In order to evaluate the prevalence of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections in terminally failing hearts, we screened the explanted hearts of transplantation recipients for the presence of HBV DNA and HCV RNA. DNA and RNA extractions were taken from explanted failing hearts (N=7) and normal hearts (N=). Polymerase chain reaction (PCR) and in situ PCR of HBV or reverse transcription-polymerase chain reaction (RT-PCR) and in situ RT-PCR of HCV were performed. The positivity of HBV in failing hearts was 63% (17/27) and it was 50% (3/6) in normal hearts in PCR. There was no significant difference in the positivity of HBV DNA between failing and normal hearts. The positivity of HCV in failing hearts was 18.5% (5/27) and it was 16.7% (1/6) in normal hearts in nested RT-PCR. There was no significant difference in the positivity of HCV RNA between failing and normal hearts. HCV was very rarely observed in explanted terminally failing hearts and HBV was frequently found in both explanted failing hearts and normal hearts. We concluded that these viruses have little direct causal relationship with the development of heart failure.
DNA ; Heart Failure ; Heart* ; Hepacivirus ; Hepatitis B virus ; Hepatitis B* ; Hepatitis* ; Humans* ; Mass Screening* ; Polymerase Chain Reaction ; Prevalence ; RNA

No abstract available.
Constriction, Pathologic* ; Diagnosis*

We report a case of an erythrodermic form of mycosis fungoides in a 68-year-old male, who showed generalized erythroderma with scales for 3 months. Lymphadenopathies in the inguinal and neck areas were present. Histopathological findings showed epidermotropism, perivascular atypical lymphocyte infilteration in the upper dermis, and dermatopathic lymphadenopathy in the inguinal lymph node. T cell markers were positively stained in the immunohistochemical study. These clinicopathological features were consistent with an erythrodermic form of mycosis fungoides(TNM IIl). The patient was treated with PUVA therapy and low dose chemotherapy.
Aged ; Dermatitis, Exfoliative ; Dermis ; Drug Therapy ; Humans ; Lymph Nodes ; Lymphatic Diseases ; Lymphocytes ; Male ; Mycosis Fungoides* ; Neck ; PUVA Therapy ; Weights and Measures

BACKGROUND: In order to evaluate the prevalence of enterovirus and cytomegalovirus infections to terminally failing hearts, the presence of enteroviral RNA and cytomegaloviral DNA was screened in the explanted hearts of transplantation recipients. METHODS: RNA and DNA extractions were performed from explanted failing hearts (N=22) and normal hearts (N=5). Reverse transcription-polymerase chain reaction (RT-PCR) of enterovirus and polymerase chain reaction (PCR) of cytomegalovirus were performed. In situ RT-PCR and in situ PCR were performed with positive nucleic acids of viruses. RESULTS: The positivity of enterovirus in failing hearts was 4.4% (1/22) and 0% (0/5) in normal hearts in nested RT-PCR. There was no significant difference in positivity of enteroviral RNA between failing and normal hearts. Nuclei of myocardium was stained in dark-violet color with in situ RT-PCR. The positivity of cytomegalovirus in failing hearts was 45% (10/22) and 40% (2/5) in nested PCR. There was no significant difference in positivity of cytomegaloviral DNA between failing and normal hearts. Nuclei of myocardium was stained in dark-violet color with in situ PCR. Positive chambers of cytomegalovirus were in decreasing tendency according to increasing patient's age. CONCLUSION: Enterovirus was very rarely observed in explanted terminally failing hearts and cytomegalovirus was frequently found both in explanted failing hearts and normal. These viruses have little direct causal relationship with the development of heart failure.
Cardiomyopathy, Dilated ; Cytomegalovirus Infections* ; Cytomegalovirus* ; DNA ; Enterovirus* ; Heart Failure ; Heart* ; Humans* ; Mass Screening* ; Myocardium ; Nucleic Acids ; Polymerase Chain Reaction ; Prevalence ; RNA

PURPOSE: This study was done to identify the effectiveness of CPR training for nurses in the 6-sigma course. METHOD: The participants were nurses and doctors who worked in the medical center. For data analysis, the SPSS/PC14.0 program was used. RESULT: The total 11 categories of the primary problems were revealed on the recognition of carrying out CPR by nurses and doctors. After understanding the nurses' CPR knowledge and the practical ability, the survey showed 14.97 points and 67.12 points. After the education, the scores were increased from 14.87 to 23.25 in knowledge and from 67.12 to 93.43 in practical ability. The 6-sigma problem was ended as worked up the management plan and understood the changes over the management improving team. CONCLUSION: This study carries an emphasis on understanding of various CPR performance problems applied with 6-sigma in ward. In addition, improvement activities in relation to policy of hospitals suggest the high possibilities that the application of the improvement activities could be extended into the entire hospitals.
Cardiopulmonary Resuscitation ; Lifting ; Statistics as Topic

PURPOSE: This study was done to identify the effectiveness of CPR training for nurses in the 6-sigma course. METHOD: The participants were nurses and doctors who worked in the medical center. For data analysis, the SPSS/PC14.0 program was used. RESULT: The total 11 categories of the primary problems were revealed on the recognition of carrying out CPR by nurses and doctors. After understanding the nurses' CPR knowledge and the practical ability, the survey showed 14.97 points and 67.12 points. After the education, the scores were increased from 14.87 to 23.25 in knowledge and from 67.12 to 93.43 in practical ability. The 6-sigma problem was ended as worked up the management plan and understood the changes over the management improving team. CONCLUSION: This study carries an emphasis on understanding of various CPR performance problems applied with 6-sigma in ward. In addition, improvement activities in relation to policy of hospitals suggest the high possibilities that the application of the improvement activities could be extended into the entire hospitals.
Cardiopulmonary Resuscitation ; Lifting ; Statistics as Topic

No abstract available.
Animals ; Cattle* ; Yersinia enterocolitica* ; Yersinia*