Anthrax toxin consists of three separate proteins, protective antigen (PA), edema factor (EF), and lethal factor (LF). PA binds to the receptor on mammalian cells and facilitates translocation of EF or LF into its cytosol. PA is the primary component of anthrax vaccines. In this study we purified PA from culture filtrates of Bacillus anthracis. The purification involved sequential chromatography through hydroxylapatite, DEAE-Sepharose CL-4B, followed by Mono-Q. The purified PA was judged to be homogeneous on SDS-PAGE, and consisted of a single polypeptide chain with a relative molecular weight of 85,000.
Anthrax ; Anthrax Vaccines ; Bacillus anthracis* ; Bacillus* ; Chromatography ; Cytosol ; Durapatite ; Edema ; Electrophoresis, Polyacrylamide Gel ; Molecular Weight

PURPOSE: We reviewed the effects of resistance training on obesity index, lipid index, blood pressure, pulse rate, and bone mineral densitometer in middle-aged women. METHODS: Randomized, controlled trials lasting 12 weeks investigating the effects of resistance training obesity index, lipid index, blood pressure, and bone densitometer in middle-aged women and published in a peer-reviewed journal up to March 2018 were included. Random- and fixed-effects models were used for analyses, with data reported as weighted means and 95% confidence limits. RESULTS: We included 8 randomized, controlled trials, involving 18 study groups and 335 participants. Overall, resistance training induced a significant low-density lipoprotein reduction [−13.44 (95% CI=−26.71~−0.18)]. No significant effect was observed on other blood lipids, obesity index, blood pressure, pulse rate, and bone mineral density. When study groups were divided according to the period of training, resistance training groups for 24 weeks resulted in a significant decrease in systolic blood pressure [−6.19 (95% CI=−10.95~−1.43 mm Hg)]. CONCLUSION: This meta-analysis supports the potential of resistance training to reduce the level of low-density lipoprotein in middle-aged women. Based on these results, we suggest the application of comprehensive management strategies such as aerobic exercise, resistance exercise, behavior control, and dietary management to prevent obesity in middle-aged women.
Behavior Control ; Blood Pressure ; Bone Density ; Exercise ; Female ; Heart Rate ; Humans ; Lipoproteins ; Miners ; Obesity ; Resistance Training

Purpose: This study aimed to analyze the profiles and mechanisms of injuries using online injury surveillance system (OISS) with match video files at 2017 World Taekowndo Championship (WTC) athletes. Methods: This study design was a prospective epidemiological study with video analysis. All injuries were recorded during the 2017 WTC using the OISS developed by the International Olympic Committee. A total of 971 athletes who participated in the 2017 WTC were enrolled. Each injury was recorded retrospectively by three cameras surrounding each court. Injury profiles and mechanisms were calculated as; percent injury rate, injury rate/100 athlete-exposures, injury location, type, and mechanism via the injury surveillance system and video analysis form. Results: There were 74 injuries, which corresponded to an overall incidence of 13.5 injuries (95% confidence interval, 11.2–15.8) per 100 athletes. The face (27.0%), hand/finger (23.0%), knee (14.9%), and ankle (10.8%) were most frequently injured parts. Contusions (37.8%), fracture and ligament sprains (20.3%) were the most frequently injury types. The main mechanism for contact injury is while attacking with the roundhouse kick (66.2%), or not used block (81.1%). There were also noncontact injuries (24.3%). Conclusion The incidence of injuries to the face, hand/fingers, knee, and ankle was relatively high. To prevent contact injuries, protective headgear and better shin guards and hand protectors need to be developed. As for the noncontact injuries, we recommend hamstring strengthening exercises, and neuromuscular training to strengthen the tendons and ligaments protecting the ankle and knee joints.

No abstract available
Dermatitis, Atopic* ; Eczema* ; Humans ; Nipples*

BACKGROUND: Melanocytes grown in pure monolayer culure lack many of the cellular interactions that exist in vivo. This can be partially overcome by growing melanocytes together with other epidermal cells in skin equivalent models. OBJECTIVE: The objective of the present study was to grow human melanocytes in human epidermis reconstructed on dermal substrates in vitro and to examine their response to UV radiation. METHODS: The skin equivalents were prepared by seeding cultured human keratinocytes together with cultured human melanocytes(in a ratio of 5%) onto de-epidermized dermis. After 7 days of culture, they were exposed to UVB irradiation(total 150m J/cm over 5days). On day 12 of air exposure the sections of the skin equivalents were prepared for histology. The structure of the skin equivalents was studied following staining with hematoxylin and eosin. Melanocytes were characterized by DOPA staining and by immunohistochemistry. RESULTS: Melanocytes were localized singly within the basal layer of the reconstructs. Melanin was also visible both in the melanocytes and in neighboring keratinocytes. There was an increase in melanocyte size and dendricity following UV irradiation. Melanocytes became positive to staining with HMB-45 antibody following UV irradiation. CONCLUSION: Our results indicate that melanocytes grown in reconstructed human epidermis are functional and capable of responding to UV irradiation.
Dermis ; Dihydroxyphenylalanine ; Eosine Yellowish-(YS) ; Epidermis* ; Hematoxylin ; Humans* ; Immunohistochemistry ; Keratinocytes ; Melanins ; Melanocytes* ; Skin