2.Viability of preserved Cryptosporidium baileyi oocysts.
Chan Gu SURL ; Se Min KIM ; Hyeon Cheol KIM
The Korean Journal of Parasitology 2003;41(4):197-201
The present study was undertaken to determine the viability and infectivity of oocysts of Cryptosporidium baileyi that had been stored from 1 to 40 months at 4 degrees C preserved in 2.5% potassium dichromate solution. Oocysts of C. baileyi were purified from the feces of experimentally infected chickens using discontinuous sucrose gradients. Subsequently, the purified oocysts were suspended in 2.5% potassium dichromate solution at a concentration of 1 x 10 (7) organism/ml, and their viabilities were assessed by nucleic acid staining, histologic examination, and infectivity to 2-day-old chickens. All chickens inoculated with oocysts that had been stored for 1-18 months developed patent infections, while chickens infected with older oocysts remained uninfected. Between 5.8% and 82.2% of the oocysts, stored at 4 degrees C in 2.5% potassium dichromate solution, were found to be viable, as determined by nucleic acid staining. Parasite colonization in the bursa of Fabricius was detected in the microvillus border of bursal epithelium. The finding that C. baileyi oocysts remain infective to chickens for at least 18 months offers important time-saving advantages to investigators who frequently require large numbers of oocysts.
Animals
;
Bursa of Fabricius/parasitology
;
Chickens/*parasitology
;
Coloring Agents
;
Cryptosporidiosis/parasitology/pathology/*veterinary
;
Cryptosporidium/drug effects/*growth & development/pathogenicity
;
Feces/parasitology
;
Oocysts/drug effects/*growth & development/pathogenicity
;
*Organic Chemicals
;
*Potassium Dichromate/pharmacology
;
Poultry Diseases/parasitology/pathology
;
Preservation, Biological/*methods
;
Staining and Labeling
3.Prognostic Value of Somatosensory Evoked Potentials in Comatose Patients after Cardiopulmonary Resuscitation.
Se Min CHOI ; Dong Rul OH ; Seung Pil CHOI ; Kyu Nam PARK ; Se Kyung KIM
Journal of the Korean Society of Emergency Medicine 2000;11(4):450-456
BACKGROUND: The improved technique for cardiopulmonary resuscitation(CPR) has resulted in the survival of many patient who experienced cardiac arrest. However, mortality in resuscitated patients is high, and the survival rate without brain damage is very low. Various neurological examination models, neuro-imaging techniques, electrophysiological procedures, and biochemical tests have been studied with respect to the detection of cerebral damage and outcome, but an early, reliable prediction of individual outcomes is still uncertain. METHODS: We studied twenty patient who had been in a coma for more than 24 hours after CPR, Somatosensory evoked potentials(SEP) were measured within the first three days after CPR. RESULTS: Of the twenty patients, seven patients(35%) had a good outcome, and thirteen patients(65%) had a bad outcome. Of the eleven patients with loss of the cortical evoked potential's N20 peak, all had a bad outcome. CONCLUSION: SEPs are of great benefit in prognostic evaluation after CPR.
Brain
;
Cardiopulmonary Resuscitation*
;
Coma*
;
Evoked Potentials, Somatosensory*
;
Heart Arrest
;
Humans
;
Mortality
;
Neurologic Examination
;
Survival Rate
4.A Study of Case-Based Adult Advanced Cardiac Life Support(ACLS) course in Korea.
Kyu Nam PARK ; Se Min CHOI ; Seung Hyun PARK ; Eun Young YOO ; Se Kyung KIM
Journal of the Korean Society of Emergency Medicine 1999;10(2):191-197
BACKGROUND: To describe the fast experience of case-leased advanced cardiac life support(ACLC) course in Korea. METHODS: We have given case-based ACLC course to 13 nurses(6 emergency nurses, 4 coronary care unit muses, 3 professors) and 17 physicians(4 emergency physicians, 12 emergency residents, 1 intem). We performed the case-based ACLS course according to 1992 American Heart Association guidelines and recommendations for advanced cardiac life support by american ACLS instructors(1 pulmonologist, 4 critical care nurses). We performed final theoretical written test and 2 times written survey (immediate and 100th day after the course) about the course. RESULTS: On final written test, all practitioners answered at leasts 70% of the questions correctly. There was no significant difference between nurses and physicians(86.2+/-3.6 of physicians and 82.5+/-6.8 of nurses, p=0.06). 90%of participants considered that case-based advanced cardiac life support was acceptable. 100th day after the course, 93%of participants answered that ACLS course have been helpful on his/her job and also want retraining of ACLS course. 70%of participants considered that ideal ACLS training committee in Korea is the Korean Society of Emergency Medicine. CONCLUSION: Case-based ACLS course is a useful educational method far physicians and nurses in Korea. In the future, we should organize Korean resuscitation committee and then make guidelines for ACLS, and then continuously educate physicians and nurses.
Adult*
;
Advanced Cardiac Life Support
;
Alprostadil
;
American Heart Association
;
Coronary Care Units
;
Critical Care
;
Emergencies
;
Emergency Medicine
;
Humans
;
Korea*
;
Resuscitation
5.Rhabdomyolysis in Doxylamine Succinate Overdose.
