No abstract available.
Female ; Metrorrhagia*

No abstract available.
Animals ; Fetus* ; Mice* ; Paraquat*

Tbe presence of certain HPV types in the female genital tract was associated with a number of diseases, including condyloma, cervical, vaginal and vulvar intraepithelial neoplasia and carcinoma. It is generally accepted that these viruses are predominantly sexually transmitted. HPV cannot be cultured in vitro, and immunological tests are inadequate. Indirect evidence of anogenital HPV infection can be obtained through the physical examination and by the presence of characteristic cellular changes associated with viral replication in Pap smear or biopsy specimens. Alternately cervical scrapes can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA, Historically, HPV 16 and HPV 18 have been regarded as high risk cancer associated HPVs and HPV types 6, 11, 42, 43 and 44 as low risk HPVs. Subsequently HPV types 31, 33, 34, 35, 45, 51, 52, 56 and 58 have been demonstrated to have and intemediate association with cancer, This study was performed for high and intermediate types HPV DNA detection on cervical carcinoma with Virapap Probe B. HPV DNA were detected 82.3% on cervical carcinoma and 7,1% on control samples. HPV DNA test will be apply as a triage for cervical cancer detecting test.
Biopsy ; DNA ; Female ; Human papillomavirus 16 ; Human papillomavirus 18 ; Human Papillomavirus DNA Tests ; Humans* ; Immunologic Tests ; Nucleic Acid Hybridization ; Papilloma* ; Physical Examination ; Triage ; Uterine Cervical Neoplasms*

No abstract available.
Cervix Uteri* ; Female ; Neuroendocrine Cells*

OBJECTIVE: The purpose of this study is to determine the effects of glutathione on cisplatin-induced cytotoxicity of human cervical carcinoma cell lines (SiHa: squamous cell carcinoma cell, CaSki: epidermoid metastatic carcinoma cell). METHODS: Human cervical carcinoma cells (SiHa, CaSki) were incubated with culture media (RPMI1640) in the presence of cisplatin and/or buthionine sulfoximine (BSO), as a inhibitor of gamma-glutamyl- cysteine synthetase, and/or glutathione (GSH) and/or 2-oxo 4-thiazolidine carboxylic acid (OTC). The viable cells were examined by using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and was determined by spectrophotometer at 570 nm. RESULTS: The incubation of cervical cells with cisplatin resulted in an decreasing cells viability by dose response. The MTT reduction rate were not different by BSO (5 mM) treatment in cervical cell lines. The viable cells were increased significantly by glutathione (5 mM) or OTC (5 mM) in cisplatin-treated cell lines. CONCLUSION: gamma-glutamylcysteine synthetase inhibitor had no effect on cisplatin toxicity. GSH and OTC had effect on cisplatin cytotoxicity. So, These result suggested that cervical cancer line cells were more susceptive to protective effects of glutathione and OTC than BSO on cisplatin induced-toxicity.
Buthionine Sulfoximine ; Carcinoma, Squamous Cell ; Cell Line* ; Cisplatin ; Culture Media ; Cysteine ; Glutathione* ; Humans* ; Ligases ; Uterine Cervical Neoplasms*

Glutathione has a key role in several detoxification reactions and in the protection against injury caused by reactive oxygens. Pregnancy-induced hypertension(PIH) is associated with endothelial cell dysfunction. Such dysfunction could be caused by oxidative stress. There is evidence of increased activity of free radicals in PIH, but little is known about the part played by changes in specific antioxidants. In this study, the changes of glutathione levels were investigated in blood of patients with PIH, and cord blood of these patients was also investigated. The glutathione levels in cord blood of neonates from hypertensive pregnant women were significantly higher than in cord blood from normotensive pregnant women. The changes of gamma-glutamuylcysteine synthetase and of glutathione s-transferase in placenta were not significant, but gamma-glutamyl transpeptidase activity increased significantly in placenta of hypertensive pregnant women. These results suggest that the increased glutathione in cord blood of patients with PIH may be due to increased glutathione interorgan transport resulting from incerased activity of the placenta gamma-glutamyl transpeptidase.
Antioxidants ; Endothelial Cells ; Female ; Fetal Blood ; Free Radicals ; gamma-Glutamyltransferase ; Glutathione Transferase ; Glutathione* ; Humans ; Hypertension, Pregnancy-Induced* ; Infant, Newborn ; Ligases ; Oxidative Stress ; Oxygen ; Placenta* ; Pregnancy ; Pregnant Women

