Objective:To study the effects of Taoist cognitive therapy on type A behavior of patients with coronary heart disease.Methods:206 patients with coronary heart disease were randomized divided into two groups. The study group received Taoist cognitive therapy for 3 months in addition to basic medical treatment.The control group was treated with medicine only.Both groups were followed for 6 months and evaluated with psychological scales,including Type A behavior scale,mental detachment scale.Clinical efficacy was also evaluated.Results:The score of type A had significant decreased from 25.8?7.6 to 20.8?5.8 after 3 months in the study group,the score of mental detachment significantly increased from 28.7?4.4 to 31.8?3.8(P

OBJECTIVETo investigate the influence of pleiotrophin (PTN) on the growth of rat hepatocytes.

METHODSPrimary rat hepatocytes were isolated from male Sprague-Dawley rats and divided into three groups: group A (negative control), cultivated in normal culture medium; group B (positive control), cultivated with culture medium supplemented with supernatant from the embryonic fibroblast 3T3 cell line; group C (experimental), cultivated with culture medium supplemented with human recombinant (hr) PTN (100 ng/ml). The hepatocytes' growth rate and level of secreted albumin (ALB) were evaluated by microscopy and biochemical assay, respectively. Significance of between-group differences were assessed by one-way ANOVA, and pairwise comparisons were performed by the least significant difference test.

RESULTSThe growth rates of hepatocytes in groups A, B and C were 2.800+/-0.084%, 4.300+/-0.132% and 3.800+/-0.053%, respectively. The growth rate of group B was significantly higher than the other two groups (F = 333.735, P less than 0.05). For all groups, the highest levels of secreted ALB were detected between the second and sixth day of culture, with g/L concentrations at day 2, 4 and 6 of: group A, 0.550+/-0.010, 0.900+/-0.030 and 0.300+/-0.040; group B, 0.900+/-0.030, 1.300+/-0.020 and 1.400+/-0.030; group C, 0.900+/-0.010, 1.160+/-0.010 and 0.700+/-0.050. The secreted ALB of group B was significantly higher than that of the other two groups (F = 651.355, 338.831 and 863.205, P less than 0.05 ).

CONCLUSIONPTN can benefit in vitro culturing of rat hepatocytes by stimulating growth and enhancing their ability to secrete albumin.

Albumins ; secretion ; Animals ; Carrier Proteins ; pharmacology ; Cells, Cultured ; Cytokines ; pharmacology ; Hepatocytes ; cytology ; drug effects ; secretion ; Male ; Rats ; Rats, Sprague-Dawley

This paper presents a computer-aided diagnosis method for prostate cancer detection using Trans-rectal ultrasound(TRUS) images. Firstly, statistical texture analysis is implemented in every ROI in segmented prostate images. From each ROI, grey level difference vector features, edge-frequency features and texture features in frequency domain are constructed. Then, the number of features is reduced using ANOVA statistics to select the optimal feature subset. Finally, SVM is applied to the selected subset for detecting the cancer regions. Experimental results show that the proposed algorithm can recognize and detect the cancer images effectively so as to supply essential information for a diagnosis.
Algorithms ; Artificial Intelligence ; Humans ; Image Interpretation, Computer-Assisted ; methods ; Male ; Neural Networks (Computer) ; Prostatic Neoplasms ; diagnostic imaging ; Signal Processing, Computer-Assisted ; Ultrasonography

In this paper, we propose that, the need of the costly re-initialization procedure can be completely eliminated by using the variation formulation, thus increasing the speed of computing operations. The edge detecting function in the geodesic active contour model is improved by incorporating a prior knowledge. The accuracy of the segmentation algorithm can be enhanced by using the minimal variance. Experimental results show that the proposed algorithm can segment the prostate ultrasound images effectively and avoid the problem of contour leakage.
Algorithms ; Humans ; Image Interpretation, Computer-Assisted ; methods ; Male ; Pattern Recognition, Automated ; methods ; Prostate ; diagnostic imaging ; Ultrasonography ; instrumentation ; methods

