Dental Offices ; Dentistry ; Mouth ; Pathology

Narcotics and Some Neuromuscular blockers are used for open heart sugery anesthesia. Rapid induction of intravenous narcotics techniques may cause some reductjon in blood pressure and systemic vascular resistance, however, the selection of a narcotic and neuromuscular blocker influences subsequent Hemodynamic responses. 72 patients having open heart surgery were investigated using three combination of a narcotic and neuromuscular blocker: Group I (fentanyl 50 ug/kg, pancuronium 100 ug/kg): Group II (fentanyl 50 ug/kg, Vecuronium 80 ug/kg) Group III (sufentanil 10 u/kg, vecuronium 80 u/kg), each combination being administered over 2 minutes. Hemodynamic functions were then monitored for 10 minutes before tracheal intubation. 1) Significant changes included increases in heart nate in the groups receiving pancuronium and decrease in those receiving vecuronium. 2) In all groups mean arterial pressure initially decreased: systemic vascular resistance index decreased significantly in all groups except sufentanil and vecuronium group. 3) Cardiac index decreased significantly only in ufentanil-Vecuronium groups. Time of onset of neuromuscular blockade did not differ among the three groups, but transient chest wall rigidity occurred significantly more often with sufentanil than with fentanyl. Overall, the fentanyl-Pancuronium combination afforded the greatest hemodynamic stability, whereas the sufentanil-Vecuronium Combination proved least Satisfactory because of bradycardia, hypotension, increase of chest wall rigidity.
Anesthesia* ; Arterial Pressure ; Blood Pressure ; Bradycardia ; Cardiovascular System* ; Fentanyl ; Heart* ; Hemodynamics ; Humans ; Hypotension ; Intubation ; Narcotics ; Neuromuscular Blockade ; Neuromuscular Blocking Agents ; Pancuronium ; Sufentanil* ; Thoracic Surgery ; Thoracic Wall ; Vascular Resistance ; Vecuronium Bromide*

Hemorrhage ; Mouth Mucosa

In order to provide a basis for studying the molecular mechanism of pharmacological action of local anesthetics and to develop a fluorescence spectroscopic method which can detect the microviscosity of native and model membranes using intramolecular excimerization of 1,3-di(l-pyrenyl)propane (Py-3-Py), we examined the effect of lidocaine cntdot HCl on the microviscosity of model membranes of phosphatidylcholine fraction extracted from synaptosomal plasma membrane vesicles (SPMVPC). The excimer to monomer fluorescence intensity ratio (I'/I) of Py-3-Py in liquid paraffin was a simple linear function of T/eta. Based on this calibration curve, the microviscosity values of the direct probe environment in SPMVPC model membranes ranged from 234.97 +/- 48.85 cP at 4degreeC to 19.21 +/- 1.11 cP at 45degreeC. At 37degreeC, a value of 27.25 +/- 0.44 cP was obtained. The lidocaine cntdot HCl decreased the microviscosity of SPMVPC model membranes in a concentration-dependent manner, with a significant decrease in microviscosity value by injecting the local anesthetic even at the concentration of 0.5 mM. These results indicate that the direct environment by Py-3-Py in the SPMVPC model membranes is significantly fluidized by the lidocaine cntdot HCl. Also, the present study explicitly shows that an interaction between local anesthetics and membrane lipids is of importance in the molecular mechanism of pharmacological action of lidocaine cntdot HCl.
Anesthetics, Local ; Calibration ; Cell Membrane ; Fluorescence ; Lidocaine* ; Membrane Lipids ; Membranes* ; Mineral Oil ; Phosphatidylcholines*

Fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) was used to evaluate the effects of dopamine cntdot HCl on the range of the rotatioanl mobility of bulk bilayer structure of the synaptosomal plasma membrane vesicles (SPMV) isolated from whole bovine brain. In a dose-dependent manner, dopamine decreased the anisotropy (gamma), limiting anisotropy (gammainfin) and order parameter (S) of DPH in the membranes. These indicate that dopamine increased the rotational mobility of the probe in the neuronal membranes. Cationic 1-(4-(trimethylammonio)-phenyl)-6-phenylhexa-1,3,5-hexatriene (TMA-DPH) and anionic 3-(p-(6-phenyl)-1,3,5-hexatrienyl)-phenylpropionic acid (PRO-DPH) were utilized to examine the range of transbilayer asymmetric rotational mobility of the neuronal membranes. Dopamine had a greater increasing effect on the mobility of the inner monolayer as compared to the outer monolayer of the neuronal membranes. It has been proven that dopamine exhibits a selective rather than nonselective fluidizing effect within the transbilayer domains of the SPMV.
Anisotropy ; Brain* ; Cell Membrane* ; Diphenylhexatriene ; Dopamine* ; Fluorescence Polarization ; Membranes ; Neurons ; Plasma*

No abstract available.
Female ; Humans ; Melanins*