1.Comparative Study of the Standard Plaque Assay with Solid-overlay and Immunofocus Assay for Varicella-zoster Virus Titration.
Hwa Kyung LEE ; Tong Seok JEONG
Journal of the Korean Society of Virology 2000;30(1):61-70
Standard plaque assay using agarose-overlay has long been used for titration of many infectious virus particle. Plaque assay for the titration of varicella-zoster virus and its live vaccine requires three intermittent agarose overlay to visualize plaques. Overall procedure of the assay takes at least nine days from virus inoculation and microbe contamination including fungi is frequently accompanied during incubation period. We studied whether an immunofocus assay in conjunction with peroxidase-mediated immunohistochemical reaction may replace the standard plaque assay for the virus titration by comparing the two methods. A linear relationship was observed between number of foci and virus dilution. The number of foci in a given dilution of virus appeared a little higher than counted plaques formed in standard plaque assay. Independent titration results obtained from two assay methods for a given dilution of virus demonstrated a strong correlation (r2=0.99). Foci of virus infected cells as revealed by the enzyme reaction could be counted either 4 days post-infection (p.i.) under low magnification (40X) microscopy, or 6 days p.i. by naked eye observation. Larger size of cell cuture plate, virus adsorption at 35 degrees C, and 10% FBS in diluent appeared to be better conditions for the assay. Immunofocus assay will be an effective and dependable titration method for varicella-zoster virus and its live vaccine in place of the standard plaque assay in respect to accuracy, costs, and experimental convenience.
Adsorption
;
Fungi
;
Herpesvirus 3, Human*
;
Microscopy
;
Sepharose
;
Virion
2.Chronic intestinal pseudoobstruction syndrome.
Kyung Mo YEON ; Jeong Kee SEO ; Yong Seok LEE
Journal of the Korean Radiological Society 1992;28(2):287-292
Chronic intestional pseudoobstruction syndrome is a rare clinical condition in which impaired intestinal peristalsis. Causes recurrent symptoms of bowel obstruction in the absence of a mechanical occlusion. This syndrome may involve variable segments of small or large bowel. And may be associated with urinary bladder retention. This study included 6 children(3 boys and 3 girls) of chronic intestinal obstruction. Four were symptomatic at birth and two were of the ages of one month and one year. All had abdominal distention and defication difficulty. Five had urinary bladder distention. Despite parenteral nutrition and surgical intervention(ileostomy or colostomy), bowel obstruction persisted and four patients expired from sepsis within one year. All had gaseous distention of small and large bowel on abdominal films. In small bowel series. Consistent findings were variable degree of dilatation. Decreased peristalsis(prolonged transit time) and microcolon or microrectum. This disease entity must be differentiated from congenital megacolon, ileal atresia and megacystis syndrome.
Dilatation
;
Hirschsprung Disease
;
Humans
;
Intestinal Obstruction
;
Intestinal Pseudo-Obstruction*
;
Parenteral Nutrition
;
Parturition
;
Peristalsis
;
Sepsis
;
Urinary Bladder
3.Two cases of huge cystic renal cell carcinoma.
Jun Seok PARK ; Jeong Zoo LEE ; Jong Byung YOON
Korean Journal of Nephrology 1992;11(3):291-296
No abstract available.
Carcinoma, Renal Cell*
4.Retraction note to: "Clinical Significance of the Axillary Arch in Sentinel Lymph Node Biopsy".
Won Ho KIL ; Jeong Eon LEE ; Seok Jin NAM
Journal of Breast Cancer 2015;18(1):101-101
All authors would like to withdraw the article because they have found a mistake in selecting subjects for this study.
5.Management of retroperitoneal hematoma.
Young Seok JEONG ; Jin Yong LEE ; Sang Hyo KIM
Journal of the Korean Surgical Society 1991;40(3):341-353
No abstract available.
Hematoma*
6.Evaluation of Child Health Information Articles in Newspapers.
Shin Jeong KIM ; Jeong Eun LEE ; Hwan Seok CHOI
Korean Journal of Child Health Nursing 1999;5(3):329-339
The purpose of this study was to take the right direction and meet the requirements of newspaper function about child health through evaluation of child health information articles in newspapers. Data were collected 4 main daily newspaper by selecting child health information articles during 1 year from January 1 to December 31, 1998. The results of this study are as follows. The frequency according to health category, disease treatment(47.7%) topped followed by health maintenance?promotion(28.8%), growth?development(12.1%), disease prevention(11.4%). The frequency according to WHO international disease classification, infectious disease (23.6%) take most. In evaluation area of child health information, practical usage(3.78) topped followed by accuracy(3.68), comprehensiveness(3.64), clearness (3.48), concreteness(3.33).
Child
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Child*
;
Classification
;
Communicable Diseases
;
Humans
;
Newspapers*
;
Child Health*
;
Periodicals
7.Evaluation of Child Health Information Articles in Newspapers.
Shin Jeong KIM ; Jeong Eun LEE ; Hwan Seok CHOI
Korean Journal of Child Health Nursing 1999;5(3):329-339
The purpose of this study was to take the right direction and meet the requirements of newspaper function about child health through evaluation of child health information articles in newspapers. Data were collected 4 main daily newspaper by selecting child health information articles during 1 year from January 1 to December 31, 1998. The results of this study are as follows. The frequency according to health category, disease treatment(47.7%) topped followed by health maintenance?promotion(28.8%), growth?development(12.1%), disease prevention(11.4%). The frequency according to WHO international disease classification, infectious disease (23.6%) take most. In evaluation area of child health information, practical usage(3.78) topped followed by accuracy(3.68), comprehensiveness(3.64), clearness (3.48), concreteness(3.33).
