1.Essential Hypertension and renal role: Low renin hypertension.
Eun Seok KANG ; Sung Kyew KANG
Korean Journal of Medicine 2003;65(4):389-394
No abstract available.
Hypertension*
;
Renin*
2.Update on pathogenesis of uric acid nephrolithiasis.
Eun Seok KANG ; Kyung Pyo KANG ; Sung Kyew KANG
Korean Journal of Medicine 2006;70(1):4-10
No abstract available.
Nephrolithiasis*
;
Uric Acid*
3.A Case of Leukemia Cutis.
Eun Seok YANG ; Eun Young KWAK ; Kang Rhun KOO ; Kang Ho KIM ; Young Bong PARK
Journal of the Korean Pediatric Society 1990;33(2):246-251
No abstract available.
Leukemia*
4.Expression of Green Fluorescent Protein in Both Spodoptera frugiperda Cells and Bombyx mori Larvae by Ac-Bm Hybrid Virus.
Byung Rae JIN ; Hyung Joo YOON ; Eun Young YUN ; Seok Woo KANG ; Eun Sook CHO ; Seok Kwon KANG
Journal of the Korean Society of Virology 1998;28(3):225-232
We have expressed GFP in Sf9 and Bm5 cells or Bombyx by larvae by using Ac-Bm hybrid virus capable of replicating in both Bm5 and Sf9 cells. Genomic DNA of Ac-Bm hybrid virus expressing P-galactosidase was cotransfected with baculovirus transfer vector containing GFP gene, pBacPAK-GFP in Sf9 cells. The Ac-Bm hybrid virus harboring GFP was named as Ac-Bm hybrid virus-GFP. The Ac-Bm hybrid virus-GFP-infected insect cells were easily selected by detecting the emission of GFP from each well of cell culture dish on the UV illuminator. GFP produced by Ac-Bm hybrid virus-GFP in Sf9 and Bm5 cells or B. mori larvae was confirmed by SDS-PAGE and Western blot analysis using GFP antibody. In addition, B. mori larvae infected with Ac-Bm hybrid virus-GFP was apparently appeared fluorescence from the whole body at 5 days postinoculation. The fluorescence of GFP from the hemolymph and fat body of B. mori larvae infected with Ac-Bm hybrid virus-GFP was also observed by fluorescence microscope. In conclusion, our results demonstrated that in baculovirus expression vector system, use of Ac-Bm hybrid virus have an additional advantage of expanded host range for producing recombinant proteins.
Animals
;
Baculoviridae
;
Blotting, Western
;
Bombyx*
;
Cell Culture Techniques
;
DNA
;
Electrophoresis, Polyacrylamide Gel
;
Fat Body
;
Fluorescence
;
Hemolymph
;
Host Specificity
;
Insects
;
Larva*
;
Recombinant Proteins
;
Sf9 Cells
;
Spodoptera*
5.Etiology and Clinical Manifestations of Fever in Infants Younger than 3 Months Old: A Single Institution Study, 2008-2010.
Joon Young SEOK ; Ji Eun KANG ; Eun Young CHO ; Eun Hwa CHOI ; Hoan Jong LEE
Korean Journal of Pediatric Infectious Diseases 2012;19(3):121-130
PURPOSE: The purpose of this study is to investigate clinical features and causative organisms in febrile infants younger than three months, to help identification of high risk patients for serious bacterial infection (SBI). METHODS: A total of 313 febrile infants younger than three months, who had visited Seoul National University Children's Hospital from January 2008 to December 2010 were included. Clinical features, laboratory findings, causative organisms, and risk factors of SBI were analyzed by retrospective chart review. Causative bacterial or viral pathogens were identified by gram stain and cultures, rapid antigen tests, or the polymerase chain reaction from clinically reliable sources. RESULTS: Among 313 infants, etiologic organisms were identified in 127 cases (40.6%). Among 39 cases of bacterial infections, Escherichia coli (66.7%) and Streptococcus agalactiae (12.8%) were common. Enterovirus (33.7%), respiratory syncytial virus (19.8%), and rhinovirus (18.8%) were frequently detected in 88 cases of viral infection. Patients with SBI (39 cases) showed significantly higher values of the white blood cell count (14,473+/-6,824/mm3 vs. 11,254+/-5,775/mm3, P=0.002) and the C-reactive protein (6.32+/-8.51 mg/L vs. 1.28+/-2.35 mg/L, P<0.001) than those without SBI (274 cases). The clinical risk factors for SBI were the male (OR 3.7, 95% CI 1.5-8.9), the presence of neurologic symptoms (OR 4.8, 95% CI 1.4-16.8), and the absence of family members with respiratory symptoms (OR 3.6, 95% CI 1.2-11.3). CONCLUSION: This study identified common pathogens and risk factors for SBI in febrile infants younger than three months. These findings may be useful to guide management of febrile young infants.
Bacterial Infections
;
C-Reactive Protein
;
Enterovirus
;
Escherichia coli
;
Fever
;
Humans
;
Infant
;
Infant, Newborn
;
Leukocyte Count
;
Male
;
Neurologic Manifestations
;
Polymerase Chain Reaction
;
Respiratory Syncytial Viruses
;
Retrospective Studies
;
Rhinovirus
;
Risk Factors
;
Sepsis
;
Streptococcus agalactiae
6.Radial motor nerve conduction study using surface electrode in normal adults.
Sae Yoon KANG ; Young Jin KO ; Eun Seok CHOI ; Chan Seok OH
Journal of the Korean Academy of Rehabilitation Medicine 1992;16(4):385-389
No abstract available.