This paper proposes an algorithm of evaluating the compression depth, and then to extract four normalized mammary elasticity characteristic parameters with respect to the compression depth. The classification experiments show that these elasticity parameters have a good capability in determining whether the tumor is benign or malignant, and if combined with morphological parameters, the accuracy, sensitivity and specificity can be improved and increased to 95.19%, 98.82% and 92.16%, respectively.
Algorithms ; Breast Neoplasms ; diagnostic imaging ; Elasticity Imaging Techniques ; Female ; Humans ; Sensitivity and Specificity ; Ultrasonography, Mammary ; methods

Objective To observe the expression of human epididymis protein 4 (HE4) and carbohydrate antigen 125 (CA125) in endometrial carcinoma(EC) tissues,and to explore the relationship between the expression of HE4 and CA125 and the occurrence and development of EC.Methods The EC and paracancerous tissue specimens of 35 EC patients were selected from December 2015 to December 2016 in the First People's Hospital of Yibin City.The expression of HE4 and CA125 in EC tissues and paracancerous tissues was detected by immunohistochemistry.The relationship between the expression of HE4,CA125 and the clinicopathological features of EC was analyzed;and the correlation between HE4 and CA125 was analyzed too.Results The positive expression rates of HE4 and CA125 in EC tissues were 82.9％ (29/35) and 74.3％ (26/35);they were 11.4％ (4/35) and 14.3％ (5/35) in paracancerous tissues respectively;the positive expression rates of HE4and CA125 in EC tissues were significantly higher than those in paracancerous tissues(x2 =35.831,25.533;P ＜0.05).The positive expression rates of HE4 and CA125 in stage Ⅲ and Ⅳ EC tissues were 100.0％ (20/20) and 90.0％ (18/20);they were 60.0％ (9/15) and 53.3％ (8/15) in stage Ⅰ and Ⅱ EC tissues respectively;the positive expression rates of HE4 and CA125 in stage Ⅲ and Ⅳ EC tissues were significantly higher than those in stage Ⅰ and Ⅱ EC tissues(x2 =9.655,4.266;P ＜ 0.05).The positive expression rates of HE4 and CA125 in medium and low differentiated EC tissues were 100.0％ (18/18) and 83.3％ (15/18);they were 64.7％ (11/17) and 64.7％ (11/17) in highly differentiated EC tissues respectively;the positive expression rates of HE4 and CA125 in medium and low differentiated EC tissues were significantly higher than those in highly differentiated EC tissues(x2 =7.667,4.137;P ＜0.05).The positive expression rates of HE4 and CA125 in EC tissues with myometrial invasion depth≥ 1/2 were 100.0％ (18/18) and 94.4％ (17/18);they were 64.7％ (11/17) and 52.9％(9/17) in EC tissues with myometrial invasion depth ＜ 1/2 respectively;the positive expression rates of HE4 and CA125 in EC tissues with myometrial invasion depth ≥ 1/2 were significandy higher than those in EC tissues with myometrial invasion depth ＜ 1/2(x2 =7.667,7.884;P ＜ 0.05).The positive expression rates of HE4 and CA125 in EC tissues with lymph node metastasis were 100.0％ (19/19) and 78.9％ (15/19);they were 62.5％ (10/16) and 68.8％ (11/16) in EC tissues without lymph node metastasis respectively;the positive expression rate of HE4 in EC tissues with lymph node metastasis was significantly higher than that in EC tissues without lymph node metastasis (x2 =8.599,P ＜ 0.05),but there was no significant difference in the positive expression rate of CA125 in EC tissues with lymph node metastasis and without lymph node metastasis (x2 =0.473,P ＜0.05).The expression of HE4 and CA125 in EC tissues was not related to age,tumor size and pathological type(P ＜ 0.05).The expression of HE4 in EC tissues was positively correlated with the expression of CA125 (r =0.608,P ＜0.05).Conclusion HE4 and CA125 may be involved in the development and development of EC.The expression of HE4 and CA125 is high in EC tissues,the expression level of them is closely related to clinical stage,histological differentiation and myometrial invasion depth of EC.The high expression of HE4 was also related to lymph node metastasis.