Child
;
Child*
;
Classification
;
Communicable Diseases
;
Humans
;
Newspapers*
;
Child Health*
;
Periodicals
8.Comparison of Diagnostic Performance between Interictal F-18-FDG PET and Ictal Tc-99m-HMPAO SPECT in Occipital Lobe Epilepsy.
Myung Chul LEE ; June Key CHUNG ; Dong Soo LEE ; Jae Min JEONG ; Sang Kun LEE ; Jeong Seok YEO ; Seok Ki KIM ; Joo Yong KIM
Korean Journal of Nuclear Medicine 1999;33(3):262-272
PURPOSE: Interictal F-18-fluorodeoxyglucose (FDG) PET and ictal Tc-99m-HMPAO SPECT are found to be useful in localizing epileptogenic zones in neocortical lateral temporal or frontal lobe epilepsy. We investigated whether interictal F-18-FDG PET or ictal Tc-99m-HMPAO SPECT was useful to find epileptogenic zones in occipital lobe epilepsy (OLE). MATERIALS AND METHODS: We reviewed patterns of hypometabolism in interictal F-18-FDG PET and of hyperperfusion in ictal Tc-99m-HMPAO SPECT in 17 OLE patients (mean age=27+/-6.8 year, M:F=10:7, injection time=30+/-17 sec). OLE was diagnosed based on invasive electroencephalography (EEG) study, surgery and post-surgical outcome (Engel class I in all for average 14 months). RESULTS: Epileptogenic zones were correctly localized in 9 (60%) out of 15 patients by interictal F-18-FDG PET. Epiletogenic hemispheres were correctly lateralized in 14 patients (93%). By ictal Tc-99m-HMPAO SPECT, epileptogenic hemispheres were correctly lateralized in 13 patients (76%), but localization was possible only in 3 patients (18%). Among patients who showed no abnormality with MR imaging and no correct localization with ictal Tc-99m-HMPAO SPECT, interictal F-18-FDG PET was helpful in 2 patients. CONCLUSION:: Ictal Tc-99m-HMPAO SPECT was helpful in lateralization but not in localization in OLE. Interictal F-18-FDG PET was helpful for localization of epileptogenic zones even in patients with ambiguous MR or ictal SPECT findings. Key KW: Occipital lobe epilepsy, Tc-99m-HMPAO, Ictal perfusion SPECT, F-18-FDG, PET, SPECT
Electroencephalography
;
Epilepsies, Partial*
;
Epilepsy, Frontal Lobe
;
Humans
;
Magnetic Resonance Imaging
;
Occipital Lobe*
;
Perfusion
;
Tomography, Emission-Computed, Single-Photon*
9.The Effects of Human Adipose Tissue-derived Stem Cells on Degenerative Change of Knee in Rabbit Model.
Ki Hwan JEONG ; Seok Kwun KIM ; Jae Oo JEONG ; Jeong HEO ; Yong Seok KWON ; Keun Cheol LEE
Journal of the Korean Society of Plastic and Reconstructive Surgeons 2008;35(6):637-644
PURPOSE: The survival of bone marrow derived stem cell was reported several times. But the survival of adipose tissue derived stem cells(hASCs) was not mentioned on. We studied the adipose tissue derived stem cell's survival and effect on articular cartilage in rabbits. METHODS: Osteoarthritis was induced in twenty New Zealand white rabbits by intraarticular injection of monosodium iodoacetate(MIA). After four weeks, hASCs were also injected into the knee joints space without any vehicle, but the control group received phosphate buffered saline only. The histologic grade of articular cartilage was measured in 4 and 8 weeks after the transplantation of hASC and the viability of injected stem cells measured by Fluorescent in situ Hybridization (FISH) examination. RESULTS: After 4 and 8 weeks from hASCs transplantation, histologic grade was not significantly difference between two groups(p>0.05), and the Y chromosome of the transplanted hASCs was not detected in articular cartilage. CONCLUSION: We found that direct injection of hASC in joint space didn't work on damaged articular cartilage repair.
Adipose Tissue
;
Bone Marrow
;
Cartilage, Articular
;
Humans
;
In Situ Hybridization, Fluorescence
;
Injections, Intra-Articular
;
Joints
;
Knee
;
Knee Joint
;
Osteoarthritis
;
Rabbits
;
Stem Cells
;
Transplants
;
Y Chromosome
10.Examination of Endoscopic Mucosal Resection of Early Gastric Cancer.
Seung Min LEE ; Seung Won SEO ; Jae Kyu SEONG ; Byung Seok LEE ; Hyun Yong JEONG
Korean Journal of Gastrointestinal Endoscopy 2002;24(3):129-134
BACKGROUND/AIMS: The endoscopic mucosal resection has now been adopted for a useful modality in both curative therapy and accurate diagnosis of early gastric cancer. A retrospective study was done to evaluate the indication and the limitation of endoscopic mucosal resection of early gastric cancer. METHODS: We studied 20 cases of early gastric cancer treated by endoscopic mucosal resection in Chungnam National University Hospital from November, 1995 to July, 1997. RESULTS: 1) The size affected the curability: 83. 3% of lesions less than 2 cm and 50. 0% of those larger than 2 cm were resected completely. 2) The depth of cancer invasion affected the curability: 87. 5% of lesion confined to mucosa were resected completely, while all of submucosal cancers were resected incompletely. 3) Among fourteen cases resected completely, three cases of lesions larger than 2 cm were residual cancer and two of those less than 2 cm were recurred locally. CONCLUSIONS: To be a curative therapy by endoscopic mucosal resection of early gastric cancer, we think that careful selection of the lesion, that is lesion less than 2cm and confined to mucosa, is necessary
Chungcheongnam-do
;
Diagnosis
;
Mucous Membrane
;
Neoplasm, Residual
;
Retrospective Studies
;
Stomach Neoplasms*