1.Carnosic acid inhibits TLR4-MyD88 signaling pathway in LPS-stimulated 3T3-L1 adipocytes.
Mi Young PARK ; Seong Taek MUN
Nutrition Research and Practice 2014;8(5):516-520
BACKGROUND/OBJECTIVES: Carnosic acid (CA), found in rosemary (Rosemarinus officinalis) leaves, is known to exhibit anti-obesity and anti-inflammatory activities. However, whether its anti-inflammatory potency can contribute to the amelioration of obesity has not been elucidated. The aim of the current study was to investigate the effect of CA on Toll-like receptor 4 (TLR4) pathways in the presence of lipopolysaccharide (LPS) in 3T3-L1 adipocytes. MATERIALS/METHODS: 3T3-L1 adipocytes were treated with CA (0-20 microM) for 1 h, followed by treatment with LPS for 30 min; mRNA expression of adipokines and protein expression of TLR4-related molecules were then measured. RESULTS: LPS-stimulated 3T3-L1 adipocytes showed elevated mRNA expression of tumor necrosis factor (TNF)-alpha, interleukin-6, and monocyte chemoattractant protein-1, and CA significantly inhibited the expression of these adipokine genes. LPS-induced up regulation of TLR4, myeloid differentiation factor 88, TNF receptor-associated factor 6, and nuclear factor-kappaB, as well as phosphorylated extracellular receptor-activated kinase were also suppressed by pre-treatment of 3T3-L1 adipocytes with CA. CONCLUSIONS: Results of this study suggest that CA directly inhibits TLR4-MyD88-dependent signaling pathways and decreases the inflammatory response in adipocytes.
Adipocytes*
;
Adipokines
;
Chemokine CCL2
;
Inflammation
;
Interleukin-6
;
Myeloid Differentiation Factor 88
;
Obesity
;
Phosphotransferases
;
RNA, Messenger
;
TNF Receptor-Associated Factor 6
;
Toll-Like Receptor 4
;
Tumor Necrosis Factor-alpha
;
Up-Regulation
2.Dietary carnosic acid suppresses hepatic steatosis formation via regulation of hepatic fatty acid metabolism in high-fat diet-fed mice.
Mi Young PARK ; Seong Taek MUN
Nutrition Research and Practice 2013;7(4):294-301
In this study, we examined the hepatic anti-steatosis activity of carnosic acid (CA), a phenolic compound of rosemary (Rosmarinus officinalis) leaves, as well as its possible mechanism of action, in a high-fat diet (HFD)-fed mice model. Mice were fed a HFD, or a HFD supplemented with 0.01% (w/w) CA or 0.02% (w/w) CA, for a period of 12 weeks, after which changes in body weight, blood lipid profiles, and fatty acid mechanism markers were evaluated. The 0.02% (w/w) CA diet resulted in a marked decline in steatosis grade, as well as in homeostasis model assessment of insulin resistance (HOMA-IR) index values, intraperitoneal glucose tolerance test (IGTT) results, body weight gain, liver weight, and blood lipid levels (P < 0.05). The expression level of hepatic lipogenic genes, such as sterol regulating element binding protein-1c (SREBP-1c), liver-fatty acid binding protein (L-FABP), stearoyl-CoA desaturase 1 (SCD1), and fatty acid synthase (FAS), was significantly lower in mice fed 0.01% (w/w) CA and 0.02% (w/w) CA diets than that in the HFD group; on the other hand, the expression level of beta-oxidation-related genes, such as peroxisome proliferator-activated receptor alpha (PPAR-alpha), carnitine palmitoyltransferase 1 (CPT-1), and acyl-CoA oxidase (ACO), was higher in mice fed a 0.02% (w/w) CA diet, than that in the HFD group (P < 0.05). In addition, the hepatic content of palmitic acid (C16:0), palmitoleic acid (C16:1), and oleic acid (C18:1) was significantly lower in mice fed the 0.02% (w/w) CA diet than that in the HFD group (P < 0.05). These results suggest that orally administered CA suppressed HFD-induced hepatic steatosis and fatty liver-related metabolic disorders through decrease of de novo lipogenesis and fatty acid elongation and increase of fatty acid beta-oxidation in mice.
Acyl Coenzyme A
;
Acyl-CoA Oxidase
;
Animals
;
Body Weight
;
Carnitine O-Palmitoyltransferase
;
Carrier Proteins
;
Diet
;
Diet, High-Fat
;
Diterpenes, Abietane
;
Fatty Acid Synthetase Complex
;
Fatty Acids, Monounsaturated
;
Glucose Tolerance Test
;
Hand
;
Homeostasis
;
Insulin Resistance
;
Lipogenesis
;
Liver
;
Mice
;
Oleic Acid
;
Palmitic Acid
;
Phenol
;
Plant Extracts
;
PPAR alpha
;
Stearoyl-CoA Desaturase
3.Ruptured Tubal Pregnancy with Massive Retroperitoneal Hemorrhage.
