This study was conducted to understand the dynamics of microbial communities of soil microorganisms, and their distribution and abundance in the indigenous microorganisms (IMOs) manipulated from humus collected from the forest near the crop field. The soil microorganisms originated from humus and artificially cultured microbial-based soil amendments were characterized by molecular and biochemical analyses. The bacterial population (2 × 106∼13 × 106 CFU/g sample) was approximately 100-fold abundant than the fungal population (2 × 104∼8 × 104 CFU/g sample). The 16S rDNA and ITS sequence analyses showed that the bacterial and fungal communities in humus and IMOs were mainly composed of Bacillus and Pseudomonas, and Trichoderma and Aspergillus species, respectively. Some of the bacterial isolates from the humus and IMOs showed strong inhibitory activity against soil-borne pathogenic fungi Fusarium oxysporum and Sclerotinia sclerotiorum. These bacteria also showed the siderophore production activity as well as phosphate solubilizing activity, which are requisite traits for biological control of plant pathogenic fungi. These results suggest that humus and IMOs could be a useful resource for sustainable agriculture.

PURPOSE: The present study was conducted to identify the correlation between control grade and stereoacuity in basic intermittent exotropia (X[T]). METHODS: Eighty-six patients with basic X (T) were divided into 3 subgroups according to their control grade and the near and distant stereoacuities were evaluated. RESULTS: Group 1; good control group, consisted of 28 patients, group 2; fair control, 30 patients, and group 3; poor control, 28 patients. Mean near stereoacuities measured by the Titmus test were 58.21 arcseconds in group 1, 75.33 arcseconds in group 2, and 151.43 arcseconds in group 3. The mean distant stereoacuities measured by a Mentor B-VAT(R) II-SG videoacuity tester were 118.93 arcseconds in group 1, 165.33 arcseconds in group 2, and 276.43 arcseconds in group 3. When comparing the 3 groups, the poorer the control grade, the worse were the near and distant stereoacuities (p = 0.002, p < 0.001). When compared in pairs, however, group 1 showed a better near stereoacuity than groups 2 and 3 (p = 0.02, p = 0.002, respectively), while group 2 and group 3 did not show any distinct differences (p = 0.13). Group 1 also showed a better distant stereoacuity than groups 2 and 3 (p = 0.02, p < 0.001, respectively), as did group 2 compared to group 3 (p < 0.001). CONCLUSIONS: Control grade and distant stereoacuity have significant correlation in patients with basic X (T) and can function as helpful indicators for monitoring the deterioration and progression of exodeviation.
Exotropia ; Humans ; Mentors

No abstract available.
Chondroma* ; Humans ; Young Adult*

No abstract available.
Fractures, Open*

Onion (Allium cepa L.) is one of the major vegetable crops in Korea that are damaged and lost by pathogenic fungal infection during storage due to a lack of proper storage conditions. The aim of this study was to determine an appropriate control measure using thymol to increase the shelf life of onions. To control fungal infections that occur during low-temperature storage, it is necessary to identify the predominant fungal pathogens that appear in low-temperature storage houses. Botrytis aclada was found to be the most predominant fungal pathogen during low-temperature storage. The antifungal activity of the plant essential oil thymol was tested and compared to that of the existing sulfur treatments. B. aclada growth was significantly inhibited up to 16 weeks with spray treatments using a thymol solution. To identify an appropriate method for treating onions in a low-temperature storage house, thymol was delivered by two fumigation treatment methods, either by heating it in the granule form or as a solution at low-temperature storage conditions (in vivo). We confirmed that the disease severity was reduced up to 96% by fumigating thymol solution compared to the untreated control. The efficacy of the fumigation of thymol solution was validated by testing onions in a low-temperature storage house in Muan, Jeollanam-do. Based on these results, the present study suggests that fumigation of the thymol solution as a natural preservative and fungicide can be used as an eco-friendly substitute for existing methods to control postharvest disease in long-term storage crops on a commercial scale.
Botrytis ; Fumigation* ; Heating ; Hot Temperature ; Jeollanam-do ; Korea ; Methods ; Onions* ; Plants ; Sulfur ; Thymol* ; Vegetables

