CD44v6 was known as tumor marker for tumor progression and metastasis in various kinds of carcinomas. The CD44v6 monoclonal antibody was produced by cell cultures or mouse ascite fluids using CD44v6 hybridoma cells, and its immunogloburin G (IgG) was purified by Protein A column. Using immobilized ficin and cysteine, the antibody fragment Fab was produced and purified by Protein A. Four CD44v6 scFv molecules were produced from the recombinant DNA and phage antibody technology and prurified by His-tag affinity chromatography. In order to inspect the function and specificity of each antibody molecule, western-blotting and ELISA against CD44v5-6 recombinant proteins and irnmunodetection in human ovarian carcinomas were estabilished. The results showed that immunodiagnosis did not distinguish the types of antibody fragments, but western-blotting and ELISA results did show some difference of their specificities and biological properties. These studies will contribute as a model study for the immunodiagnosis and therapy using the IgG, Fab and scFv of CD44v6 antibody to obtain the early detection of tumor progression and metastasis using immunoscintigraphy.
Animals ; Bacteriophages ; Cell Culture Techniques ; Chromatography, Affinity ; Cysteine ; DNA, Recombinant ; Enzyme-Linked Immunosorbent Assay ; Ficain ; Humans ; Hybridomas ; Immunoglobulin Fragments* ; Immunoglobulin G ; Immunologic Tests ; Mice ; Neoplasm Metastasis ; Recombinant Proteins ; Sensitivity and Specificity ; Staphylococcal Protein A

PURPOSE: This study was designed to evaluate the usefulness of a technetium-99m mercaptoacetyltriglycine (Tc-99m MAG3) single photon emission computed tomography (SPECT) performed on transplanted kidney. MATERIALS AND METHODS: Thirty renal transplant patients were included in this study. Planar scan was performed for 30 minutes using 555 MBq Tc-99m MAG3. A post-voiding SPECT scan was acquired on the third, seventh, fourteenth and twenty eighth day after transplantation. RESULTS: SPECT scan showed interpretable image quality in 26 of 30 patients (86.7%) and 84 in 120 scans (70%). Fourteen of 26 patients with interpretable SPECT image showed decreased or increased radioactivity, but only 5 had abnormal findings on the planar scan. Focal SPECT defects were seen in allografts with normal function (n=3), acute tubular necrosis (n=3), and acute rejection (n=2). The defects are thought to reflect focally underperfused renal parenchyme or, in normal allografts, an artifact from uneven radioactivity distribution. Four of 10 patients with renal arterial variation showed focally decreased radioactivity and SPECT helped guide further studies that confirmed the exact cause. Five of 10 patients with acute tubular necrosis or acute rejection showed focally decreased radioactivity, but its relation to the patients' clinical course was not clear. Focally increased radioactivity was observed in 5 allografts with normal function and 1 with double ureter in which local clearance delay was observed. CONCLUSION: Tc-99m MAG3 SPECT renal scan can detect additional focal abnormalities compared to planar scan. Further study is necessary to elucidate the exact clinical significance of the SPECT findings.
Allografts ; Artifacts ; Humans ; Kidney* ; Necrosis ; Radioactivity ; Tomography, Emission-Computed, Single-Photon* ; Ureter

Authors want to report that PTA was an out-standing therapeutic method in treatment of renovascular hypertension by renal artery stenosis due to Takaysu's arteritis in an 18-years-old Korean female. Abdominal aortogram and bilateral renal arteriogram showed mild smooth narrowing of the mid abdominal aorta of 4cm length from origin of renal arteries, complete occlusion of left renal artery from it's origin with a few small collateral arteries and severe stenosis of proximal right renal artery. PTA of right renal artery was performed with Gruntzign Balloon dialtation catheter, and the patient's blood pressure was down to 120/80 from 220/130,stable for 8 months follow-up examination. Takayasu's arteritis is one of the msot important cause of renovascular hypertension in Orenals. Multiple treatement modalities, such as steroid administration or variable surgical intervention, were attempted and limited response was presented. But PTA is a simple, cost-effective, repeated,and relatively complicaiton-free method in treatment of renovascular hypertension due ot Takayasu's arteritis, with an outstanding result, as authors case.
Angioplasty ; Aorta, Abdominal ; Arteries ; Arteritis ; Blood Pressure ; Catheters ; Constriction, Pathologic ; Female ; Follow-Up Studies ; Humans ; Hypertension, Renovascular ; Methods ; Renal Artery Obstruction ; Renal Artery ; Takayasu Arteritis

