1.Circulating cell-free DNA as a promising biomarker in patients with gastric cancer: diagnostic validity and significant reduction of cfDNA after surgical resection.
Kyongchol KIM ; Dong Gue SHIN ; Min Koo PARK ; Seung Hyuk BAIK ; Tae Hee KIM ; Sanghee KIM ; SaeYoung LEE
Annals of Surgical Treatment and Research 2014;86(3):136-142
PURPOSE: The aim of this study is to determine whether levels of circulating free DNA (cfDNA) increase according to cancer progression, whether they are restored after surgical resection, and to evaluate cfDNA in gastric cancer patients as a useful biomarker. METHODS: A case-control study design was used. Thirty gastric cancer patients and 34 healthy subjects were enrolled from two hospitals in South Korea. The plasma cfDNA of patients with gastric cancer were obtained before surgery and 24 hours after surgery, and then analyzed by a quantitative, real-time polymerase chain reaction. Plasma samples were also obtained from the control group. RESULTS: The mean levels of cfDNA in the healthy control group, patients with early gastric cancer, and with advanced gastric cancer were 79.78 +/- 8.12 ng/mL, 106.88 +/- 12.40 ng/mL, and 120.23 +/- 10.08 ng/mL, respectively (P < 0.01). Sensitivity was 96.67% and specificity was 94.11% when the cutoff value was 90 ng/mL. Variables representing the tumor burden such as tumor size, T stage, TNM stage, and curative resection are also associated with the levels of cfDNA. The levels of cfDNA in the 24-hour-after-surgery group decreased significantly (112.17 +/- 13.42 ng/mL vs. 77.93 +/- 5.94 ng/mL, P < 0.001) compared to the levels of cfDNA in the preoperation group. CONCLUSION: The changes in the levels of cfDNA can act as reliable biomarkers to detect cancer early, to predict tumor burden, estimate curative resection and even prognosis.
Biomarkers
;
Case-Control Studies
;
DNA*
;
Humans
;
Plasma
;
Prognosis
;
Real-Time Polymerase Chain Reaction
;
Republic of Korea
;
Sensitivity and Specificity
;
Stomach Neoplasms*
;
Tumor Burden
2.Differentiation of Osteoblast Progenitor Cells from Human Umbilical Cord Blood.
Seung Jin HONG ; Eun A LEE ; Gue Tae CHAE ; Hoon HAN
Immune Network 2002;2(3):166-174
BACKGROUND: Human umbilical cord bloods, which could be taken during the delivery are utilized as a source of hematopoietic stem cells. Also in cord blood, there are several kinds of stem cells such as endothelial and mesenchymal stem cells. METHODS: We isolated the mesenchymal stem cells from human umbilical cord bloods and confirmed the differentiation of these cells into osteoblast progenitor cells. The mesenchymal stem cells derived from umbilical cord blood have the ability to differentiate into specific tissue cells, which is one of characteristics of stem cells. These cells were originated from the multipolar shaped cells out of adherent cells of the umbilical cord blood mononuclear cell culture. RESULTS: The mesenchymal stem cells expressed cell surface antigen CD13, CD90, CD102, CD105, alpha-smooth muscle actin and cytoplasmic antigen vimentine. Having cultrued these cells in bone formation media, we observed the formation of extracellular matrix and the expression of alkaline phosphatase and of mRNA of cbfa-1, ostoecalcin and type I collagen. CONCLUSION: From these results we concluded that the cells isolated from the umbilical cord blood were mesenchymal stem cells, which we could differentiate into osteoblast when cultured in bone formation media. In short, it is suggested that these cells could be used as a new source of stem cells, which has the probability to alternate the embryonic stem cells.
Actins
;
Alkaline Phosphatase
;
Antigens, Surface
;
Cell Culture Techniques
;
Collagen Type I
;
Cytoplasm
;
Embryonic Stem Cells
;
Extracellular Matrix
;
Fetal Blood*
;
Hematopoietic Stem Cells
;
Humans*
;
Mesenchymal Stromal Cells
;
Osteoblasts*
;
Osteogenesis
;
RNA, Messenger
;
Stem Cells*
;
Umbilical Cord*
;
Vimentin
3.Differentiation of Osteoblast Progenitor Cells from Human Umbilical Cord Blood.
