Objective To investigate correlations between peripheral benzodiazepine receptors(PBRs) in mitochondria and synaptosomes in the brain and platelet membrane of rats during aging and to explore the significance of the relationships.Methods Sprague-Dawley rats of both sexes were divided into 3-,12-and(24-month) groups.All animals were sacrificed by decapitation and the brains were immediately removed.Mitochondrial components from dissected cerebral cortex and synaptosomal fractions from dissected hippocampus were isolated by gradient centrifugation.The membrane of platelets from venous blood was prepared by the method of hypotonic haemolysis.The specific binding assay of the radioactive PBRs antagonist PK11195 to membrane was performed.(Results A significant) difference about PK11195 binding activity of mitochondria was observed among three groups(F=194.6,P

Objective To investigate and compare the special psychologic characteristics of the post-traumatic epilepsy patients and their spouses.Methods The complete clinical data of 244 patients and their spouses were retrospectively and constractively analyzed. There were two distinct groups because of the etiology: group A including 122 patients of post-traumatic epilepsy and their spouses, and group B including essential epilepsy. Symptom Checklist-90 (SCL-90), Hamilton Anxiety Scale (HAMA), Hamilton Depression Scale (HAMD) were used to evaluate the psychologic status of objects.Results All of the single-item score of SCL-90 were significantly higher than the China norm (P<0.01) in both groups, and the score in group A was higher than that in group B. But the characteristics of the abnormal data were diffierent in patient from in spouse. The scores of the post-traumatic patients were higher than that of the spouses in 3 items, and lower in 5 items. Specially the depression and anxiety emotion of the spouses were more obvious than the patients in group A (P<0.01).Conclusion Both the patient and spouse have the conspicuous psychosomatic disorder, and the appearance is diffierent from each other. The psychosomatic disorder of post-traumatic epilepsy patient and spouse is more conspicuous than the essential epilepsy.

Objective To analyze the clinical features and risk factors for epilepsy during the acute phase of aseptic meningitis. Methods 138 cases of aseptic meningitis with complete clinical data were retrospectively reviewed. Among them 21 had seizures, and 117 had not. All of them were inpatients in the same period after meningitis. Based on a standardized questionnaire, a database was established with Microsoft Access 2002 including 51 indexes and some subindexes. Univariate analysis and nonconditional multivariate stepwise logistic regression were carried out based on the summarization of the characters of clinical seizure. Results Fourteen cases (66.7%) manifested generalized seizures in those patients with epilepsy. Seventeen patients (80.1%) experienced epilepsy within 2 weeks after meningitis. The independent risk factors for seizures during the acute phase of aseptic meningitis were the increased contents of cerebrospinal fluid protein (OR=19.40), raised intracranial pressure (OR=8.848), and low-voltage activity in electroencephalogram (OR=5.039). Conclusions Generalized seizure is the major type of epilepsy induced by acute aseptic meningitis. Epilepsy occurred within 2 weeks in most patients with meningitis and seizures. High level of cerebrospinal fluid protein, raised intracranial pressure and low-voltage activity in electroencephalogram were the main predictive risk factors for epilepsy during the acute phase of aseptic meningitis.

Objective To evaluate the clinical features in patients with pre- or post-operation epilepsy, and to explore the relationship of secondary epilepsy and craniocerebral operations. Methods The patients involved in present study were admitted and underwent the craniocerebral operations in the General Hospital of PLA from Jun.1999 to Feb.2008. The clinical data of epilepsy occurred at pre- or post-operation were retrospectively analyzed. All the 186 patients were divided into the pre-operation epilepsy group(Group A, n=90 ) and the post-operation epilepsy group(Group B, n=96). Based on a standardized questionnaire, a database was established with Microsoft Access 2007 including the primary etiological factor, the location of epileptogenic focus, the seizure frequency, the seizure type, and the abnormal electroencephalogram (EEG). Results Intracranial tumor and cerebrovascular malformation were the main primary etiological factors in group A, and intracranial tumor, cerebral trauma, cerebrovascular malformation and acute cerebral apoplexy were the main primary etiological factors in group B. The four main primary etiological factors for seizures were discovered in significant difference between group A and group B(?2=45.857 3,P=0.000 0). Cranial computed tomography(CT) or nuclear magnetic resonance imaging(MRI) detected that the most locations of epileptogenic focus were in frontal lobe(31.8% in group A and 29.5% in group B), temporal lobe(30.7% in group A and 26.3% in group B), and parietal lobe(17.0% in group A and 24.2% in group B). The frequency of seizure was significantly decreased in group A(?2=11.313 3,P=0.010 1), and was increased in group B(?2=77.080 3,P=0.000 0). The quantity of abnormal EEG for focal epilepsy was significantly decreased in group A(?2=9.773 4,P=0.007 5), but the increasing quantity of abnormal EEG was not significant in group B(?2=4.366 0,P=0.112 7). No significant difference in seizure type was proved after the craniocerebral operations in group A(?2=0.214 3,P=0.643 4). Campared with the post-operation epilepsy group, the location of epileptogenic focus did not show significant distinction in the pre-operation epilepsy group(?2=1.772 2,P=0.777 6). Conclusions Craniocerebral operation is an effective therapy for the secondary epilepsy with certain epileptogenic focus, and it is one of the definite causes of secondary epilepsy.

