No abstract available.
Fever* ; Hemoptysis*

Interferon preparations, in addition to their antiviral properties, may inhibit cell growth and multiplication, enhance the expression of cell surface antigens and influence some functions of T-1ymphocytes, macrophages and natural killer cells. The study was performed in order to investigate the cytolytic effect, antiproli ferative effect and HLA antigen expression effect of the gamma-interferon(IFN- r ) on human melanoma cell line A-375. the cytolytic activities were checked by 'Cr release assay, the antiproliferative activities were analyzed by the 'H-thymidine uptake test and HLA antigen expressions were observed by the indirect imrnuno fluor esent method. The results were as follows : 1. The cytolytic effect of the peripheral rnononuclea.r cells treated with INF- on A 375 human melanoma cell line was increased to 31.4%, 36.5%, 33.9%, 53.9 %, 13.9% respectively in the experiment 1, 2, 3, 4, 5 as compared to control group (-11.4%, 3.6%, 18.5%, 35.5%, 6.7% respectively). 2. 1ri the dose response check of the peripheral mononuclear cells treated with INF r to A 375, the cytolytic effect was definitely observed at 200U/ml concentration of the INF r and maximal effect was observed at 500U/ml concentration. 3. The IIUF r treated natural killer cells did not show any significant increase in cytolytie activity as compared to that of untreated natural killer cell and the same results were obtained with monocytes. This cytolytic activity was significantly increased when INF-r treated natural killer cells were co-culturd with monoeytes. 4. The cyaolytjc activity of natural killer cells was increased at the same degree when using culture supernant of INF- treated monocytes as in the case of using
Antigens, Surface ; Cell Line ; Humans* ; Interferons* ; Killer Cells, Natural ; Macrophages ; Melanoma* ; Monocytes

Two typical cases of epithelioid sarcoma were examined by immunohistochemical stain using antibodies to epithelial membrane antigen, carcinoembryonic antigen, vimentin and cytokeratin. Both cases showed positive reactivity for the four kinds of antibodies. These results point to the fact that epithelioid sarcoma simultaneously expresses epithelial markers and characteristic mesenchymal phenotypes. Epithelioid sarcoma appears to be a tumor derived from a multipotential mesenchymal cell with multidirectional differentiation.
Antibodies ; Carcinoembryonic Antigen ; Keratins ; Mucin-1 ; Phenotype ; Sarcoma* ; Vimentin

No abstract available.
Decompression* ; Facial Nerve* ; Melkersson-Rosenthal Syndrome*

No abstract available.
Dental Enamel* ; Lasers, Solid-State*

PURPOSE: The present study investigated whether an autophagic process is involved in the apoptotic death of human tenon's capsule fibroblasts (HTCFs) caused by mitomycin-C. METHODS: An autophagic phenotype was tested using fluorescence microscopy and flow cytometry with specific biological staining dyes including monodansylcadaverine and acridine orange and microtubule-associated protein 1 light chain 3 (LC3). RESULTS: Treatment with mitomycin-C (0.4 mg/ml) increased the acidic vesicular organelles of tenon's capsule fibroblasts in a time dependent manner. Mitomycin-C induced both LC3-II cleavage and beclin-1 expression. 3-MA, a pharmacological inhibitor of autophagy, inhibited the mitomycin-C induced increase of acidic vesicular organelleS. CONCLUSIONS: Autophagy was induced with 0.4 mg/ml mitomycin-C in tenon's capsule fibroblasts. And, autophagic mechanisms may be involved in the early stage of apoptosis of fibroblasts.
Acridine Orange ; Apoptosis ; Autophagy ; Cadaverine ; Coloring Agents ; Fibroblasts ; Flow Cytometry ; Humans ; Light ; Microscopy, Fluorescence ; Microtubule-Associated Proteins ; Mitomycin ; Organelles ; Phenotype ; Tenon Capsule

