The nucleolus of human cell is a morphologically well recognizable nuclear organelle and the argyrophilic NORs (AgNORs) are nucleic acid-argyrophilic nonhistone protein complex in the nucleoli and the silver staining allows their identification and enumeration at the light microscopic level. The AgNOR counts are in parallel with mitotic activity and vary in different phase of cell cycle. It has been reported that human cells have one AgNOR during interphase and S-phase. However, the correlation between the number of AgNORs and S-phase markers is still controversial and they have never been studied simultaneously. In this study, AgNOR and PCNA were stained simultaneously to find out the relationship of AgNOR counts with cell cycle (S-phase) in human palatine tonsil, gastric carcinoma, liver and brain tissues. S-phase cells (PCNA-positive) were found predominantly in lymphoid follicles in palatine tonsil but gastric carcinoma showed diffuse immunoreactivity for PCNA. The AgNOR counts varied according to the type and locus of tissue. More than one AgNOR were identified in S-phase cells and some of hepatocytes and neurons in the brain which were not in S-phase contained two or more AgNORs. The above results suggest that the number of AgNOR is a characteristic feature of each type of cells and can be more than one even in S-phase.
Brain ; Cell Cycle ; Hepatocytes ; Humans* ; Interphase ; Liver ; Neurons ; Organelles ; Palatine Tonsil ; Proliferating Cell Nuclear Antigen ; Silver Staining

No abstract available.
Amenorrhea* ; Cytogenetics* ; Female

No abstract available.
Amenorrhea* ; Cytogenetics* ; Female

No abstract available.
Mandibular Condyle* ; Rabbits*

Nodular pseudosarcornatous fasciitis is a benign, fibrohlastic proliferative disease. This case report is one of a hard nodule which a 35-year-old male suddenly discovered on his right forearm 10 days prior to being aeen. The nodule showed characteristic histological findings of nodular pseudosarcomatous fasciitis.
Adult ; Fasciitis* ; Forearm ; Humans ; Male

No abstract available.
Humans ; Lung Neoplasms* ; Lung* ; Survival Analysis*

Generalized lipodyst,rophy is characterized by generalized loss of body fat, and is asociated vith metabolic ahnormalities, including insulin resistance, hyperglycemia., and hypertriglyceridemia. like acanthosis nigricans, generalized lipodystrophy is a cutaneous marker of insulin re.istant diabetes. We report. herein a twenty year old female witti both classic generalized lipodystripin and acanthosis nigricans, in association with insulin resistant diabetes.
Acanthosis Nigricans ; Adipose Tissue ; Female ; Humans ; Hyperglycemia ; Hypertriglyceridemia ; Insulin ; Insulin Resistance ; Lipodystrophy, Congenital Generalized*

No abstract available.
Poisoning*

BACKGROUND: Paraquat, a widely used herbicide, is extremely toxic, causing multiple organ failure in humans. Paraquat especially leads to irreversible progressive pulmonary fibrosis, which is related to oxygen free radicals. However, its biochemical mechanism is not clear. Natural mechanisms that prevent damage from oxygen free radicals include changes in glutathione level, G6PDH, superoxide dismutase(SOD), catalase, and glutathione peroxidase. The authors think catalase is closely related to paraquat toxicity in the lungs METHOD: The effects of 3-amino-1,2,4-triazole(aminotriazole), a catalase inhibitor, on mice administered with paraquat were investigated. We studied the effects of aminotriazole on the survival of mice administered with paraquat, by comparing life spans between the group to which paraquat had been administered and the group to which a combination of paraquat and aminotriazole had been administered. We measured glutathion level, glucose 6-phosphate dehydrogenase(G6PDH), superoxide dismutase(SOD), catalase, and glutathione peroxidase(GPx) in the lung tissue of 4 groups of mice: the control grouts, group A(aminouiazole injected), group B(paraquat administered), group C(Paraquat and aminotriazole administered). RESULTS: The mortality of mice administered with paraquat which were treated with aminotriazole was significantly increased compared with those of mice not treated with aminotriazole. Glutathione level in group B was decreased by 20%, a significant decrease compared with the control group. However, this level was not changed by the administration of aminotriazole(group C). The activity of G6PDH in all groups was not significantly changed compared with the control group. The activities of SOD, catalase, and glutathione peroxidase(GPx) in the lung tissue were significantly decreased by paraquat administration(group B); catalase showed the largest decrease. Catalase and GPX were significantly decreased by aminotriazole treatment in mice administered with paraquat but change in SOD activity was not significant.(group C). CONCLUSION: Decrease in catalase activity by paraquat suggests that paraquat toxicity in the lungs is closely related to catalase activity. Paraquat toxicity in mice is enhanced by aminotriazole administration, and its result is related to the decrease of catalase activity rather than glutathione level in the lungs. Production of hydroxyl radicals, the most reactive oxygen metabolite, is accelerated due to increased hydrogen peroxide by catalase inhibition and the lung damage probably results from nonspecific tissue injury of hydroxyl radicals.
Amitrole* ; Animals ; Catalase ; Free Radicals ; Glucose ; Glutathione ; Glutathione Peroxidase ; Humans ; Hydrogen Peroxide ; Lung* ; Mice* ; Mortality ; Multiple Organ Failure ; Oxygen ; Paraquat* ; Pulmonary Fibrosis ; Superoxides

A Statistical analysis was made on 275 cases of urinary calculi During the period from Jan.. 1963 to Aug. 1972, on the Department of Urology of Han-il Hospital and the results were obtained as following; 1. The incidence of urinary calculi was 3.09 % of out-patients. 2. The age of the patients ranged from 20 to 30 years in approximately 70 percent. 3. A seasonal occurrence was highest during summer on 31 percent. 4. The locational distribution of urinary calculi was 71.6 percent in ureter, 18.2 percent in kidney, 7.5 percent in bladder, 2. 7 percent in urethra. 5. The locational distribution of ureter calculi was 66 percent in lower, 22 percent in middle and 12 percent in upper portion. 6. In a number of urinary calculi, 89. 8 percent was single and 5.8 percent was double and 4.4 % was more than 2. 7. The ureterolithotomy was performed on 74 of 197cases requiring surgical intervention. Cystoscopic manipulation (Spontaneous Expelled) and conservative treatment were 120 cases of 197 cases. Other manner on 3 cases. 8. The results of qualitative analysis of 240 urinary calculi showed cal. Phosphate + oxalate in 53.7 percent, Cal phosphate in 13.7 percent, Calcium phosphate + ammonate in 7.5 percent, Ca phosphate +carbonate in 7.1 percent, Ca carbonate in 5.O percent. Ca oxalate in' 4.2 percent. etc.
Calcium ; Calculi ; Carbon ; Humans ; Incidence ; Kidney ; Outpatients ; Seasons ; Statistics as Topic* ; Ureter ; Urethra ; Urinary Bladder ; Urinary Calculi ; Urolithiasis* ; Urology