Soonchunhyang Medical Science 2017;23(1):61-64
Ectopic pregnancy is an implantation of the fertilized ovum on a place except the endometrium. Most of the ectopic pregnancies are located at the fallopian tube. Few cases of retroperitoneal hematoma associated with ectopic pregnancy have been reported on previously; in each the retroperitoneal space had been the site of implantation. In contrast, we treated a patient with an ectopic pregnancy that implanted in the tube and then perforated through into the retroperitoneal space. To our knowledge this is very rare case of retroperitoneal hematoma caused by a ruptured tubal pregnancy.
Endometrium
;
Fallopian Tubes
;
Female
;
Hematoma
;
Hemorrhage*
;
Humans
;
Laparoscopy
;
Pregnancy
;
Pregnancy, Ectopic
;
Pregnancy, Tubal*
;
Retroperitoneal Space
;
Zygote
4.Primary malignant melanoma arising in a cystic teratoma.
Obstetrics & Gynecology Science 2013;56(3):201-204
The malignant transformation of a cystic teratoma is a rare event, occurring in about 0.2% to 1.8%. Primary malignant melanoma arising from the ovary is extremely rare. A primary melanoma in an ovarian cystic teratoma originates most frequently at the dermoepidermal junction, similar to a cutaneous melanoma. Though there are no standard adjuvant regimens for the treatment of primary malignant melanoma of the ovary. We present another case report of malignant melanoma developing in cystic teratoma.
Female
;
Melanoma
;
Ovarian Cysts
;
Ovary
;
Teratoma
5.Struma Ovarii: A Case of Struma Ovarii and Literature Review.
Aeli RYU ; Seong Taek MUN ; Gaeul MOON ; Si Hyong JANG
Soonchunhyang Medical Science 2014;20(2):191-194
Struma ovarii is a rare, monodermal and highly specialized teratoma, composed entirely or predominantly (>50%) of thyroid tissue. Presenting symptoms are not specific. Despite containing thyroid tissue, only 5% of struma ovarii have features of hyperthyroidism. Therefore, preoperative diagnosis of struma ovarii is difficult. Recently, the authors experienced a case of struma ovarii found in a young woman who presented with known pelvic mass and dysmenorrhea. A transabdominal ultrasonography and computed tomography detected a 16-cm sized multiloculated mass in pelvic cavity. She underwent laparoscopic unilateral ovarian wedge resection. The final histopathologic diagnosis was struma ovarii of the mature cystic teratoma. Therefore, we report this rare case with a brief review of the literature.
Diagnosis
;
Dysmenorrhea
;
Female
;
Humans
;
Hyperthyroidism
;
Struma Ovarii*
;
Teratoma
;
Thyroid Gland
;
Ultrasonography
6.A Case of Mixed Small and Large Cell Neuroendocrine Carcinoma of the Uterine Cervix.
Aeli RYU ; Seong Taek MUN ; Shi Sun KIM
Soonchunhyang Medical Science 2014;20(2):108-111
A rare case of mixed carcinoma of the cervix is reported, composed of a small and large cell neuroendocrine carcinoma. Neuroendocrine cervical carcinomas are very rare and aggressive. The prognosis is very poor despite multimodal treatment. Earlier reports have shown that the majority of patients present with advanced stage disease, have lymph node metastasis, and are at a high risk for recurrence and disease progression. In this study, we report the case of a 26-year-old woman with composite small and large cell neuroendocrine carcinoma of the cervix. A woman presented with vaginal bleed since 2 months of presentation. Gynecologic examination revealed cervical enlargement, and punch biopsy of the cervical lesion was performed. The biopsy was disclosed a large cell neuroendocrine carcinoma. The patient underwent a radical hysterectomy with bilateral salpingo-oophorectomy and pelvic lymph node dissection. The final histopathologic findings are mixed small and large cell neuroendocrine carcinoma of cervix.
Adult
;
Biopsy
;
Carcinoma, Neuroendocrine*
;
Cervix Uteri*
;
Combined Modality Therapy
;
Disease Progression
;
Female
;
Humans
;
Hysterectomy
;
Lymph Node Excision
;
Lymph Nodes
;
Neoplasm Metastasis
;
Prognosis
;
Recurrence
;
Uterine Cervical Neoplasms
7.Acute renal failure associated with ureteral stone of the unilateral kidney and uterus didelphys with hemivaginal obstruction.