Seung Jin HONG ; Eun A LEE ; Gue Tae CHAE ; Hoon HAN
Immune Network 2002;2(3):166-174
BACKGROUND: Human umbilical cord bloods, which could be taken during the delivery are utilized as a source of hematopoietic stem cells. Also in cord blood, there are several kinds of stem cells such as endothelial and mesenchymal stem cells. METHODS: We isolated the mesenchymal stem cells from human umbilical cord bloods and confirmed the differentiation of these cells into osteoblast progenitor cells. The mesenchymal stem cells derived from umbilical cord blood have the ability to differentiate into specific tissue cells, which is one of characteristics of stem cells. These cells were originated from the multipolar shaped cells out of adherent cells of the umbilical cord blood mononuclear cell culture. RESULTS: The mesenchymal stem cells expressed cell surface antigen CD13, CD90, CD102, CD105, alpha-smooth muscle actin and cytoplasmic antigen vimentine. Having cultrued these cells in bone formation media, we observed the formation of extracellular matrix and the expression of alkaline phosphatase and of mRNA of cbfa-1, ostoecalcin and type I collagen. CONCLUSION: From these results we concluded that the cells isolated from the umbilical cord blood were mesenchymal stem cells, which we could differentiate into osteoblast when cultured in bone formation media. In short, it is suggested that these cells could be used as a new source of stem cells, which has the probability to alternate the embryonic stem cells.
Actins
;
Alkaline Phosphatase
;
Antigens, Surface
;
Cell Culture Techniques
;
Collagen Type I
;
Cytoplasm
;
Embryonic Stem Cells
;
Extracellular Matrix
;
Fetal Blood*
;
Hematopoietic Stem Cells
;
Humans*
;
Mesenchymal Stromal Cells
;
Osteoblasts*
;
Osteogenesis
;
RNA, Messenger
;
Stem Cells*
;
Umbilical Cord*
;
Vimentin
4.Efficacy of Spinal Implant Removal After Thoracolumbar Junction Fusion.
Seok Won KIM ; Chang Il JU ; Chong Gue KIM ; Seung Myung LEE ; Ho SHIN
Journal of Korean Neurosurgical Society 2008;43(3):139-142
OBJECTIVE: The purpose of this study was to evaluate the efficacy of spinal implant removal and to determine the possible mechanisms of pain relief. METHODS: Fourteen patients with an average of 42 years (from 22 to 67 years) were retrospectively evaluated. All patients had posterior spinal instrumentation and fusion, who later developed recurrent back pain or persistent back pain despite a solid fusion mass. Patients' clinical charts, operative notes, and preoperative x-rays were evaluated. Relief of pain was evaluated by the Visual Analog Scale (VAS) pain change after implant removal. Clinical outcome using VAS and modified MacNab's criteria was assessed on before implant removal, 1 month after implant removal and at the last clinical follow-up. Radiological analysis of sagittal alignment was also assessed. RESULTS: Average follow-up period was 18 months (from 12 to 25 months). There were 4 patients who had persistent back pain at the surgical site and 10 patients who had recurrent back pain. The median time after the first fusion operation and the recurrence of pain was 6.5 months (from 3 to 13 months). All patients except one had palpation pain at operative site. The mean blood loss was less than 100ml and there were no major complications. The mean pain score before screw removal and at final follow up was 6.4 and 2.9, respectively (p<0.005). Thirteen of the 14 patients were graded as excellent and good according to modified MacNab's criteria. Overall 5.9 degrees of sagittal correction loss was observed at final follow up, but was not statistically significant. CONCLUSION: For the patients with persistent or recurrent back pain after spinal instrumentation, removal of the spinal implant may be safe and an efficient procedure for carefully selected patients who have palpation pain and are unresponsive to conservative treatment.
