1.Transplantation of Cultured Allogenic Chondrocyte-Collagen Gel Composite into the Articular Cartilage Defect of Rats.
In Ho SEONG ; Sang Cheol SEONG ; Myung Chul LEE ; Hyun Cheol OH ; Kye Yong SONG ; Kui Won CHOI
Journal of Korean Orthopaedic Research Society 1999;2(2):87-101
As one of many cell-many cell-based cartilage repairing methods, transplantation of chondrocyte-embedded-collagen gels in cartilage defect was performed for more satisfactory regeneration of cartilage. The authors performed this study to investigate whether the TGF-beta1 treatment of chondrocytes can do some additional synergistic effect on the transplantation of chondrocyte-embedded-collagen gels for crtilage repair. Chondrocytes were isolated from the articular cartilage of newborn Sprague-Dawley rats. Chondrocytes cultured for 10 days in monolayer were embedded in the 0.45% type I collagen gel. Full-thickness cartilage defect was made in the patellar groove of adult Sprague-Dawley rats. Chondrocytes culdefect was made in the patellar groove of adult Sprague-Dawley rats. The cartilage defects were treated with the following methods in a total of 200 animals, which were assigned to 5 different groups of 40 rats. In the control group, the deffect was left without any treatment, in group I, the defect was filled with collagen gel only, in group II, with collagen gel coontaining 10 ng/ml concentration of TGF-beta1, in group III, with collagen gel containing chondrocytes, and in group IV, with collagen gel containing chondrocytes and TGF-beta1. At 1, 2, 4, 8, 12 weeks after the operation, eight rats of each group were sacrificed, and their distal femurs were harvested for the histologic and biomechanical tests. The section s were stained with hematoxilin and eosin. Alcian-blue, and Safranin-O. Regenerated cartilage was analyzed by the semiquantitative histological grading system. Point indentation test was performed as a biomechanical evaluation, and the stiffness was calculated. The results of the histological grading system revealed that the scores gradually increased with time in all groups, and the scores of group III and IV were higher than those of control, group I and II. The biomechanical study showed that the stiffness gradually increased to reach a plateau level in each group. In control, group I and II, the stiffness increased up to the eighth week and remained around the increased level at the twelfth week, and did not show any statistically significant difference between the groups. In group III and IV, the stiffness was higher than in control group, and increased markedly at the fourth week and the increased level was maintained onwards. The results of this study showed that the transplantation of chondrocyte-embedded-collagen gels enhanced the healing process, and the treatment of TGF-beta1 demonstrated at least partially significant improvement.
Adult
;
Animals
;
Cartilage
;
Cartilage, Articular*
;
Chondrocytes
;
Collagen
;
Collagen Type I
;
Eosine Yellowish-(YS)
;
Femur
;
Gels
;
Humans
;
Infant, Newborn
;
Rats*
;
Rats, Sprague-Dawley
;
Regeneration
;
Transforming Growth Factor beta1
2.Modification of Immune Response by Cold Stress in Mice.
Young Min PARK ; Yang Hyo OH ; Young Ran SEONG ; Jung Cheol LEE ; Seol Hyang BAEK
Korean Journal of Immunology 1997;19(4):437-446
No abstract available.
Animals
;
Interleukin-2
;
Mice*
3.Objective measurenment with stabilometry of dizzy symptoms(1): Based on stabilometry.
Sang Cheol LEE ; Bong Whan OH ; Seong Soo KIM ; Sang Mok LEE
Korean Journal of Otolaryngology - Head and Neck Surgery 1993;36(4):612-623
No abstract available.
4.Clinical Evaluation of Surgical Treatment for Hemoptysis Patients.
Sung Joo LEE ; Seong Cheol MOON ; Dae Sig KIM ; Seong Cheol OH ; Chang Hoe KIM ; Sung Soo CHAE
The Korean Journal of Thoracic and Cardiovascular Surgery 1997;30(11):1097-1104
The expectoration of blood is always a fearful experience for the patient and a matter of grave concern to the attending physician, because it may be the warning sign of serious diseases. When such bleeding occurs, the possibility of its being due to pulmonary tuberculosis, bronchiectasis or carcinoma of the lung is promptly suggested. Nowadays, with the advance of modern diagnostic studies, differential diagnosis has become possible for the hemoptysis patients to have appropriate treatment. This evaluation is based on the review of 75 cases of patients whom we performed emergency open thoracotomies for 6 years from March 1992 to February 1997 in the Department of Thoracic and Cardiovascular Surgery, Seoul Adventist Hospital. The mean age of the patients was 36.6 years old with a range from 19 to 68, and most prevalent age group was thirties. The most common underlying lung disease causing hemoptysis was tuberculosis(44%). Most accurate diagnostic study was bronchoscopy during hemoptysis(95%) and right middle and lower lobe(17.3%) was the most common site of lesion. Lobectomy(50%) was the most frequent operative method and recurrent hemoptysis(31.6%) was the most common postoperative complication. But most of the patients(82.6%) were completely recovered by surgical treatment. Now we concluded that the proper management of hemoptysis was completed by surgical approach with definite diagnosis and supportive medication.
