*
;
Antibodies, Monoclonal
;
Body Mass Index
;
Cholesterol, HDL
;
Enzyme-Linked Immunosorbent Assay
;
Female
;
Humans
;
Male
;
Risk Factors*
;
Triglycerides
;
Waist Circumference
8.Cognitive and Emotional Empathy in Young Adolescents: an fMRI Study
Eun Jin KIM ; Jung-Woo SON ; Seong Kyoung PARK ; Seungwon CHUNG ; Hei-Rhee GHIM ; Seungbok LEE ; Sang-Ick LEE ; Chul-Jin SHIN ; Siekyeong KIM ; Gawon JU ; Hyemi PARK ; Jeonghwan LEE
Journal of the Korean Academy of Child and Adolescent Psychiatry 2020;31(3):121-130
Objectives:
We investigated the differences in cognitive and emotional empathic ability between adolescents and adults, and the differences of the brain activation during cognitive and emotional empathy tasks.
Methods:
Adolescents (aged 13–15 years, n=14) and adults (aged 19–29 years, n=17) completed a range of empathic ability questionnaires and were scanned functional magnetic resonance imaging (fMRI) during both cognitive and emotional empathy task. Differences in empathic ability and brain activation between the groups were analyzed.
Results:
Both cognitive and emotional empathic ability were significantly lower in the adolescent compared to the adult group. Comparing the adolescent to the adult group showed that brain activation was significantly greater in the right transverse temporal gyrus (BA 41), right insula (BA 13), right superior parietal lobule (BA 7), right precentral gyrus (BA 4), and right thalamus whilst performing emotional empathy tasks. No brain regions showed significantly greater activation in the adolescent compared to the adult group while performing cognitive empathy task. In the adolescent group, scores of the Fantasy Subscale in the Interpersonal Reactivity Index, which reflects cognitive empathic ability, negatively correlated with activity of right superior parietal lobule during emotional empathic situations (r=-0.739, p=0.006).
Conclusion
These results strongly suggest that adolescents possess lower cognitive and emotional empathic abilities than adults do and require compensatory hyperactivation of the brain regions associated with emotional empathy or embodiment in emotional empathic situation. Compensatory hyperactivation in the emotional empathy-related brain areas among adolescents are likely associated with their lower cognitive empathic ability.
9.Neural Correlates of Cognitive and Emotional Empathy in Patients with Autism Spectrum Disorder.
Seungwon CHUNG ; Jung Woo SON ; Seungbok LEE ; Hei Rhee GHIM ; Sang Ick LEE ; Chul Jin SHIN ; Siekyeong KIM ; Gawon JU ; Sang Cheol CHOI ; Yang Yeol KIM ; Young Jin KOO
Journal of the Korean Academy of Child and Adolescent Psychiatry 2016;27(3):196-206
OBJECTIVES: Individuals with autism spectrum disorder (ASD) are considered to have problems with empathy. It has recently been suggested that there are two systems for empathy; cognitive and emotional. We aimed to investigate the neural response to cognitive and emotional empathy and elucidate the neurobiological aspects of empathy in patients with ASD. METHODS: We recruited patients with ASD (N=17, ASD group) and healthy controls (HC) (N=22, HC group) for an functional magnetic resonance imaging study. All of the subjects were scanned while performing cognitive and emotional empathy tasks. The differences in brain activation between the groups were assessed by contrasting their neural activity during the tasks. RESULTS: During both tasks, the ASD group showed greater neural activities in the bilateral occipital area compared to the HC group. The ASD group showed more activation in the bilateral precunei only during the emotional empathy task. No brain regions were more activated in the HC group than in the ASD group during the cognitive empathy task. While performing the emotional empathy task, the HC group exhibited greater neural activities in the left middle frontal gyrus and right anterior cingulate gyrus than the ASD group. CONCLUSION: This study showed that the brain regions associated with cognitive and emotional empathy in ASD patients differed from those in healthy individuals. The results of this study suggest that individuals with ASD might have defects both in cognitive empathy and in emotional empathy.
Autism Spectrum Disorder*
;
Autistic Disorder*
;
Brain
;
Empathy*
;
Gyrus Cinguli
;
Humans
;
Magnetic Resonance Imaging
10.A De Novo RAPGEF2 Variant Identified in a Sporadic Amyotrophic Lateral Sclerosis Patient Impairs Microtubule Stability and Axonal Mitochondria Distribution.
Keunjung HEO ; Su Min LIM ; Minyeop NAHM ; Young Eun KIM ; Ki Wook OH ; Hwan Tae PARK ; Chang Seok KI ; Seung Hyun KIM ; Seungbok LEE
Experimental Neurobiology 2018;27(6):550-563
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that is frequently linked to microtubule abnormalities and mitochondrial trafficking defects. Whole exome sequencing (WES) of patient-parent trios has proven to be an efficient strategy for identifying rare de novo genetic variants responsible for sporadic ALS (sALS). Using a trio-WES approach, we identified a de novo RAPGEF2 variant (c.4069G>A, p.E1357K) in a patient with early-onset sALS. To assess the pathogenic effects of this variant, we have used patient-derived skin fibroblasts and motor neuron-specific overexpression of the RAPGEF2-E1357K mutant protein in Drosophila. Patient fibroblasts display reduced microtubule stability and defective microtubule network morphology. The intracellular distribution, ultrastructure, and function of mitochondria are also impaired in patient cells. Overexpression of the RAPGEF2 mutant in Drosophila motor neurons reduces the stability of axonal microtubules and disrupts the distribution of mitochondria to distal axons and neuromuscular junction (NMJ) synapses. We also show that the recruitment of the pro-apoptotic protein BCL2-associated X (BAX) to mitochondria is significantly increased in patient fibroblasts compared with control cells. Finally, increasing microtubule stability through pharmacological inhibition of histone deacetylase 6 (HDAC6) rescues defects in the intracellular distribution of mitochondria and BAX. Overall, our data suggest that the RAPGEF2 variant identified in this study can drive ALS-related pathogenic effects through microtubule dysregulation.
Amyotrophic Lateral Sclerosis*
;
Axons*
;
Drosophila
;
Exome
;
Fibroblasts
;
Histone Deacetylases
;
Humans
;
Microtubules*
;
Mitochondria*
;
Motor Neurons
;
Mutant Proteins
;
Mutation, Missense
;
Neurodegenerative Diseases
;
Neuromuscular Junction
;
Skin
;
Synapses