1.Flavonoids from Selaginella uncinata.
Mei-ling YI ; Xi-feng SHENG ; Kang-ping XU ; Gui-shan TAN ; Hui ZOU
China Journal of Chinese Materia Medica 2015;40(15):3005-3008
In the current study, nine flavonoids were isolated and purified from 75% ethanol extract of Selaginella uncinata (Desv.) Spring by column chromatographic techniques over macroporous resin, polyamide, silica gel, Sephadex LH-20 and pre-HPLC. On the basis of their physico-chemical properties and spectroscopic data analyses, these compounds were elucidated as cirsimarin (1), nepitrin (2), apigenin-6-C-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside (3), apigenin-6-C-β-D-glucopyranosyl-8-C-α-L-arabinopyranoside (4), apigenin-7-O-β-D-glucopyranoside (5), 2,3-dihydroamentoflavone (6), 4'-O-methylamentoflavone (7), 2,3-dihydro-4'-O-methyl-amentoflavone (8), and 2,3,2",3"-tetrahydron-4'-O-methyl-robustaflavone (9). Compounds 1-5 belong to flavonoid glycosides and were isolated from the genus Selaginella for the first time.
Flavonoids
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analysis
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Selaginellaceae
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chemistry
2.Cloning and expression analysis in resurrection process of trehalose-6-phosphate synthase gene from Selaginella tamariscina.
Cai-Cai XI ; Wei GU ; Hong-Mei SUN ; Rong TIAN ; Qi LIU ; Xiao-Hao WANG
China Journal of Chinese Materia Medica 2018;43(24):4842-4849
Selaginella tamariscina is a typical resuscitation medicinal plant with extreme drought tolerance. Trehalose plays an important role in the resurrection process, and the trehalose-6-phosphate synthase(TPS) is the key enzyme to synthesize trehalose in plants. In this study, the sequence of TPS was obtained by splicing from the transcriptome data of S. tamariscina. After the synthesis of cDNA based on the template of total RNA, the sequence was cloned by RT-PCR for verification and then analyzed by bioinformatics methods. The results indicated that the full-length coding sequence of StTPS was 2 799 bp (GenBank accession no. MH155231), and the encoded protein contained 932 amino acids. StTPS could be located in the chloroplastid according to subcellular localization prediction. There were two conserved domains belonging to glycogen phosphorylase glycosyltransferase (GPGTF) family but no signal peptide or transmembrane domain in StTPS. The expression of StTPS was determined by qRT-PCR and the variation of trehalose content was measured by HPLC-ELSD during the resurrection process of S. tamariscina. Meanwhile, the correlation between them was analyzed. The results showed that both the expression level of StTPS and the trehalose content increased associated with the extension of dehydration time, and declined associated with the extension of rehydration time which proved a significant positive correlation between the StTPS expression level and the trehalose content. The results suggested that the StTPS probably plays a central role in recovery process in S. tamariscina.
Amino Acid Sequence
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DNA, Complementary
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Glucosyltransferases
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Selaginellaceae
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Trehalose
3.Paleo-polyploidization in Lycophytes.
Jinpeng WANG ; Jigao YU ; Pengchuan SUN ; Chao LI ; Xiaoming SONG ; Tianyu LEI ; Yuxian LI ; Jiaqing YUAN ; Sangrong SUN ; Hongling DING ; Xueqian DUAN ; Shaoqi SHEN ; Yanshuang SHEN ; Jing LI ; Fanbo MENG ; Yangqin XIE ; Jianyu WANG ; Yue HOU ; Jin ZHANG ; Xianchun ZHANG ; Xiu-Qing LI ; Andrew H PATERSON ; Xiyin WANG
Genomics, Proteomics & Bioinformatics 2020;18(3):333-340
Lycophytes and seed plants constitute the typical vascular plants. Lycophytes have been thought to have no paleo-polyploidization although the event is known to be critical for the fast expansion of seed plants. Here, genomic analyses including the homologous gene dot plot analysis detected multiple paleo-polyploidization events, with one occurring approximately 13-15 million years ago (MYA) and another about 125-142 MYA, during the evolution of the genome of Selaginella moellendorffii, a model lycophyte. In addition, comparative analysis of reconstructed ancestral genomes of lycophytes and angiosperms suggested that lycophytes were affected by more paleo-polyploidization events than seed plants. Results from the present genomic analyses indicate that paleo-polyploidization has contributed to the successful establishment of both lineages-lycophytes and seed plants-of vascular plants.
Evolution, Molecular
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Genome, Plant
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Genomics
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Phylogeny
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Polyploidy
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Selaginellaceae/genetics*
4.A new selaginellin derivative from Selaginella pulvinata.
