1.Expression of Topoisomerase II alpha and Ki-67 in Invasive Mammary Carcinoma and Their Clinicopathologic Implication.
Sang Min OH ; Dae Cheol KIM ; Se Heon CHO
Journal of the Korean Surgical Society 2007;73(3):204-209
PURPOSE: Various prognostic indicators have been identified for mammary carcinomas, but the issue of their significance remains unsettled. The prognostic impact of c-erb B2, Ki-67 and topoisomerase II alpha expression was investigated in relation to prognostic factors for carcinomas of the breast and to the tumor cell growth fraction. METHODS: One hundred eighteen cases of invasive mammary carcinoma were investigated by immunohistochemical staining for c-erb B2, topoisomerase II alpha, and Ki-67. Clinicopathologic parameters were compared with the expression pattern and incidence of c-erb B2, topoisomerase II alpha and Ki- 67 in invasive mammary carcinoma. RESULTS: C-erb B2 showed significant correlation with topoisomerase II alpha (P<0.05), but others were not significant. Topoisomerase II alpha and Ki-67 index closely paralleled each other, indicating that both reflect the proliferate activity of tumor cells and were associated with high nuclear and histological grade, ER and PR expression (P<0.05). CONCLUSION: These results indicate that ki-67 and topoisomerase II alpha proteins might play a role in tumor progression of breast carcinoma. The Ki-67 and topoisomerase II alpha index may be proliferate factors of breast cancer. In addition, the increase expression of Ki-67 and topoisomerase II alpha and hormone receptor were closely correlated each other, and could be used as factors suggesting poor prognosis in breast carcinoma.
Breast
;
Breast Neoplasms
;
DNA Topoisomerases, Type II*
;
Incidence
;
Prognosis
2.A Case of Rectal Cancer in a Patient with Neurofibromatosis Type 1.
Se Heon OH ; Jai Hyuen LEE ; Hwan NAMGUNG
Journal of the Korean Society of Coloproctology 2012;28(3):170-173
A rectal cancer was found in a 67-year-old man with a history of neurofibromatosis type 1. A low anterior resection was performed, and he received concurrent chemoradiation for 6 months. Twelve months after the surgery, a tumor was found at the anastomotic site by positron emission tomography-computed tomography and colonoscopy and was mistaken as anastomotic site recurrence. The tumor was confirmed as an inflammatory myofibroblastic tumor through transanal excision.
Aged
;
Colonoscopy
;
Colorectal Neoplasms
;
Electrons
;
Humans
;
Myofibroblasts
;
Neurofibromatoses
;
Neurofibromatosis 1
;
Rectal Neoplasms
;
Recurrence
3.A Case of Insulinoma which was treated by Laparoscopic Enucleation.
Ie Byung PARK ; Young Jae OH ; Jung Heon OH ; Nan Hee KIM ; Sang Jin KIM ; Se Hyun BAEK ; Seob Sub CHOI ; Sung Ok SEO ; Min Kyung KIM
Journal of Korean Society of Endocrinology 1998;13(4):665-669
Insulinoma is a functional endocrine tumor arising from the beta cells of islets of Langerhans of pancreas. The only effective treatment of insulinoma was surgical removal of the tumor. Recently, laparoscopic surgery for islet cell tumors of the pancreas is introduced. Laparoscopic enucleation or resection of benign islet tumors results in a shorter hospital recovery and is a good alternative to open surgery We report a case of insulinoma that was treated sucessfully by laparoscopic enucleation.
Adenoma, Islet Cell
;
Insulinoma*
;
Islets of Langerhans
;
Laparoscopy
;
Pancreas
4.A Case of Parathyroid Cancer with a Local Metastatic Focus Revealed by 99mTc-sestamibi scan.
Soo Mi KIM ; Shin Gon KIM ; Ie Byung PARK ; Dong Hyun SHIN ; Jung Heon OH ; Nan Hee KIM ; Se Hyun BAEK ; Seob Sub CHOI ; Jung Hwan LEE
Journal of Korean Society of Endocrinology 1997;12(4):627-632
Carcinoma of the parathyroid gland is rare, comprising only 0.1% to 5% of all patients with primary hyperparathyroidism. It presents with severe hypercalcemia, bone disease, palpable neck mass, renal involvement and etc. Since the initial operation offers the best chance for cure, preoperative localization and intraoperative recognition of parathyroid cancer are essential. Recently parathyroid imaging has been described with 99mTc-sestamibi as an alternative to 201Tl. This newer agent has many physical and dosirnetric advantages and represents higher detection sensitivity than 201Tl-99mTc subtraction scan. We experienced a 41-year-old man presenting with recurrent hyperparathyroidism in spite of 2 previous operations. In preoperative localization, there was no abnormal uptake in 201Tl-99mTc subtraction scan but 99mTc-sestamibi scan revealed metastatic foci on right cervical area. He was successfully treated with modified radical neck dissection.