Objective To analyze the correlations of D-dimer and the Global Registry of Acute Coronary Events(GRACE)score with long-term heart failure(HF)in patients with acute myocardial infarction(AMI).Methods A total of 398 patients with AMI were selected as research objects and divided into normal D-dimer group(n=309)and elevated D-dimer group(n=89)based on the D-dimer level.Cox proportional hazard model was used to analyze the risk factors for long-term HF in both groups.Time-dependent receiver operating characteristic(ROC)curve was used to analyze the values of D-dimer,GRACE score and their combination in predicting long-term HF.According to the GRACE score and D-dimer level,398 patients were divided into low-value group(181 patients with normal D-dimerand low GRACE score),high-value group(70 patients with elevated D-dimer and high GRACE score),and middle-value group(147 patients did not meet the conditions of the low-value and high-value groups).Kaplan-Meier method was used to analyze the occurrence of long-term HF in the three groups.Point-biserial analysis was used to analyze the correlation between elevated D-dimer and the occurrence of long-term HF.Results The number of patients with long-term HF in the elevated D-dimer group was 2.3 times of the normal group.D-dimer and GRACE score were independent risk factors for long-term HF in patients with AMI(P＜0.05).Both D-dimer and GRACE score had certain predictive values for the occurrence of HF at 5 years after AMI,but the predictive efficiency of GRACE score was better.The incidence of long-term HF in the high-value group was significantly higher than that in the low-value group and the middle-value group(P＜0.01).D-dimer was signif-icantly positively correlated with the occurrence of long-term HF after AMI(P＜0.001).Conclu-sion Both D-dimer and GRACE score are independent risk factors for long-term HF in patients with AMI,and the two indexes have certain predictive value for the occurrence of long-term HF.Patients with both elevated indicators are high-risk groups for long-term HF.

Objective To analyze the correlations of D-dimer and the Global Registry of Acute Coronary Events(GRACE)score with long-term heart failure(HF)in patients with acute myocardial infarction(AMI).Methods A total of 398 patients with AMI were selected as research objects and divided into normal D-dimer group(n=309)and elevated D-dimer group(n=89)based on the D-dimer level.Cox proportional hazard model was used to analyze the risk factors for long-term HF in both groups.Time-dependent receiver operating characteristic(ROC)curve was used to analyze the values of D-dimer,GRACE score and their combination in predicting long-term HF.According to the GRACE score and D-dimer level,398 patients were divided into low-value group(181 patients with normal D-dimerand low GRACE score),high-value group(70 patients with elevated D-dimer and high GRACE score),and middle-value group(147 patients did not meet the conditions of the low-value and high-value groups).Kaplan-Meier method was used to analyze the occurrence of long-term HF in the three groups.Point-biserial analysis was used to analyze the correlation between elevated D-dimer and the occurrence of long-term HF.Results The number of patients with long-term HF in the elevated D-dimer group was 2.3 times of the normal group.D-dimer and GRACE score were independent risk factors for long-term HF in patients with AMI(P＜0.05).Both D-dimer and GRACE score had certain predictive values for the occurrence of HF at 5 years after AMI,but the predictive efficiency of GRACE score was better.The incidence of long-term HF in the high-value group was significantly higher than that in the low-value group and the middle-value group(P＜0.01).D-dimer was signif-icantly positively correlated with the occurrence of long-term HF after AMI(P＜0.001).Conclu-sion Both D-dimer and GRACE score are independent risk factors for long-term HF in patients with AMI,and the two indexes have certain predictive value for the occurrence of long-term HF.Patients with both elevated indicators are high-risk groups for long-term HF.