1.Study on antigenic analysis and serial antibody titration by using leptospira interrogans isolated at Chungchongbukdo.
Kyung Hee PARK ; Seok Cheol HONG ; Woo Hyun CHANG
Journal of the Korean Society for Microbiology 1991;26(4):317-327
No abstract available.
Chungcheongbuk-do*
;
Leptospira interrogans*
;
Leptospira*
2.A study on the applicability of polymer(polyglactin)for the artificial dermis.
Sung Pyo HONG ; Jae Kyung PARK ; Seung Ho HUH ; Hong Seok KIM
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1998;25(4):547-556
The result of artificial skins made with collagen is poor after grafting over the full thickness wounds due to their rapid degradation by enzymatic cleavage. This study is an in vivo study of an artificial skin made with a biodegradable polymer, which can better address the problem of the collagenous artificial dermis. To investigate the availability of a biodegradable polymer for an artificial dermis and to get an information about the optimal degradation rate of a polymer for an artificial dermis, we made an artificial dermis by seeding of fibroblasts within the vicryl mesh and made a bilayer artificial skin by covering the artificial dermis with cultured keratinocytes. And these artificial dermis and artificial skin were evaluated in a full thickness wound model. The results are as followings: 1. The artificial dermis was available for grafting for 1 week culture of vicryl mesh-fibroblast. 2. The artificial dermis retarded the contraction of full thickness wounds. 3. The artificial dermis generated the granulation tissue and accepted the STSG completely. 4. The generated tissue from the artificial dermis had incorporated into the surrounding tissue by 4 weeks postgrafting. 5. Vicryl in the artificial dermis became to biodegrade from the culture period and absorbed completely by 5 weeks. 6. The epidermal portion was poorly differntiated during in vitro culture period. In conclusion, the polymer-fibroblast graft can retard the wound contraction and generate a new tissue permitting a useful dermal replacement. And to get more optimal results, another polymer which has slower biodegradation rate than vicryl should be used for the artificial dermis and the epidermal portion should be differentiated after in vivo grafting.
Collagen
;
Dermis*
;
Fibroblasts
;
Granulation Tissue
;
Keratinocytes
;
Polyglactin 910
;
Polymers
;
Skin, Artificial
;
Transplants
;
Wounds and Injuries
3.A STUDY ON THE DEVELOPMENT OF BIOSYNTHETIC SKIN REPLACEMENTS CONSISTED OF FIBROBLASTS GROWN IN BIOBRANE.
Hong Seok KIM ; Sung Pyo HONG ; Jae Kyung PARK ; Doo Hyung LEE
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1997;24(5):875-883
No abstract available.
Fibroblasts*
;
Skin*
4.Clinical analysis of contact Nd:YAG laser tonsillectomy.
Hang PARK ; Seok Kyung HONG ; Byung Sang HAN ; Young Ho HONG ; Hoon KIM ; Chun Gil KIM
Korean Journal of Otolaryngology - Head and Neck Surgery 1993;36(3):372-380
No abstract available.
Tonsillectomy*
5.Experimental study of salivary gland stone fragmentation byextracorporeal shock wave lithotripsy.
Seok Kyung HONG ; Byung Sang HAN ; Hang PARK ; Young Ho HONG ; Hoon KIM ; Chun Gil KIM
Korean Journal of Otolaryngology - Head and Neck Surgery 1992;35(5):626-631
No abstract available.
Lithotripsy*
;
Salivary Gland Calculi*
;
Salivary Glands*
;
Shock*
7.Ichthysiform Skin Eruptions Possibly Due to Lovastatin(Mevacor).
Joon Hong PARK ; Kyung Seok OH ; Hye Jin LEE ; Kyu Uang WHANG
Korean Journal of Dermatology 1999;37(4):535-537
Lovastatin(Mevacor) belongs to a new generation of potent drugs for the treatment of hypercho- lesterolemia. It acts by competitive inhibition of 3-hydroxyl-3-methylglutaryl coenzyme A(HMG-CoA) reductase, diminishing in that way the cholesterol synthesis in the liver. HMG-CoA reductase inhibitors also affect cutaneous lipid content. This can induce cutaneous side effects such as eczernatous rashes or xerotic, ichthyosiform lesions due to abnormality in the barrier function in some patients. We report a 54-year-old male patient who developed ichthyosiform skin lesions under treatment with lovastatin(Mevacor) for hypercholesterolemia.
Cholesterol
;
Exanthema
;
Humans
;
Hydroxymethylglutaryl-CoA Reductase Inhibitors
;
Hypercholesterolemia
;
Liver
;
Lovastatin
;
Male
;
Middle Aged
;
Oxidoreductases
;
Skin*
8.Determination of DNA-DNA Hybridization Condition for Rapid Identification of Mycobacterium Species.
