No abstract available.

Serum samples from 123 males and 123 females collected by age in 1996 were analyzed for antibodies against surface antigen of Hepatitis B virus and C22-3, C200 antigens of Hepatitis C virus. Sera from the children under the age of 10 showed 30% seropositivity to the surface antigen of Hepatitis B virus, 33.3% in 10~19 year group, 20% in 20~29 year group, 17.6% in 30~39 year group, 3.3% in 40~49 year group, 5.9% in 50~59 year group, 8,3% in 60~69 year group, 2.9% in 70~79 year group, but antibody could not found in 80~86 year group. 12 out of 123 male sera were positive, 19 out of 123 female sera were positive and overall rate of positivity of antibody against surface antigen of Hepatitis B virus was 12.6%. Serum samples from peoples under the age of 30 had not antibody against C22-3, C200 antigens of Hepatitis C virus. The positivity rate was 2.9% in 30~39 year group. 5 out of 30 sera from 40~49 year age group were positive, and 3 positive sera showed extremely high titer (1:524,288) but the titers of two remaining sera were 1:32, 1:8,192 respectively. 5.9% was positive in 50~59 year group, 8.3% in 60~69 year group, 11.8% in 70~79 year group but all negative in 80~86 yea. group. 6 out of 123 male sera were positive (4.9%), 9 out of 123 female sera were positive (7.3%). Overall .ate of positivity of antibody against C22-3, C200 antigen of Hepatitis C virus was 6.1%. None out of 246 sera had both antibodies against Hepatitis B virus and Hepatitis C virus.
Antibodies* ; Antigens, Surface ; Child ; Female ; Hepacivirus* ; Hepatitis B virus* ; Hepatitis B* ; Hepatitis C* ; Hepatitis* ; Humans* ; Male

No abstract available.
Bronchiolitis* ; Humans ; Immunoglobulin E*

The truncated E1192-283 and E2384-649 genes of hepatitis C virus (HCV) linked to the gene for glutathione 5-transferase (GST) were constructed and their expressions were analyzed. The GST-E1192-283 fusion gene overexpressed the fusion protein in E. coli as a soluble form, while the GST-E1192-383 plasmid did not express expected fusion protein. The purified GST-E1192-283 fusion protein was efficiently cleaved by thrombin. More than 90% pure, HCV E1192-283 protein was obtained by GST-agarose chromatography. The truncated GST-E2384-649 fusion gene expressed the fusion protein mainly as an insoluble form, whereas the GST-E2384-740 did not express the fusion protein. The truncated GST-E1 182-283 and GST-E2384-649 fusion proteins reacted specifically with an HCV patient serum. In addition, mice immunized with either the purified E1192-283 or GST-E2384-649 proteins generated specific antibodies to each antigen. The results suggested that hydrophobic carboxyl portions of the E1 and E2 proteins might affect expression levels as well as the solubility of each fusion protein in bacteria. Also, the truncated E1 protein with Tyr-192 to Ser-283 contained antigenic epitope(s) which could be specifically recognized by an HCV patient serum.
Animals ; Antibodies ; Bacteria ; Chromatography ; Glutathione ; Hepacivirus* ; Hepatitis C* ; Hepatitis* ; Humans ; Mice ; Plasmids ; Solubility ; Thrombin

No abstract available.

Clinically, the Rh blood group system is important since Rh antibodies are readily induced by transfusion or pregnancy in individuals negative for the antigert and may cause hemolytic reactions or hemolytic disease of the newborn. Since the D antigert is strongly immunogenic, donors and patients are routinely typed for D status and patients are generally given D compatible blood. But under several circumstances such as spontaneous agglutination of red blood cells coated with immunoglobulin, antisera with additives may cause false positive results in test using high-protein reagents. And facton in the patient' s own serum may also affect the test, since unwashed red blood cells suspended in their own serum or plasma are frequently tested. Therefore, manufacturers and American Association of Blood Banks(AABB) recommend that the Rh(D) control test with Rh(D) control reagent which contains the same additive present in high-protein anti-D except for the anti-D. This study was undertaken to evaluate the usefss of the Rh(D) control test in Korea where Rh(D) negative population is small. Red blood cells from 1115 in-patients and 468 out-patients at Korea University Medical Center were employed in Rh(D) typing and Rh(D) control test in parellel. 1580 cases are Rh(D) positive and 3 cases were Rh(D) negative. No agglutination was observed with Rh(D) control test. Though AABB and manufacturers recommended that the Rh(D) control test should be done in parellel with Rh(D) typing test, the authers concluded that there were no need to run the Rh(D) control test in Korea.
Academic Medical Centers ; Agglutination ; Antibodies ; Erythrocytes ; Humans ; Immune Sera ; Immunoglobulins ; Indicators and Reagents ; Infant, Newborn ; Korea ; Outpatients ; Plasma ; Pregnancy ; Tissue Donors

No abstract available.
Child* ; Humans

Cholelithiasis and cholecystitis frequently necessitate cholecystectomy. Experience is increasing with laparoscopic cholecystectomy, a technique that avoids much of the morbidity, pain, prolonged hospital day, and recovery complications associated with the traditional approach. And the safety of laparoscopic cholecystectomy was confirmed. Besides appendectomy, cholecystectomy is the second most common nonobstetric procedure performed in pregnant women. We have performed one case of a successful laparoscopic cholecystectomy in the second trimester of pregnancy. Pregnancy was once a contraindication of laparoscopic operation, but no longer.
Appendectomy ; Cholecystectomy ; Cholecystectomy, Laparoscopic* ; Cholecystitis ; Cholelithiasis ; Female ; Humans ; Pregnancy ; Pregnancy Trimester, Second* ; Pregnancy* ; Pregnant Women

Biepicondylar fracture of the humerus is very rare in adults. To date, there have been limited evidence of this injury in the English literature. We report a case of a 65-year-old female with a biepicondylar fracture of the left distal humerus without dislocation. Open reduction and internal fixation with K-wires, cannulated screw, and suture anchor were performed. We obtained stability of the elbow and a satisfactory functional outcome. Because this type of injury is associated with varus and valgus instability, operative reduction and fixation are essential in order to gain stability and early recovery of normal function.
Adult* ; Aged ; Dislocations ; Elbow ; Female* ; Humans ; Humeral Fractures ; Humerus* ; Suture Anchors

A few cases of a dermatosis characterized by "music box spine" keratotic papules limited to the palms and soles have been reported. There have been several names for this dermatosis The Most commonly incorporated term is porokeratosis, but Osman et at suggested the name "spiny keratoderma of palms and soles" because the clinical, histologic and electron microscopic features of this lesion are distinct from porokeratosis. We report a case of spiny keratoderma on the digit showing a single lesion, as a variant of the classic spiny keratoderma of palms and soles.
Porokeratosis ; Skin Diseases