Arthroscopy ; instrumentation ; Humans ; Knee Injuries ; diagnosis ; pathology ; Magnetic Resonance Imaging ; instrumentation ; Male ; Menisci, Tibial ; surgery ; Middle Aged ; Surgical Flaps ; Tibial Meniscus Injuries

Acute Disease ; Adult ; Humans ; Hydrogen Sulfide ; poisoning ; Male ; Poisoning ; pathology

Heart Defects, Congenital ; diagnosis ; therapy ; Humans ; Infant, Newborn

1 303 syphilis patients had seen their doctors at the STD clinic of our hospital in 2012 . All first-visit syphilis patients were screened for HIV. The HIV-infected individuals were tested for syphilis. 8 cases were con-firmed to be HIV-infected individual, which constituted 0. 6% of syphilis cases. 4 cases were male and 4 cases were female among 8 cases. There were 4 cases of secondary syphilis and 4 cases of latent syphilis. Patients with syphilis and HIV infections accounted for 44% of all the HIV-infected individuals during the year.

OBJECTIVETo explore the effects of sacral canal injection on nerve root local inflammatory factors in rat model with lumbar disc herniation, in order to identify its mechanism of treatment.

METHODSForty-eight male SD rats were randomly divided into sham operation group(group A), model group (group B), Chinese medicine group(group C) and western medicine group(group D). There were 12 rats in each group. The model of lumbar disc herniation was established using compression and inflammatory stimulation in group B, C, D. All rats were given epidural catheterization and group A and B with physiological saline (1 ml/kg), group C with mixed liquor of 2% lidocaine and compound Danshen injections and physiological saline (2:2: 16) and group D with mixed liquor of 2% lidocaine and triamcinolone acetonide injection and physiological saline (2:2:16), once a week for a total of three treatments. Four rats were killed every 1 week after injection for once, and the inflammatory factors of tumor necrosis factor (TNF-alpha), prostaglandin E2 (PGE2), interleukin-l (IL-1) and interleukin-6 (IL-6) were detected by ELISA method.

RESULTSThe levels of TNF-alpha, PGE2, IL-1 and IL-6 in compressed nerve tissues in group B were increased than those of group A (P < 0.01). The levels of PGE2, IL-1 and IL-6 in group C and D were decreased than those of group B, and group D was much less(P<0.05). There was no significant difference in level of TNF-alpha among group B, C, D (P > 0.05).

CONCLUSIONCompound compression with inflammatory stimulation can lead to massive release of inflammatory mediators, such as TNF-alpha, PGE2, IL-1 and IL-6. Both injection with compound Danshen injections and triamcinolone acetonide injections by sacral canal can reduce the levels of part inflammatory mediators (PGE2, IL-1 and IL-6), and the effect of Glucocorticoid is better than Danshen (P < 0.05).

Animals ; Dinoprostone ; analysis ; Disease Models, Animal ; Injections ; Interleukin-1 ; analysis ; Interleukin-6 ; analysis ; Intervertebral Disc Displacement ; drug therapy ; immunology ; Lumbar Vertebrae ; Male ; Rats ; Rats, Sprague-Dawley ; Salvia miltiorrhiza ; Spinal Nerve Roots ; immunology ; Triamcinolone Acetonide ; administration & dosage ; Tumor Necrosis Factor-alpha ; analysis

Objective To investigate the expression of NF-κB/p65 in nasal NK/T cell lymphomas. Methods Immunohistochemis-try and TUNEL were used to study the expression of NF-KB/p65 and cell apoptosis in 23 nasal NK/T cell lymphoma samples and 14 benign lymph node lesions. Results The NF-KB/p65 positive rates were 43.5% (10/23). The expression of NF-κB/p65 was negative correlated with apoptotic index among 23 nasal NK/T cell lymphomas(P <0. 05). The mean survival period in patients expressed NF-KB/p65 was sig-nificantly shorter than that in negative group(P <0. 05). Conclusions Apoptosis inhibited by overexpression of NF-κB/p65 might be in-volved in the development of nasal NK/T cell lymphoma. NF-κB/p65 expression may be an unfavorable prognostic factor of nasal NK/T cell lymphoma.