Back Pain
;
Follow-Up Studies
;
Humans
;
Palpation
;
Recurrence
;
Retrospective Studies
5.Anterior Interbody Fusion through the Retropharyngeal Approach for Hangman's Fracture: Report of 2 Cases.
Pan Seok JEON ; Seung Myung LEE ; Jin Gue SONG ; Suk Jung JANG ; Ho SHIN
Journal of Korean Neurosurgical Society 1995;24(4):458-463
Most patients with hangman's fracture frequently responded to consertvative therapy consisted of skeletal traction and halo vest, and surgery to stabilize or reduce further injury is seldom necessary. But occasionally operative stabilization may be necessary and can be achiveed satisfactorilly through anterior or posterior approach. Anterior interbody fusion at the G2-3 interspace has advantages of immediate bony stabilization with preservation of rotatory motion compared with posterior approach.
Humans
;
Traction
6.A Case of Aconite Intoxication and Recurrent Ventricular Arrhythmia without Apparent Myocardial Damage after 20,680 Joules DC Shock.
Young Ju JIN ; Ji Hyun LEE ; Jae Hong CHOI ; Byoung Gue NA ; Gi Byoung NAM ; Dong Woon KIM ; Jae Ho EARM ; Myeong Chan CHO ; Seung Taik KIM
Korean Circulation Journal 1997;27(7):780-786
The aconite root has been used in oriental medicine to improve metabolism of debilitated patient and to cure acute dysuria, cardiac weakness, gout, neuralgias and rheumatism. The crude drug "bu-shi" or "cho-oh", which is obtained from the Aconitum roots, contains the potent poisons aconitine, mesaconitine, jesaconitine, and hypaconitine, which are C
Aconitine
;
Aconitum*
;
Adult
;
Alkaloids
;
Animal Experimentation
;
Arrhythmias, Cardiac*
;
Dizziness
;
Dysuria
;
Gout
;
Hemodynamics
;
Humans
;
Medicine, East Asian Traditional
;
Metabolism
;
Nausea
;
Neuralgia
;
Poisons
;
Rheumatic Diseases
;
Shock*
;
Tachycardia
;
Tachycardia, Ventricular
;
Torsades de Pointes
;
Ventricular Fibrillation
;
Vomiting
7.Minimally Invasive Lumbar Spinal Decompression: A Comparative Study Between Bilateral Laminotomy and Unilateral Laminotomy for Bilateral Decompression.
Seok Won KIM ; Chang Il JU ; Chong Gue KIM ; Seung Myung LEE ; Ho SHIN
Journal of Korean Neurosurgical Society 2007;42(3):195-199
OBJECTIVE: Bilateral laminotomy and unilateral laminotomy for bilateral decompression are becoming the minimally invasive procedures for lumbar spinal stenosis (LSS). With the aim of less invasiveness and better preservation of spinal stability, these techniques have been developed. But there are no large randomized studies to show the surgical results between these two techniques. The objective of this study was to examine the safety and efficacy of these two minimally invasive techniques. METHODS: A total of 80 patients were included in this study (Group I : bilateral laminotomy, Group II : Unilateral laminotomy for bilateral decompression). Perioperative parameters and complications were analyzed. Symptoms and scores such as visual analog scale (VAS) scores, Oswestry Disability Index (ODI) scores, and SF-36 scores of prospectively accrued patients were assessed preoperatively and at 1 month and 12 months after surgery. Paired-t test, two-sample student-t tests, and nonparametric tests were used to determine cross-sectional differences between two groups. RESULTS: No major complications such as spinal instability or deaths occurred during follow-up periods. VAS, ODI scores and SF-36 body pain and physical function scores showed statistically significant improvements in both groups (p<0.001). The significant widening of the spinal canal diameter was also noted in both groups. But, in Group II, there were minor postoperative complications such as dural tear (2 cases 5.0%), fracture of ipsilateral inferior facet (1 case 2.5%), and 5 cases of transient leg symptoms of contralateral side. CONCLUSION: Both bilateral laminotomy and unilateral laminotomy for bilateral decompression allow achievement of adequate and long-lasting operative results in patients with LSS. But postoperative complications are more frequent in Group II (unilateral laminotomy and bilateral decompression). These results indicate that bilateral laminotomy is the preferred minimally invasive technique to treat symptomatic LSS.
