Objective: To develop an HPLC method for determination of Isocorydine in Jinshining Eye gelatin. Method: AgiLentTC-C18 chromatographi coLumn(4.6 min×150 mm,5 μm) was used,methanoL-0.2% phosphoric acid soLution (adjust the pH 7.0 take methyL aLcohoL)(54:46)as the mobiLe phase, the detection waveLength was 267 nm,flow rate at 1.0 mL· min-1. Result: The calibration curve of Isocorydine as linear between 0.123 0～1.107 0μg, The average reeovery and the relative standard deviation were 97.11% and 1.65%. Conclusions: The method is simple, accurate, repeatable. It can be used for quality control of JinshiLe Eye gelatin.

AIM: To investigate the changes of serum angiogenic factors including vascular endothelial growth factor (VEGF) and basic fibroblast growth factor ( bFGF) in Mongolia patients with diabetic retinopathy ( DR) and to explore their roles in the progress of DR.
METHODS: Eighty - three patients with diabetes were divided into three groups according to the retinopathy, no DR group ( NDR, 25 cases), background DR ( NPDR, 31 cases) and proliferative DR group (PDR, 27 cases), and 36 age - matched healthy people ( control group) were selected. Serum concentrations of VEGF and bFGF were measured using ELISA method.
RESULTS: In Mongolia patients with diabetes, the serum concentrations of VEGF and bFGF were higher than those in control group; the serum concentrations of VEGF and bFGF in PDR group was higher than those of NDR and NPDR group (P< 0. 05); the concentrations of VEGF and bFGF in NPDR group was higher than that of NDR group (P<0. 05).
CONCLUSION: High level of VEGF and bFGF may be the important pathogenic factors for DR in Mongolia patients.

Objective To explore the effects of galantamine on function of learning and memory and cholinergic neurons in cortex and hippocampus in rat models of Alzheimer's disease (AD) so as to further investigate the mechanism of cholinesterase inhibitors in improving the cognitive ability of AD patients. Methods The AD rat models established by injecting amyloid-beta protein (A? 1-40) into the bilateral Meynert nucleus were intervened with galantamine, and their abilities of learning and memory were measured with a behavioral test. Meanwhile, the changes in choline acetyltransferase (ChAT) in the rats were examined with immunohistochemical technique. Results In the AD model rats administered with galantamine, the performances in the test were significantly improved and ChAT immunoreactive positive neurons were increased significantly in hippocampus and cortex fields. Conclusion Galantamine can significantly improve the function of learning and memory of the AD model rats, and enhancing the survival characteristics of cholinergic neurons may be one of the important mechanisms, indicating a new possible mechanism of cholinesterase inhibitors as a remedy for AD.

Primary intrahepatic cholangiocarcinoma (ICC) is the second frequent malignant tumor in adult liver,and appears an increasing tendency worldwide.Gross type is frequently mass-forming and a tubular adenocarcinoma is shown as the typical histopathological appearance.Surgical resection is the only curative treatment,and liver transplantation is selected for the patients with early ICC.Rediofrequency ablation,transcatheter arterial chemoembolization or molecular targeted therapies should be considered in the treatment of the unresectable or recurrent patients.

OBJECTIVE: Because of the widely origin, easily isolation and cultivation, the powerful multiplication ability and some other characteristics, endothelial progenitor cells have become the most important seeding cells in tissue-engineered vascular, but the condition to become a vascular in vitro, characteristics of endothelial progenitor cells and their progress in research and application in the tissue-engineered vascular deserve further study. DATA SOURCES: A computer-based online search of PUBMED database was undertaken to identify articles about endothelial progenitor cells being seeding cells in tissue-engineered vascular application published from January 1997 to July 2006 by using the keywords of "Endothelial progenitor cells,Tissue-engineered vascular,Tissue Engineering Blood Vessel" in English. STUDY SELECTION: The data were selected firstly, and quotation of each articles were read. Inclusive criteria: all articles related to the characteristics of endothelial progenitor cells and their progress in research and application in the tissue-engineered vascular were selected. Exclusive criteria: repetitive research or Meta articles were excluded. DATA EXTRACTION:Totally 136 articles were collected, of which 30 articles were associated with inclusive criteria. The 106 excluded articles were with old content or repetitive research. The 30 articles contained 8 articles on characteristics of endothelial progenitor cells, 14 articles about the advantages of endothelial progenitor cells as seeding cells in tissue-engineered vascular application, 8 articles on the application of endothelial progenitor cells construction in tissue-engineered vascular. DATA SYNTHESIS: ① Endothelial progenitor cells could be isolated from bone-marrow, peripheral blood, spleen and so on. They expressed CD133 and distinction from endothelial cells by CD133. Endothelial progenitor cells could transform to endothelial cells and smooth muscles, too. ② Endothelial progenitor cells could raise the density of capillary and the number of angiovascular. There were many research indicated that endothelial progenitor cells could improve ischemic, repair the injure of endocardium; Endothelial progenitor cells could improve reendothelialization, protect endomembrane, and hold the integrity of endomembrane; when incorporated with ischemic vascular, endothelial progenitor cells could improve patency rate and prevent restenosis. ③ With smooth muscle cells and some other materials, endothelial progenitor cells could widely applied in constructing tissue-engineered vascular and have a brilliant prospect in the tissue-engineering blood vessel. CONCLUSION: The research of endothelial progenitor cells will have a breakthrough in the expression of its vessel and the function in the organization remodeling. As one of the seeding cells, endothelial progenitor cells have widely origin and the powerful multiplication ability, and they have a brilliant prospect in the tissue-engineering blood vessel.

