1.Study on the Development of a Rapid Detection Method for Pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis by Nested Polymerase Chain Reaction.
Ho Jung OH ; Hong Ki MIN ; Yeo Won SOHN ; Seung Hwa HONG
Journal of the Korean Society for Microbiology 1999;34(2):175-187
A nested polymerase chain reaction (PCR) was applied to detect and identify pathogenic Yersinia enterocolitica and Y. pseudotuberculosis. We used photochemical postamplification procedure with 8-methoxypsoralen to control carryover contamination. Using the ail and inv gene, the sensitivity and specificity of DNA amplification by nested PCR was considerably improved. The amplified fragment sizes were 298 bp for the ail gene and 295 bp for the inv gene. Amplification was successful when the template was derived from three sources: purified DNA, aliquots of boiled bacterial suspension and aliquots of lysed bacterial suspension. The detection limits were 10 fg of DNA and 2 * 10 colony forming units (CFU) for Y. enterocolitica and 10 fg DNA and 2 CFU for Y. pseudotuberculosis.
DNA
;
Limit of Detection
;
Methoxsalen
;
Polymerase Chain Reaction*
;
Sensitivity and Specificity
;
Stem Cells
;
Yersinia enterocolitica*
;
Yersinia pseudotuberculosis*
;
Yersinia*
2.A case of hypodipsic hypernatremia.
Seung Hee PARK ; Hong Sin JEON ; Sun Hwa KIM ; Don Hee AHN
Journal of the Korean Pediatric Society 1993;36(11):1621-1625
Hypodipsic hypernatremia is characterized by chronic or recurrent episodes of severe hypernatermia associated with dehydration and a lack of thirst. This constellation of deficits suggests that the syndrome is due to hypodipsia of destruction of the hypothalamic osmoreceptors that regulate thirst and ADH secretion. We report a child with abnormalities of the central nervous system who had hypernatremia and a lack of thirst without detectable abnormalities in the osmoregulation of ADH secretion. The patient was a 11 month old female and her chief complaints were poor oral intake and weight gain. There were recurrent hypernatremia with hyperosmosis and normal level of plasma ADH. With intravenous rehydratin, oral intake was improved and plasma sodium level decreased.
Central Nervous System
;
Child
;
Dehydration
;
Female
;
Humans
;
Hypernatremia*
;
Infant
;
Osmoregulation
;
Plasma
;
Sodium
;
Thirst
;
Weight Gain
4.Safety and Efficacy of Transluminal Balloon Angioplasty Using a Compliant Balloon for Severe Cerebral Vasospasm after an Aneurysmal Subarachnoid Hemorrhage.
Beom Jin CHOI ; Tae Hong LEE ; Jae Il LEE ; Jun Kyeung KO ; Hwa Seung PARK ; Chang Hwa CHOI
Journal of Korean Neurosurgical Society 2011;49(3):157-162
OBJECTIVE: Vasospasm of cerebral vessels remains a major source of morbidity and mortality after an aneurysmal subarachnoid hemorrhage (SAH). The purpose of this study was to evaluate the safety and efficacy of transluminal balloon angioplasty (TBA) for SAH-induced vasospasm. METHODS: Eleven patients with an angiographically confirmed significant vasospasm (>50% vessel narrowing and clinical deterioration) were studied. A total of 54 vessel segments with significant vasospasm were treated by TBA. Digital subtraction angiography was used to confirm the presence of vasospasm, and TBA was performed to dilate vasospastic arteries. Medical and angiographic reports were reviewed to determine technical efficacy and for procedural complications. RESULTS: TBA using Hyper-Glide or Hyper-Form balloons (MicroTherapeutics, Irvine, CA) was successfully accomplished in 88.9% vasospastic segments (48 of 54), namely, in the distal internal carotid artery (100%, n=7), the middle cerebral artery (100%), including the M1 (n=10), M2 (n=10), and M3 segments (n=4), in the vertebral artery (100%, n=2), basilar artery (100%, n=1), and in the anterior cerebral artery (ACA), including the A1 (66%), A2 (66%), and A3 segments (100%). Vessel diameters significantly increased after TBA. There were no cases of vessel rupture or thromboembolic complications. GCS at one day after TBA showed an improvement in all patients except one. CONCLUSION: This study suggests that TBA using Hyper-Glide or Hyper-Form balloons is a safe and effective treatment for subarachnoid hemorrhage-induced cerebral vasospasm.
Aneurysm
;
Angiography, Digital Subtraction
;
Angioplasty, Balloon
;
Anterior Cerebral Artery
;
Arteries
;
Basilar Artery
;
Carotid Artery, Internal
;
Endovascular Procedures
;
Glycosaminoglycans
;
Humans
;
Middle Cerebral Artery
;
Rupture
;
Subarachnoid Hemorrhage
;
Vasospasm, Intracranial
;
Vertebral Artery
5.Ovulation Induction.
Chung Hoon KIM ; Seung Hwa HONG
Journal of Korean Society of Endocrinology 2002;17(6):841-862
No abstract available.
Female
;
Ovulation Induction*
;
Ovulation*
6.Nd-YAG laser and CO2 laser application in general surgery.
Bong Hwa LEE ; Seung Won JUNG ; Kyung Sik KIM ; Ze Hong WON ; Heung Kil PARK ; Chan Young LEE
Journal of the Korean Surgical Society 1991;40(1):1-12
No abstract available.
Lasers, Gas*
;
Lasers, Solid-State*
7.Clinical Manifestations of Invasive Infections due to Streptococcus pyogenes in Children.