Several postharvest diseases of table grapes (Vitis vinifera) occur during storage, and gray mold rot is a particularly severe disease because the causal agent, Botrytis cinerea, grows at temperatures as low as 0degrees C. Other postharvest diseases, such as those caused by Penicillium spp. and Aspergillus spp., also often lead to deterioration in the quality of table grapes after harvest. The use of plant essential oils such as thymol and linalool, to reduce postharvest diseases in several kinds of fruits, including table grapes and oranges, has received much attention in European countries. However, to the best of our knowledge there has been no report of the use of thymol fumigation to control gray mold in table grapes in Korea. Thymol (30 microg/mL) and linalool (120 microg/mL) significantly inhibited mycelial growth and conidia germination of B. cinerea. The occurrence rate of gray mold rot of B. cinerea and other unknown fungi was significantly reduced by fumigation with 30 microg/mL thymol in several table grape cultivars, such as Campbell early, Muscat Bailey A, Sheridan, and Geobong. In this study, fumigation with 30 microg/mL thymol, had no influence on the sugar content and hardness of grapes, but reduced fungal infection significantly. This suggests that 30 microg/mL thymol could be utilized to reduce deterioration of grapes due to gray mold and other fungal infections during long-term storage.
Aspergillus ; Botrytis ; Citrus sinensis ; Fruit ; Fumigation* ; Fungi ; Germination ; Hardness ; Korea ; Oils, Volatile ; Oman ; Penicillium ; Plants ; Spores, Fungal ; Thymol* ; Vitis*

Thirty-six femoral heads with non-traumatic osteonecrosis(ON) at precollapse stage in 32 patients were studied with repeated magnetic resonance(MR) imagings at three-month intervals to reveal the occurrence of marrow edema of the proximal femur in early stage ON. Eighteen hips were randomly selected for the core decompression and the remaining 18 hips were treated conservatively. The criteria of marrow edema in this study were signal abnormalities of low signal intensity in T1-weighted image, and isointensity or hyperintensity in T2-weighted image which involved the femoral head beyond the necrotic zone, extending to the neck and trochanteric area. Eight femoral heads demonstrated marrow edema at the initial MR imaging and seven out of eight were associated with pain for one to six weeks before the first MR study. Core decompression was performed on four out of those seven symptomatic femoral heads with marrow edema after the first MR imaging. The symptom was relieved promptly in three out of four core decompressed hips. The remaining one core decompressed case showed reccurrent marow edema on follow-up MR imagings. Out of 28 hips free of marrow edema at the initial MR study, 14 hips underwent core decompression after the first evaluation and the remaining 14 hips were treated conservatively. One out of 14 core-decompressed hips and three out of 14 nonoperated hips showed marrow edema in follow-up MR studies. Two cases experienced hip pain while two cases remained symptomless at the presence of marrow edema. Four femoral heads under-went core biopsy and three cases received total hip arthroplasty at the presence of marrow edema. In those four hips which underwent core decompression during the presence of marrow edema, intraosseous pressure(IOP) ranged from 36 to 60 (mean; 49) mmHg. In the remaining 14 core decompressed hips, the IOP ranged from 16 to 52 (mean; 37) mmHg. The histology of those four core biopsies and three resected femoral heads showed a collection of eosinophilic plasma-like fluid in the marrow space around the necrotic zone, a finding compatible with marrow edema. This study suggests that marrow edema of the proximal femur may occur during the progression of the ON of the femoral head and is frequently associated with increased intraosseous pressure and pain. Core decompression can relieve marrow edema and associated pain promptly. But the effect is temporary, and core decompression does not prevent marrow edema permanently.
Arthroplasty, Replacement, Hip ; Biopsy ; Bone Marrow ; Decompression ; Edema ; Eosinophils ; Femur ; Follow-Up Studies ; Head ; Hip ; Humans ; Magnetic Resonance Imaging ; Neck ; Osteonecrosis ; Prospective Studies