Mi Jin LEE ; Dong Rul OH ; Won Jae LEE ; Se Min CHOI ; Se Kyung KIM
Journal of the Korean Society of Emergency Medicine 2000;11(1):127-136
BACKGROUND: Doxylamine succinate(DS) is an antihistamine commonly used as an over-the-counter medication to relieve insomnia and frequently involved in overdoses. Its overdoses are dominated by anticholinergic effect. Recently it was revealed that DS had a direct effect on muscle, while its exact mechanism is not clear yet. We evaluated the patients with rhabdomyolysis induced by DS overdose for patients disposition based upon clinical decision, especially by creatinine phosphokinase(CPK). METHODS: We reviewed retrospectively the medical records of patients admitted by DS overdose from Jan. 1998 to Oct. 1999. Seventy and nine cases of DS overdose were evaluated with respect to age and sex distribution, amount ingested, clinical symptomatology, time from ingestion to visit, pattern of CPK, amount of bicarbonate used as therapy, complication and prognosis, especially in patients complicated rhabdomyolysis. RESULTS: Rhabdomyolysis, diagnosed as more than 1,000I. U/L of CPK, has been noted in 25(31.6%) of 79 cases of DS overdose visited to our emergency department(ED). In patients diagnosed rhabdomyolysis, the number of man was 10 cases(40%) and the number aged between 20 and 40 years was 22 cases(88%). The average time from DS ingestion to ED visit was 459 minutes. The amount of DS ingested was 500-5,000mg(mean, 1,980mg). 13(52%) cases ingested less than 2,250mg of DS. The initial levels of CPK(range, 48-14900I. U/L; normal range, 26-200I. U/L) after admitting to our emergency department were normal in 15 cases(60%) of rhabdomyolysis patients. The range of peak CPK levels after ingestion was 607 to 412,500I. U/L(mean, 33,550I. U/L). Its peak time was 6 to 96 hours(mean, 28.96 hours). In 14 cases(67%) of 21 visiting within 24 hours after ingestion, peak time of CPK ranged 12 to 24 hours after ingestion. The amount of bicarbonate used as therapy of rhabdomyolysis ranged 100 to 2,740mEq(mean, 656mEq) and all patients was discharged after improvement without other complication including acute renal failure. CONCLUSIONS : Although patients ingested less than 2,250mg of DS, emergency physicians should observe them more than 24 hours after DS ingestion with CPK follow-up after gastric irrigation and charcoal administration.
Acute Kidney Injury
;
Charcoal
;
Creatinine
;
Doxylamine*
;
Eating
;
Emergencies
;
Emergency Service, Hospital
;
Follow-Up Studies
;
Gastric Lavage
;
Humans
;
Medical Records
;
Prognosis
;
Reference Values
;
Retrospective Studies
;
Rhabdomyolysis*
;
Sex Distribution
;
Sleep Initiation and Maintenance Disorders
;
Succinic Acid*
6.Treatment of Pathologic Dislocation of the Hip after Septic Arthritis in Infancy
Young Min KIM ; Choon Ki LEE ; Se Hyun CHO
The Journal of the Korean Orthopaedic Association 1983;18(6):1113-1121
No abstract available in English.
Arthritis, Infectious
;
Dislocations
;
Hip
7.Upper limb salvage using free forearm flap harvested from the non-replantable amputation part: cases report.
Woo Young JANG ; Rong Min BAEK ; Soo Shin KIM ; Se Min BAEK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1991;18(6):1148-1152
No abstract available.
Amputation*
;
Forearm*
;
Upper Extremity*
9.CLINICAL EXPERIENCE OF OBLIQUE FACIAL CLEFTS (REPORT OF 5 CASES).
Jung Wook HAHM ; Jino KIM ; Rong Min BAEK ; Kap Sung OH ; Se Min BAEK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1997;24(5):976-986
No abstract available.
10.Clinical experience of ear elevation after reconstruction of microtia.
Kyung Ha HWANG ; Jin O KIM ; Rong Min BEAK ; Kap Sung OH ; Se Min BAEK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1998;25(5):807-817
The basic technique for total reconstruction of the microtia was established by Tanzer, who utilized autologous rib cartilages for constructing the auricular framework. In order to decrease the number of surgical stages and to achieve maximal convolution, we employed a surgical procedure with simultaneous three layered costal cartilage grafting for the high profiled auricle, concha formation and lobule rotation. However, we performed ear elevation as an additional procedure for some patients who had decreased helical height due to absorption of cartilage framework or postoperative trauma and who had wanted to wear the spectacles or to have more natural appearance of auriculocephalic sulcus. From August 1988 to October 1997 we had performed surgeries for the ear elevation of 58 patients in 177 patients with total ear reconstruction, using various elevation methods; skin graft, local flap, and local with costal cartilage block. When the ear elevation was performed with skin graft, postoperative contraction of the grafted skin was inevitable. In cases with two skin flaps, it was difficult to stabilize and maintain the correct projection of the constructed ear. So we elevated the reconstructed ear by utilizing a costal cartilage block, two skin flaps to cover the posterior region and skin graft. We conclude that the local flap with costal cartilage block is one of the most favorable methods in ear elevation which can maintain the adequate projection and make natural looking auriculocephalic sulcus.
Absorption
;
Cartilage
;
Ear*
;
Eyeglasses
;
Humans
;
Ribs
;
Skin
;
Transplants