Objective To explore predictive value of baseline red cell distribution width (RDW) determined in a routine blood count for severity of chronic heart failure (CHF) and mortality during hospitalization.Methods The study group comprised 135 patients hospitalized in the our hospital with CHF in the Ⅱ ～ Ⅳ class according to New York Heart Association (NYHA).The control group included 44 patients in class Ⅰ.Hematological and biochemical parameters were obtained and compared among groups.Receiver operator characteristic (ROC) curves were used for assessing predictive values.Results RDW increased significantly in class Ⅲ and Ⅳ compared to class Ⅰ [(14.3 ±2.3)％ and (14.3 ± 1.7)％ vs (12.9 ±0.8)％,P ＜0.01].Areas under ROCs (AUCs) of RDW,N-terminal prohormone brain natriuretic peptide (NT-proBNP) for class Ⅳ HF was 0.817 and 0.840,respectively.RDW was significantly elevated in mortality group compared to survival group [(15.8 ± 1.8)％ vs (13.7 ± 1.7) ％,P ＜ 0.01],predictive value of RDW was lower than that of NT-proBNP but was comparable to white blood cell (WBC),neutrophil (NEU),lymphocyte (L),and neutrophil/lymphocyte ratio (N/L) for mortality during hospitalization with AUCs (0.837,0.939,0.858,0.891,0.885,and 0.885,respectively).RDW and NT-proBNP showed low predictive values for repeated admission (≥3).RDW was an independent risk factor for mortality (OR =2.531,95％ CI:1.371 ～4.671).Conclusions RDW increased significantly in class Ⅲ and Ⅳ patients and death group.The predictive value of RDW is comparable to NT-proBNP for class Ⅳ and lower than that of NT-proBNP for mortality.Elevated RDW is an independent risk factor for mortality.

Objective To compare the therapeutic efficacy of 125Iodine seed implantation and gamma knife in treating portal vein tumor thrombosis (PVTT) in hepatocellular carcinoma (HCC), and to discuss their clinical applications. Methods Between January 2009 and May 2013, a total of 60 HCC patients associated with PVTT were encountered at authors’ hospital. The patients were divided into gamma knife group (n=30) and 125Iodine seed group (n = 30). Gamma knife treatment was carried out for the patients of gamma knife group. A total of 3 - 10 target points were designed. Single dose of 3 - 6 Gy per fraction was used and 2 - 5 times radiotherapy every week were conducted. The total treatment dose was 30 - 50 Gy. In designing the therapeutic scheme, 50%-70%of iso-dose curve contained PTV. 125Iodine seed implantation was performed for the patients of 125Iodine seed group. After the treatment, the change of PVTT size was assessed. The clinical efficacy was evaluated. Results Three months after the treatment, in gamma knife group complete remission (CR) was seen in 0 patient (0%), partial remission (PR) in 8 patient (26.6%), stable disease (SD) in 17 patients (56.7%) and progressive disease (PD) in 5 patients (16.7%). In 125Iodine seed group, CR was obtained in 3 patients (10.0%), PR in 19 patients (63.4%), SD in 7 patients (23.3%) and PD in one patient (3.3%). Statistically significant differences in responding the therapy existed between the two groups (P < 0.05), although no significant difference in the improvement of ascites and hepatic function existed between the two groups (P<0.05). Conclusion For the treatment of portal vein tumor thrombosis of hepatocellular carcinoma, the short-term efficacy of 125Iodine seed implantation is much better than that of gamma knife, although its long-term effect needs to be further studied.

