Objective To evaluate the chemical composition of the essential oil isolated from Elaeoselinum asclepium (L.) Bertol. (E. asclepium), and test the efficiency of the essential oil as an antimicrobial and antioxidant agent. Methods Essential oil was obtained from the aerial parts of E. asclepium by hydro distillation and analyzed by gas chromatography and gas chromatography coupled with mass spectrometry. We study for the first time the chemical composition of the essential oil of E. asclepium, followed by the in vitro antimicrobial activities, which were evaluated by agar diffusion method against six Gram-positive bacteria, five Gram-negative bacteria, and two fungi. In addition, The antioxidant activities were also investigated using assays of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and ferric-reducing capacity. Results The analyzed essential oil of the aerial parts of E. asclepium was rich in α-pinene (43.9%), other compounds detected in appreciable amounts were sabinene (27.9%) and β-pinene (16.0%). The essential oil yields 1.2%, the IC