To delineate the individual role of follicular dendritic cells (FDC) and T cells at each stage of GC B cell differentiation at the clonal level and to analyze the signals required for the differentiation, we developed an experimental model using an FDC line, HK and a lymphoma cell line, L3055 for centroblasts. Phenotypic analysis of L 3055 revealed its origin of GC and the homogeneity. L3055 cells undergo spontaneous apoptosis when cultured in the absence of HK cells. L3055 cells proliferate continuously in the presence of HK cells, while they differentiate into a population with the phenotype of centrocytes after stimulation with CD40 ligand (CD 40L) plus IL-2, IL-4 and IL-10. L3055 undergo anti-Ig-mediated apoptosis, which is not protected by HK but by CD40L and the cytokines. These experimental results suggest that FDC provide signals for the survival and rapid proliferation of centroblasts and T cells trigger the differentiation of centroblasts into centrocytes.
Apoptosis ; CD40 Ligand ; Cell Differentiation* ; Cell Line ; Cytokines ; Dendritic Cells, Follicular ; Germinal Center* ; Interleukin-10 ; Interleukin-2 ; Interleukin-4 ; Lymphoma ; Models, Theoretical ; Phenotype ; T-Lymphocytes

No abstract available.
Urinary Bladder*

No abstract available.
Classification* ; Colorectal Neoplasms* ; Lymph Nodes* ; Neoplasm Metastasis* ; Risk Factors*

No abstract available.
Breast Neoplasms* ; Breast*

No abstract available.

Primary cutaneous cryptococcosis of ulcers and plaques developed on the face of an otherwise healthy 7-year-old boy. Lesions were successfully treated with 10% 5-fluorocytosine ointment under occlusive dressing, Cultures for the organism from the involved area became negative after 10 weeks of treatment but the organism was observed under microscope until 10 weeks after treatment. The extensively ulcerated granulomatous lesions healed with scar formation in 15 weeks. The patient had completed 20 weeks of treatment. Follow-up examination for 1 year showed no evidence of recurrence.
Child ; Cicatrix ; Cryptococcosis* ; Flucytosine* ; Follow-Up Studies ; Humans ; Male ; Occlusive Dressings ; Recurrence ; Ulcer

No abstract available.

As the incidence of dermographism in our urticaria clinie is quite high comparing data in other country, we have tried to survey the incidence of ermographism in the general population of Korea using a dermographic tester designed by other author. Total 8g7 healthy persons were included in this study from March J98$ to .December 1983. The study result was summarized as follows, 1. Male to female ratio was 2. 5: 1 and the peak age of the subjects was third(47 8%), fourth and fith decades in ecreasing order, The overall incidence of dermographisrn from the pressure of 48ppg/cm was 4.4% and there were no significa.nt difference in the incidences between male(4, 2%) and female(4 7%) (p>0. 1), and between the different age groups(p>0 l)
Female ; Humans ; Incidence* ; Korea ; Male ; Urticaria

BACKGROUND: Many of the major life events that appear to affect biologic system and health occur within the context of the family and can have a profound psychosocial and biologic impact on family memebers. So, the present study attempted to examine the relations of family function with stressful life events, depression, and physical and psychological symptoms reported by patients. METHODS: Design : Cross sectional, observational study. Participants : 41 healthy adult males from a family medicine ambulatory care unit in a tertiary hospital located in Guro completed a series of psychometric instruments that include the Family APGAR(Adaptation, Partnership, Growth, Affection, and Resolve), FACESIII(Family Adaptability and Cohesion Evaluation Scale), Modified 46-item Stressful Life Events Scale, Lee's 98 items Stressful Life Event Scale, Korean version of BEPSI(Brief Encounter Psychosocial Instrument) and BDI(Beck's Depression Inventory Scale). RESULTS: Family APGAR was negatively correlated with stress related life events(both in total and negative events, -0.30, -0.37, respectively; p<0.05) and BEPSI(-0.56; p<0.0001). No significant correlation could be found between family function and positive life events. Family APGAR also revealed significant negative correlation with BDI(-0.61; p<0.0001). Among the three family types, severe dysfunctional family demonstrated highest level of stressful life events score, perceived stress, and depression(p<0.05). CONCLUSIONS: The higher the assessment of the family function(Family APGAR), the lower the level of stressful life events, perceived stress and depression. There, knowledge of the family function, stress, and coping could be useful to family physicians in the whole person approach of their patients.
Adult* ; Ambulatory Care ; Depression* ; Humans ; Male* ; Observational Study ; Physicians, Family ; Psychometrics ; Tertiary Care Centers

No abstract available.