Ae Li RYU ; Yun Sook KIM ; Seong Taek MUN ; Seob JEON ; Seung Do CHOI ; Jae Gun SUNWOO ; Dong Han BAE
Korean Journal of Obstetrics and Gynecology 2009;52(2):261-265
The unique clinical syndrome of the uterus didelphys, a unilateral partially or completely obstructed vagina, and ipsilateral renal agenesis is quite rare, and observed on the right side in greater frequency than on the left. The case of fifteen year old girl with uterus didelphys, unilateral partially obstructed vagina and acute renal failure associated with ureteral stone in unilateral kidney is described. Extracorporealshock wave lithotripsy (ESWL) and evacuation of the hematocolpos and excision of the vaginal septum were done. Aware of this relatively rare condition is mainstay of prompt diagnosis which will prevent permanent renal failure and unnecessary and destructive surgery.
Acute Kidney Injury
;
Congenital Abnormalities
;
Female
;
Hematocolpos
;
Kidney
;
Kidney Diseases
;
Lithotripsy
;
Renal Insufficiency
;
Ureter
;
Uterus
;
Vagina
8.A case of laparoscopic treatment of collision tumor: Huge serous cystadenoma and dermoid cyst arising in the same ovary.
Na Hye LEE ; Jung Hui HONG ; Yun Sook KIM ; Seong Taek MUN ; Seob JEON ; Jae Gun SUNWOO ; Dong han BAE
Korean Journal of Obstetrics and Gynecology 2010;53(1):90-94
Collision tumor means the coexistence of two adjacent, but histologically distinct tumors without histologic admixture in the same tissue and is rare incidence involving ovary. Because of their incidence of occurrence, benign cystic teratomas often occur coincidentally with other abnormalities of the ovary. Most common histologic combination of collision tumor in the ovary is coexistence of teratoma with mucinous tumors. But its association with serous tumor has been noted rare and incidence is unknown. We have experienced a case laparoscopic treatment of a huge serous cystadenoma combined with ovarian benign cystic teratoma in right ovary, and report it with a brief review of literature.
Cystadenoma, Serous
;
Dermoid Cyst
;
Female
;
Incidence
;
Laparoscopy
;
Mucins
;
Ovary
;
Teratoma
9.A case of treatment of peritoneal trophoblastic tissue implants after laparoscopic salpingectomy.
Seong Taek MUN ; Yun Sook KIM ; Seob JEON ; Seung Do CHOI ; Jae Gun SUNWOO ; Dong Han BAE
Korean Journal of Obstetrics and Gynecology 2008;51(4):472-475
The incidence of ectopic pregnancy has increased considerably a decade. And treatment has changed from salpingectomy by laparotomy to conservative laparoscopic surgery or medical treatment. This procedure has many advantages, but there are also disadvantages with the conservative approach and several studies have reported a higher incidence of residual trophoblastic tissue remaining in the tube after treatment. Postoperative follow up of human chorionic gonadotropin (hCG) levels and ultrasonography are needed. This report describes a rare case of persistent trophoblastic implants to the ovary and peritoneum 40 days after laparoscopic salpingectomy. Treatment consisted of laparoscopic excision with biopsy forceps and bipolar, followed by medical treatments. So we report with a brief review of literatures.
Biopsy
;
Chorionic Gonadotropin
;
Female
;
Follow-Up Studies
;
Incidence
;
Laparoscopy
;
Laparotomy
;
Ovary
;
Peritoneum
;
Pregnancy
;
Pregnancy, Ectopic
;
Pregnancy, Tubal
;
Salpingectomy
;
Surgical Instruments
;
Trophoblasts
10.Complete tubular duplication of colon in an adult: a rare cause of colovaginal fistula.
Hae Il JUNG ; Hyoung Uk LEE ; Tae Sung AHN ; Jong Eun LEE ; Hyun Yong LEE ; Seong Taek MUN ; Moo Jun BAEK ; Sang Ho BAE
Annals of Surgical Treatment and Research 2016;91(4):207-211
Alimentary tract duplications are uncommon congenital anomalies that usually present during the first decade of life. Complete duplication of the colon in adults is very rare and difficult to diagnose preoperatively. We report a case of a 40-year-old female with complete tubular duplication which was initially misdiagnosed as a salpingeal abscess due to colovaginal fistula.
Abscess
;
Adult*
;
Colon*
;
Female
;
Fistula*
;
Humans