Nuri YANG ; Hyeon Seung LEE ; Jae Hong CHOI ; Eun Young CHO ; Eun Hwa CHOI ; Hoan Jong LEE ; Hyunju LEE
Korean Journal of Pediatric Infectious Diseases 2014;21(2):129-138
PURPOSE: Streptococcus pyogenes is an important cause of invasive diseases in children. We aimed to describe the clinical characteristics of invasive infections due to S. pyogenes in children in Korea. METHODS: A retrospective study of children under 18 years of age with invasive infections due to S. pyogenes at Seoul National University Children's Hospital between March 1992 and December 2012, and Seoul National University Bundang Hospital between March 2003 and December 2012 was conducted. Demographic factors, clinical characteristics, laboratory findings, treatment, mortality and morbidity of all patients were reviewed. RESULTS: A total of 30 among 36 cases identified as invasive disease due to S. pyogenes were available for review. There was a predominance for male subjects (male:female=2.75:1). The median age was 50 months (range 12 days to 15 years) and 53.3% were under 5 years of age. Skin and soft tissue infections (9/30, 30.0%), bacteremia without identified focus (4/30, 13.3%) and bone and joint infections (6/30, 20.0%) were the most frequent clinical presentations. Streptococcal toxic shock syndrome (3/30, 10.0%) pulmonary, abdomen and central nervous system infections (2/30, 6.7%) were also seen. There was a peak in number of patients in year 2012 (9/30, 30.0%). There were no cases of mortality. Erythromycin and clindamycin resistance rates were low by 3.8% and 7.5%, respectively. CONCLUSION: We studied the clinical presentations of invasive infections due to S. pyogenes during the past 20 years in Korean children. The findings of this study help us understand the characteristics of the disease, enhancing early recognition and prompting adequate antibiotic therapy which is important in reducing morbidity and mortality.
Abdomen
;
Bacteremia
;
Bacterial Infections
;
Central Nervous System Infections
;
Child*
;
Clindamycin
;
Demography
;
Erythromycin
;
Humans
;
Joints
;
Korea
;
Male
;
Mortality
;
Retrospective Studies
;
Seoul
;
Shock, Septic
;
Skin
;
Soft Tissue Infections
;
Streptococcus pyogenes*
8.The Diagnostic Value of Isocapnic Hyperventilation of Cold Air in Adults with Suspected Asthma.
Jae Hwa CHO ; Jwong Swon RYU ; Ji Young LEE ; Seung Min KWAK ; Hong Lyeol LEE ; Chul Ho CHO
Tuberculosis and Respiratory Diseases 2001;51(3):232-239
BACKGROUND: Asthmatic patients frequently suffer cold-weather-associated respiratory symptoms. The sensitivity, specificity, accuracy and diagnostic value of isocapnic hyperventilation of cold air(IHCA) using a multistep method was investigated in patients suspected to have asthma. METHOD: One hundred and 29 adult patients who had an IHCA performed between july 1999 and December 2000, had an methacholine bronchoprovocation test because of a clinical suspicion of asthma. RESULTS: According to strict criteria, 50 were defined as asthmatics and 79 as symptomatic nonashmatics. There were no differences in age, sex and smoking state between the asthmatic and symptomatic nonasthmatic groups. There was a significant decrease in the percentage reduction in the forced expiratory volume in 1 second(FEV1) after the IHCA between the asthmatics(-10.0±6.8%) and the symptomatic nonasthmatics(-2.3±2.5%). The factors associated with a reactivity to IHCA were FEV1/FVC, FEF25-75/FVC and FEV1(% of predicted). The accuracy was highest using a 7% fall in FEV1; the sensitivity was 76% and the specificity 96%. CONCLUSION: IHCA is a specific, although not a sensitive, test for diagnosing asthma in adult patients. Furthermore, the diagnostic cut-off value of the different methods of IHCA need to be determined.
Adult*
;
Asthma*
;
Forced Expiratory Volume
;
Humans
;
Hyperventilation*
;
Methacholine Chloride
;
Sensitivity and Specificity
;
Smoke
;
Smoking
9.A Case of Herpes Zoster Oticus Involving Glossopharyngeal Nerve without Facial Nerve Palsy.
Seung Hyun SOHNG ; Jin Hwa CHOI ; Hyo Jin LEE ; Dong Hoon SHIN ; Jong Soo CHOI ; Ki Hong KIM
Korean Journal of Dermatology 2012;50(7):656-657
No abstract available.
Deglutition Disorders
;
Facial Nerve
;
Glossopharyngeal Nerve
;
Herpes Zoster
;
Herpes Zoster Oticus
;
Paralysis
10.A Case of Combined Ectopic Gestation with Cervical and Tubal Components.
Seung Hwa HONG ; Hye Eun KWON ; Sung Hoon KIM ; Hee Dong CHAE ; Chung Hoon KIM ; Byung Moon KANG
Korean Journal of Fertility and Sterility 2003;30(3):249-254
Ectopic pregnancy is a common medical problem that is difficult to diagnose and potentially may lead to significant mortality or morbidity. The incidence of ectopic pregnancy is definitely increasing due to the rise in pelvic inflammatory disease (PID), pelvic surgery, intrauterine device (IUD), and assisted reproductive technologies, such as in vitro fertilization and embryo transfer (IVF-ET). Combined ectopic gestations are much rare and their true incidence is unknown. Multiple ectopic gestations may occur in a variety of locations. The majority involve one or both fallopian tubes. We report a case of combined tubal and cervical pregnancies, and discuss their management.
Embryo Transfer
;
Fallopian Tubes
;
Female
;
Fertilization in Vitro
;
Incidence
;
Intrauterine Devices
;
Mortality
;
Pelvic Inflammatory Disease
;
Pregnancy*
;
Pregnancy, Ectopic
;
Reproductive Techniques, Assisted