No abstract available.
Cochlear Implants*

PURPOSE: The purpose of this study was to describe the anglographic finding and clinical result of transarterial embolotherapy(TAE) in patients with injuries of renal artery branches by blunt trauma. MATERIALS AND METHODS: The study was based on retrospective analysis of seven cases, in which TAE was attempted for the control of traumatic renal arterial bleeding. All procedures were performed via the transfemoral approach. TAE was performed with stainless steel coil in two cases, Gelfoam in one case, and Gelfoam and stainless steel coil in four cases. RESULTS: Angiographic findings of vascular injuries were pseudoaneurysm in four cases, extravasation in two cases and arteriocalyceal fistula in one case. All procedures were performed successfully without complication. CONCLUSION: Pseudoaneurysm is a common anglographic finding in patients with injury of renal artery branches and TAE is considered a safe and effective method for treating such cases.
Aneurysm, False ; Embolization, Therapeutic* ; Fistula ; Gelatin Sponge, Absorbable ; Hemorrhage ; Humans ; Renal Artery* ; Retrospective Studies ; Stainless Steel ; Vascular System Injuries

More than 90% of bladder cancers are transitional cell carcinomas. Transitional cell carcinoma of the urinary bladder ordinarily does not pose diagnostic difficulties and is well known its clinical behavior. However, variation from the classic histology can cause problems in recognition. Recently several variants of transitional cell carcinoma of the urinary bladder have been described to promote the recognition of, or to draw attention to, possible differences in clinical behavior. The presence of a micropapillary component in transitional cell carcinoma is associated with high-stage and high-grade transitional cell carcinoma with a tendency of vascular invasion, and with poor prognostic histologic feature. We report a case of micropapillary transitional cell carcinoma of the urinary bladder.
Carcinoma, Transitional Cell* ; Urinary Bladder Neoplasms ; Urinary Bladder*

In male reproducible health, fertility and IVF (in-vitro fertilization), semen analysis has been most important. Semen analysis can be divided into concentration, motional and morphological analysis of sperm. The existing method which was developed earlier to analyze semen concentrated on the sperm motility analysis. To provide more useful and precise solutions for clinical problems such as infertility, semen analysis must include sperm morphological analysis. But the traditional tools for semen analysis are subjective, imprecise, inaccurate, difficult to standardize, and difficult to reproduce. Therefore, with the help of development of microcomputers and image processing techniques, we developed a new sperm morphology analyzer to overcome these problems. In this study the agreement on percent normal morphology was studied between different observers and a computerized sperm morphology analyzer on a slide-by-slide basis using strict criteria. Slides from 30 different patients from the SNUH andrology laboratory were selected randomly. Microscopic fields and sperm cells were chosen randomly and percent normal morphology was recorded. The ability of sperm morphology analyzer to repeat the same reading for normal and abnormal cells was studied. The results showed that there was no significant bias between two experienced observers. The limits of agreement were 4.1%~-3.8%. The Pearson correlation coefficient between readers was 0.79. Between the manual and sperm morphology analyzer, the same findings were reported. In this experiments the slides were stained by two different methods, PAP and Diff-Quik staining methods. The limits of agreement were 7.2%~-5.7% and 6.0%~-6.3%, respectively. The Pearson correlation coefficients ware 0.76 and 0.91, respectively. The limits of agreement was tighter below 20% normal forms. In the experiments of repeatability, 52 cells stained by PAP and Diff-Quik staining methods were analyzed three times in succession. Estimating pairwise agreement, the kappa statistic for the pairs were 0.76, 0.81, 0.86, and 0.75, 0.88, 0.88 respectively. In this study it was shown that there was good agreement between manual and computerized assessment of normal and abnormal cells. The repeatability and agreement per slide of computerized sperm morphology analyzer was excellent. The computer's ability to classify normal morphology per slide is promising. Based on results obtained, this system can be of clinical value both in andrology laboratories and IVF units.
Andrology ; Bias (Epidemiology) ; Fertility ; Humans ; Infertility ; Male ; Microcomputers ; Semen ; Semen Analysis ; Sperm Motility ; Spermatozoa*