Bronchiectasis
;
Bronchoscopy
;
Diagnosis
;
Diagnosis, Differential
;
Emergencies
;
Hemoptysis*
;
Hemorrhage
;
Humans
;
Lung
;
Lung Diseases
;
Postoperative Complications
;
Seoul
;
Thoracotomy
;
Tuberculosis, Pulmonary
5.A Study on Usage of Health Improving Agents in Seoul & Busan.
Seong Cheol PARK ; Mi Young OH ; Hak Soo KIM
Journal of the Korean Dietetic Association 2005;11(4):440-448
This study explores some basic issues behind adults' seeking and using patterns of alternative medicine as well as health food (health food/medicine). In order to do this, 791 adult participants in Seoul and Busan were interviewed face-to-face. The results of the survey showed that 1) interpersonal influence was the most influential factor in relation to the adoption of health food/medicine(46.9% of the participants reported on the influences), 2) keeping healthy was the main motivation for the usage of health food/medicine(34.5% of the participants), 3) mass media was the important information source for health food/medicine, 4) with regard to trustworthiness of information sources, experts were believed to be the most trustworthy while information from acquaintances were thought less, and finally, 5) pharmacies and health food stores were main suppliers of health food/medicine. This study suggests some marketing strategies for health food/medicine. For example, it can be suggested that interpersonal communication among other information channels should be focused and might be increased trust by using professionals.
Adult
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Busan*
;
Complementary Therapies
;
Dietary Supplements
;
Friends
;
Food, Organic
;
Humans
;
Marketing
;
Mass Media
;
Motivation
;
Pharmacies
;
Seoul*
6.The Effect of Estrogen on Antioxidant Enzymes in Bone of Oophorectomized Rats.
Young Do KOH ; Se In OH ; Mee Sook LEE ; Chang Mo KANG ; Chung Shil KWAK ; Sang Cheol SEONG ; Sang Chul PARK
The Journal of the Korean Orthopaedic Association 1998;33(1):148-157
The purpose of this study is to evaluate an antioxidative effect of estrogen on the bone in oophorectomized rats. Thirty Sprague-Daley rats were equally divided into 3 groups; group 1 as control group with sham operation, group 2 as experimental group with oophorectomy, and group 3 as oophorectomized group treated with estrogen. Estradiol (5mg/kg BW) was administered three times per week from first to sixth week after oophorectomy. Left tibia was obtained to measure the amount of protein carbonyls as an index of oxidative stress and the activity of antioxidant enzymes. The results were as follows: trahecular bone area in proximal tihia decreased after oophorectomy, which increased in response to estrogen administration. The level of protein carbonylation in hone was not significantly different among all groups. Activity of antioxidant enzymes such ais glutathione reductase(GR), glutathione peroxidase(GP) and glutathione transferase(GST) in bone was not significantly different among all groups. However, the activity of catalase in bone markedly increased in group 3 compared with that in group 1 and group 2. In summary, bone trabecular area increased after admin- istration of estrogen. And estrogen induced the activitv of catalase, which might contrihute to prevent the oxidative damage. However, the glutathione utilizing enzymes such as GR, GP and GST were not significantly affected by estrogen status.
Animals
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Catalase
;
Estradiol
;
Estrogens*
;
Female
;
Glutathione
;
Ovariectomy
;
Oxidative Stress
;
Protein Carbonylation
;
Rats*
;
Tibia
7.The reliability of health risk appraisals questionnaire: in SNUH lifetime health-monitoring program.
Seong Ho HA ; Jae Hyun PARK ; Hyun Joo OH ; Cheol Hwan KIM ; Sang Im JEON ; Lack Jin SEONG ; Taiwoo YOO ; Chang Yup KIM
Journal of the Korean Academy of Family Medicine 1992;13(4):354-363
No abstract available.
Health Status Indicators*
;
Surveys and Questionnaires
8.Treatment of Diffuse In-stent Restenosis with Rotational Atherectomy Followed by Radiation Therapy with a 188Re-MAG3-Filled Balloon.