Adult
;
Bone Diseases
;
Humans
;
Hypercalcemia
;
Hyperparathyroidism
;
Hyperparathyroidism, Primary
;
Neck
;
Neck Dissection
;
Parathyroid Glands
;
Parathyroid Neoplasms*
;
Technetium Tc 99m Sestamibi*
5.Bezoar-induced Small Bowel Obstruction.
Se Heon OH ; Hwan NAMGUNG ; Mi Hyun PARK ; Dong Guk PARK
Journal of the Korean Society of Coloproctology 2012;28(2):89-93
PURPOSE: The aim of this study was to observe the clinical features of a bezoar-induced small bowel obstruction and to investigate the role of abdominal computed tomography (CT) in establishing the diagnosis. METHODS: We retrospectively reviewed 20 cases of bezoar-induced small bowel obstruction in our hospital from 1996 to 2010. RESULTS: Thirteen patients (65%) had a history of abdominal surgery. Nine patients (45%) were diagnosed with a bezoar before surgery, seven patients were diagnosed by using abdominal CT, and two patients were diagnosed with a small bowel series. Abdominal CT was performed in 15 patients, and the diagnostic accuracy was 47% (7/15). Surgery revealed ten bezoars in the jejunum and 11 in the ileum. Two patients had bezoars found concurrently in the stomach. Spontaneous removal took place in two patients. An enterotomy and bezoar extraction was performed in 15 patients. Fragmentation and milking, a small bowel resection, and a Meckel's diverticulectomy were performed in one patient each. Early operative treatment was possible (P = 0.036) once the bezoar had been diagnosed by using abdominal CT. There tended to be fewer postoperative complications in patients who were diagnosed with a bezoar by using abdominal CT, but the result was not statistically significant (P = 0.712). CONCLUSION: A preoperative diagnosis of bezoar-induced small bowel obstruction by using clinical features was difficult. Increased use of abdominal CT led to a more accurate diagnosis and to earlier surgery for bezoar-induced small bowel obstructions, thereby reducing the rate of complications.
Bezoars
;
Humans
;
Hypogonadism
;
Ileum
;
Jejunum
;
Milk
;
Mitochondrial Diseases
;
Ophthalmoplegia
;
Postoperative Complications
;
Retrospective Studies
;
Stomach
6.The Relationship between Single Nucleotide Polymorphisms of the Carbonic Anhydrase 6 Gene andPhenylthiocarbamide Taste Sensitivity, Taste Disorder.
Jae Woong BAE ; Un Kyung KIM ; Se kyung OH ; Mee Ra RHYU ; Seung Heon SHIN ; Mi Kyung YE
Korean Journal of Otolaryngology - Head and Neck Surgery 2012;55(3):155-160
BACKGROUND AND OBJECTIVES: The phenylthiocarbamide (PTC) and 6-n-propylthiouracil (PROP) taste sensitivity varies among individuals. Recently, it is reported that PROP taste responsiveness is associated with carbonic anhydrase 6 (CA6) gene polymorphism. The CA6 gene, a zinc metalloprotein in human saliva, is affected in taste function and might be correlated with gustatory diversity. The aim of this study was to examine whether PTC taste sensitivity and taste disorder is associated with the CA6 gene polymorphism rs2274327 (C/T), rs2274328 (A/C), and rs2274333 (A/G). SUBJECTS AND METHOD: A total of 217 healthy normal subjects were recruited as controls, and 50 taste disorder patients were recruited as experimental group. The polymorphisms of CA6 gene were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis. All statistical analyses were calculated using the statistical package for the social science software. Haplotypes were estimated by Haploveiw and the PHASE programs. RESULTS: The CA6 gene polymorphisms showed association with taste disorder but not with PTC sensitivity (taster/nontaster). The number of control subjects carrying AA genotype of single nucleotide polymorphism rs2274328 (A/C) in the CA6 gene was higher than the number of the subjects with taste disorder (p=0.048). However, there was no association between controls and taste disorder subjects in the haplotype analysis. CONCLUSION: These data suggest that the CA6 gene polymorphism rs2274328 could affect taste function impairment in patients with taste disorder. This observation requires a further functional study of gustin protein to clarify the association of the CA6 gene polymorphisms with the taste disorder and sensitivity.
