No abstract available.
Candidiasis* ; Diabetes Mellitus*

No abstract available.
Peritoneal Dialysis, Continuous Ambulatory* ; Peritonitis*

Chondrodysplasia punctata is a rare congenital disorder of bone, occuring in infants, which is characterized by radiographic manifestation of premature deposition of punctate calcific densitiy in epiphyseal areas, preformed in cartilage. We experienced a case of rhizomelic type-chondrodysplsia punctata in a two day old female who showed short stature, symmetric shortening of proximal limbs, cataract, icthyositic skin lesion and characteristic coronal clefts in lumbar vertebral bodies on X-ray.
Cartilage ; Cataract ; Chondrodysplasia Punctata ; Chondrodysplasia Punctata, Rhizomelic* ; Congenital, Hereditary, and Neonatal Diseases and Abnormalities ; Extremities ; Female ; Humans ; Infant ; Skin

No abstract available.
Catheterization* ; Catheters* ; Edema* ; Subclavian Vein* ; Upper Extremity*

No abstract available.
Diagnosis* ; Growth Hormone* ; Humans*

A small but significant rise in serum potassium level following succinylcholine administration has been observed in normal anesthetized patients. A dangerous increase in serum potassium concentrations after the administration of succinylcholine chloride has been reported in an increasing number of clinical conditions including burns, massive muscle trauma, tetanus and uremia. The hyperkalemia can be of sufficient degree to cause ventricular tachycardia and fibrillation. Serum electrolytes levels were measured in 40 healthy children during induction of anesthesia. They were less than 10 years old and were divided into 4 groups of 10 children each. Group l: Ketamine 2 mg/kg and succinylcholine 2 mg/kg Group llI: Thiopental 5 mg/kg and succinylcholine 2 mg/kg Group lll: Pretreatment with pancuronium 20 ug/kg followed in 3 min by ketamine 2 mg/kg and succinylcholine 2 mg/kg Group lV: Pretreatment with pancuronium 20 ug/kg followed in 3 min. by thiopental 5 mg/kg and succinylcholine 2 mg/kg. Venous blood samples for serum electrolyte estimation were obtained using a catheter placed in an antecubital vein. The following results were obtained: 1) Serum potassium concentration following administration of succinylcholine was significantly increased in group l (p<0.05), but not in group ll, lll, lV. 2) Peak changes of serum potassium concentration were much higher in group l than group ll and lll, and the peak change in group lV was decreased below control values. Variance analysis indicate a significant difference between group I and the others(pp<0.05). 3) The peak change in serum potassium concentration in group I was from 1 min. to 3 min. after succinylcholine. 4) Serum Na+, Cl- and CO2, content were not significantly changed after succinylcholine and statistically not significant in all the groups.
Anesthesia ; Burns ; Catheters ; Child* ; Electrolytes* ; Humans ; Hyperkalemia ; Ketamine* ; Pancuronium* ; Potassium ; Succinylcholine* ; Tachycardia, Ventricular ; Tetanus ; Thiopental* ; Uremia ; Veins

BACKGROUND: The purpose of this study was to measure the magnitude of the hypoxic pulmonary vasoconstriction(HPV) response to hypoxia of left lung and the effect of positive end-expira pressure(PEEP) of right lung on the regional HPV of left lung. METHODS: Left thoracotomy was performed in eight female mongrel dogs. Left pulmonary blood flow(Q) was measured with electromagnetic blood flow probe and cardiac output with tliermodilution technique in triplicate. Systemic and pulmonary hemodynamics were measured via Swan-Ganz and indwelling catheters. Q was shown as percent of cardiac output(Q%). Total, right and left pulmonary v.ascular resistance(PVRt, PVRr and PVR1) and pulmonary shunt(Qg/Qt) were calculated. Through the study, the right lung was ventilated continuously with 100% O2, while the left lung was either ventilated with 100% O2(control: phased 1)and a gas mixture containing 4% O2, 3% CO2 and 93% N2 (hypoxic: phase2I and 3). In phase 3 10 cmH2O PEET was applied to the light lung. RESULTS: Left lung hypoxia in phase 2 results in a reduction of Ql% and PaO2 and a elevation of PVRl without any changes of PVRt, Qs/Qt, MPAP and pulmonary perfusion pressure(PPP). With a 10 cm H2O PEEP on the light lung in phase 3, Ql% returned to the control level, but PVRl was still higher as compared to control . PaO2 and Qs/Qt were further aggravated. PVRt and PVRr were elevated in phase 3 as compared to phase 1and 2. CONCLUSION: It is concluded that contra-lateral PEET during unilateral HPV may aggravate systemic hypoxemia via blood flow diversion away from the PEETed area to the hypoxic area, but not abolish hypoxic pressor respone of hypoxic area.
Animals ; Anoxia ; Cardiac Output ; Catheters, Indwelling ; Dogs ; Female ; Hemodynamics ; Humans ; Lung ; Magnets ; Perfusion ; Positive-Pressure Respiration ; Thoracotomy ; Ventilation

Cytomegalovirus (CMV) is a ubiquitous virus and its infections occur commonly after renal transplantation and immunosuppressive therapy. Early and accurate laboratory diagnosis of CMV infection in renal transplant is necessary but often difficult. To find optimal diagnostic methods for CMV infection, we compared shell vial culture and polymerase chain reaction (PCR) and Southern blot of PCR products. A total of 301 specimens of urine, blood neutrophils, tissues, or body fluids were obtained from 75 renal transplant recipients and were submitted to shell vial culture for CMV as well as DNA PCR using primers for immediate early(IE) gene of CMV. The human fibroblast cell line (MRC-5) was used to culture CMV and were examined with immunofluorescence staining using monoclonal antibody to the early antigen of CMV. The PCR products (274 and 379 bp) were detected by gel electrophoresis and ethidium bromide staining. When PCR products were not clearly visible on electrophoresis, PCR products were analyzed by Southern blot using IE gene probe. Sixty four(85.3%) of 75 renal transplant recipients showed CMV infection as analyzed by PCR and Southern blot as well as shell vial culture. On shell vial culture, CMV were detected in 81 specimens from 30(40%) renal transplant recipients in viremic state. On PCR and Southern blot analysis CMV were detected in 55 and 26 specimens, respectively from 59 patients. The sensitivity of culture and PCR to detect CMV infection were 42.4% and 83.3%, respectively. The results of two studies were concordant in 48%. PCR and Southern blot did not detect CMV in 10 and 5 culture proven CMV positive samples, respectively. Mutant CMV were found in 3 patients which showed 5-10 bp deletion in IE gene. Moreover, DNA sequencing analysis showed 5 mutant strains among 11 strains which appeared same by PCR prodcut. These results suggest that PCR followed by Southern blot may be more sensitive, but less specific than shell vial culture in the diagnosis of CMV disease. PCR followed by Southern blot may not detect mutant CMV. Combined analysis using both shell vial culture and PCR followed by Southern blot may be necessary to diagnose CMV infection in renal transplant recipients.
Blotting, Southern ; Body Fluids ; Cell Line ; Clinical Laboratory Techniques ; Cytomegalovirus Infections* ; Cytomegalovirus* ; Diagnosis ; DNA* ; Electrophoresis ; Ethidium ; Fibroblasts ; Fluorescent Antibody Technique ; Humans ; Kidney Transplantation ; Neutrophils ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Transplantation*

No abstract available.
Histamine* ; Pruritus*

No abstract available.
Arthrogryposis* ; Pyloric Stenosis*