Yuan CAO ; Yong-Ping WU ; Jin-Ao DUAN
Acta Pharmaceutica Sinica 2015;50(2):199-202
Abstract: A new selaginellin derivative named as selaginellin S (1) was isolated from the whole plants of Selaginella pulvinata (Hook. et Grev.) Maxim. (Selaginellaceae), together with a known one (selaginellin M, 2). Compounds 1 and 2 were separated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were determined on the basis of extensive spectroscopic analysis including IR, MS, 1D and 2D NMR experiments, as well as ECD calculations. Compound 1 is a key intermidiant in the biosynthesis pathway of selaginellins. Compound 2 is first reported in this plant.
Biphenyl Compounds
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chemistry
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isolation & purification
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Cyclohexanones
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chemistry
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isolation & purification
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Molecular Structure
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Selaginellaceae
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chemistry
5.A new cyclopeptide from Selaginella tamariscina.
Xin-Jia YAN ; Jing WEN ; Yang SONG ; Dong-Mei SHA ; Ma-Li-Niu SHA ; Shao-Shan ZHANG ; Yuan LIU
China Journal of Chinese Materia Medica 2022;47(16):4391-4394
One new cyclopeptide was isolated from the ethyl acetate fraction of the 75% EtOH extract of Selaginella tamariscina by various column chromatography methods(HP-20, polyamide and semi-preparative HPLC). Its structure was identified as selapeptin A(1) by extensive spectroscopic analysis(HR-ESI-MS, 1 D and 2 D NMR). Compound 1 was evaluated for cytotoxic activities by MTT assay. It showed potent cytotoxic activity against B16 F10 with the inhibition rate of 51.57%±4.34% at 40 μmol·L~(-1) while had no impacts on MDA-MB-231 and MDA-MB-468 at 100 μmol·L~(-1).
Chromatography, High Pressure Liquid
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Peptides, Cyclic/pharmacology*
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Selaginellaceae/chemistry*
6.Molecular characteristics of two Phi glutathione S-transferases in Selaginella moellendorffii.
Yuanjie ZHANG ; Zhiling YANG ; Hailing YANG
Chinese Journal of Biotechnology 2016;32(7):927-936
Glutathione S-transferase (GST) is important in plants to resist various stresses. In this study, two Phi GST genes (SmGSTF1 and SmGSTF2) were cloned from Selaginella moellendorffii. SmGSTF1 and SmGSTF2 genes encode proteins of 215 amino acid residues. Gene expression analysis showed that the two genes were expressed in roots, stems and leaves. The recombinant SmGSTF1 and SmGSTF2 proteins were overexpressed in Escherichia coli, and purified by Ni-affinity chromatography. SmGSTF1 and SmGSTF2 had the catalytic activity towards 1-Chloro-2,4-Dieitrobenzene, 4-Chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl), and 4-Nitrobenzyl chloride substrates. SmGSTF1 also had the activity towards Fluorodifen and Cumyl hydroperoxide (Cum-OOH), whereas SmGSTF2 not. The enzyme kinetics analysis showed that SmGSTF1 and SmGSTF2 had high affinity towards glutathione, and low affinity towards 1-Chloro-2, 4-Dieitrobenzene. The enzymatic activity of SmGSTF1 and SmGSTF2 had high catalytic activity between pH 7 and 8.5, and between 45 and 55 °C. SmGSTF1 and SmGSTF2 may have an important role in the resistance of Selaginella moellendorfii against stress.
Amino Acid Sequence
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Cloning, Molecular
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Escherichia coli
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Glutathione Transferase
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genetics
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metabolism
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Plant Proteins
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genetics
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metabolism
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Selaginellaceae
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enzymology
7.Simultaneous determination of selaginellins and biflavones in Selaginella tamariscina and S. pulvinata by HPLC.
Yuan CAO ; Yongping WU ; Xiaowen ZHOU ; Fang QIAN ; Huijia FAN ; Qiang WANG
China Journal of Chinese Materia Medica 2012;37(9):1254-1258
OBJECTIVETo establish a HPLC-DAD model for the simultaneous determination of two selaginellins (selaginellin and selaginellin B) and four biflavones (amentoflavone, sequoiaflavone, hinokiflavone and isocryptomerin) contained in 10 batches of Selaginella tamariscina and 12 batches of S. pulvinata produced in different areas.
METHODThe analysis was performed on a Waters Cosmosil C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitril-0.1% formic acid as mobile phase in a linear gradient mode. The detection wavelength was set at 280, 337 nm. The flow rate was kept at 1.0 mL x min(-1), and the column temperature was 30 degrees C.