Obejective To conduct fast identification analysis on chemical constituents of Mori Folium UPLC-Q-TOF/MS method.Methods ACQUITY UPLC-Q-TOF/MS spectrometer was used, acetonitrile and water (containing 0.1％ formic acid) as mobile phase with gradient elution. The flow rate was 0.3 mL/min, with ESI negative ion mode detection, data analysis with Masslynx4.1 software.Results According to the retention time provided by the mass spectrometry, the exact molecular weight and the secondary mass spectrometry were used to analyze the fragments by referring to literature, and 19 chemical constituents of Mori Folium were identified and deduced.Conclusion The method can analyze the chemical constituents of Mori Folium rapidly, sensitively and comprehensively, and provide the basis for the study of the pharmacological basis of Mori Folium.

Cardiovascular Diseases ; Humans ; RNA, Circular ; RNA, Long Noncoding

This study was to purposed to explore the methylation status changes of IEX-1 gene promoter CpG island and its relevance with occurrence of hematologic malignancies. The methylation status of IEX-1 gene promoter CpG island in 9 NB4, HL-60 U937, Raji, CA46, Jurkat, K562, CEM and Molt4 hematologic malignant cell lines was detected by using methylation-specific PCR, the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells treated by M. sssI enzyme and the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells untreated were used as positive and negative controls respectively. The results showed that the hypermethylation of IEX-1 gene promoter CpG islands was detected in NB4, Molt4 and Raji cell lines, as well as in normal peripheral blood mononuclear cells treated by M. sssl enzyme; the partial methylation status was found in CA46, CEM, U937, K562, HL-60 and Jurkat cell lines; the unmethylation status was observed in untreated normal peripheral blood mononuclear cells. It is concluded that the changes of methylation status of gene IEX-1 promoter CpG island correlates with hematologic malignancies to a certain extent.
Apoptosis Regulatory Proteins ; genetics ; Cell Line, Tumor ; CpG Islands ; DNA Methylation ; Gene Expression Regulation, Neoplastic ; Hematologic Neoplasms ; genetics ; pathology ; Humans ; Leukocytes, Mononuclear ; pathology ; Membrane Proteins ; genetics ; Promoter Regions, Genetic

OBJECTIVETo determine the value of concurrent chemoradiotherapy in patients with locoregionally advanced nasopharyngeal carcinoma from the mainland of China.

METHODSData were extracted from randomized trials comparing chemotherapy plus radiotherapy with radiotherapy alone in locally advanced nasopharyngeal carcinoma. Actuarial rates of survival and distant metastases were calculated. The followed electronic databases were searched the Chinese Biomedicine database, Pubmed, Medline, Embase and Cochrane library; Data were extracted by tow reviewers and Review manager 4.1 software was applied for statistical analysis.

RESULTSEighteen trials with 1993 patients were include according to the including criterion. The 3-year overall survival rate of the chemoradiotherapy group and the radiotherapy group were 68.47% and 56.38% respectively, and the 5-year overall survival rate of the two groups above were 51.91% and 41.09% respectively, while the distant metastases rate of the chemoradiotherapy group and the radiotherapy group were 26.19% and 38.71% respectively. The result demonstrated that chemoradiotherapy increased overall survival by 12% at 3 years, and 11% at 5 years after treatment. After chemoradiotherapy, the rate of distant metastasis was reduce by 12%.

CONCLUSIONSIn patients with locoregionally advanced nasopharyngeal carcinoma, chemoradiotherapy significantly improves overall survival at 3 years, and 5 years compared with radiotherapy alone.

Antineoplastic Combined Chemotherapy Protocols ; China ; Combined Modality Therapy ; Humans ; Nasopharyngeal Neoplasms ; drug therapy ; radiotherapy ; Radiotherapy ; Randomized Controlled Trials as Topic ; Survival Rate