Carbon
;
Carbonic Anhydrases
;
Factor IX
;
Genes, vif
;
Genotype
;
Haplotypes
;
Humans
;
Lifting
;
Phenylthiourea
;
Polymorphism, Single Nucleotide
;
Saliva
;
Social Sciences
;
Taste Disorders
;
Zinc
7.DISC-GMCSF (GM-CSF Gene Transduced Defective Infectious Single Cycle Herpes Virus) Gene Therapy in the Established SCCVII squamous cell carcinoma model.
Se Heon KIM ; Eun Chang CHOI ; Won Pyo HONG ; Hyun Chul YOON ; Jong Seok OH
Korean Journal of Otolaryngology - Head and Neck Surgery 2001;44(2):177-183
BACKGROUND AND OBJECTIVES: Over the past five years, preclinical studies evaluating viral-vector-mediated gene transfer in the treatment of head and neck cancer have reported promising results. The main goal of this study was to evaluate the efficacy of intra-tumoral treatment with DISC virus (defective infectious single cycle herpes virus) carrying a GM-CSF immunomodulatory genes as cancer gene therapy. MATERIALS AND METHOD: Using the SCCVII squamous cell carcinoma model, we determined the in vivo GM-CSF production by ELISA method and evaluated the in vivo effects of DISC-GMCSF on the established tumor model. One of the following preparations was injected every two days to a total of three-doses intratumorally: group I (control): PBS alone, group II: heat inactivated DISC-GMCSF, group III: DISC-GMCSF. To prove the specific anti-tumor response, we analyzed the surface phenotype of tumor infiltrating cells. A flow cytometric analysis was performed using FACScan. RESULTS: Clinically relevant gene transfer could be possible by direct injection of tumors in situ. Intratumoral injection of the DISC-GMCSF (GM-CSF gene transduced defective infectious single cycle herpes virus) resulted in effective in vivo production of cytokines (4,500 pg/0.5 g tumor tissue), but as the progeny of the DISC virus could not be infected by other host cells, the effective cytokine production time seems limited. However, the DISC vector was safe and also allowed to be administered repeated treatments as needed. Effects of DISC-GMCSF injection on tthe established syngeneic tumor model showed the greatest inhibition of tumor growth in the DISC-GMCSF treated group (control vs DISC-GMCSF, p<0.01). In contrast to this, heat inactivated DISC-GMCSF showed no significant tumor suppression effect. It was interesting to note from our in vivo analysis of tumor infiltrating cells that treatment of flank tumors with the DISC-GMCSF virus resulted in a significant increase in the percentage of CD8 positive cells within the tumor (control vs DISC-GMCSF, p<0.05). CONCLUSION: Intratumoral injection of DISC-GMCSF significantly suppressed the tumor growth in established tumor model and induced intratumoral increase in immune effector cells. The results not only confirmed clinically relevant gene transfer but also demonstrated the gene transfer to be an effective anti-cancer therapy.
Carcinoma, Squamous Cell*
;
Cytokines
;
Enzyme-Linked Immunosorbent Assay
;
Genes, Neoplasm
;
Genetic Therapy*
;
Granulocyte-Macrophage Colony-Stimulating Factor
;
Head and Neck Neoplasms
;
Hot Temperature
;
Phenotype
8.The Usability of p16 Immunohistochemical Staining as a Screening Test of Human Papillomavirus Infection in Head and Neck Squamous Cell Carcinoma.
Moon Oh KWON ; Se Heon KIM ; Jun Yeon HWANG ; Sei Young LEE
Korean Journal of Otolaryngology - Head and Neck Surgery 2010;53(10):620-626
BACKGROUND AND OBJECTIVES: Although human papillomavirus (HPV) infection has been recognized as having an important role in the pathogenesis of head and neck squamous cell carcinoma (HNSCC), there is no simple and reliable screening test of HPV infection in HNSCC to date. In the present study, we investigated the usability of p16 immunohistochemical staining as a screening test for HPV infection in HNSCC. SUBJECTS AND METHOD: Paraffin blocks were obtained from 45 tonsil SCC patients, who underwent surgery as an initial treatment between 1995 and 2004. Paraffin blocks were also obtained from 20 normal controls. Expression of p16 was investigated immunohistochemically and these results were compared with results of HPV genotyping and the real-time polymerase chain reaction (PCR) assay of viral oncoprotein to validate p16 staining as a reliable diagnostic tool of HPV infection. RESULTS: The expression of p16 was found to be significantly different between tonsil SCC (32/45, 71.1%) and normal control tissues (0/20, 0%)(p<0.05). Among the 45 patients, HPV genotyping and real-time PCR assay were performed in 21 of the 32 p16 (+) patients and in 6 patients of the 13 p16 (-) patients. HPV-16 was detected in 18 (85.7%) out of the 21 p16 (+) patients and in 2 (33.3%) out of the 6 p16 (-) patients. The p16 expression and HPV-16 status was significantly correlated (p<0.05). The two patients who had HPV-16 infection but did not have p16 expression showed an episomal infection or very low viral copy number each. CONCLUSION: The p16 expression may be an useful marker of HPV infection in HNSCC and may be used as a screening test for HPV infection in HNSCC.
