The lack of sufficient oral mucosa available for intra-oral reconstruction has been dealt with by the use of skin or oral mucosa grafts harvested from donor sites but grafts requires more than one surgical procedures and could cause donor site morbidity. Many investigators have attempted to increase available soft tissue by tissue engineered skin or oral mucosa replacements for clinical applications. But, reconstructed mucosa by several methods have low physical properties such as rolling and contraction. The aims of this study were to develope an in vitro experimental model that maintains an epithelial-mesenchymal interaction by organotypic raft culture, and to characterize biologic properties of three-dimensionally cultured oral mucosa embedded with Polydioxanone mesh by histological and immunohistochemical analysis. The results were as follows; 1. Oral mucosa reconstructed by three-dimensional organotypic culture revealed similar morphologic characteristics to equvalent normal oral mucosa in the point that they show stratification and differentiation. 2. The expression of cytokeratin 10/13 and involucrin in the cultured tissue showed the same pattern with normal oral mucosa suggesting that organotypic co-culture condition is able to induce cellular differentiation. 3. After insertion of polydioxanone mesh, increased tensile strength were observed. These results suggest that three-dimensional organotypic co-culture of the oral mucosa cell lines with the dermal equvalent consisting type I collagen and fibroblasts reproduce the morphologic and immunohistochemical characteristics similar to those in vivo condition. And increased physical properties by use of polydioxanone mesh will helpful for clinical applications.
Cell Line ; Coculture Techniques ; Collagen Type I ; Fibroblasts ; Humans ; Keratins ; Models, Theoretical ; Mouth Mucosa* ; Mucous Membrane ; Polydioxanone* ; Research Personnel ; Skin ; Tensile Strength ; Tissue Donors ; Transplants

BACKGROUND: Many physiologic, pharmacologic and immunologic abnormalities were reported in atopic dermatitis but the cause and pathogenesis of the disease remain obscure. OBJECTIVE: This study was done to investigate the systemic immunologic abnormalities in atopic dermatitis. METHOD: To evaluate the cell mediated immunity, me quantified pei ipheral blood T lymphocytes and their subsets, using flow cytometery, and assessed plasma neopteiin levels by means of radioimmunoassay. To evaluate the abnormal humoral immunity, we assessed the serum IgE levels by means of enzyme-immunoassay. RESULTS: Mean proportions of peripheral blood T lymphocytes and, heir subsets in atopic Dermatitis patients were within normal limits. Hut the suppvessor/cytotoxic T lyrphocytes(T8) were significantly decreased in the group of se"ere atopic dermatitis compared with the group of mild atopic dermatitis(P<0.05). Plasma neopterin lervels in the group of atopic dermatitis were found to be significantly elevated as compared vith the control group(P<0.01), but no significant cifference was found between the mild and severe group of atcpic dermatitis(P>0.05). Mean serum IgE levels in the patients with atopic dermatitis were higher than reference value. But there was no significant difference between the mild and severe atopic dermatitis group. Serum IgE levels ivere negatiiely correlated with T8(r=-0.3774, P<0.05) and positively with T4/T8 ratio(r =0.5007, P<0.05). Conclusions : These data;uggest that the atopic der matitis has abr ormalities in cell mediated immunity as well as elevated IgE level.
Dermatitis, Atopic* ; Humans ; Immunity, Cellular ; Immunity, Humoral ; Immunoglobulin E ; Neopterin* ; Plasma* ; Radioimmunoassay ; Reference Values ; T-Lymphocyte Subsets* ; T-Lymphocytes

No abstract available.
Humans ; Inpatients* ; Pediatrics ; Retrospective Studies*

In the unconscious patient with general muscle rigidity, the natural teeth can be luxated and then bite wounds may be occurred around lip, owing to the pathologic involuntary self-biting habit. If the forceful biting is generated continuously, the teeth may be avulsed and aspirated into the aerodigestive tract with the infection of biting wound. For the prevention of pulmonary aspiration of the teeth and wound infection, the biting teeth should be cared before the fact. The authors treated the teeth as endodontic drainage with removal of the crown or iodoform gauze drainage into the socket with extraction of the teeth. The prognosis was more favorable without biting wounds.
Crowns ; Dental Pulp Cavity* ; Drainage* ; Humans ; Lip ; Muscle Rigidity ; Prognosis ; Tooth ; Wound Infection ; Wounds and Injuries*