Yun Sop CHONG ; Sang Nae CHO ; Kyung Won LEE ; Hong Seok PARK
Journal of the Korean Society for Microbiology 1999;34(2):137-145
Rapid identification of Mycobacterium spp. isolated from patients is important with increased isolation of mycobacteria other than tubercle bacilli (MOTT). DNA-DNA hybridization with streptavidin-peroxidase and tetramethylbenzidine (TMB) color reaction method was recognized as a useful tool for identification of various species of mycobacteria. In this study, optimum condition of the test was determined. The optimal concentrations of tetramethylbenzidine dihydrochloride and hydrogen peroxide for streptavidin-horseradish peroxidase were 0.3-0.6 ug/ ml and 0.16 mM, respectively. The TMB stock solution was stable when prepared in methanol and the dilution of TBM stock solution in 10 mM sodium citrate-10 mM EDTA solution (pH 5.0) gave highest peroxidase-TMB activity. The suitable composition of hybridization solution consisted of 2 x SSC, 10% dextran sulfate, 50 ug/ml salmon DNA, 5 x Denhardt's solution, and 50% formamide. The 5-minute heating at 100C of test DNA prior to photobiotin labeling significantly increased the reaction. In conclusion, DNA-DNA hybridization method with streptavidin-peroxidase and TMB color reaction method may be useful for rapid identification of Mycobacterium spp. isolated from patients.
Dextran Sulfate
;
DNA
;
Edetic Acid
;
Heating
;
Hot Temperature
;
Humans
;
Hydrogen Peroxide
;
Methanol
;
Mycobacterium*
;
Peroxidase
;
Salmon
;
Sodium
9.Diagnostic Significance of Total Lactate Dehydrogenase(LD) and LD Isoenzyme Measurement in the Body Fluids.
Chang Ho JEON ; Eun Kyung BAE ; Seok Il HONG ; Chung Sook KIM ; Young Hyun LEE
Yeungnam University Journal of Medicine 1986;3(1):193-199
Body fluid Lactate dehydrogenase and its isoenzyme Measurement was performed in 132 patients: 8 cases with peritonitis, 21 cases with malignant ascites, 43 cases with liver cirrhosis, 48 cases with tuberculous pleuritis, 12 cases with malignant pleural effusion respectively. Body fluid protein and glucose contents, red blood cell counts, white blood cell counts, cytologic examination were also performed as a comparative study. The results were as follows: 1. Measurement of total LD and protein amount could differentiate between transudate and exudates in the ascitic fluids. 2. In the malignant exudate of ascites and pleural fluid, the activity of LD2 isoenzyme was statistically increased compared with that of inflammatory exudates and the activity of LD4 isoenzyme was also incereased compared with that of serum (P<0.05). 3. The inflammatory exudates of pleural fluid and ascites demonstrated the increase of LD5 isoenzyme activity statistically compared with that of serum and malignant exudates (P<0.05). 4. A difference of total LD activity between malignant ascites and inflammatory ascites was significant statistically, while this was not observed in the pleural exudate. 5. Total LD and LD5 isoenzyme activity didn't correlated with the number of white blood cells in the exudate.
Ascites
;
Ascitic Fluid
;
Body Fluids*
;
Erythrocyte Count
;
Exudates and Transudates
;
Glucose
;
Humans
;
L-Lactate Dehydrogenase
;
Lactic Acid*
;
Leukocyte Count
;
Leukocytes
;
Liver Cirrhosis
;
Peritonitis
;
Pleural Effusion, Malignant
;
Pleurisy
10.Statistical Analysis of Antimicrobial Susceptibility Tested on Various Clinical Isolates of Bacteria.
Eun Kyung BAE ; Chang Ho JEON ; Seok Il HONG ; Chung Sook KIM
Yeungnam University Journal of Medicine 1986;3(1):185-192
Antimicrobial susceptibility of the bacterial strains isolated from clinical specimens during the period from June, 1983 to June, 1986 in Yeungnam Medical Center was studied and the following results were obtained. 1. Staphylococcus aureus was highly susceptible to cephalothin and its susceptibility to methicillin was gradually reduced. 2. Streptococcus strains except enterococcus were generally susceptible to penicillin, while most enterococci were susceptible to only ampicillin. 3. Gram-negative rods including Escherichia coli were highly susceptible to amikacin and tobramycin. 4. Serratia were generally less susceptible to the amtimicrobials tested than other Enterobacteriaceae. Among them, Serratia marcescens showed the highest susceptibility to amikacin and chloramphenicol. 5. Pseudomonas aeruginosa revealed the highest susceptibility to amikacin and tobramycin and moderate susceptibility to carbenicillin and gentamycin. 6. Acinetobacter calcoaceticus revealed low susceptibility to most antimicrobials tested, showing only 30% susceptibility to amikacin, tobramycin and gentamycin in 1986.
Acinetobacter calcoaceticus
;
Amikacin
;
Ampicillin
;
Bacteria*
;
Carbenicillin
;
Cephalothin
;
Chloramphenicol
;
Enterobacteriaceae
;
Enterococcus
;
Escherichia coli
;
Gentamicins
;
Methicillin
;
Penicillins
;
Pseudomonas aeruginosa
;
Serratia
;
Serratia marcescens
;
Staphylococcus aureus
;
Streptococcus
;
Tobramycin