RESULTThe six active constituents showed significant different in content. Amentoflavone in S. tamariscina contains (5. 628-9. 184 mg x g(-1)) is more than that contained in S. pulvinata (0.823-7.131 mg x g(-1)), while selaginellin in S. pulvinata (0.123-0.593 mg x g(-1)) is more than that contained in S. tamariscina (0.067-0.133 mg x g(-1)). All the calibration curves showed good linear correlation coefficients (r > 0.9997) over the wide test ranges.
CONCLUSIONThe developed HPLC-DAD method is simple, sensitive and accurate and has the good repeatability in separation, which is available for the quality control of S. tamariscina and S. pulvinata.
Biflavonoids ; chemistry ; Biphenyl Compounds ; chemistry ; Chromatography, High Pressure Liquid ; methods ; Cyclohexanones ; chemistry ; Flavones ; chemistry ; Flavonoids ; chemistry ; Selaginellaceae ; chemistry
8.Study on HPLC fingerprint characteristics of Selaginella plants.
Xiao-Lei FAN ; Ding-Rong WAN ; Cong-Jin YE ; Ke-Li CHEN
China Journal of Chinese Materia Medica 2007;32(20):2102-2106
OBJECTIVETo establish a method to analyze HPLC fingerprint characteristics of 10 plants from Selaginella.
METHODHPLC was applied for establishment of fingerprints, which were used to evaluate and distinguish the different species of Selaginella.
RESULTThe different species from Selaginella showed different HPLC fingerprint characteristic. The samples of the same species but collected in different period, different environment or different locations showed certain difference in fingerprints
CONCLUSION2 important mutual fingerprint peaks were found in the 10 plants of Selaginella species and 5 peaks can be used as "main fingerprint peaks". The dates of these peaks can used for assessment of phylogenetic relation among species and evaluation of quality.
Biflavonoids ; analysis ; China ; Chromatography, High Pressure Liquid ; methods ; Plants, Medicinal ; chemistry ; classification ; Selaginellaceae ; chemistry ; classification ; Species Specificity
9.Investigations and studies on medicinal plants from Selaginellaceae in Hubei Province.
Ding-Rong WAN ; Ke-Li CHEN ; Ya-Hua ZHAN
China Journal of Chinese Materia Medica 2005;30(19):1507-1510
OBJECTIVETo investigate the species, the distribution and the utilization of the medicinal plants from Selaginellaceae in Hubei Province.
METHODThrough field investigations and comparing the collected specimens and literatures, the classification and identification of the species in Hubei Province were studied.
RESULTThe results indicated that 15 species of plants from Selaginellaceae in Hubei, including 14 medicinal and 1 newly recorded species existed. The distribution and use in folk medicine were investigated. And the morphological description of several species was appended.
CONCLUSIONThe results provided a basis for the exploitation and utilization of the medicinal plant resources of Selagingellaceae.
China ; Conservation of Natural Resources ; Drugs, Chinese Herbal ; isolation & purification ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy ; Plants, Medicinal ; anatomy & histology ; chemistry ; classification ; Selaginellaceae ; anatomy & histology ; chemistry ; classification
10.Study on the chemical constituents of Selaginella tamariscina (Beauv.) Spring.
Xiao-ke ZHENG ; Yue-feng BI ; Wei-sheng FENG ; She-po SHI ; Ji-feng WANG ; Jian-zhao NIU
Acta Pharmaceutica Sinica 2004;39(4):266-268
AIMTo study the chemical constituents of Selaginella tamariscina (Beauv.) Spring.
METHODSVarious chromatographic techniques were used to separate and purify the chemical constituents. Their physico-chemical properties and spectral data were used to elucidate the structures.
RESULTSFour compounds were isolated from the n-BuOH fraction of the water-extracts. Their structures were identified as 1-hydroxy-2-[2-hydroxy-3-methoxy-5-(1-hydroxyethyl)-phenyl]-3-(4-hydroxy-3,5-dimethoxy)-propane-1-O-beta-D-glucopyranoside (tamariscinoside B, I), adenosine (II), guanosine (III), arbutin (IV).
CONCLUSIONTamariscinoside B (I) is a new compound, while the others were isolated from Selaginella for the first time.
Adenosine ; chemistry ; isolation & purification ; Arbutin ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Guanosine ; chemistry ; isolation & purification ; Molecular Conformation ; Molecular Structure ; Plants, Medicinal ; chemistry ; Selaginellaceae ; chemistry