Infectious endophthalmitis refers to the acute suppurative inflammation of the uveal and retinal, with acute onset, severe symptoms, and poor prognosis.Early diagnosis and effective treatment is particularly important.Based on the way of intraocular infection, endophthalmitis can be divided into endogenous endophthalmitis and exogenous endophthalmitis.In the East Asian, liver abscess is the main source of endogenous endophthalmitis, and Klebsiella pneumoniae is the main pathogen.Liver abscess endophthalmitis is a serious cause of blindness caused by intraocular infection.A liver infection disseminated through hematogenous and the primary focus was hidden, which makes the disease was easy to be confused with immune related uveitis, causing high misdiagnosis rate, delaying the best time for diagnosis and treatment.In this article we reviewed the etiology, clinical features, diagnosis and treatment and prognosis of endogenous endophthalmitis caused by liver abscess.Clinicians should maintain a high index of suspicion to the endogenous endophthalmitis caused by liver abscess.

Objective To investigate the expression of NF-κB/p65 in nasal NK/T cell lymphomas. Methods Immunohistochemis-try and TUNEL were used to study the expression of NF-KB/p65 and cell apoptosis in 23 nasal NK/T cell lymphoma samples and 14 benign lymph node lesions. Results The NF-KB/p65 positive rates were 43.5% (10/23). The expression of NF-κB/p65 was negative correlated with apoptotic index among 23 nasal NK/T cell lymphomas(P <0. 05). The mean survival period in patients expressed NF-KB/p65 was sig-nificantly shorter than that in negative group(P <0. 05). Conclusions Apoptosis inhibited by overexpression of NF-κB/p65 might be in-volved in the development of nasal NK/T cell lymphoma. NF-κB/p65 expression may be an unfavorable prognostic factor of nasal NK/T cell lymphoma.

BACKGROUND: Recent studies have suggested that conversion from cyclosporine A (CsA) to tacrolimus (FK 506)-based regimen can improve renal allograft function and survival rate. But little is known about whether the conversion from CsA to tacrolimus(FK 506) plus mycophenolate mofetil (MMF)-based regimen exhibits the same or similar clinical efficacy. OBJECTIVE: To investigate the clinical efficacy and safety of converting CsA to FK506 plus MMF in treatment of different types of chronic allograft nephropathy (CAN). DESIGN, TIME AND SETTING: An observational and controlled trial was performed at the Center for Organ Transplantation, Zhujiang Hospital, Southern Medical University from January 2005 to October 2007. PARTICIPANTS: Fifteen-nine enrolled patients received CsA-based regimen after renal allografting. Following pathological confirm and typing, all patients were assigned to two groups: CAN with chronic rejection (CR, n = 31) and CAN without chronic rejection (non-CR, n = 28). FK 56 was purchased from Fujisawa Pharmaceutical Company, Ltd., Japan. MMF was sourced from Shanghai Roche Pharmaceutical Co., Ltd., China. METHODS: When patients were diagnosed CAN, the CsA regimen was conversed to FK506 plus MMF regimen. FK506 initiated at a dose of 0.08 mg/kg per day and then was adjusted to achieve steady-state whole blood trough levels of approximately 5-8 μg/L. MMF was used at a fixed dosage, 1.0 g/d, twice a day, only if relative adverse events occurred. All patients were followed up at least 6 months. MAIN OUTCOME MEASURES: Serum creatinine(Scr), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), 24-h proteinuria, glomerular filtration rate (GFR), and complications. RESULTS: All initial 59 patients were included in the final analysis. At 6 months after regimen conversion, the levels of Scr, TC, TG, LDL, and 24-hour proteinuria were significantly reduced in non-CR, in particular CR, groups, compared with prior to conversion (P< 0.05). GFR was markedly increased in both the CR and non-CR groups (P< 0.05). In the CR group, 20 patients obtained improved results, 7 got stable results, and 4 showed ineffective results. The effective rate of regimen conversion was 64.5% and 32.1% in the CR and non-CR groups, respectively, and significant difference existed between the two groups (P < 0.05). Compared with prior to conversion, the incidence of hypertension and hyperlipemia was significantly decreased after regimen conversion (P< 0.05). There was no significant difference in diabetes mellitus, opportunistic infection, and malignancy between prior to and after regimen conversion. CONCLUSION: FK506 plus MMF-based regimen can markedly improve the function of renal graft of CAN, in particular CR, patients.