Carcinoma, Squamous Cell
;
Coat Protein Complex I
;
Head
;
Human papillomavirus 16
;
Humans
;
Immunohistochemistry
;
Mass Screening
;
Neck
;
Palatine Tonsil
;
Papillomavirus Infections
;
Paraffin
;
Real-Time Polymerase Chain Reaction
9.The Prevalence Rate of Tuberculin Skin Test Positive by Contacts Group to Predict the Development of Active Tuberculosis After School Outbreaks.
Hee Jin KIM ; Byung Chul CHUN ; AmyM KWON ; Gyeong Ho LEE ; Sungweon RYU ; Soo Yeon OH ; Jin Beom LEE ; Se Hwa YOO ; Eui Sook KIM ; Je Hyeong KIM ; Chol SHIN ; Seung Heon LEE
Tuberculosis and Respiratory Diseases 2015;78(4):349-355
BACKGROUND: The tuberculin skin test (TST) is the standard tool to diagnose latent tuberculosis infection (LTBI) in mass screening. The aim of this study is to find an optimal cut-off point of the TST+ rate within tuberculosis (TB) contacts to predict the active TB development among adolescents in school TB outbreaks. METHODS: The Korean National Health Insurance Review and Assessment database was used to identify active TB development in relation to the initial TST (cut-off, 10 mm). The 7,475 contacts in 89 schools were divided into two groups: Incident TB group (43 schools) and no incident TB group (46 schools). LTBI treatment was initiated in 607 of the 1,761 TST+ contacts. The association with active TB progression was examined at different cut-off points of the TST+ rate. RESULTS: The mean duration of follow-up was 3.9+/-0.9 years. Thirty-three contacts developed active TB during the 4,504 person-years among the TST+ contacts without LTBI treatment (n=1,154). The average TST+ rate for the incident TB group (n=43) and no incident TB group (n=46) were 31.0% and 15.5%, respectively. The TST+ rate per group was related with TB progression (odds ratio [OR], 1.025; 95% confidence interval [CI], 1.001-1.050; p=0.037). Based on the TST+ rate per group, active TB was best predicted at TST+ > or = 16% (OR, 3.11; 95% CI, 1.29-7.51; area under curve, 0.64). CONCLUSION: Sixteen percent of the TST+ rate per group within the same grade students can be suggested as an optimal cut-off to predict active TB development in middle and high schools TB outbreaks.
Adolescent
;
Area Under Curve
;
Disease Outbreaks*
;
Follow-Up Studies
;
Humans
;
Latent Tuberculosis
;
Mass Screening
;
National Health Programs
;
Prevalence*
;
Skin Tests*
;
Skin*
;
Tuberculin Test
;
Tuberculin*
;
Tuberculosis*
10.Dose-Incidence Relationships on the Prenatal Effects of Gamma-Radiation in Mice.
Dae Won BANG ; Jong Hwan LEE ; Heon OH ; Se Ra KIM ; Tae Hwan KIM ; Yun Sil LEE ; Cha Soo LEE ; Sung Ho KIM
Journal of Veterinary Science 2002;3(1):7-11
The objective of this investigation was to evaluate dose-incidence relationships on the prenatal effects of gamma-radiation. Pregnant ICR mice were exposed on day 11.5 after conception, coincident with the most sensitive stage for the induction of major congenital malformations, with 0.5-4.0 Gy of gamma-radiations. The animals were sacrificed on day 18 of gestation and the fetuses were examined for mortality, growth retardation, change in head size and any other morphological abnormalities. With increasing radiation dose, incidence of small head, growth retarded fetuses, cleft palate, dilatation of cerebral ventricle and abnormalities of the extremities in live fetuses rose. The threshold doses of radiation that induced cleft palate and dilatation of cerebral ventricle, and abnormal extremities were between 1.0 and 2.0 Gy, and between 0.5 and 1.0 Gy, respectively.
Animals
;
Bone and Bones/*abnormalities/radiation effects
;
Congenital Abnormalities/embryology/epidemiology/*radionuclide imaging
;
Female
;
Fetal Death
;
Fetal Resorption/epidemiology/radionuclide imaging
;
*Gamma Rays
;
Incidence
;
Mice
;
Mice, Inbred ICR
;
Pregnancy
;
*Prenatal Exposure Delayed Effects
;
*Whole-Body Irradiation