Seong Wook PARK ; June Hong KIM ; Siwan CHOI ; Myeong Ki HONG ; Dae Hyuk MOON ; Seung Jun OH ; Cheol Whan LEE ; Jae Joong KIM ; Seung Jung PARK
Korean Circulation Journal 2001;31(5):466-475
BACKGROUND: Rotational atherectomy has been shown to be safe and efficient for the treatment of ISR, but the recurrence rate is still high. Intracoronary-irradiation after rotational atherectomy may be a reasonable approach to prevent recurrent ISR. SUBJECTS & METHODS: Fifty consecutive patients with diffuse ISR (length > 10 mm) in native coronary arteries underwent rotational atherectomy and adjunctive balloon angioplasty followed by beta irradiation using a 188Re-MAG3-filled balloon catheter. The radiation dose was 15 Gy at 1.0 mm deep into vessel wall. RESULTS: Mean length of the lesion and irradiated segment was 25.6 +/- 12.7 mm and 37.6 +/- 11.2 mm, respectively. The radiation was delivered successfully to all patients, with a mean irradiation time of 201.8 61.7 seconds. No adverse event including myocardial infarction, death, or stent thrombosis occurred during the follow-up period (mean 10.3 +/- 3.7 months) and non-target vessel revascularization was needed in one patient. Six-month binary angiographic restenosis rate was 10.4 % and loss index was 0.17 +/- 0.31. CONCLUSIONS: beta irradiation using 188Re-MAG3-filled balloon following rotational atherectomy is safe and feasible for patients with diffuse ISR, and it may improve the clinical and angiographic outcomes. Further prospective randomized trials are warranted to evaluate the synergistic effect of debulking and irradiation in patients with diffuse ISR.
Angioplasty, Balloon
;
Atherectomy, Coronary*
;
Catheters
;
Coronary Vessels
;
Follow-Up Studies
;
Humans
;
Myocardial Infarction
;
Recurrence
;
Stents
;
Thrombosis
9.Simplified Method to Measure the Peripapillary Choroidal Thickness Using Three-dimensional Optical Coherence Tomography.
Jaeryung OH ; Chungkwon YOO ; Cheol Min YUN ; Kyung Sook YANG ; Seong Woo KIM ; Kuhl HUH
Korean Journal of Ophthalmology 2013;27(3):172-177
PURPOSE: To evaluate a simplified method to measure peripapillary choroidal thickness using commercially available, three-dimensional optical coherence tomography (3D-OCT). METHODS: 3D-OCT images of normal eyes were consecutively obtained from the 3D-OCT database of Korea University Medical Center On the peripapillary images for retinal nerve fiber layer (RNFL) analysis, choroidal thickness was measured by adjusting the segmentation line for the retinal pigment epithelium to the chorioscleral junction using the modification tool built into the 3D-OCT image viewer program. Variations of choroidal thickness at 12 sectors of the peripapillary area were evaluated. RESULTS: We were able to measure the peripapillary choroidal thickness in 40 eyes of our 40 participants, who had a mean age of 41.2 years (range, 15 to 84 years). Choroidal thickness measurements had strong inter-observer correlation at each sector (r = 0.901 to 0.991, p < 0.001). The mean choroidal thickness was 191 +/- 62 microm. Choroidal thickness was greatest at the temporal quadrant (mean +/- SD, 210 +/- 78 microm), followed by the superior (202 +/- 66 microm), nasal (187 +/- 64 microm), and inferior quadrants (152 +/- 59 microm). CONCLUSIONS: The measurement of choroidal thickness on peripapillary circle scan images for RNFL analysis using the 3D-OCT viewing program was highly reliable and efficient.
Adolescent
;
Adult
;
Aged
;
Aged, 80 and over
;
Choroid/*anatomy & histology
;
Databases, Factual
;
Female
;
Humans
;
Imaging, Three-Dimensional/*methods/statistics & numerical data
;
Male
;
Middle Aged
;
Observer Variation
;
Retina/*anatomy & histology
;
Tomography, Optical Coherence/*methods/statistics & numerical data
;
Young Adult
10.The Effects of Alcohol on Visual Evoked Potential and Multifocal Electroretinography.
Jee Taek KIM ; Cheol Min YUN ; Seong Woo KIM ; Jaeryung OH ; Kuhl HUH
Journal of Korean Medical Science 2016;31(5):783-789
The aim of this study was to investigate the acute effects of ethanol administration on pattern-reversal visual evoked potential (VEP) and multifocal electroretinography (mfERG). Fifteen healthy subjects with no ocular or general disease were recruited. VEP (0.25° pattern sizes) and mfERG with 19 elements in two recording segments were performed before ethanol administration to obtain baseline for each participant. A few days later, the participants visited again for VEP and mfERG measurements after ethanol administration. Ethanol (0.75 g/kg) was administered orally over the course of 30 minutes. VEP and blood alcohol concentration were evaluated one hour after ethanol administration, and mfERG was conducted after pupil dilation. The Wilcoxon signed-rank test was used to compare parameter changes after randomized eye selection. The mean blood alcohol concentration was 0.034% ± 0.05% by volume. VEP revealed a P100 latency delay (109.4 ± 5.3; 113.1 ± 8.2; P = 0.008) after alcohol administration. The P1 implicit time of ring 1 on mfERG showed a trend of shortening after alcohol administration (37.9 ± 1.0; 37.2 ± 1.5; P = 0.048). However, the changes did not show statistical significance after Bonferroni correction. In conclusion, orally administrated ethanol (0.75 g/kg) appears to suppress the central nervous system, but it is not clear whether alcohol intake affects the retina.
Adult
;
*Alcohol Drinking
;
Electroretinography
;
Evoked Potentials, Visual/*physiology
;
Female
;
Humans
;
Male
;
Retina/physiology