Twenty-eight patients with 20 tibia fractures and 8 femur fractures were treated with external fixation. The average age at fracture was 10 years 10 months ranging from 5 years to 17 years 6 months. Of 28 fractures, 6 were closed fractures and 22 were open fractures. The average follow-up of these children was 23 months. Monofixators were used in 12 fractures and Ilizarov fixators in 16 fractures. The average time to healing of the fractures was 14.6 weeks(range, 6 to 44 weeks). Seven segmental bone defects(range, 2 to 17cm) were treated with the Ilizarov method of internal bone transport using the transport ring and bone grafting at the docking site. The average healing index for callus distraction was 25 days per centimeter. Seven patients had 10 major complications that necessitated additional operative procedures. There were 4 nonunions. Three patients had an infected nonunion, which was treated with the Ilizarov fixator and polymethyl-methacrylate antibiotic beads. One patient had a hypertrophic nonunion which was treated with plate fixation. Three patients who had an epiphyseal injury had shortening with angular deformity, which was treated by callus distraction. Three patients had a joint contracture, which was treated by the percutaneous tenotomy and Ilizarov fixator. The selection of the type of external fixator depends on the fracture pattern and the Ilizarov fixator is recommended for complicated fracture with severe comminution or segmental bone loss.
Bone Transplantation ; Bony Callus ; Child ; Congenital Abnormalities ; Contracture ; External Fixators ; Femur ; Follow-Up Studies ; Fractures, Bone ; Fractures, Closed ; Fractures, Open ; Humans ; Ilizarov Technique ; Joints ; Surgical Procedures, Operative ; Tenotomy ; Tibia

The history of the radiological examination of the breast has been long, and recently the methods forexamining the breast are variable: mammography, thermography, xeroradiography, ultrasonography, water-pathsonography, transillumination, mammary C.T., M.R. Imaging, infra-red scanning, nuclear medicine, andgalactography. Most of these methods were not proven or have not been satisfied on their clinical testing butconventional mammography has been the gold standard for diagnosis of the breast cancer and no other method hasreached its effectiveness. The authors performed mammography in 488 patiens with breast abnormalities who hadvisited to Yeung Nam University hospital form May, 1983 to Dec. 1984. And we analyzed the mammographic findingsand diagnostic accuracy in pathologically confirmed 53 cases by operations or biopsies. The results are asfollows: 1. Among 16 cases of breast malignancies, (1) the most prevalent age group is 6th decade(37.5%), followedby 5th decade(31.3%), (2) the most common pathologic type is ductal carcinoma(93.75%), (3) the masses are largerthan 2cm, in 8 cases, smaller than 2cm. in 5 cases, and no visible mass is observed in 3 cases. 2. Among 37 casesof benign breast diseases, (1) the most prevalent age group is 4th & 5th decades(each11 cases), and followed by3rd decade(8 cases), (2) the most common disease is benign tumor (17 casees), followed by fibrocystic dysplasia(13cases), and inflammating disease(7 cases). (3) radiologically visible masses are observed in 16 cases and themasses are smaller than 2cm. in 11 cases. 3. the diagnostic accuracy of mammography compared with that ofpathology, (1) in malignancy, the accuracy rate is 75%(12/16 cases), and 87%(32/37 cases) in benign diseases. (2)avearage accuracy rate is 81%(44/53 cases).
Biopsy ; Breast Diseases ; Breast Neoplasms ; Breast ; Diagnosis ; Humans ; Mammography ; Methods ; Nuclear Medicine ; Thermography ; Transillumination ; Ultrasonography ; Xeroradiography

The seed borne disease such as bakanae is difficult to control. Crop yield loss caused by bakanae depending on the regions and varieties grown, ranging from 3.0% to 95.4%.Bakanae is an important disease of rice worldwide and the pathogen was identified as Fusarium fujikuroi Nirenberg (teleomorph: Gibberella fujikuroi Sawada). Currently, four Fusaria (F. fujikuroi, F. proliferatum, F. verticillioides and F. andiyazi) belonging to F. fujikuroi species complex are generally known as the pathogens of bakanae. The infection occurs through both seed and soil-borne transmission. When infection occurs during the heading stage, rice seeds become contaminated. Molecular detection of pathogens of bakanae is important because identification based on morphological and biological characters could lead to incorrect species designation and time-consuming. Seed disinfection has been studied for a long time in Korea for the management of the bakanae disease of rice. As seed disinfectants have been studied to control bakanae, resistance studies to chemicals have been also conducted. Presently biological control and resistant varieties are not widely used. The detection of this pathogen is critical for seed certification and for preventing field infections. In South Korea, bakanae is designated as a regulated pathogen. To provide highly qualified rice seeds to farms, Korea Seed & Variety Service (KSVS) has been producing and distributing certified rice seeds for producing healthy rice in fields. Therefore, the objective of the study is to summarize the recent progress in molecular identification, fungicide resistance, and the management strategy of bakanae.