The pulmonary sequestration is rare congenital anomaly characterized by the presence of a part of lung tissuewhich is supplied by an aberrant systemic artery from the aorta or its branch and usually has no communicationwith the normal bronchial tree. The authors present 3 cases of intralobar pulmonary sequestration, studied by CTand /or US. Typical CT features of intralobar pulmonary sequestration are homogenous or unhomogeneous, relativelylow density lung mass near the diaphragm, having feeding artery from the aorta or branching vessels within themass during bolus injection or dynamic CT.
Aorta ; Arteries ; Bronchopulmonary Sequestration ; Diaphragm ; Lung ; Trees ; Ultrasonography

No abstract available.
Rhabdomyolysis* ; Technetium Tc 99m Medronate*

PURPOSE: We studied the diagnostic significance of bone scan in evaluation of bone metastasis by lung cancer, prevalence rate, and the causes of false positive bone scan and soft tissue accumulation of bone seeking agent. MATERIALS AND METHODS: The subject included 73 lung cancer patients with bone scan. We analyzed the frequency of bone metastasis, its distribution and configuration, and any relationship between bone pain and corresponding region on bone scan. The positive findings of bone scan were compared with simple X-ray film, CT, MRI and other diagnostic modalities. The false positive bone scan and the soft tissue accumulation of bone seeking agent were analized. RESULTS: The positive findings on bone scan were noted in 26 cases(36%) and they were coexistant with bone pain in 30%. The correspondance between bone scan and bone X-ray was 38%. False positive bone scans were seen in 12 cases(16%), which include fracture due to thoracotomy and trauma, degenerative bone diseases, and bifid rib. Accumulation of bone seeking agent in soft tissue were seen in 13 cases(18%), which included primary tumor, enlarged cervical lymph node, pleural effusion, ascites and pleural thickening. CONCLUSION: Bone scans should be carefully interpreted in detecting bone metastasis in primary malignancy, because of the 16% false positivity and 18% soft tissue accumulation rate. It is very important to note that the correlation between bone pain and positive findings of bone scans was only 38%.
Ascites ; Bone Diseases ; Humans ; Lung Neoplasms* ; Lung* ; Lymph Nodes ; Magnetic Resonance Imaging ; Neoplasm Metastasis ; Pleural Effusion ; Prevalence ; Ribs ; Thoracotomy ; X-Ray Film

PURPOSE: This study is designed to evaluate the usefulness of (99m)Tc-MAG3 renal scan in the diagnosis of early surgical complication of renal transplantation comparing with that of ultrasonography. METHODS: 203 renal transplantations, from January 2000 to December 2004, were studied retrospectively. (99m)Tc-MAG3 renal scan and ultrasonography were performed routinely for evaluation of allograft kidney at postoperative day 3, 7, 14 and 21 or 28 respectively. RESULTS: Thirteen early surgical complications (6.4%) from 203 recipients were developed during the first one month after transplantation. Six cases of urological complications were noticed. And six cases of hematoma and one case of lymphocele were also developed. (99m)Tc-MAG3 renal scan showed abnormality in ten cases (76.9%) of thirteen early surgical complications. (99m)Tc-MAG3 renal scan revealed all of six urologic complications (100%), and four of six hematomas (66.7%). But one lymphocele was not detected by (99m)Tc-MAG3 renal scan. Ultrasonography showed abnormal findings in eight cases of 13 early surgical complications (61.5%): one of four urine leakage (25%), two of two urinomas (100%), four of six hematomas (66.7%), and one lymphocele (100%). CONCLUSION: (99m)Tc-MAG3 renal scan is more sensitive than ultrasonography in detection of earlysurgical complications of renal transplantation. Then (99m)Tc-MAG3 renal scan is useful test for screening and follow-up of early surgical complications after renal transplantation.
Allografts ; Diagnosis ; Hematoma ; Kidney ; Kidney Transplantation ; Lymphocele ; Mass Screening ; Retrospective Studies ; Ultrasonography* ; Urinoma

No abstract available in English.
Arteriovenous Fistula* ; Heart Septal Defects, Ventricular*

No abstract available.
Adult* ; Humans ; Magnetic Resonance Imaging*

Intraventricular cysticercosis is the rest form of cerebral cysticercosis and almost all are within the fourthand third ventricles. Authors have experienced a case of pathologically proven giant intraventricular cysticercalcyst involving the lateral ventricle, studied by conventional CT and metrizamide CT, and report with brief reviewof literature.
Cysticercosis ; Lateral Ventricles ; Metrizamide ; Third Ventricle