Adult*
;
Electrodes*
;
Humans
;
Neural Conduction*
7.Comparison of Clinical Outcomes according to the Catheters undergoing Intrauterine Insemination.
Hee Dong CHAE ; Eun Hee KANG ; Chung Hoon KIM ; Byung Moon KANG ; Yoon Seok CHANG
Korean Journal of Obstetrics and Gynecology 2000;43(2):254-259
OBJECTIVE: To compare the clinical outcomes of intrauterine insemination (IUI) according to the catheter used. MATERIALS AND METHOD: From March 1998 to September 1998, total 95 infertile patients were included in this study. Patients were randomly allocated to TomCat group (n = 39) and Mackler group (n = 56) according to the catheter for insemination. The controlled ovarian hyperstimulation (COH) using luteal long protocol of gonadotropin releasing hormone agonist (GnRH-a) was used in all patients. Statistical analysis was performed using Student's t-test, Fisher's exact test, and x2 test as appropriate. Statistical significance was defined as p < 0.05. RESULTS: The total dose and duration of exogeneous gonadotropin required were similar between the two groups. There were also no significant differences in serum estradiol (E2) level, endometrial thickness and texture on the day of hCG administration between the two groups. However, the percentage of uterine souding due to failure of initial approach was significantly higher in TomCat group compared to Mackler group (23.1% vs. 0%, p < 0.01). The percentage of bleeding after IUI in TomCat group seemed to be higher than that in Mackler group (15.4% vs. 3.6%, p = 0.06), although there was no statistically significant difference between the two groups. There was also no significant difference in the clinical pregnancy rate per patient between the two groups. CONCLUSION: These results suggested that using Mackler catheter might be effective for IUI, especially for the patients with cervical factor infertility.
Catheters*
;
Estradiol
;
Gonadotropin-Releasing Hormone
;
Gonadotropins
;
Hemorrhage
;
Humans
;
Infertility
;
Insemination*
;
Pregnancy Rate
8.Difference in Sexual Knowledge and Attitudes between High School and College Girls.
Seok Eun KANG ; Eun Ju SONG ; Eun Ju LIM
Journal of Korean Academy of Adult Nursing 2005;17(5):753-761
PURPOSE: This study was performed to identify the difference in attitudes and knowledge between high school and college girls regarding sexual education. METHOD: Data was collected from questionnaires given to 99 high school girls and 110 college girls in Seoul and Kyunggi-do. Data was analyzed using the SPSS 12.0 program. RESULT: In middle school, the time given to sexual education influenced the students present knowledge of sexual education. The sources of sex education were friends, seniors and mass media. The score for sexual knowledge in high school girls was 12.78 and for college girls 24.19. The field that obtained the highest score in territorial sexual knowledge was 'reproductive physiology' in the high school girls. There was little awareness of venereal disease, AIDS, contraception. Sexual attitudes among college girls was more open than that of high school girls. There was a positive correlation between sexual knowledge and attitudes(r=.535, p<.001). The significant difference of items were residence and sexual experience(p<.001). CONCLUSION: This study was shown that realistic knowledge teaching of venereal diseases, contraception and AIDS should be included in planning of sexual education programs. Specially, the groups had sexual experience and college girls in the dormitory will need more specific teaching.
Contraception
;
Education
;
Female*
;
Friends
;
Gyeonggi-do
;
Humans
;
Mass Media
;
Seoul
;
Sex Education
;
Sexually Transmitted Diseases
;
Surveys and Questionnaires
9.Evaluation of fetal heart rate response and fetal well-being using acoustic stimulation test to the fetus at term.
Cheol UM ; Yoo Seok CHUNG ; Eun Sil JHANG ; Kyung Seok KANG ; Sung Nam CHO ; Jong Deok KIM
Korean Journal of Obstetrics and Gynecology 1991;34(12):1663-1669
No abstract available.
Acoustic Stimulation*
;
Acoustics*
;
Female
;
Fetal Heart*
;
Fetus*
;
Heart Rate, Fetal*
;
Pregnancy
10.Atrial Flutter Conversion in Infants and Children Using Transesophageal Atrial Pacing.
Jae Kon KO ; Seoung Ho KIM ; Eun Jung BAE ; I Seok KANG ; Heung Jae LEE
Journal of the Korean Pediatric Society 1994;37(7):969-975
Atrial flutter is and infrequent, but potentially unstable tachyarrythmia that occurs in pediatric ages. Transesophageal atrial pacing was used for treatment of 10 episodes of atrial flutter in 7 patients. At the time of atrial flutter conversion, patients were 6 days to 14 years old. 6 patients had associated with congenital heart disease. The atrial cycle length of atrial flutter ranged from 140 to 280 msec with variable atrioventricular conduction. Transesophageal atrial pacing was performed using a bipolar 4 F transesophageal electrode catheter. Atrial flutter conversion was accomplished with stimulation bursts using about 5 seconds of stimuli, 10 msec in duration at 20 to 27 mA. Pacing cycle length was 45 to 110 msec less than the atrial cycle length of tachycardia in 6 episodes. But in a neonate, underdrive pacing converted atrial flutter to sinus rhythm. Conversion attempts were unsuccessful on 2 occasions. Transesophageal atrial pacing is a safe and effective, minimally invasive technique for treatment of atrial flutter in infants and children.
Adolescent
;
Atrial Flutter*
;
Catheters
;
Child*
;
Electrodes
;
Heart Defects, Congenital
;
Humans
;
Infant*
;
Infant, Newborn
;
Tachycardia