OBJECTIVE: For many years, the Papanicolaou smear has been used to detect pre-malignant and malignant disease of the cervix. Although the use of cytology in screening has reduced morbidity and mortality from invasive cervical cancer, there has been concerned about the low sensitivity of cervical cytology in detection of preclinical disease of the cervix. So cervicography is introduced. In order to find out the efficacy of cytology and cervicography in early diagnosis of cervical cancer, this study included 1028 patients who visited the Department of Obstetrics and Gynecology, Cho-sun University Hospital From December 1, 1995 to March 31, 1998. And we choosed 321 samples who underwent biopsies. METHODS: Women simultaneously underwent an exo and endo-cervical smear, and then two cervicography was obtained with applying 5% acetic acid. Because all of the subjects choosed in statistical analysis were examined by colposcopy or postoperative biopsies, the presence or abscence of disease was determined by the histologic findings. RESULTS: 1. The sensitivity and specificity of cytology was 55.4% and 86.6%. 2. The sensitivity and specificity of cervicography was 71.3% and 59.7%. 3. When cervicography and Papanicolaou smear were used conjointly, the sensitivity and specificity was 90.1% and 58.8%. 4. However the specificity of the combined test was lower than cytology alone, the sensitivity of the combined test was significantly higher. CONCLUSION: The double combined tests of Papanicolaou smear and Cervicography were very useful in the early diagnosis of cervical neoplasia.
Acetic Acid ; Biopsy ; Cervix Uteri ; Colposcopy ; Early Diagnosis ; Female ; Gynecology ; Humans ; Mass Screening* ; Mortality ; Obstetrics ; Papanicolaou Test ; Sensitivity and Specificity ; Uterine Cervical Neoplasms*

The development of cancer in a pre-existing benign cystic teratoma of the ovary with distant metastasis to the supraclavicular lymph nodes has not been previously reported. We experienced a case of a 28-year-old woman with distant metastasis from small cell cacinoma arising in benign mature cystic teratoma of the ovary to the supraclavicular lymph nodes. The staging laparotomy and total abdominal hysterectomy with both salpingooophorectomy were peformed. There was no metastatic site except the supraclavicular lymph nodes, thereafter adjuvant chemotherapies with bleomycin, etoposide, cisplatin have been doing. We report a case of benign cystic teratoma with malignant change and metastasis with the literature.
Adult ; Bleomycin ; Carcinoma, Small Cell* ; Cisplatin ; Drug Therapy ; Etoposide ; Female ; Humans ; Hysterectomy ; Laparotomy ; Lymph Nodes* ; Neoplasm Metastasis* ; Ovary* ; Teratoma*

OBJECTIVE: Our purpose was to determine the clinical value of human papilloma virus deoxyribonucleic acid testing with the hybrid capture test, specifically to examine whether human papillomavirus testing could identify which women with Papanicolaou smears read as atypical squamous cells of undetermined significance were most likely to have histologically confirmed cervical intraepithelial neoplasia. STUDY DESIGN: Hybrid capture testing for high-risk human paillomavirus types(type 31, 33, 35, 45, 51, 52, 56), repeat Papanicolaou smears and colposcopically directed biopsies were performed concurrently on 55 women refered to chosun university hospital with a previous Papanicolaou smear read as atypical squamous cells of undetermined significance. RESULTS: Human papillomavirus eoxyribonucleic acid positivity was associated with a six fold increased likelihood of histologic confirmation of cervical intraepithelial neoplasia. The sensitivity of hybrid capture for any cervical intraepithelial neoplasia was 92% (12/13) and specificity was 86%(36/42), whereas the corresponding values for the repeat Papanicilaou smear was 85%(11/13) and 38%(16/42), respectively. CONCLUSIONS: Testing for human papillomavirus deoxyribonucleic acid with hybrid capture appears to offer an effective means by which patients whose cervical Papanicolaou smears have been read as atypical squamous cells of undetermined significance could be triaged for colposcopy. In particular, high sensitivity and specificity for cervical intraepithelial neoplasia could be maintained.
Biopsy ; Cervical Intraepithelial Neoplasia ; Colposcopy ; DNA ; Female ; Humans* ; Papanicolaou Test ; Papilloma* ; Sensitivity and Specificity

No abstract available.
Pregnancy*