Decompression*
;
Follow-Up Studies
;
Humans
;
Laminectomy*
;
Leg
;
Postoperative Complications
;
Prospective Studies
;
Spinal Canal
;
Spinal Stenosis
;
Surgical Procedures, Minimally Invasive
;
Visual Analog Scale
8.Minimally Invasive Lumbar Spinal Decompression: A Comparative Study Between Bilateral Laminotomy and Unilateral Laminotomy for Bilateral Decompression.
Seok Won KIM ; Chang Il JU ; Chong Gue KIM ; Seung Myung LEE ; Ho SHIN
Journal of Korean Neurosurgical Society 2007;42(3):195-199
OBJECTIVE: Bilateral laminotomy and unilateral laminotomy for bilateral decompression are becoming the minimally invasive procedures for lumbar spinal stenosis (LSS). With the aim of less invasiveness and better preservation of spinal stability, these techniques have been developed. But there are no large randomized studies to show the surgical results between these two techniques. The objective of this study was to examine the safety and efficacy of these two minimally invasive techniques. METHODS: A total of 80 patients were included in this study (Group I : bilateral laminotomy, Group II : Unilateral laminotomy for bilateral decompression). Perioperative parameters and complications were analyzed. Symptoms and scores such as visual analog scale (VAS) scores, Oswestry Disability Index (ODI) scores, and SF-36 scores of prospectively accrued patients were assessed preoperatively and at 1 month and 12 months after surgery. Paired-t test, two-sample student-t tests, and nonparametric tests were used to determine cross-sectional differences between two groups. RESULTS: No major complications such as spinal instability or deaths occurred during follow-up periods. VAS, ODI scores and SF-36 body pain and physical function scores showed statistically significant improvements in both groups (p<0.001). The significant widening of the spinal canal diameter was also noted in both groups. But, in Group II, there were minor postoperative complications such as dural tear (2 cases 5.0%), fracture of ipsilateral inferior facet (1 case 2.5%), and 5 cases of transient leg symptoms of contralateral side. CONCLUSION: Both bilateral laminotomy and unilateral laminotomy for bilateral decompression allow achievement of adequate and long-lasting operative results in patients with LSS. But postoperative complications are more frequent in Group II (unilateral laminotomy and bilateral decompression). These results indicate that bilateral laminotomy is the preferred minimally invasive technique to treat symptomatic LSS.
Decompression*
;
Follow-Up Studies
;
Humans
;
Laminectomy*
;
Leg
;
Postoperative Complications
;
Prospective Studies
;
Spinal Canal
;
Spinal Stenosis
;
Surgical Procedures, Minimally Invasive
;
Visual Analog Scale
9.Safety and Cost-Effectiveness of Bridge Therapies for Invasive Dental Procedures in Patients with Mechanical Heart Valves.