Objective: To clarify the contribution of inhibitory effect of the chemical composition of fingerprint characteristic peaks from different parts of Lepdium meyenii (Maca) on PDE5, and to elucidate the material basis. Methods: The HPLC fingerprints of different parts from L. meyenii were established. The isotope labelling method was adopted to test the inhibitory rate of different extracted parts on PDE5.The gray relative analysis and partial least-squares method were used to make correspond analysis of the spectrum-effect relationship. Results: The fingerprints of different extracts were established and five peaks of the total 21 characteristic common peaks were identified by HPLC spectrum of standards and LCMS-IT-TOF. The strong relevance elements including macamide alkaloids represented by peaks 22-24 were verified as the potential PDE5 inhibitors. Conclusion: A sensitive and convenient screening system for the PDE5 inhibitors via liquid scintillation counting is established. Macamides as liposoluble alkaloids could be material basis components to inhibit the activity of PDE5.This paper provides certain theory basis for resource utilization and the quality control of L. meyenii.

AIM: To investigate effects of genistein, an inhibitor of protein tyrosine kinase and vanadate, the inhibitor of protein tyrosine phophorylation, on glutamate-induced Ca2+ entry in rat primary cultured corticocerebral neurons. METHODS: The effects of drug on cytoplasmic Ca2+ level ([Ca2+]i) were invesgated with Fura-2/AM fluorescence technique. RESULTS The increase in [Ca2+]i induced by glutamate was significantly reduced by genistein (from 1 to 30 μmol·L-1) and NMDA receptor antagonist MK-801 (1 μmol·L-1), not by nimodipine (1 μmol·L-1) and SK&F96365. Vanadate (from 1 to 30 μmol·L-1) significantly enhanced glutamine-induced [Ca2+]i response. CONCLUSION: Nimdipine-sensitive Ca2+ channel and SK&F96365-sensitive Ca2+ channels are not related to glutamate-induced Ca2+ entry. It is related to the activation of protein tyrosine kinase in rat primary cultured corticocerebral neurons.

Objective To determine the effect of knocking down zebrafish faf1 gene by CRISPR/Cas9 editing technique.Methods gRNA was designed and prepared for the faf1 gene of zebrafish,and gRNA was mixed with Cas9 mRNA by microinjection into zebrafish single cell embryos.The mutant F0 generation zebrafish was screened out by enzyme digestion and gene sequencing.The mutant F0 was genetically outcrossed with the wild-type zebrafish to get the F1 heterozygous zebrafish,and the genotype of zebrafish was detected by microscopic observation.Results The faf1 gRNA and Cas9 mRNA were successfully prepared.The gRNA (gRNA6) located in the exon 6 of faf1 could shift the faf1 gene into frameshift mutations.The mutation type MU1 was screened out and the somatic cytochrome deposition delay was observed in this heterozygous zebrafish.At 4 d post fertilization (dpf),there were sarcomeric dysplasia and head shrinkage,increased hyoid angle and other craniofacial cartilage deformities.And the zebrafish died at 8 ～9 dpf.Conclusion CRISPR/Cas9 knocking out thefaf1 gene produces a new phenotype for zebrafish,with delayed pigment deposition and nodule-like change in tail muscle section.

Hormone replacement therapy (HRT), involving giving sex steroid hormones such as estrogen alone or with a progestogen, is widely used in postmenopausal women.HRT helps to relieve menopausal symptoms and has also been shown to prevent osteoporosis.Although most observational studies have showed that HRT can reduce the risks of cardio-cerebrovascular diseases, the subsequent randomized controlled trials were inconsistent with the results.This article reviews the relationship between HRT and stroke from drug type, route of administration, estrogen dosage, and initiation time.