Genomic DNAs were extracted from cervical lavages of 49 patients with cervical cancer. Dot and Southern blot hybridization were performed using the P-labeled HFV DNA probes to find high risk HPV(type 16 and 18) infection that is known as the mast prevalent pathogenic factor in cervical cancer. Furthermore, genornic DNAs purified frnm cervical cancer tissues were studied in 8 out of 49 patients allowing us to convince the results from cervical lavages. The results were as follaws: 1. Dot blot analysis were used to examine the sensitivity and specificity of hybridization condition and HPV-DNA probes. Fasitive signals were obtained even at the level of 10pg for HPV DNA, but no signals could he detected at the level of as much as 400pg for salmon sperm DNA. 2. Dot blot of DNAs from cervircal lavages showed positive signals in 32.7%(16/49) with HPV type 16 probe and 20.4% (10/49)and one mixed infection was found. 3. When the DNAs from cervircal lavages of 49 patients were classified according to the clinical stage of cervical cancer, the infection rates of HPV type 16 and 18 were 50% (2/4) in CIN, 80% (4/5) in stage I, 64. 2% (9/14) in stage I b, 45% (9I20) in stage II and 16. 7% (1/6) in stage Ill and K respectively. The occurrenr,e of HPV type 16 and 18 seemed to be the highest in the cervical cancer stage 1 (68.4%(13/19). 4. Experiments perfornecl with genomic DNAs from 8 cancer tissues showed similar results compared to those of cervical lavages, but the intensity of positive signals was stronger. 5. Genomic DNAs from 5 patients(3 cases from cervical lavages and 2 cases from cervical cancer tissues) which showed strong positive signals to the dot blot analysis were further examined by Southern blot hybridixation using HFV type 16 DNA probe. When DNAs were digested with Pst 1 restriction enzyme, the five characteristic frgmenta of BFV type 16(2.8, l.9, l.6, 1.0 and 0.5 kb long in length) were recognized in ell 5 cases, These results may suggest a direet relatianship between HPV type 16 & 18 infectioas considered as the most effective methods for HPV detectioe and typing. Mo1ecular biclogieal studies in the reserarch of HPV are expected to reveal and help us understand the pathogenesis of cervical cancer.
Blotting, Southern ; Coinfection ; DNA Probes ; DNA* ; Humans* ; Salmon ; Sensitivity and Specificity ; Spermatozoa ; Therapeutic Irrigation ; Uterine Cervical Neoplasms*

Sustainable rates of sebum excretion(SRSE) was measured in seven women who had normal regular periods, none were taking oral contraceptives with no significant signs of acne or hirsutism, with assays for testosterone, dihydrotestosterone, dehydroepiandrosterone sulfate, and progesterone during menstrual cycle. The SRSE in omen during the follicular phase wa lower in the luteal phase (p <0.01)than in the men (p <0.01). However during the luteal phase the SRSE in the women was increased similar to the SESE in the men. We investigated the above horrnoies in relationship to the SRSE during the women's menstrua] cycle. During the follicular phase the hormone which was the most determining factor affected the SRSl was total testerone, while dihydrotestosterone was the most determining factor of the horrnone affecting the SRSE during the ovulation and luteal phase.
Acne Vulgaris ; Contraceptives, Oral ; Dehydroepiandrosterone Sulfate ; Dihydrotestosterone ; Female ; Follicular Phase ; Hirsutism ; Humans ; Luteal Phase ; Male ; Menstrual Cycle* ; Ovulation ; Progesterone ; Sebum* ; Testosterone

Accidents, Traffic ; Early Diagnosis ; Female ; Fractures, Bone ; Humans ; Jeollanam-do ; Lacerations ; Male ; Mandibular Fractures ; Surgery, Oral

The dopamine D4 receptor gene has a hypervariable segment in the coding region charcterized by a varying number of 48bp repeats in exon III of the gene. Varying the numbers of repeated segments may change the length, structure, and function of the receptor, which makes this gene a possible candidate for variations in dopamine-related behaviors. such as alcoholism and drug abuse. We evaluated the dopamine D4 receptor genotype in male alcoholics and normal controls. All alcoholics and controls were unrelated and from the Korean population. Genotype and allele frequencies in 67 alcoholics were compared to 67 controls who were free of alcohol abuse. substance abuse. and major mental illness. No association was found between the dopamin D4 recepto allele and alcoholism. This result indicate that there is no association of the dopamine D4 receptor with alcoholism in Korean. Further systemized investigation to determine the role of dopamine D4 receptor gene in alcoholism with a larger sample size will be required.
Alcoholics ; Alcoholism* ; Alleles ; Clinical Coding ; Dopamine* ; Exons ; Gene Frequency ; Genotype ; Humans ; Male ; Receptors, Dopamine D4* ; Sample Size ; Substance-Related Disorders

No abstract available.
Burns* ; Hand*

No Abstract Available.
Adult* ; Capsulorhexis* ; Cataract* ; Follow-Up Studies* ; Humans

Alcohol has extensive effects on sleep and daytime sleepiness. Alcohol has a sleep inducing effect and the effect of increased non-REM sleep and suppressed REM sleep during the first half portion of night sleep, but alcohol induces the effect of decreased non-REM sleep and increased light sleep and frequent awakenings and REM rebound during the second half portion of night sleep. Alcohol provokes chronobiological change such as the changes of amplitude or the phase shifts of hormones or core body temperature. The sleep disruption resulting from alcohol drinking may lead to daytime fatigue and sleepiness. The elderly are at particular in the increased risk of alcohol-related sleep disorders because they achieve higher levels of alcohol in the blood and brain than do younger adults after consuming an equivalent dose. Bedtime alcohol consumption among older adults may lead to unsteadiness if walking is attempted during the night, with increased risk of falls and injuries. Continued alcohol use for sleep induction often induces aggravation of insomnia, alcoholism or sleep related breathing disorders such as obstructive sleep apnea. Alcohol should not be used as substitution of sleep pill because of the dependence and tolerance for sleep inducing effect, and the sleep disruption produced by alcohol withdrawal.
Adult ; Aged ; Alcohol Drinking ; Alcoholism ; Body Temperature ; Brain ; Fatigue ; Humans ; Respiration ; Sleep Apnea, Obstructive ; Sleep Wake Disorders ; Sleep Initiation and Maintenance Disorders ; Sleep, REM ; Walking

No abstract available.
Cephalosporins* ; Pneumonia* ; Salicylates*

No abstract available.
Femur* ; Phlebography* ; Prognosis*

Age Distribution ; Ampicillin ; Anti-Bacterial Agents ; Bacteria ; Cefotaxime ; Clindamycin ; Erythromycin ; Humans ; Incidence ; Jeollanam-do ; Lacerations ; Maxillofacial Injuries ; Motor Vehicles ; Mouth ; Oxacillin ; Penicillins ; Skin ; Staphylococcus ; Streptococcus ; Surgery, Oral ; Wound Infection ; Wounds and Injuries