Ki Bum WON ; Seung Hyun LEE ; Hyuk Jae CHANG ; Chi Young SHIM ; Gue Ru HONG ; Jong Won HA ; Namsik CHUNG
Yonsei Medical Journal 2014;55(4):937-943
PURPOSE: Bridge anticoagulation therapy is mostly utilized in patients with mechanical heart valves (MHV) receiving warfarin therapy during invasive dental procedures because of the risk of excessive bleeding related to highly vascular supporting dental structures. Bridge therapy using low molecular weight heparin may be an attractive option for invasive dental procedures; however, its safety and cost-effectiveness compared with unfractionated heparin (UFH) is uncertain. MATERIALS AND METHODS: This study investigated the safety and cost-effectiveness of enoxaparin in comparison to UFH for bridge therapy in 165 consecutive patients (57+/-11 years, 35% men) with MHV who underwent invasive dental procedures. RESULTS: This study included 75 patients treated with UFH-based bridge therapy (45%) and 90 patients treated with enoxaparin-based bridge therapy (55%). The bleeding risk of dental procedures and the incidence of clinical adverse outcomes were not significantly different between the UFH group and the enoxaparin group. However, total medical costs were significantly lower in the enoxaparin group than in the UFH group (p<0.001). After multivariate adjustment, old age (> or =65 years) was significantly associated with an increased risk of total bleeding independent of bridging methods (odds ratio, 2.51; 95% confidence interval, 1.15-5.48; p=0.022). Enoxaparin-based bridge therapy (beta=-0.694, p<0.001) and major bleeding (beta=0.296, p=0.045) were significantly associated with the medical costs within 30 days after dental procedures. CONCLUSION: Considering the benefit of enoxaparin in cost-effectiveness, enoxaparin may be more efficient than UFH for bridge therapy in patients with MHV who required invasive dental procedures.
Aged
;
Anticoagulants/*therapeutic use
;
Dentistry, Operative/*methods
;
Enoxaparin/therapeutic use
;
Female
;
*Heart Valve Prosthesis
;
Heparin, Low-Molecular-Weight/*therapeutic use
;
Humans
;
Male
;
Middle Aged
10.The Efficacy of Modified Puberty Suppression Score by Single Blood Sample for Evaluation of GnRH Agonist Treatment in Central Precocious Puberty.
Seung Gue LEE ; Woo Jin CHOI ; Me Jin KIM ; Young Ho KIM ; Ji Ah JUNG ; Il Tae HWANG ; Seung YANG ; Hae Ran LEE
Journal of Korean Society of Pediatric Endocrinology 2005;10(2):181-187
PURPOSE: GnRH stimulation test is golden standard for the diagnosis of central precocious puberty as well as evaluation of treatment, however, it is more expensive and inconvenient. This is the reason why many other tests have been suggested. We studied the efficacy of modified puberty suppression score by single blood sample for evaluation of GnRH agonist treatment in central precocious puberty. METHODS: Twenty-four girls (age, 9.56+/-1.56 years) diagnosed with early puberty or precocious puberty at Kangdong Sacred Heart Hospital from March 2002 to May 2005 were included in this study. All of patients were treated with leuprorelin acetate (83.66-115.12 microgram/kg). Total 24 patients including 11 suppression and 13 non-suppression cases were analyzed. The serum levels of LH, FSH, estradiol and progesterone were measured before and 8 weeks after treatment. The height, weight, bone age and Tanner stage of breast development in each patient were also measured before and 12 weeks after treatment. We modified puberty suppression score by Mul et al. in 1999. We defined scores based on statistical significance - estradiol, 2 points (>=1.36 ng/dL), progesterone, 2 points (>=0.31 ng/dL), LH, 1 point (>=2.0 IU/L), delta BA/delta CA, 1 point (>=0), delta HtSDS, 1 point (>=0.25/6 mo). Total score is 7 points and we defined suppression is less than 3 points. RESULTS: The serum levels of estradiol (<1.36 ng/dL, P=0.000) and progesterone (<0.31 ng/dL, P= 0.003) are significantly lower in suppression group than nonsuppression group. If the score according to modified puberty suppression score (MPSS) is less than 3 points, which is considered as a successful suppression by GnRH agonist. The sensitivity, specificity, positive predictive value and negative predictive value of MPSS are 100%, 92.8%, 90.9% and 100% respectively. CONCLUSION: Single blood sample is simpler and easier than GnRH stimulation test for the evaluation and monitoring of GnRH agonist treatment in central precocious puberty and MPSS by single blood sample may be useful in outpatient clinic.
Adolescent
;
Ambulatory Care Facilities
;
Breast
;
Diagnosis
;
Estradiol
;
Female
;
Gonadotropin-Releasing Hormone*
;
Heart
;
Humans
;
Leuprolide
;
Progesterone
;
Puberty*
;
Puberty, Precocious*
;
Sensitivity and Specificity