The experienced surgeon can be surprised & challenged by the hazards of active bleeding during oral & maxillofacial surgical procedure, because of alterations in the surgical anatomy, bleeding disorders and surgical intervention of infected tissues. This is a report of two cases of active bleeding during surgical extraction of mandibular third molar, that had the pericoronitis, osteitis and adjacent neurovascular bundle in its apex. When the abrupt active bleeding was occurred during surgical extraction of mandibular third molar, pressure packing by hemostatic agent(bone wax) & wet gauze biting were applied into the extraction socket during 30 minutes. After 30 minutes, the wound was explored about the bleeding and active bleeding was then continued. In spite of repeated bleeding control method of the pressure dressing, the marked hemorrhage was generated continuously. Therefore, the author decised the bleeding as immediately uncontrollable hemorrhage and the pressure dressing was again applied for the more longer duration without wound closure. After 3 days, the pressure dressing was removed and iodoform gauze drainge was then established without the bleeding. The drain was changed as the interval of 3~5 days for prevention of infection & secondary hemorrhage and relatively good wound healing was then resulted in 6 weeks.
Bandages ; Hemorrhage* ; Molar, Third* ; Osteitis ; Pericoronitis ; Wound Healing ; Wounds and Injuries

No abstract available.
Emergencies ; Humans

PURPOSE: To determine the effectiveness of standard diet therapy as a preventive measure against urinary stone recurrence, we compared the metabolic parameters before and after diet recommendation in the patients with urolithiasis. MATERIALS AND METHODS: A total of 40 stone formers participated in this study and none of them had other metabolic disorders. The dietary recommendations for them included the following: large amounts of water and fruit juice, and restriction of animal protein, salt and oxalate-rich foods. Simplified metabolic evaluations were performed before and after the dietary therapy and compared using the Wilcoxon signed rank test and McNemar's test. The urinary volume and creatinine differences between the two tests were also considered for determining the patients' compliance and appropriate 24-hour urine collection. RESULTS: There was no significant change in the patients blood chemistry before and after the diet therapy. Among the urinary parameters, the excretion of citrate (p=0.005) was significantly increased, but the rest of the parameters remained unchanged. With respect to occurrence of urinary metabolic abnormalities, only the frequency of hypocitraturia showed a statistically significant decrease after diet therapy (p=0.039). The results concerning the changes in the urinary volume and creatinine level showed no difference before and after the therapy. CONCLUSIONS: The standard diet therapy applied to the patients with urolithiasis showed a favorable effect on the excretion of urinary citrate, which is an important protective factor against stone formation. However, we could not found any significant changes in the other urinary lithogenic parameters during this short-term evaluation.
Animals ; Chemistry ; Citric Acid ; Compliance ; Creatinine ; Diet Therapy* ; Diet* ; Fruit ; Humans ; Recurrence ; Risk Factors ; Urinary Calculi ; Urine Specimen Collection ; Urolithiasis* ; Water

No abstract available.
Population Characteristics* ; Thyroid Gland* ; Thyroiditis*

Although Soft tissue contrast and direct multiplanar imaging capability of MRI are well recognized, myelo Been the imaging modality of choice in evaluationg cervical root injury. We assessed the role of M compared its diagnostic accuracy with myelography in the evaluation of cervical root injury. MR finding Root injury in ten patients (55 roots) were retrospectively reviewed. In 26 explored roots (6 patin Frndings were compared with myelography and surgical results. In 29 roots (8 patients), which were By myelography or exploration, the MR findings were focal extraudral CSF collections (pseudomem In 21/29(72.4%, 8 patients), thickening of extradural roots in 4/29 (13.6%, 5 patients), and thickern Darn in 12/29 (41.4%, 6 patients) roots. T2-weighted axial image was superior to Tl weighted and Density-weighted images for delineationg root avulsion. The sensitivity and specificity of MRI were 7 93.3% respectively, while those of myelography were 83% and 90%. Overall diagnostic accuracy of Myelography were comparable (84.6% vs 87.5%). In conclusion, myelography is still considered as the Of choice in the preoperative evaluation of the cervical root avulsion because of its higher sensi, however, may obviate the myelography with some technical refinements.
Humans ; Magnetic Resonance Imaging* ; Myelography ; Retrospective Studies ; Sensitivity and Specificity

Although Soft tissue contrast and direct multiplanar imaging capability of MRI are well recognized, myelo Been the imaging modality of choice in evaluationg cervical root injury. We assessed the role of M compared its diagnostic accuracy with myelography in the evaluation of cervical root injury. MR finding Root injury in ten patients (55 roots) were retrospectively reviewed. In 26 explored roots (6 patin Frndings were compared with myelography and surgical results. In 29 roots (8 patients), which were By myelography or exploration, the MR findings were focal extraudral CSF collections (pseudomem In 21/29(72.4%, 8 patients), thickening of extradural roots in 4/29 (13.6%, 5 patients), and thickern Darn in 12/29 (41.4%, 6 patients) roots. T2-weighted axial image was superior to Tl weighted and Density-weighted images for delineationg root avulsion. The sensitivity and specificity of MRI were 7 93.3% respectively, while those of myelography were 83% and 90%. Overall diagnostic accuracy of Myelography were comparable (84.6% vs 87.5%). In conclusion, myelography is still considered as the Of choice in the preoperative evaluation of the cervical root avulsion because of its higher sensi, however, may obviate the myelography with some technical refinements.
Humans ; Magnetic Resonance Imaging* ; Myelography ; Retrospective Studies ; Sensitivity and Specificity