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OBJECTIVE: Placental oxidative stress is known to be a factor that contributes to pregnancy failure. The aim of this study was to determine whether selenium could induce antioxidant gene expression and regulate invasive activity and mitochondrial activity in trophoblasts, which are a major cell type of the placenta. METHODS: To understand the effects of selenium on trophoblast cells exposed to hypoxia, the viability and invasive activity of trophoblasts were analyzed. The expression of antioxidant enzymes was assessed by reverse-transcription polymerase chain reaction. In addition, the effects of selenium treatment on mitochondrial activity were evaluated in terms of adenosine triphosphate production, mitochondrial membrane potential, and reactive oxygen species levels. RESULTS: Selenium showed positive effects on the viability and migration activity of trophoblast cells when exposed to hypoxia. Interestingly, the increased heme oxygenase 1 expression under hypoxic conditions was decreased by selenium treatment, whereas superoxide dismutase expression was increased in trophoblast cells by selenium treatment for 72 hours, regardless of hypoxia. Selenium-treated trophoblast cells showed increased mitochondrial membrane potential and decreased reactive oxygen species levels under hypoxic conditions for 72 hours. CONCLUSION: These results will be used as basic data for understanding the mechanism of how trophoblast cells respond to oxidative stress and how selenium promotes the upregulation of related genes and improves the survival rate and invasive ability of trophoblasts through regulating mitochondrial activity. These results suggest that selenium may be used in reproductive medicine for purposes including infertility treatment.
Adenosine Triphosphate ; Anoxia ; Antioxidants ; Gene Expression ; Heme Oxygenase-1 ; Infertility ; Membrane Potential, Mitochondrial ; Mitochondria ; Oxidative Stress ; Placenta ; Polymerase Chain Reaction ; Pregnancy ; Reactive Oxygen Species ; Reproductive Medicine ; Selenium* ; Superoxide Dismutase ; Survival Rate ; Trophoblasts* ; Up-Regulation

8.Antiendometrial antibodies in the serum of patients with endometriosis.

Jung Gu KIM ; Chi Seok AHN ; Byung Gu YOON ; Kyung Hee LEE ; Seok Hyun KIM ; Shin Yong MOON ; Jin Yong LEE ; Yoon Seok CHANG

Korean Journal of Obstetrics and Gynecology 1992;35(1):103-108

9.Studies on oocyte donation in IVF program.

Seok Hyun KIM ; Chang Jae SHIN ; Jung Gu KIM ; Shin Yong MOON ; Jin Yong LEE ; Yoon Seok CHANG

Korean Journal of Obstetrics and Gynecology 1991;34(4):559-570

10.Clinical significance of serum progesterone level on day of human chorionic gonadotropin injection following gonadotropin releasing hormone agonist combined superovulation.

Yong Sang SONG ; In Hwa ROH ; Seok Hyun KIM ; Jung Gu KIM ; Shin Yong MOON ; Jin Yong LEE ; Yoon Seok CHANG

Korean Journal of Obstetrics and Gynecology 1991;34(10):1416-1424

1.The efficacy of stellate ganglion block in the treatment of idiopathic sudden sensorineural hearing loss.

2.Comparison of outcome after controlled ovarian hyperstimulation with GnRH agonist(Lupron) and gonadotropins between previous poor responders and premature LH surge responders.

3.Radial motor nerve conduction study using surface electrode in normal adults.

4.Improvement of titration methods for porcine rotavirus, its serum neutralizing antibody and of virus isolation from feces.

5.A case of combined pregnancy following IVF-ET.

6.Comparison of superovulation characteristics and in vitro fertilization outcomes between short and long protocols using gonadotropinreleasing hormone agonist.

7.Effects of selenium on the survival and invasion of trophoblasts.

8.Antiendometrial antibodies in the serum of patients with endometriosis.

9.Studies on oocyte donation in IVF program.

10.Clinical significance of serum progesterone level on day of human chorionic gonadotropin injection following gonadotropin releasing hormone agonist combined superovulation.

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