Objective To analyze the clinical value of three-dimensional ultrasound in the diagnosis of pelvic floor dysfunction disease.Methods From June 2016 to December 2017,95 cases of female pelvic floor dysfunction disease in the People's Hospital of Dongyang were selected as observation group.And 90 healthy women received physical examination during the same period were selected as control group.Both two groups were subjected to three-dimensional ultrasonography,and the results were analyzed.Results In the observation group,the anteroposterior diameter and area in resting period,anteroposterior diameter and area in tension period,anteroposterior diameter and area in anus period of the anal pelvic diaphragmatic hiatus were (65.58 ± 6.38)cm,(20.01 ± 3.98) cm2,(68.40 ± 5.54) cm,(22.50 ± 3.12) cm2,(60.17 ± 5.55) cm and (19.28 ± 2.52) cm2,respectively,which were significantly higher than those in the control group [(51.84 ± 3.46) cm,(13.93 ± 2.09) cm2,(53.65 ± 4.23) cm,(14.60 ± 2.95) cm2,(48.95 ± 3.48) cm,(13.45 ± 1.76) cm2] (t =18.066,12.903,20.272,13.200,16.369,18.150,P =0.000,0.000,0.000,0.000,0.000,and 0.000).But the pubic rectal muscle thickness of resting period,tension period,anal contraction period in the observation group were (10.28 ± 1.96) cm,(10.63 ± 1.57) cm,and (10.01 ± 1.13) cm,respectively,which were lower than those in the control group [(10.28 ± 1.96)cm,(10.63 ±1.57)cm,(10.35 ±1.49)cm] (t =1.015,1.940,1.741,P =0.311,0.053,0.083).The observation group was asymmetrical,asymmetrical on both sides and interrupted continuously.In the control group,the echo was uniform,bilateral symmetry,without interruption.Conclusion The use of three-dimensional ultrasound in the diagnosis of female pelvic floor dysfunction disease has good clinical value.